(Circular) (Six-base) MAPSORT of: pFN21AB6841.seq Check: 8679 from: 1 to: 5221 pFN21AB6841 5221 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2307 2382 2307 Size: 75 5146 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3031 278 Size: 53 83 186 936 1495 2468 Fragments arranged by size: 2468 1495 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2564 1937 Size: 627 4594 Acc65I G'GTAC_C Cuts at: 2542 2542 Size: 5221 AclI AA'CG_TT Cuts at: 1563 3348 3721 1563 Size: 1785 373 3063 Fragments arranged by size: 3063 1785 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3284 4668 4936 1632 Size: 1652 1384 268 1917 Fragments arranged by size: 1917 1652 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5024 AflIII A'CryG_T Cuts at: 1203 2012 4120 5097 1203 Size: 809 2108 977 1327 Fragments arranged by size: 2108 1327 977 809 AgeI A'CCGG_T Cuts at: 4052 4052 Size: 5221 AhdI GACnn_n'nnGTC Cuts at: 2464 3950 2464 Size: 1486 3735 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5221 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5221 AlwNI CAG_nnn'CTG Cuts at: 2163 2383 2964 4536 2163 Size: 220 581 1572 2848 Fragments arranged by size: 2848 1572 581 220 ApaLI G'TGCA_C Cuts at: 3278 4434 3278 Size: 1156 4065 ApoI r'AATT_y Cuts at: 1493 2530 2756 2908 5017 1493 Size: 1037 226 152 2109 1697 Fragments arranged by size: 2109 1697 1037 226 152 AseI AT'TA_AT Cuts at: 160 1032 3775 160 Size: 872 2743 1606 Fragments arranged by size: 2743 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5221 AvaI C'yCGr_G Cuts at: 1870 1943 2304 2408 2504 2546 1870 Size: 73 361 104 96 42 4545 Fragments arranged by size: 4545 361 104 96 73 42 BamHI G'GATC_C Cuts at: 2551 2918 2551 Size: 367 4854 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2423 2482 2542 3997 618 Size: 325 217 627 636 59 60 1455 1842 Fragments arranged by size: 1842 1455 636 627 325 217 60 59 BanII G_rGCy'C Cuts at: 1391 2217 2409 2505 2540 1391 Size: 826 192 96 35 4072 Fragments arranged by size: 4072 826 192 96 35 BbeI G_GCGC'C Cuts at: 2427 2486 2427 Size: 59 5162 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4250 BbvCI CC'TCA_GC Cuts at: 2327 2327 Size: 5221 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2261 2295 3412 3446 1903 Size: 34 324 34 1117 34 3678 Fragments arranged by size: 3678 1117 324 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3113 4323 3113 Size: 1210 4011 BclI T'GATC_A Cuts at: 1548 1548 Size: 5221 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2480 3832 136 Size: 107 122 71 631 605 808 1352 1525 Fragments arranged by size: 1525 1352 808 631 605 122 107 71 BglII A'GATC_T Cuts at: 5216 5216 Size: 5221 BlpI GC'TnA_GC Cuts at: 2638 2638 Size: 5221 Bme1580I G_kGCm'C Cuts at: 1790 3282 4438 1790 Size: 1492 1156 2573 Fragments arranged by size: 2573 1492 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5221 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2952 3910 442 Size: 416 53 83 399 24 1535 958 1753 Fragments arranged by size: 1753 1535 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5221 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2408 3881 1239 Size: 1169 1473 2579 Fragments arranged by size: 2579 1473 1169 Bpu10I CC'TnA_GC Cuts at: 2327 2327 Size: 5221 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3346 4211 4509 3346 Size: 865 298 4058 Fragments arranged by size: 4058 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2286 3884 882 Size: 617 787 1598 2219 Fragments arranged by size: 2219 1598 787 617 BsaAI yAC'GTr Cuts at: 493 1204 3012 493 Size: 711 1808 2702 Fragments arranged by size: 2702 1808 711 BsaBI GATnn'nnATC Cuts at: 5215 5215 Size: 5221 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 2424 2483 3028 Size: 53 83 186 936 600 291 59 545 Cuts at: 3028 3410 4045 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 600 545 382 291 186 83 59 53 BsaWI w'CCGG_w Cuts at: 3654 4052 4326 4473 4884 3654 Size: 398 274 147 411 3991 Fragments arranged by size: 3991 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4996 Fragments arranged by size: 4996 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2522 1186 Size: 1336 3885 BseYI C'CCAG_C Cuts at: 2142 2433 2492 4424 4920 2142 Size: 291 59 1932 496 2443 Fragments arranged by size: 2443 1932 496 291 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4648 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2421 2671 3432 4460 1540 Size: 275 186 420 250 761 1028 2301 Fragments arranged by size: 2301 1028 761 420 275 250 186 BsiHKAI G_wGCw'C Cuts at: 3282 3367 4438 3282 Size: 85 1071 4065 Fragments arranged by size: 4065 1071 85 BsmI GAATG_Cn' Cuts at: 2453 2732 2825 2453 Size: 279 93 4849 Fragments arranged by size: 4849 279 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2003 2333 2003 Size: 330 4891 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2409 2505 2540 3282 3367 4438 Size: 399 427 192 96 35 742 85 1071 Cuts at: 4438 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 427 399 192 96 85 35 BspEI T'CCGG_A Cuts at: 4884 4884 Size: 5221 BspHI T'CATG_A Cuts at: 3110 4840 3110 Size: 1730 3491 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2307 2382 2576 844 Size: 1463 75 194 3489 Fragments arranged by size: 3489 1463 194 75 BsrBI CCG'CTC Cuts at: 1394 1823 2248 3108 1394 Size: 429 425 860 3507 Fragments arranged by size: 3507 860 429 425 BsrDI GCAATG_nn' Cuts at: 65 1248 3716 3890 65 Size: 1183 2468 174 1396 Fragments arranged by size: 2468 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2020 2420 3865 4052 1700 Size: 110 210 400 1445 187 2869 Fragments arranged by size: 2869 1445 400 210 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5221 BssHII G'CGCG_C Cuts at: 1925 2153 2282 1925 Size: 228 129 4864 Fragments arranged by size: 4864 228 129 BssSI C'ACGA_G Cuts at: 3281 4293 3281 Size: 1012 4209 BstAPI GCAn_nnn'nTGC Cuts at: 794 2163 2185 2257 2383 794 Size: 1369 22 72 126 3632 Fragments arranged by size: 3632 1369 126 72 22 BstBI TT'CG_AA Cuts at: 4025 4025 Size: 5221 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5221 BstYI r'GATC_y Cuts at: 1972 2551 2918 3303 3320 4772 4858 4870 5216 Size: 579 367 385 17 1452 86 12 346 Cuts at: 5216 1972 Size: 1977 Fragments arranged by size: 1977 1452 579 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2111 2111 Size: 5221 BtgI C'CryG_G Cuts at: 513 1065 2004 2096 513 Size: 552 939 92 3638 Fragments arranged by size: 3638 939 552 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4482 Fragments arranged by size: 4482 683 56 BtsI GCAGTG_nn' Cuts at: 2737 3531 3551 2737 Size: 794 20 4407 Fragments arranged by size: 4407 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5221 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5186 DraI TTT'AAA Cuts at: 2525 2525 Size: 5221 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4228 809 Size: 507 2912 1802 Fragments arranged by size: 2912 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2418 2668 3557 Size: 54 1425 50 275 18 588 250 889 Cuts at: 3557 5186 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 588 275 250 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2418 2668 1537 Size: 275 606 250 4090 Fragments arranged by size: 4090 606 275 250 EarI CTCTTCn'nnn_ Cuts at: 1353 2482 3151 1353 Size: 1129 669 3423 Fragments arranged by size: 3423 1129 669 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2039 2459 3808 4182 4328 387 Size: 1064 138 450 420 1349 374 146 1280 Fragments arranged by size: 1349 1280 1064 450 420 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2408 3284 3881 4668 4936 1239 Size: 393 776 876 597 787 268 1524 Fragments arranged by size: 1524 876 787 776 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2079 2104 2079 Size: 25 5196 EcoRI G'AATT_C Cuts at: 2530 2908 2530 Size: 378 4843 FspI TGC'GCA Cuts at: 2054 2167 3727 2054 Size: 113 1560 3548 Fragments arranged by size: 3548 1560 113 HaeII r_GCGC'y Cuts at: 1678 2208 2326 2395 2427 2486 4368 1678 Size: 530 118 69 32 59 1882 2531 Fragments arranged by size: 2531 1882 530 118 69 59 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2450 2482 3862 3894 3936 Size: 32 135 32 878 32 1380 32 42 Cuts at: 3936 3968 1373 Size: 32 2626 Fragments arranged by size: 2626 1380 878 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2565 2811 669 Size: 988 281 627 246 3079 Fragments arranged by size: 3079 988 627 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5221 HpaI GTT'AAC Cuts at: 2811 2811 Size: 5221 KasI G'GCGC_C Cuts at: 2423 2482 2423 Size: 59 5162 KpnI G_GTAC'C Cuts at: 2546 2546 Size: 5221 MfeI C'AATT_G Cuts at: 2820 2820 Size: 5221 MluI A'CGCG_T Cuts at: 2012 5097 2012 Size: 3085 2136 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4335 4519 1305 Size: 3030 184 2007 Fragments arranged by size: 3030 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3399 Fragments arranged by size: 3399 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2173 3179 3538 3697 518 Size: 648 1007 1006 359 159 2042 Fragments arranged by size: 2042 1007 1006 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2098 2160 2197 2254 2637 3314 4462 4707 Size: 492 62 37 57 383 677 1148 245 Cuts at: 4707 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 492 383 245 62 57 37 NaeI GCC'GGC Cuts at: 2422 2422 Size: 5221 NarI GG'CG_CC Cuts at: 2424 2483 2424 Size: 59 5162 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3730 Fragments arranged by size: 3730 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5221 NgoMIV G'CCGG_C Cuts at: 2420 2420 Size: 5221 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5221 NotI GC'GGCC_GC Cuts at: 2668 2668 Size: 5221 NspI r_CATG'y Cuts at: 1132 1229 2579 4124 1132 Size: 97 1350 1545 2229 Fragments arranged by size: 2229 1545 1350 97 PasI CC'CwG_GG Cuts at: 1348 2065 1348 Size: 717 4504 PciI A'CATG_T Cuts at: 4120 4120 Size: 5221 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5221 PmeI GTTT'AAAC Cuts at: 2525 2525 Size: 5221 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3290 3322 4803 4835 3290 Size: 32 1481 32 3676 Fragments arranged by size: 3676 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2079 2104 2079 Size: 25 5196 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5221 PsiI TTA'TAA Cuts at: 2791 2791 Size: 5221 PspXI vC'TCGA_Gb Cuts at: 2408 2408 Size: 5221 PstI C_TGCA'G Cuts at: 830 2379 2573 830 Size: 1549 194 3478 Fragments arranged by size: 3478 1549 194 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5221 SacII CC_GC'GG Cuts at: 2099 2099 Size: 5221 SalI G'TCGA_C Cuts at: 1936 2563 1936 Size: 627 4594 SanDI GG'GwC_CC Cuts at: 2079 2104 2079 Size: 25 5196 SbfI CC_TGCA'GG Cuts at: 2379 2573 2379 Size: 194 5027 ScaI AGT'ACT Cuts at: 2183 3469 5181 2183 Size: 1286 1712 2223 Fragments arranged by size: 2223 1712 1286 SfcI C'TryA_G Cuts at: 826 1045 2375 2569 3704 4385 4576 826 Size: 219 1330 194 1135 681 191 1471 Fragments arranged by size: 1471 1330 1135 681 219 194 191 SfoI GGC'GCC Cuts at: 2425 2484 2425 Size: 59 5162 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5221 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5221 SmaI CCC'GGG Cuts at: 1872 2306 2548 1872 Size: 434 242 4545 Fragments arranged by size: 4545 434 242 SmlI C'TyrA_G Cuts at: 820 1017 1943 2408 3325 4226 4488 820 Size: 197 926 465 917 901 262 1553 Fragments arranged by size: 1553 926 917 901 465 262 197 SnaBI TAC'GTA Cuts at: 493 3012 493 Size: 2519 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5221 SphI G_CATG'C Cuts at: 1132 2579 1132 Size: 1447 3774 SspI AAT'ATT Cuts at: 5 52 3145 5 Size: 47 3093 2081 Fragments arranged by size: 3093 2081 47 StuI AGG'CCT Cuts at: 1517 2399 2474 1517 Size: 882 75 4264 Fragments arranged by size: 4264 882 75 StyI C'CwwG_G Cuts at: 513 1065 2004 2469 2660 513 Size: 552 939 465 191 3074 Fragments arranged by size: 3074 939 552 465 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3248 3265 3418 3603 4048 325 Size: 1255 1668 17 153 185 445 1498 Fragments arranged by size: 1668 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 3467 5179 Size: 274 80 33 51 1443 204 1286 1712 Cuts at: 5179 96 Size: 138 Fragments arranged by size: 1712 1443 1286 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2123 2155 5181 5213 2123 Size: 32 3026 32 2131 Fragments arranged by size: 3026 2131 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5221 XbaI T'CTAG_A Cuts at: 2557 2557 Size: 5221 XhoI C'TCGA_G Cuts at: 1943 2408 1943 Size: 465 4756 XmaI C'CCGG_G Cuts at: 1870 2304 2546 1870 Size: 434 242 4545 Fragments arranged by size: 4545 434 242 XmnI GAAnn'nnTTC Cuts at: 3350 3350 Size: 5221 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3029 276 Size: 53 83 186 936 1495 2468 Fragments arranged by size: 2468 1495 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI XcmI