Restriction map for
pFN21AB0928
(Linear) (Six-base) MAPSORT of: KIBB0928.seq Check: 8750 from: 1 to: 1092
KIBB0928.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 271 1092
Size: 271 821
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 992 1092
Size: 992 100
AfeI AGC'GCT
Cuts at: 0 650 670 1092
Size: 650 20 422
Fragments arranged by size:
650 422 20
AflIII A'CryG_T
Cuts at: 0 638 1092
Size: 638 454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 436 1092
Size: 436 656
AlwNI CAG_nnn'CTG
Cuts at: 0 744 844 859 877 1092
Size: 744 100 15 18 215
Fragments arranged by size:
744 215 100 18 15
ApaLI G'TGCA_C
Cuts at: 0 118 1092
Size: 118 974
ApoI r'AATT_y
Cuts at: 0 1023 1092
Size: 1023 69
AvaI C'yCGr_G
Cuts at: 0 29 460 1092
Size: 29 431 632
Fragments arranged by size:
632 431 29
BanII G_rGCy'C
Cuts at: 0 928 1092
Size: 928 164
BbsI GAAGACnn'nnnn_
Cuts at: 0 134 1092
Size: 134 958
BbvCI CC'TCA_GC
Cuts at: 0 143 1092
Size: 143 949
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 68 1092
Size: 34 34 1024
Fragments arranged by size:
1024 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1072 1092
Size: 1072 20
Bme1580I G_kGCm'C
Cuts at: 0 122 509 1092
Size: 122 387 583
Fragments arranged by size:
583 387 122
BmrI ACTGGGnnnn_n'
Cuts at: 0 943 1092
Size: 943 149
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 72 1092
Size: 72 1020
Bpu10I CC'TnA_GC
Cuts at: 0 143 448 1092
Size: 143 305 644
Fragments arranged by size:
644 305 143
BsaI GGTCTCn'nnnn_
Cuts at: 0 464 1092
Size: 464 628
BsaAI yAC'GTr
Cuts at: 0 426 1092
Size: 426 666
BsaWI w'CCGG_w
Cuts at: 0 306 1092
Size: 306 786
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 778 808 1092
Size: 778 30 284
Fragments arranged by size:
778 284 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 310 1092
Size: 310 782
BseYI C'CCAG_C
Cuts at: 0 749 1092
Size: 749 343
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 249 448 1092
Size: 148 101 199 644
Fragments arranged by size:
644 199 148 101
BsiEI CG_ry'CG
Cuts at: 0 1071 1092
Size: 1071 21
BsiHKAI G_wGCw'C
Cuts at: 0 122 1092
Size: 122 970
BsiWI C'GTAC_G
Cuts at: 0 592 641 1092
Size: 592 49 451
Fragments arranged by size:
592 451 49
BsmI GAATG_Cn'
Cuts at: 0 425 1092
Size: 425 667
Bsp1286I G_dGCh'C
Cuts at: 0 122 509 928 1092
Size: 122 387 419 164
Fragments arranged by size:
419 387 164 122
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 32 170 271 1092
Size: 32 138 101 821
Fragments arranged by size:
821 138 101 32
BsrBI CCG'CTC
Cuts at: 0 357 479 1053 1092
Size: 357 122 574 39
Fragments arranged by size:
574 357 122 39
BsrFI r'CCGG_y
Cuts at: 0 443 1092
Size: 443 649
BstAPI GCAn_nnn'nTGC
Cuts at: 0 367 877 1092
Size: 367 510 215
Fragments arranged by size:
510 367 215
Bsu36I CC'TnA_GG
Cuts at: 0 837 1092
Size: 837 255
BtgI C'CryG_G
Cuts at: 0 776 1092
Size: 776 316
BtsI GCAGTG_nn'
Cuts at: 0 377 593 704 1092
Size: 377 216 111 388
Fragments arranged by size:
388 377 216 111
EaeI y'GGCC_r
Cuts at: 0 779 1092
Size: 779 313
EarI CTCTTCn'nnn_
Cuts at: 0 67 1092
Size: 67 1025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 992 1092
Size: 992 100
EcoO109I rG'GnC_Cy
Cuts at: 0 387 1092
Size: 387 705
EcoRI G'AATT_C
Cuts at: 0 1023 1092
Size: 1023 69
HaeII r_GCGC'y
Cuts at: 0 652 672 1092
Size: 652 20 420
Fragments arranged by size:
652 420 20
HindIII A'AGCT_T
Cuts at: 0 175 1004 1092
Size: 175 829 88
Fragments arranged by size:
829 175 88
MluI A'CGCG_T
Cuts at: 0 638 1092
Size: 638 454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 942 1092
Size: 942 150
MscI TGG'CCA
Cuts at: 0 781 1092
Size: 781 311
MslI CAynn'nnrTG
Cuts at: 0 196 989 1092
Size: 196 793 103
Fragments arranged by size:
793 196 103
MspA1I CmG'CkG
Cuts at: 0 142 706 712 753 856 877 1092
Size: 142 564 6 41 103 21 215
Fragments arranged by size:
564 215 142 103 41 21 6
NaeI GCC'GGC
Cuts at: 0 445 1092
Size: 445 647
NcoI C'CATG_G
Cuts at: 0 776 1092
Size: 776 316
NgoMIV G'CCGG_C
Cuts at: 0 443 1092
Size: 443 649
NspI r_CATG'y
Cuts at: 0 244 1092
Size: 244 848
PmlI CAC'GTG
Cuts at: 0 426 1092
Size: 426 666
PstI C_TGCA'G
Cuts at: 0 711 867 894 1092
Size: 711 156 27 198
Fragments arranged by size:
711 198 156 27
PvuI CG_AT'CG
Cuts at: 0 1071 1092
Size: 1071 21
PvuII CAG'CTG
Cuts at: 0 142 706 712 753 856 1092
Size: 142 564 6 41 103 236
Fragments arranged by size:
564 236 142 103 41 6
SfcI C'TryA_G
Cuts at: 0 38 707 863 890 941 1092
Size: 38 669 156 27 51 151
Fragments arranged by size:
669 156 151 51 38 27
SmaI CCC'GGG
Cuts at: 0 31 462 1092
Size: 31 431 630
Fragments arranged by size:
630 431 31
StyI C'CwwG_G
Cuts at: 0 776 1092
Size: 776 316
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 574 1092
Size: 574 518
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 778 810 1092
Size: 778 32 282
Fragments arranged by size:
778 282 32
Tth111I GACn'n_nGTC
Cuts at: 0 744 1092
Size: 744 348
XmaI C'CCGG_G
Cuts at: 0 29 460 1092
Size: 29 431 632
Fragments arranged by size:
632 431 29
XmnI GAAnn'nnTTC
Cuts at: 0 10 1092
Size: 10 1082
Enzymes that do cut:
AarI AcuI AfeI AflIII AlfI AlwNI ApaLI ApoI
AvaI BanII BbsI BbvCI BcgI BglI Bme1580I BmrI
BplI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspMI BsrBI
BsrFI BstAPI Bsu36I BtgI BtsI EaeI EarI Eco57MI
EcoO109I EcoRI HaeII HindIII MluI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PmlI PstI PvuI
PvuII SfcI SmaI StyI TaqII TstI Tth111I XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BpmI BpuEI BsaBI BsaHI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
Hin4I HincII HpaI KasI KpnI MfeI NarI NdeI
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI XbaI
XcmI XhoI ZraI