(Circular) (Six-base) MAPSORT of: pFN21AE0638.seq Check: 6226 from: 1 to: 6046 pFN21AE0638 6046 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3856 278 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3171 3389 1937 Size: 1234 218 4594 Fragments arranged by size: 4594 1234 218 Acc65I G'GTAC_C Cuts at: 3367 3367 Size: 6046 AclI AA'CG_TT Cuts at: 1563 4173 4546 1563 Size: 2610 373 3063 Fragments arranged by size: 3063 2610 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4109 5493 5761 1632 Size: 2477 1384 268 1917 Fragments arranged by size: 2477 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5849 AflIII A'CryG_T Cuts at: 1203 3063 4945 5922 1203 Size: 1860 1882 977 1327 Fragments arranged by size: 1882 1860 1327 977 AgeI A'CCGG_T Cuts at: 4877 4877 Size: 6046 AhdI GACnn_n'nnGTC Cuts at: 4775 4775 Size: 6046 AleI CACnn'nnGTG Cuts at: 1166 2494 2585 1166 Size: 1328 91 4627 Fragments arranged by size: 4627 1328 91 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6046 AlwNI CAG_nnn'CTG Cuts at: 2819 3789 5361 2819 Size: 970 1572 3504 Fragments arranged by size: 3504 1572 970 ApaI G_GGCC'C Cuts at: 3341 3341 Size: 6046 ApaLI G'TGCA_C Cuts at: 3090 4103 5259 3090 Size: 1013 1156 3877 Fragments arranged by size: 3877 1156 1013 ApoI r'AATT_y Cuts at: 1493 3355 3581 3733 5842 1493 Size: 1862 226 152 2109 1697 Fragments arranged by size: 2109 1862 1697 226 152 AscI GG'CGCG_CC Cuts at: 3070 3070 Size: 6046 AseI AT'TA_AT Cuts at: 160 1032 4600 160 Size: 872 3568 1606 Fragments arranged by size: 3568 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 AvaI C'yCGr_G Cuts at: 1870 1943 2048 2074 2692 3085 3106 3371 1870 Size: 73 105 26 618 393 21 265 4545 Fragments arranged by size: 4545 618 393 265 105 73 26 21 BamHI G'GATC_C Cuts at: 2948 3376 3743 2948 Size: 428 367 5251 Fragments arranged by size: 5251 428 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2080 2372 2563 3367 4822 Size: 325 217 627 293 292 191 804 1455 Cuts at: 4822 618 Size: 1842 Fragments arranged by size: 1842 1455 804 627 325 293 292 217 191 BanII G_rGCy'C Cuts at: 1391 2127 2739 3250 3262 3341 3365 1391 Size: 736 612 511 12 79 24 4072 Fragments arranged by size: 4072 736 612 511 79 24 12 BbeI G_GCGC'C Cuts at: 2084 2084 Size: 6046 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2874 928 Size: 971 975 4100 Fragments arranged by size: 4100 975 971 BbvCI CC'TCA_GC Cuts at: 2114 2114 Size: 6046 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4237 4271 1903 Size: 34 2300 34 3678 Fragments arranged by size: 3678 2300 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3938 5148 3938 Size: 1210 4836 BclI T'GATC_A Cuts at: 1548 1548 Size: 6046 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4657 136 Size: 107 122 71 631 605 2985 1525 Fragments arranged by size: 2985 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6041 6041 Size: 6046 BlpI GC'TnA_GC Cuts at: 2432 3137 3311 3463 2432 Size: 705 174 152 5015 Fragments arranged by size: 5015 705 174 152 Bme1580I G_kGCm'C Cuts at: 1790 2360 2568 3094 3341 4107 5263 1790 Size: 570 208 526 247 766 1156 2573 Fragments arranged by size: 2573 1156 766 570 526 247 208 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6046 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2628 3344 3777 Size: 416 53 83 399 24 1211 716 433 Cuts at: 3777 4735 442 Size: 958 1753 Fragments arranged by size: 1753 1211 958 716 433 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2474 2936 4706 1239 Size: 1235 462 1770 2579 Fragments arranged by size: 2579 1770 1235 462 Bpu10I CC'TnA_GC Cuts at: 2114 2114 Size: 6046 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4171 5036 5334 4171 Size: 865 298 4883 Fragments arranged by size: 4883 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4709 882 Size: 617 3210 2219 Fragments arranged by size: 3210 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2538 2887 3066 3837 493 Size: 711 1334 349 179 771 2702 Fragments arranged by size: 2702 1334 771 711 349 179 BsaBI GATnn'nnATC Cuts at: 6040 6040 Size: 6046 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2081 3853 4235 4870 Size: 53 83 186 936 548 1772 382 635 Cuts at: 4870 275 Size: 1451 Fragments arranged by size: 1772 1451 936 635 548 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2025 2042 2549 4479 4877 5151 5298 5709 2025 Size: 17 507 1930 398 274 147 411 2362 Fragments arranged by size: 2362 1930 507 411 398 274 147 17 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5821 Fragments arranged by size: 5821 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 2273 1186 Size: 892 195 4959 Fragments arranged by size: 4959 892 195 BseYI C'CCAG_C Cuts at: 5249 5745 5249 Size: 496 5550 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2054 1163 Size: 573 318 5155 Fragments arranged by size: 5155 573 318 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3496 4257 5285 1540 Size: 275 186 1495 761 1028 2301 Fragments arranged by size: 2301 1495 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2739 3094 3103 3115 3250 4107 4192 5263 2739 Size: 355 9 12 135 857 85 1071 3522 Fragments arranged by size: 3522 1071 857 355 135 85 12 9 BsmI GAATG_Cn' Cuts at: 2133 3557 3650 2133 Size: 1424 93 4529 Fragments arranged by size: 4529 1424 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2360 2568 2739 3094 3103 3115 Size: 399 337 233 208 171 355 9 12 Cuts at: 3115 3250 3262 3341 3365 4107 4192 5263 1391 Size: 135 12 79 24 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 355 337 233 208 171 135 85 79 24 12 12 9 BspEI T'CCGG_A Cuts at: 2549 5709 2549 Size: 3160 2886 BspHI T'CATG_A Cuts at: 3935 5665 3935 Size: 1730 4316 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3401 844 Size: 2557 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2458 3933 1394 Size: 429 635 1475 3507 Fragments arranged by size: 3507 1475 635 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3291 4541 4715 65 Size: 1183 2043 1250 174 1396 Fragments arranged by size: 2043 1396 1250 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2841 4690 4877 1700 Size: 110 1031 1849 187 2869 Fragments arranged by size: 2869 1849 1031 187 110 BsrGI T'GTAC_A Cuts at: 96 2577 96 Size: 2481 3565 BssHII G'CGCG_C Cuts at: 1925 3070 1925 Size: 1145 4901 BssSI C'ACGA_G Cuts at: 2151 2438 2490 2604 3243 4106 5118 2151 Size: 287 52 114 639 863 1012 3079 Fragments arranged by size: 3079 1012 863 639 287 114 52 BstAPI GCAn_nnn'nTGC Cuts at: 794 2620 794 Size: 1826 4220 BstBI TT'CG_AA Cuts at: 4850 4850 Size: 6046 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6046 BstXI CCAn_nnnn'nTGG Cuts at: 2353 2452 2980 2353 Size: 99 528 5419 Fragments arranged by size: 5419 528 99 BstYI r'GATC_y Cuts at: 1972 2948 3376 3743 4128 4145 5597 5683 5695 Size: 976 428 367 385 17 1452 86 12 Cuts at: 5695 6041 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 976 428 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2258 2258 Size: 6046 BtgI C'CryG_G Cuts at: 513 1065 2004 2709 513 Size: 552 939 705 3850 Fragments arranged by size: 3850 939 705 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2431 2609 2889 487 Size: 683 56 1205 178 280 3644 Fragments arranged by size: 3644 1205 683 280 178 56 BtsI GCAGTG_nn' Cuts at: 2107 2196 2480 2621 3562 4356 4376 2107 Size: 89 284 141 941 794 20 3777 Fragments arranged by size: 3777 941 794 284 141 89 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6046 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6011 DraI TTT'AAA Cuts at: 3350 3350 Size: 6046 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5053 809 Size: 507 3737 1802 Fragments arranged by size: 3737 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2166 2442 2706 Size: 54 1425 50 275 18 336 276 264 Cuts at: 2706 3493 4382 6011 8 Size: 787 889 1629 43 Fragments arranged by size: 1629 1425 889 787 336 276 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3493 1537 Size: 275 1681 4090 Fragments arranged by size: 4090 1681 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3267 3976 1353 Size: 1914 709 3423 Fragments arranged by size: 3423 1914 709 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2636 4633 5007 5153 387 Size: 1064 138 1047 1997 374 146 1280 Fragments arranged by size: 1997 1280 1064 1047 374 146 138 EcoICRI GAG'CTC Cuts at: 2737 3248 2737 Size: 511 5535 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2474 2936 4109 4706 5493 5761 1239 Size: 393 842 462 1173 597 787 268 1524 Fragments arranged by size: 1524 1173 842 787 597 462 393 268 EcoO109I rG'GnC_Cy Cuts at: 2600 3274 3338 2600 Size: 674 64 5308 Fragments arranged by size: 5308 674 64 EcoRI G'AATT_C Cuts at: 3355 3733 3355 Size: 378 5668 FspI TGC'GCA Cuts at: 4552 4552 Size: 6046 HaeII r_GCGC'y Cuts at: 1678 2084 2469 2930 5193 1678 Size: 406 385 461 2263 2531 Fragments arranged by size: 2531 2263 461 406 385 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4687 4719 4761 4793 1373 Size: 32 135 32 3115 32 42 32 2626 Fragments arranged by size: 3115 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3160 3390 3636 669 Size: 988 281 1222 230 246 3079 Fragments arranged by size: 3079 1222 988 281 246 230 HindIII A'AGCT_T Cuts at: 748 748 Size: 6046 HpaI GTT'AAC Cuts at: 3636 3636 Size: 6046 KasI G'GCGC_C Cuts at: 2080 2080 Size: 6046 KpnI G_GTAC'C Cuts at: 3371 3371 Size: 6046 MfeI C'AATT_G Cuts at: 3645 3645 Size: 6046 MluI A'CGCG_T Cuts at: 5922 5922 Size: 6046 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2191 5160 5344 1305 Size: 886 2969 184 2007 Fragments arranged by size: 2969 2007 886 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2168 2444 10 Size: 54 1425 343 336 276 3612 Fragments arranged by size: 3612 1425 343 336 276 54 MslI CAynn'nnrTG Cuts at: 518 1166 2231 2408 2494 2585 4004 4363 4522 Size: 648 1065 177 86 91 1419 359 159 Cuts at: 4522 518 Size: 2042 Fragments arranged by size: 2042 1419 1065 648 359 177 159 91 86 MspA1I CmG'CkG Cuts at: 1606 2118 3462 4139 5287 5532 1606 Size: 512 1344 677 1148 245 2120 Fragments arranged by size: 2120 1344 1148 677 512 245 NaeI GCC'GGC Cuts at: 2843 2843 Size: 6046 NarI GG'CG_CC Cuts at: 2081 2081 Size: 6046 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4555 Fragments arranged by size: 4555 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6046 NgoMIV G'CCGG_C Cuts at: 2841 2841 Size: 6046 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6046 NotI GC'GGCC_GC Cuts at: 3493 3493 Size: 6046 NruI TCG'CGA Cuts at: 2733 2733 Size: 6046 NspI r_CATG'y Cuts at: 1132 1229 2329 3404 4949 1132 Size: 97 1100 1075 1545 2229 Fragments arranged by size: 2229 1545 1100 1075 97 PasI CC'CwG_GG Cuts at: 1348 3255 1348 Size: 1907 4139 PciI A'CATG_T Cuts at: 4945 4945 Size: 6046 PflMI CCAn_nnn'nTGG Cuts at: 1108 3066 1108 Size: 1958 4088 PmeI GTTT'AAAC Cuts at: 3350 3350 Size: 6046 PmlI CAC'GTG Cuts at: 2538 3066 2538 Size: 528 5518 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4115 4147 5628 5660 4115 Size: 32 1481 32 4501 Fragments arranged by size: 4501 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3274 3274 Size: 6046 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6046 PsiI TTA'TAA Cuts at: 3616 3616 Size: 6046 PspOMI G'GGCC_C Cuts at: 3337 3337 Size: 6046 PstI C_TGCA'G Cuts at: 830 2487 2616 3398 830 Size: 1657 129 782 3478 Fragments arranged by size: 3478 1657 782 129 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6046 PvuII CAG'CTG Cuts at: 2118 2118 Size: 6046 SacI G_AGCT'C Cuts at: 2739 3250 2739 Size: 511 5535 SalI G'TCGA_C Cuts at: 1936 3388 1936 Size: 1452 4594 SapI GCTCTTCn'nnn_ Cuts at: 3267 3267 Size: 6046 SbfI CC_TGCA'GG Cuts at: 3398 3398 Size: 6046 ScaI AGT'ACT Cuts at: 4294 6006 4294 Size: 1712 4334 SexAI A'CCwGG_T Cuts at: 2654 2654 Size: 6046 SfcI C'TryA_G Cuts at: 826 1045 2483 2612 3394 4529 5210 5401 826 Size: 219 1438 129 782 1135 681 191 1471 Fragments arranged by size: 1471 1438 1135 782 681 219 191 129 SfoI GGC'GCC Cuts at: 2082 2082 Size: 6046 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6046 SmaI CCC'GGG Cuts at: 1872 2050 2076 2694 3087 3373 1872 Size: 178 26 618 393 286 4545 Fragments arranged by size: 4545 618 393 286 178 26 SmlI C'TyrA_G Cuts at: 820 1017 1943 4150 5051 5313 820 Size: 197 926 2207 901 262 1553 Fragments arranged by size: 2207 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3837 493 Size: 3344 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6046 SphI G_CATG'C Cuts at: 1132 2329 3404 1132 Size: 1197 1075 3774 Fragments arranged by size: 3774 1197 1075 SspI AAT'ATT Cuts at: 5 52 3970 5 Size: 47 3918 2081 Fragments arranged by size: 3918 2081 47 StuI AGG'CCT Cuts at: 1517 3203 1517 Size: 1686 4360 StyI C'CwwG_G Cuts at: 513 1065 2004 2800 3485 513 Size: 552 939 796 685 3074 Fragments arranged by size: 3074 939 796 685 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2981 4073 4090 4243 4428 4873 325 Size: 1255 1401 1092 17 153 185 445 1498 Fragments arranged by size: 1498 1401 1255 1092 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2577 4292 6004 Size: 274 80 33 51 1443 600 1715 1712 Cuts at: 6004 96 Size: 138 Fragments arranged by size: 1715 1712 1443 600 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6006 6038 6006 Size: 32 6014 Tth111I GACn'n_nGTC Cuts at: 1316 2656 1316 Size: 1340 4706 XbaI T'CTAG_A Cuts at: 3382 3382 Size: 6046 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3331 3331 Size: 6046 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6046 XmaI C'CCGG_G Cuts at: 1870 2048 2074 2692 3085 3371 1870 Size: 178 26 618 393 286 4545 Fragments arranged by size: 4545 618 393 286 178 26 XmnI GAAnn'nnTTC Cuts at: 4175 4175 Size: 6046 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3854 276 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI