(Circular) (Six-base) MAPSORT of: pFN21AB0947.seq Check: 5559 from: 1 to: 5239 pFN21AB0947 5239 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3049 278 Size: 53 83 186 936 1513 2468 Fragments arranged by size: 2468 1513 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2291 2582 1937 Size: 354 291 4594 Fragments arranged by size: 4594 354 291 Acc65I G'GTAC_C Cuts at: 2560 2560 Size: 5239 AclI AA'CG_TT Cuts at: 1563 3366 3739 1563 Size: 1803 373 3063 Fragments arranged by size: 3063 1803 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2261 3302 4686 4954 1632 Size: 629 1041 1384 268 1917 Fragments arranged by size: 1917 1384 1041 629 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5042 AflIII A'CryG_T Cuts at: 1203 4138 5115 1203 Size: 2935 977 1327 Fragments arranged by size: 2935 1327 977 AgeI A'CCGG_T Cuts at: 4070 4070 Size: 5239 AhdI GACnn_n'nnGTC Cuts at: 3968 3968 Size: 5239 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5239 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5239 AlwNI CAG_nnn'CTG Cuts at: 2531 2982 4554 2531 Size: 451 1572 3216 Fragments arranged by size: 3216 1572 451 ApaLI G'TGCA_C Cuts at: 3296 4452 3296 Size: 1156 4083 ApoI r'AATT_y Cuts at: 1493 2548 2774 2926 5035 1493 Size: 1055 226 152 2109 1697 Fragments arranged by size: 2109 1697 1055 226 152 AseI AT'TA_AT Cuts at: 160 1032 3793 160 Size: 872 2761 1606 Fragments arranged by size: 2761 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5239 AvaI C'yCGr_G Cuts at: 1870 1943 2564 1870 Size: 73 621 4545 Fragments arranged by size: 4545 621 73 BamHI G'GATC_C Cuts at: 2032 2569 2936 2032 Size: 537 367 4335 Fragments arranged by size: 4335 537 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2560 4015 618 Size: 325 217 627 773 1455 1842 Fragments arranged by size: 1842 1455 773 627 325 217 BanII G_rGCy'C Cuts at: 1391 2558 1391 Size: 1167 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2155 928 Size: 971 256 4012 Fragments arranged by size: 4012 971 256 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3430 3464 1903 Size: 34 50 34 1409 34 3678 Fragments arranged by size: 3678 1409 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3131 4341 3131 Size: 1210 4029 BclI T'GATC_A Cuts at: 1548 1548 Size: 5239 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3850 136 Size: 107 122 71 631 605 337 1841 1525 Fragments arranged by size: 1841 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5234 5234 Size: 5239 BlpI GC'TnA_GC Cuts at: 2656 2656 Size: 5239 Bme1580I G_kGCm'C Cuts at: 1790 3300 4456 1790 Size: 1510 1156 2573 Fragments arranged by size: 2573 1510 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5239 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2970 3928 442 Size: 416 53 83 399 24 1553 958 1753 Fragments arranged by size: 1753 1553 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5239 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2343 3899 1239 Size: 1104 1556 2579 Fragments arranged by size: 2579 1556 1104 Bpu10I CC'TnA_GC Cuts at: 2411 2411 Size: 5239 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3364 4229 4527 3364 Size: 865 298 4076 Fragments arranged by size: 4076 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3902 882 Size: 617 2403 2219 Fragments arranged by size: 2403 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3030 493 Size: 711 1826 2702 Fragments arranged by size: 2702 1826 711 BsaBI GATnn'nnATC Cuts at: 5233 5233 Size: 5239 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3046 3428 4063 275 Size: 53 83 186 936 1513 382 635 1451 Fragments arranged by size: 1513 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3672 4070 4344 4491 4902 3672 Size: 398 274 147 411 4009 Fragments arranged by size: 4009 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5014 Fragments arranged by size: 5014 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5239 BseYI C'CCAG_C Cuts at: 2296 2471 4442 4938 2296 Size: 175 1971 496 2597 Fragments arranged by size: 2597 1971 496 175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4666 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2689 3450 4478 1540 Size: 275 186 688 761 1028 2301 Fragments arranged by size: 2301 1028 761 688 275 186 BsiHKAI G_wGCw'C Cuts at: 3300 3385 4456 3300 Size: 85 1071 4083 Fragments arranged by size: 4083 1071 85 BsmI GAATG_Cn' Cuts at: 2750 2843 2750 Size: 93 5146 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2558 3300 3385 4456 1391 Size: 399 768 742 85 1071 2174 Fragments arranged by size: 2174 1071 768 742 399 85 BspEI T'CCGG_A Cuts at: 4902 4902 Size: 5239 BspHI T'CATG_A Cuts at: 2508 3128 4858 2508 Size: 620 1730 2889 Fragments arranged by size: 2889 1730 620 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2104 2371 2594 844 Size: 1260 267 223 3489 Fragments arranged by size: 3489 1260 267 223 BsrBI CCG'CTC Cuts at: 1394 1823 3126 1394 Size: 429 1303 3507 Fragments arranged by size: 3507 1303 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3734 3908 65 Size: 1183 2486 174 1396 Fragments arranged by size: 2486 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 3883 4070 1700 Size: 110 198 1875 187 2869 Fragments arranged by size: 2869 1875 198 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5239 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5239 BssSI C'ACGA_G Cuts at: 3299 4311 3299 Size: 1012 4227 BstAPI GCAn_nnn'nTGC Cuts at: 794 2354 794 Size: 1560 3679 BstBI TT'CG_AA Cuts at: 4043 4043 Size: 5239 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5239 BstYI r'GATC_y Cuts at: 1972 2032 2569 2936 3321 3338 4790 4876 4888 Size: 60 537 367 385 17 1452 86 12 Cuts at: 4888 5234 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 537 385 367 346 86 60 17 12 BstZ17I GTA'TAC Cuts at: 2292 2292 Size: 5239 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4687 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4500 Fragments arranged by size: 4500 683 56 BtsI GCAGTG_nn' Cuts at: 2755 3549 3569 2755 Size: 794 20 4425 Fragments arranged by size: 4425 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5239 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5204 DraI TTT'AAA Cuts at: 2543 2543 Size: 5239 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4246 809 Size: 507 2930 1802 Fragments arranged by size: 2930 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2686 3575 5204 Size: 54 1425 50 275 18 856 889 1629 Cuts at: 5204 8 Size: 43 Fragments arranged by size: 1629 1425 889 856 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2686 1537 Size: 275 874 4090 Fragments arranged by size: 4090 874 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2026 2492 3169 1353 Size: 673 466 677 3423 Fragments arranged by size: 3423 677 673 466 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3826 4200 4346 387 Size: 1064 138 2237 374 146 1280 Fragments arranged by size: 2237 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 2343 3302 3899 4686 4954 1239 Size: 393 629 82 959 597 787 268 1524 Fragments arranged by size: 1524 959 787 629 597 393 268 82 EcoO109I rG'GnC_Cy Cuts at: 2093 2202 2528 2093 Size: 109 326 4804 Fragments arranged by size: 4804 326 109 EcoRI G'AATT_C Cuts at: 2548 2926 2548 Size: 378 4861 FspI TGC'GCA Cuts at: 3745 3745 Size: 5239 HaeII r_GCGC'y Cuts at: 1678 4386 1678 Size: 2708 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3880 3912 3954 3986 1373 Size: 32 135 32 2308 32 42 32 2626 Fragments arranged by size: 2626 2308 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2583 2829 669 Size: 988 281 645 246 3079 Fragments arranged by size: 3079 988 645 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5239 HpaI GTT'AAC Cuts at: 2829 2829 Size: 5239 KpnI G_GTAC'C Cuts at: 2564 2564 Size: 5239 MfeI C'AATT_G Cuts at: 2838 2838 Size: 5239 MluI A'CGCG_T Cuts at: 5115 5115 Size: 5239 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4353 4537 1305 Size: 3048 184 2007 Fragments arranged by size: 3048 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3417 Fragments arranged by size: 3417 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3197 3556 3715 518 Size: 648 2031 359 159 2042 Fragments arranged by size: 2042 2031 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2655 3332 4480 4725 1606 Size: 1049 677 1148 245 2120 Fragments arranged by size: 2120 1148 1049 677 245 NaeI GCC'GGC Cuts at: 2010 2010 Size: 5239 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4687 NdeI CA'TA_TG Cuts at: 387 387 Size: 5239 NgoMIV G'CCGG_C Cuts at: 2008 2008 Size: 5239 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5239 NotI GC'GGCC_GC Cuts at: 2686 2686 Size: 5239 NspI r_CATG'y Cuts at: 1132 1229 2597 4142 1132 Size: 97 1368 1545 2229 Fragments arranged by size: 2229 1545 1368 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5239 PciI A'CATG_T Cuts at: 4138 4138 Size: 5239 PflMI CCAn_nnn'nTGG Cuts at: 1108 2478 1108 Size: 1370 3869 PfoI T'CCnGG_A Cuts at: 2320 2320 Size: 5239 PmeI GTTT'AAAC Cuts at: 2543 2543 Size: 5239 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3308 3340 4821 4853 3308 Size: 32 1481 32 3694 Fragments arranged by size: 3694 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2093 2202 2528 2093 Size: 109 326 4804 Fragments arranged by size: 4804 326 109 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5239 PsiI TTA'TAA Cuts at: 2809 2809 Size: 5239 PstI C_TGCA'G Cuts at: 830 2591 830 Size: 1761 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5239 SalI G'TCGA_C Cuts at: 1936 2581 1936 Size: 645 4594 SbfI CC_TGCA'GG Cuts at: 2591 2591 Size: 5239 ScaI AGT'ACT Cuts at: 3487 5199 3487 Size: 1712 3527 SfcI C'TryA_G Cuts at: 826 1045 2587 3722 4403 4594 826 Size: 219 1542 1135 681 191 1471 Fragments arranged by size: 1542 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5239 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5239 SmaI CCC'GGG Cuts at: 1872 2566 1872 Size: 694 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3343 4244 4506 820 Size: 197 926 1400 901 262 1553 Fragments arranged by size: 1553 1400 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3030 493 Size: 2537 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5239 SphI G_CATG'C Cuts at: 1132 2597 1132 Size: 1465 3774 SspI AAT'ATT Cuts at: 5 52 3163 5 Size: 47 3111 2081 Fragments arranged by size: 3111 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5239 StyI C'CwwG_G Cuts at: 513 1065 2272 2678 513 Size: 552 1207 406 3074 Fragments arranged by size: 3074 1207 552 406 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3266 3283 3436 3621 4066 325 Size: 1255 1686 17 153 185 445 1498 Fragments arranged by size: 1686 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3485 5197 96 Size: 274 80 33 51 1443 1508 1712 138 Fragments arranged by size: 1712 1508 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2296 2328 5199 5231 2296 Size: 32 2871 32 2304 Fragments arranged by size: 2871 2304 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5239 XbaI T'CTAG_A Cuts at: 2575 2575 Size: 5239 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5239 XmaI C'CCGG_G Cuts at: 1870 2564 1870 Size: 694 4545 XmnI GAAnn'nnTTC Cuts at: 2447 3368 2447 Size: 921 4318 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3047 276 Size: 53 83 186 936 1513 2468 Fragments arranged by size: 2468 1513 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI