Restriction map for
pFN21AB0928
(Circular) (Six-base) MAPSORT of: pFN21AB0928.seq Check: 9400 from: 1 to: 5797
pFN21AB0928 5797 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2276 2276
Size: 5797
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3140 1937
Size: 1203 4594
Acc65I G'GTAC_C
Cuts at: 3118 3118
Size: 5797
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2997 3860 5244 5512 1632
Size: 1365 863 1384 268 1917
Fragments arranged by size:
1917 1384 1365 863 268
AfeI AGC'GCT
Cuts at: 2655 2675 2655
Size: 20 5777
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5600
AflIII A'CryG_T
Cuts at: 1203 2643 4696 5673 1203
Size: 1440 2053 977 1327
Fragments arranged by size:
2053 1440 1327 977
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 4526 4526
Size: 5797
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2441 1184
Size: 1257 4540
AlwNI CAG_nnn'CTG
Cuts at: 2749 2849 2864 2882 3540 5112 2749
Size: 100 15 18 658 1572 3434
Fragments arranged by size:
3434 1572 658 100 18 15
ApaLI G'TGCA_C
Cuts at: 2123 3854 5010 2123
Size: 1731 1156 2910
Fragments arranged by size:
2910 1731 1156
ApoI r'AATT_y
Cuts at: 1493 3028 3106 3332 3484 5593 1493
Size: 1535 78 226 152 2109 1697
Fragments arranged by size:
2109 1697 1535 226 152 78
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2465 3122 1870
Size: 73 91 431 657 4545
Fragments arranged by size:
4545 657 431 91 73
BamHI G'GATC_C
Cuts at: 3127 3494 3127
Size: 367 5430
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3118 4573 618
Size: 325 217 627 1331 1455 1842
Fragments arranged by size:
1842 1455 1331 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2933 3116 1391
Size: 1542 183 4072
Fragments arranged by size:
4072 1542 183
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2139 928
Size: 971 240 4586
Fragments arranged by size:
4586 971 240
BbvCI CC'TCA_GC
Cuts at: 2148 2148
Size: 5797
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2039 2073 3988 4022 1903
Size: 34 102 34 1915 34 3678
Fragments arranged by size:
3678 1915 102 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3077 4408 136
Size: 107 122 71 631 605 1405 1331 1525
Fragments arranged by size:
1525 1405 1331 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 3214 3214
Size: 5797
Bme1580I G_kGCm'C
Cuts at: 1790 2127 2514 3858 5014 1790
Size: 337 387 1344 1156 2573
Fragments arranged by size:
2573 1344 1156 387 337
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5797
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2948 3528 4486
Size: 416 53 83 399 24 1531 580 958
Cuts at: 4486 442
Size: 1753
Fragments arranged by size:
1753 1531 958 580 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4457 1239
Size: 3218 2579
Bpu10I CC'TnA_GC
Cuts at: 2148 2453 2148
Size: 305 5492
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3922 4787 5085 3922
Size: 865 298 4634
Fragments arranged by size:
4634 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2469 4460 882
Size: 617 970 1991 2219
Fragments arranged by size:
2219 1991 970 617
BsaAI yAC'GTr
Cuts at: 493 1204 2431 3588 493
Size: 711 1227 1157 2702
Fragments arranged by size:
2702 1227 1157 711
BsaBI GATnn'nnATC
Cuts at: 5791 5791
Size: 5797
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3604 3986 4621 275
Size: 53 83 186 936 2071 382 635 1451
Fragments arranged by size:
2071 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2311 4230 4628 4902 5049 5460 2311
Size: 1919 398 274 147 411 2648
Fragments arranged by size:
2648 1919 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2783 2813 1178
Size: 30 165 30 1380 30 4162
Fragments arranged by size:
4162 1380 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2315 1186
Size: 1129 4668
BseYI C'CCAG_C
Cuts at: 2754 5000 5496 2754
Size: 2246 496 3055
Fragments arranged by size:
3055 2246 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 2254 2453 1163
Size: 573 417 101 199 4507
Fragments arranged by size:
4507 573 417 199 101
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3076 3247 4008 5036 1540
Size: 275 186 1075 171 761 1028 2301
Fragments arranged by size:
2301 1075 1028 761 275 186 171
BsiHKAI G_wGCw'C
Cuts at: 2127 3858 3943 5014 2127
Size: 1731 85 1071 2910
Fragments arranged by size:
2910 1731 1071 85
BsiWI C'GTAC_G
Cuts at: 2597 2646 2597
Size: 49 5748
BsmI GAATG_Cn'
Cuts at: 2430 3308 3401 2430
Size: 878 93 4826
Fragments arranged by size:
4826 878 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2514 2933 3116 3858 3943 5014
Size: 399 337 387 419 183 742 85 1071
Cuts at: 5014 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 419 399 387 337 183
85
BspEI T'CCGG_A
Cuts at: 5460 5460
Size: 5797
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2037 2175 2276 3152 844
Size: 1193 138 101 876 3489
Fragments arranged by size:
3489 1193 876 138 101
BsrBI CCG'CTC
Cuts at: 1394 1823 2362 2484 3058 3684 1394
Size: 429 539 122 574 626 3507
Fragments arranged by size:
3507 626 574 539 429 122
BsrDI GCAATG_nn'
Cuts at: 65 1248 4292 4466 65
Size: 1183 3044 174 1396
Fragments arranged by size:
3044 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2448 4441 4628 1700
Size: 110 638 1993 187 2869
Fragments arranged by size:
2869 1993 638 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 3857 4869 3857
Size: 1012 4785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2372 2882 794
Size: 1578 510 3709
Fragments arranged by size:
3709 1578 510
BstBI TT'CG_AA
Cuts at: 4601 4601
Size: 5797
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5797
BstYI r'GATC_y
Cuts at: 1972 3127 3494 3879 3896 5348 5434 5446 5792
Size: 1155 367 385 17 1452 86 12 346
Cuts at: 5792 1972
Size: 1977
Fragments arranged by size:
1977 1452 1155 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2842 2842
Size: 5797
BtgI C'CryG_G
Cuts at: 513 1065 2004 2781 513
Size: 552 939 777 3529
Fragments arranged by size:
3529 939 777 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5058
Fragments arranged by size:
5058 683 56
BtsI GCAGTG_nn'
Cuts at: 2382 2598 2709 3313 4107 4127 2382
Size: 216 111 604 794 20 4052
Fragments arranged by size:
4052 794 604 216 111 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4804 809
Size: 507 3488 1802
Fragments arranged by size:
3488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2784 3244 4133
Size: 54 1425 50 275 18 954 460 889
Cuts at: 4133 5762 8
Size: 1629 43
Fragments arranged by size:
1629 1425 954 889 460 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 1537
Size: 275 1432 4090
Fragments arranged by size:
4090 1432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 3727 1353
Size: 719 1655 3423
Fragments arranged by size:
3423 1655 719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4384 4758 4904 387
Size: 1064 138 2795 374 146 1280
Fragments arranged by size:
2795 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2997 3860 4457 5244 5512 1239
Size: 393 1365 863 597 787 268 1524
Fragments arranged by size:
1524 1365 863 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2392 2392
Size: 5797
EcoRI G'AATT_C
Cuts at: 3028 3106 3484 3028
Size: 78 378 5341
Fragments arranged by size:
5341 378 78
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 2657 2677 4944 1678
Size: 979 20 2267 2531
Fragments arranged by size:
2531 2267 979 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4438 4470 4512 4544 1373
Size: 32 135 32 2866 32 42 32 2626
Fragments arranged by size:
2866 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3141 3387 669
Size: 988 281 1203 246 3079
Fragments arranged by size:
3079 1203 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2180 3009 748
Size: 1432 829 3536
Fragments arranged by size:
3536 1432 829
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KpnI G_GTAC'C
Cuts at: 3122 3122
Size: 5797
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 2643 5673 2643
Size: 3030 2767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2947 4911 5095 1305
Size: 1642 1964 184 2007
Fragments arranged by size:
2007 1964 1642 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2786 10
Size: 54 1425 343 954 3021
Fragments arranged by size:
3021 1425 954 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2994 3755 4114 4273 518
Size: 648 1035 793 761 359 159 2042
Fragments arranged by size:
2042 1035 793 761 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2147 2711 2717 2758 2861 2882 3213 3890
Size: 541 564 6 41 103 21 331 677
Cuts at: 3890 5038 5283 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 564 541 331 245 103
41 21 6
NaeI GCC'GGC
Cuts at: 2450 2450
Size: 5797
NcoI C'CATG_G
Cuts at: 513 1065 2004 2781 513
Size: 552 939 777 3529
Fragments arranged by size:
3529 939 777 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5797
NgoMIV G'CCGG_C
Cuts at: 2448 2448
Size: 5797
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 2249 3155 4700 1132
Size: 97 1020 906 1545 2229
Fragments arranged by size:
2229 1545 1020 906 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5797
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5797
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PmlI CAC'GTG
Cuts at: 2431 2431
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 5379 5411 3866
Size: 32 1481 32 4252
Fragments arranged by size:
4252 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PstI C_TGCA'G
Cuts at: 830 2716 2872 2899 3149 830
Size: 1886 156 27 250 3478
Fragments arranged by size:
3478 1886 250 156 27
PvuI CG_AT'CG
Cuts at: 2001 3076 2001
Size: 1075 4722
PvuII CAG'CTG
Cuts at: 2147 2711 2717 2758 2861 2147
Size: 564 6 41 103 5083
Fragments arranged by size:
5083 564 103 41 6
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SfcI C'TryA_G
Cuts at: 826 1045 2043 2712 2868 2895 2946 3145 4280
Size: 219 998 669 156 27 51 199 1135
Cuts at: 4280 4961 5152 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 998 681 669 219 199 191
156 51 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 2036 2467 3124 1872
Size: 164 431 657 4545
Fragments arranged by size:
4545 657 431 164
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3901 4802 5064 820
Size: 197 926 1958 901 262 1553
Fragments arranged by size:
1958 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 3155 1132
Size: 2023 3774
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5797
StyI C'CwwG_G
Cuts at: 513 1065 2004 2781 3236 513
Size: 552 939 777 455 3074
Fragments arranged by size:
3074 939 777 552 455
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2579 3824 3841 3994 4179 4624 325
Size: 1255 999 1245 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1245 999 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4043 5755 96
Size: 274 80 33 51 1443 2066 1712 138
Fragments arranged by size:
2066 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2783 2815 5757 5789 2783
Size: 32 2942 32 2791
Fragments arranged by size:
2942 2791 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2749 1316
Size: 1433 4364
XbaI T'CTAG_A
Cuts at: 3133 3133
Size: 5797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 2034 2465 3122 1870
Size: 164 431 657 4545
Fragments arranged by size:
4545 657 431 164
XmnI GAAnn'nnTTC
Cuts at: 2015 3926 2015
Size: 1911 3886
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsmBI
BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI