(Circular) (Six-base) MAPSORT of: pFN21AE0993.seq Check: 7339 from: 1 to: 6640 pFN21AE0993 6640 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2991 2991 Size: 6640 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4450 278 Size: 53 83 186 936 2914 2468 Fragments arranged by size: 2914 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3983 1937 Size: 2046 4594 Acc65I G'GTAC_C Cuts at: 3961 3961 Size: 6640 AclI AA'CG_TT Cuts at: 1563 4767 5140 1563 Size: 3204 373 3063 Fragments arranged by size: 3204 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3450 3672 4703 6087 6355 1632 Size: 1818 222 1031 1384 268 1917 Fragments arranged by size: 1917 1818 1384 1031 268 222 AflII C'TTAA_G Cuts at: 820 1017 2183 820 Size: 197 1166 5277 Fragments arranged by size: 5277 1166 197 AflIII A'CryG_T Cuts at: 1203 3657 5539 6516 1203 Size: 2454 1882 977 1327 Fragments arranged by size: 2454 1882 1327 977 AgeI A'CCGG_T Cuts at: 5471 5471 Size: 6640 AhdI GACnn_n'nnGTC Cuts at: 2482 2638 2650 5369 2482 Size: 156 12 2719 3753 Fragments arranged by size: 3753 2719 156 12 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6640 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3143 3813 1184 Size: 1959 670 4011 Fragments arranged by size: 4011 1959 670 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3909 3941 3909 Size: 32 6608 AlwNI CAG_nnn'CTG Cuts at: 2431 2992 3160 3739 3855 3877 3898 4383 5955 Size: 561 168 579 116 22 21 485 1572 Cuts at: 5955 2431 Size: 3116 Fragments arranged by size: 3116 1572 579 561 485 168 116 22 21 ApaLI G'TGCA_C Cuts at: 4697 5853 4697 Size: 1156 5484 ApoI r'AATT_y Cuts at: 1493 2012 2361 2844 2923 3949 4175 4327 6436 Size: 519 349 483 79 1026 226 152 2109 Cuts at: 6436 1493 Size: 1697 Fragments arranged by size: 2109 1697 1026 519 483 349 226 152 79 AseI AT'TA_AT Cuts at: 160 1032 2958 5194 160 Size: 872 1926 2236 1606 Fragments arranged by size: 2236 1926 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6640 AvaI C'yCGr_G Cuts at: 1870 1943 3965 1870 Size: 73 2022 4545 Fragments arranged by size: 4545 2022 73 BamHI G'GATC_C Cuts at: 2555 2976 3533 3970 4337 2555 Size: 421 557 437 367 4858 Fragments arranged by size: 4858 557 437 421 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3838 3961 5416 618 Size: 325 217 627 2051 123 1455 1842 Fragments arranged by size: 2051 1842 1455 627 325 217 123 BanII G_rGCy'C Cuts at: 1391 3959 1391 Size: 2568 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2922 3238 3420 3660 928 Size: 971 1023 316 182 240 3908 Fragments arranged by size: 3908 1023 971 316 240 182 BbvCI CC'TCA_GC Cuts at: 3044 3044 Size: 6640 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4831 4865 1903 Size: 34 50 34 2810 34 3678 Fragments arranged by size: 3678 2810 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2457 3389 4532 5742 2457 Size: 932 1143 1210 3355 Fragments arranged by size: 3355 1210 1143 932 BclI T'GATC_A Cuts at: 1548 3900 1548 Size: 2352 4288 BfrBI ATG'CAT Cuts at: 2452 2664 2696 3449 2452 Size: 212 32 753 5643 Fragments arranged by size: 5643 753 212 32 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5251 136 Size: 107 122 71 631 605 3579 1525 Fragments arranged by size: 3579 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2347 3140 6635 2347 Size: 793 3495 2352 Fragments arranged by size: 3495 2352 793 BlpI GC'TnA_GC Cuts at: 4057 4057 Size: 6640 Bme1580I G_kGCm'C Cuts at: 1790 3001 4701 5857 1790 Size: 1211 1700 1156 2573 Fragments arranged by size: 2573 1700 1211 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6640 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4371 5329 442 Size: 416 53 83 399 24 2954 958 1753 Fragments arranged by size: 2954 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3804 1056 Size: 2748 3892 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3038 3911 5300 1239 Size: 1799 873 1389 2579 Fragments arranged by size: 2579 1799 1389 873 Bpu10I CC'TnA_GC Cuts at: 3044 3044 Size: 6640 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3761 3828 4765 5630 5928 3761 Size: 67 937 865 298 4473 Fragments arranged by size: 4473 937 865 298 67 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5303 882 Size: 617 3804 2219 Fragments arranged by size: 3804 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2092 4431 493 Size: 711 888 2339 2702 Fragments arranged by size: 2702 2339 888 711 BsaBI GATnn'nnATC Cuts at: 6634 6634 Size: 6640 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3023 4447 4829 5464 Size: 53 83 186 936 1490 1424 382 635 Cuts at: 5464 275 Size: 1451 Fragments arranged by size: 1490 1451 1424 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5073 5471 5745 5892 6303 5073 Size: 398 274 147 411 5410 Fragments arranged by size: 5410 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2572 2602 2742 2772 3909 Size: 30 165 30 1169 30 140 30 1137 Cuts at: 3909 3939 1178 Size: 30 3879 Fragments arranged by size: 3879 1169 1137 165 140 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2178 2240 2473 3230 3494 1186 Size: 992 62 233 757 264 4332 Fragments arranged by size: 4332 992 757 264 233 62 BseYI C'CCAG_C Cuts at: 2269 2434 3537 3739 5843 6339 2269 Size: 165 1103 202 2104 496 2570 Fragments arranged by size: 2570 2104 1103 496 202 165 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6067 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4090 4851 5879 1540 Size: 275 186 2089 761 1028 2301 Fragments arranged by size: 2301 2089 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4701 4786 5857 4701 Size: 85 1071 5484 Fragments arranged by size: 5484 1071 85 BsmI GAATG_Cn' Cuts at: 2696 3132 3802 4151 4244 2696 Size: 436 670 349 93 5092 Fragments arranged by size: 5092 670 436 349 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3001 3959 4701 4786 5857 1391 Size: 399 1211 958 742 85 1071 2174 Fragments arranged by size: 2174 1211 1071 958 742 399 85 BspEI T'CCGG_A Cuts at: 6303 6303 Size: 6640 BspHI T'CATG_A Cuts at: 4529 6259 4529 Size: 1730 4910 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2991 3324 3995 844 Size: 2147 333 671 3489 Fragments arranged by size: 3489 2147 671 333 BsrBI CCG'CTC Cuts at: 1394 1823 4527 1394 Size: 429 2704 3507 Fragments arranged by size: 3507 2704 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5135 5309 65 Size: 1183 3887 174 1396 Fragments arranged by size: 3887 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5284 5471 1700 Size: 110 3474 187 2869 Fragments arranged by size: 3474 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2224 96 Size: 2128 4512 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6640 BssSI C'ACGA_G Cuts at: 4700 5712 4700 Size: 1012 5628 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6640 BstBI TT'CG_AA Cuts at: 5444 5444 Size: 6640 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6640 BstXI CCAn_nnnn'nTGG Cuts at: 3595 3595 Size: 6640 BstYI r'GATC_y Cuts at: 1972 2347 2555 2976 3140 3405 3533 3813 3970 Size: 375 208 421 164 265 128 280 157 Cuts at: 3970 4337 4722 4739 6191 6277 6289 6635 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 421 385 375 367 346 280 265 208 164 157 128 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2607 3225 2607 Size: 618 6022 BtgI C'CryG_G Cuts at: 513 1065 3342 3645 513 Size: 552 2277 303 3508 Fragments arranged by size: 3508 2277 552 303 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5901 Fragments arranged by size: 5901 683 56 BtsI GCAGTG_nn' Cuts at: 2119 4156 4950 4970 2119 Size: 2037 794 20 3789 Fragments arranged by size: 3789 2037 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6640 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2280 2315 529 Size: 35 1716 35 4854 Fragments arranged by size: 4854 1716 35 35 DraI TTT'AAA Cuts at: 2730 2850 3195 3944 2730 Size: 120 345 749 5426 Fragments arranged by size: 5426 749 345 120 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5647 809 Size: 507 4331 1802 Fragments arranged by size: 4331 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2568 3585 3693 Size: 54 1425 50 275 18 738 1017 108 Cuts at: 3693 4087 4976 6605 8 Size: 394 889 1629 43 Fragments arranged by size: 1629 1425 1017 889 738 394 275 108 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4087 1537 Size: 275 2275 4090 Fragments arranged by size: 4090 2275 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3258 3279 3426 3462 3753 3882 4570 1353 Size: 1905 21 147 36 291 129 688 3423 Fragments arranged by size: 3423 1905 688 291 147 129 36 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5227 5601 5747 387 Size: 1064 138 3638 374 146 1280 Fragments arranged by size: 3638 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3038 3450 3672 3911 4703 5300 6087 Size: 393 1406 412 222 239 792 597 787 Cuts at: 6087 6355 1239 Size: 268 1524 Fragments arranged by size: 1524 1406 792 787 597 412 393 268 239 222 EcoRI G'AATT_C Cuts at: 3949 4327 3949 Size: 378 6262 EcoRV GAT'ATC Cuts at: 2771 3574 2771 Size: 803 5837 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3255 3437 3255 Size: 182 6458 FspI TGC'GCA Cuts at: 5146 5146 Size: 6640 HaeII r_GCGC'y Cuts at: 1678 5787 1678 Size: 4109 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2455 2468 2487 2500 2624 Size: 32 135 32 883 13 19 13 124 Cuts at: 2624 2636 2656 2668 3368 3400 5281 5313 5355 Size: 12 20 12 700 32 1881 32 42 Cuts at: 5355 5387 1373 Size: 32 2626 Fragments arranged by size: 2626 1881 883 700 135 124 42 32 32 32 32 32 20 19 13 13 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 2443 3984 4230 669 Size: 988 281 505 1541 246 3079 Fragments arranged by size: 3079 1541 988 505 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6640 HpaI GTT'AAC Cuts at: 4230 4230 Size: 6640 KpnI G_GTAC'C Cuts at: 3965 3965 Size: 6640 MfeI C'AATT_G Cuts at: 3366 4239 3366 Size: 873 5767 MluI A'CGCG_T Cuts at: 6516 6516 Size: 6640 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2179 2622 5754 5938 1305 Size: 874 443 3132 184 2007 Fragments arranged by size: 3132 2007 874 443 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2570 3587 3695 10 Size: 54 1425 343 738 1017 108 2955 Fragments arranged by size: 2955 1425 1017 738 343 108 54 MslI CAynn'nnrTG Cuts at: 518 1166 2659 3644 3795 4598 4957 5116 518 Size: 648 1493 985 151 803 359 159 2042 Fragments arranged by size: 2042 1493 985 803 648 359 159 151 MspA1I CmG'CkG Cuts at: 1606 2428 3160 4056 4733 5881 6126 1606 Size: 822 732 896 677 1148 245 2120 Fragments arranged by size: 2120 1148 896 822 732 677 245 NcoI C'CATG_G Cuts at: 513 1065 3342 3645 513 Size: 552 2277 303 3508 Fragments arranged by size: 3508 2277 552 303 NdeI CA'TA_TG Cuts at: 387 387 Size: 6640 NheI G'CTAG_C Cuts at: 1052 3800 1052 Size: 2748 3892 NotI GC'GGCC_GC Cuts at: 4087 4087 Size: 6640 NsiI A_TGCA'T Cuts at: 2454 2666 2698 3451 2454 Size: 212 32 753 5643 Fragments arranged by size: 5643 753 212 32 NspI r_CATG'y Cuts at: 1132 1229 2452 2664 2806 3661 3998 5543 1132 Size: 97 1223 212 142 855 337 1545 2229 Fragments arranged by size: 2229 1545 1223 855 337 212 142 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6640 PciI A'CATG_T Cuts at: 3657 5539 3657 Size: 1882 4758 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6640 PmeI GTTT'AAAC Cuts at: 3944 3944 Size: 6640 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3909 3941 4709 4741 6222 6254 3909 Size: 32 768 32 1481 32 4295 Fragments arranged by size: 4295 1481 768 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6640 PsiI TTA'TAA Cuts at: 4210 4210 Size: 6640 PstI C_TGCA'G Cuts at: 830 2988 3992 830 Size: 2158 1004 3478 Fragments arranged by size: 3478 2158 1004 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6640 PvuII CAG'CTG Cuts at: 2428 3160 2428 Size: 732 5908 SalI G'TCGA_C Cuts at: 1936 3982 1936 Size: 2046 4594 SapI GCTCTTCn'nnn_ Cuts at: 3882 3882 Size: 6640 SbfI CC_TGCA'GG Cuts at: 3992 3992 Size: 6640 ScaI AGT'ACT Cuts at: 2333 3437 4888 6600 2333 Size: 1104 1451 1712 2373 Fragments arranged by size: 2373 1712 1451 1104 SfcI C'TryA_G Cuts at: 826 1045 2876 2984 3988 5123 5804 5995 826 Size: 219 1831 108 1004 1135 681 191 1471 Fragments arranged by size: 1831 1471 1135 1004 681 219 191 108 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6640 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6640 SmaI CCC'GGG Cuts at: 1872 3967 1872 Size: 2095 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2183 3776 3843 4744 5645 5907 Size: 197 926 240 1593 67 901 901 262 Cuts at: 5907 820 Size: 1553 Fragments arranged by size: 1593 1553 926 901 901 262 240 197 67 SnaBI TAC'GTA Cuts at: 493 4431 493 Size: 3938 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6640 SphI G_CATG'C Cuts at: 1132 2664 3998 1132 Size: 1532 1334 3774 Fragments arranged by size: 3774 1532 1334 SspI AAT'ATT Cuts at: 5 52 4564 5 Size: 47 4512 2081 Fragments arranged by size: 4512 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6640 StyI C'CwwG_G Cuts at: 513 1065 2623 3342 3391 3645 4079 513 Size: 552 1558 719 49 254 434 3074 Fragments arranged by size: 3074 1558 719 552 434 254 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2283 4667 4684 4837 5022 5467 325 Size: 1255 703 2384 17 153 185 445 1498 Fragments arranged by size: 2384 1498 1255 703 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2224 2331 3010 Size: 274 80 33 51 1443 247 107 679 Cuts at: 3010 3172 3435 3879 4886 6598 96 Size: 162 263 444 1007 1712 138 Fragments arranged by size: 1712 1443 1007 679 444 274 263 247 162 138 107 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2742 2774 6600 6632 2742 Size: 32 3826 32 2750 Fragments arranged by size: 3826 2750 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6640 XbaI T'CTAG_A Cuts at: 3622 3976 3622 Size: 354 6286 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3643 3736 3643 Size: 93 6547 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6640 XmaI C'CCGG_G Cuts at: 1870 3965 1870 Size: 2095 4545 XmnI GAAnn'nnTTC Cuts at: 3235 4769 3235 Size: 1534 5106 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4448 276 Size: 53 83 186 936 2914 2468 Fragments arranged by size: 2914 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI