(Circular) (Six-base) MAPSORT of: pFN21AB9995.seq Check: 4259 from: 1 to: 6460 pFN21AB9995 6460 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4270 278 Size: 53 83 186 936 2734 2468 Fragments arranged by size: 2734 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3803 1937 Size: 1866 4594 Acc65I G'GTAC_C Cuts at: 3781 3781 Size: 6460 AclI AA'CG_TT Cuts at: 1563 4587 4960 1563 Size: 3024 373 3063 Fragments arranged by size: 3063 3024 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2345 4523 5907 6175 1632 Size: 713 2178 1384 268 1917 Fragments arranged by size: 2178 1917 1384 713 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6263 AflIII A'CryG_T Cuts at: 1203 2271 2613 2745 5359 6336 1203 Size: 1068 342 132 2614 977 1327 Fragments arranged by size: 2614 1327 1068 977 342 132 AgeI A'CCGG_T Cuts at: 5291 5291 Size: 6460 AhdI GACnn_n'nnGTC Cuts at: 5189 5189 Size: 6460 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6460 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3111 3234 3281 1184 Size: 1927 123 47 4363 Fragments arranged by size: 4363 1927 123 47 AlwNI CAG_nnn'CTG Cuts at: 4203 5775 4203 Size: 1572 4888 ApaLI G'TGCA_C Cuts at: 4517 5673 4517 Size: 1156 5304 ApoI r'AATT_y Cuts at: 1493 2467 2478 3769 3995 4147 6256 1493 Size: 974 11 1291 226 152 2109 1697 Fragments arranged by size: 2109 1697 1291 974 226 152 11 AseI AT'TA_AT Cuts at: 160 1032 5014 160 Size: 872 3982 1606 Fragments arranged by size: 3982 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6460 AvaI C'yCGr_G Cuts at: 1870 1943 3785 1870 Size: 73 1842 4545 Fragments arranged by size: 4545 1842 73 BamHI G'GATC_C Cuts at: 3790 4157 3790 Size: 367 6093 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2091 2732 3781 5236 618 Size: 325 217 627 304 641 1049 1455 1842 Fragments arranged by size: 1842 1455 1049 641 627 325 304 217 BanII G_rGCy'C Cuts at: 1391 2021 2705 3779 1391 Size: 630 684 1074 4072 Fragments arranged by size: 4072 1074 684 630 BbeI G_GCGC'C Cuts at: 2736 2736 Size: 6460 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3561 928 Size: 971 1662 3827 Fragments arranged by size: 3827 1662 971 BbvCI CC'TCA_GC Cuts at: 3501 3501 Size: 6460 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2073 2107 4651 4685 1903 Size: 34 136 34 2544 34 3678 Fragments arranged by size: 3678 2544 136 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4352 5562 4352 Size: 1210 5250 BclI T'GATC_A Cuts at: 1548 1548 Size: 6460 BfrBI ATG'CAT Cuts at: 2488 3088 3268 2488 Size: 600 180 5680 Fragments arranged by size: 5680 600 180 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2213 5071 136 Size: 107 122 71 631 605 541 2858 1525 Fragments arranged by size: 2858 1525 631 605 541 122 107 71 BglII A'GATC_T Cuts at: 3078 6455 3078 Size: 3377 3083 BlpI GC'TnA_GC Cuts at: 3877 3877 Size: 6460 Bme1580I G_kGCm'C Cuts at: 1790 4521 5677 1790 Size: 2731 1156 2573 Fragments arranged by size: 2731 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2835 3177 1318 Size: 1517 342 4601 Fragments arranged by size: 4601 1517 342 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4191 5149 442 Size: 416 53 83 399 24 2774 958 1753 Fragments arranged by size: 2774 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6460 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2619 3342 3579 5120 1239 Size: 1380 723 237 1541 2579 Fragments arranged by size: 2579 1541 1380 723 237 Bpu10I CC'TnA_GC Cuts at: 3501 3501 Size: 6460 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2843 4585 5450 5748 2843 Size: 1742 865 298 3555 Fragments arranged by size: 3555 1742 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5123 882 Size: 617 3624 2219 Fragments arranged by size: 3624 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4251 493 Size: 711 3047 2702 Fragments arranged by size: 3047 2702 711 BsaBI GATnn'nnATC Cuts at: 6454 6454 Size: 6460 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2157 2733 3346 4267 Size: 53 83 186 936 624 576 613 921 Cuts at: 4267 4649 5284 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 921 635 624 613 576 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4893 5291 5565 5712 6123 4893 Size: 398 274 147 411 5230 Fragments arranged by size: 5230 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3648 3678 1178 Size: 30 165 30 2245 30 3960 Fragments arranged by size: 3960 2245 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6460 BseYI C'CCAG_C Cuts at: 3694 5663 6159 3694 Size: 1969 496 3995 Fragments arranged by size: 3995 1969 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5887 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3910 4671 5699 1540 Size: 275 186 1909 761 1028 2301 Fragments arranged by size: 2301 1909 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3218 4521 4606 5677 3218 Size: 1303 85 1071 4001 Fragments arranged by size: 4001 1303 1071 85 BsmI GAATG_Cn' Cuts at: 3971 4064 3971 Size: 93 6367 BsmBI CGTCTCn'nnnn_ Cuts at: 2148 2148 Size: 6460 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2705 3218 3779 4521 4606 5677 Size: 399 231 684 513 561 742 85 1071 Cuts at: 5677 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 684 561 513 399 231 85 BspEI T'CCGG_A Cuts at: 6123 6123 Size: 6460 BspHI T'CATG_A Cuts at: 4349 6079 4349 Size: 1730 4730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2301 3815 844 Size: 1457 1514 3489 Fragments arranged by size: 3489 1514 1457 BsrBI CCG'CTC Cuts at: 1394 1823 2178 4347 1394 Size: 429 355 2169 3507 Fragments arranged by size: 3507 2169 429 355 BsrDI GCAATG_nn' Cuts at: 65 1248 2488 2823 4955 5129 65 Size: 1183 1240 335 2132 174 1396 Fragments arranged by size: 2132 1396 1240 1183 335 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2167 2299 5104 5291 1700 Size: 110 357 132 2805 187 2869 Fragments arranged by size: 2869 2805 357 187 132 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6460 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6460 BssSI C'ACGA_G Cuts at: 2996 4520 5532 2996 Size: 1524 1012 3924 Fragments arranged by size: 3924 1524 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6460 BstBI TT'CG_AA Cuts at: 5264 5264 Size: 6460 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6460 BstXI CCAn_nnnn'nTGG Cuts at: 3616 3616 Size: 6460 BstYI r'GATC_y Cuts at: 1972 3078 3790 4157 4542 4559 6011 6097 6109 Size: 1106 712 367 385 17 1452 86 12 Cuts at: 6109 6455 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1106 712 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2878 2878 Size: 6460 BtgI C'CryG_G Cuts at: 513 1065 2004 2195 513 Size: 552 939 191 4778 Fragments arranged by size: 4778 939 552 191 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2724 487 Size: 683 56 1498 4223 Fragments arranged by size: 4223 1498 683 56 BtsI GCAGTG_nn' Cuts at: 3218 3976 4770 4790 3218 Size: 758 794 20 4888 Fragments arranged by size: 4888 794 758 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6460 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2451 2486 529 Size: 35 1887 35 4503 Fragments arranged by size: 4503 1887 35 35 DraI TTT'AAA Cuts at: 3364 3764 3364 Size: 400 6060 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5467 809 Size: 507 4151 1802 Fragments arranged by size: 4151 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2693 3907 4796 Size: 54 1425 50 275 18 863 1214 889 Cuts at: 4796 6425 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1214 889 863 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3907 1537 Size: 275 2095 4090 Fragments arranged by size: 4090 2095 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2817 3231 3558 4390 1353 Size: 1464 414 327 832 3423 Fragments arranged by size: 3423 1464 832 414 327 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2186 5047 5421 5567 387 Size: 1064 138 597 2861 374 146 1280 Fragments arranged by size: 2861 1280 1064 597 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2619 3342 3579 4523 5120 5907 Size: 393 713 274 723 237 944 597 787 Cuts at: 5907 6175 1239 Size: 268 1524 Fragments arranged by size: 1524 944 787 723 713 597 393 274 268 237 EcoNI CCTnn'n_nnAGG Cuts at: 3584 3584 Size: 6460 EcoRI G'AATT_C Cuts at: 3769 4147 3769 Size: 378 6082 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2340 2340 Size: 6460 FspI TGC'GCA Cuts at: 4966 4966 Size: 6460 HaeII r_GCGC'y Cuts at: 1678 2736 5607 1678 Size: 1058 2871 2531 Fragments arranged by size: 2871 2531 1058 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2707 2739 3166 3198 3559 Size: 32 135 32 1135 32 427 32 361 Cuts at: 3559 3591 3648 3680 5101 5133 5175 5207 1373 Size: 32 57 32 1421 32 42 32 2626 Fragments arranged by size: 2626 1421 1135 427 361 135 57 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2266 3804 4050 669 Size: 988 281 328 1538 246 3079 Fragments arranged by size: 3079 1538 988 328 281 246 HindIII A'AGCT_T Cuts at: 748 3538 748 Size: 2790 3670 HpaI GTT'AAC Cuts at: 4050 4050 Size: 6460 KasI G'GCGC_C Cuts at: 2732 2732 Size: 6460 KpnI G_GTAC'C Cuts at: 3785 3785 Size: 6460 MfeI C'AATT_G Cuts at: 4059 4059 Size: 6460 MluI A'CGCG_T Cuts at: 2271 6336 2271 Size: 4065 2395 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2022 2551 5574 5758 1305 Size: 717 529 3023 184 2007 Fragments arranged by size: 3023 2007 717 529 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2695 10 Size: 54 1425 343 863 3775 Fragments arranged by size: 3775 1425 863 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3093 3354 3510 4418 4777 4936 518 Size: 648 1927 261 156 908 359 159 2042 Fragments arranged by size: 2042 1927 908 648 359 261 159 156 MspA1I CmG'CkG Cuts at: 1606 2197 2247 3011 3698 3876 4553 5701 5946 Size: 591 50 764 687 178 677 1148 245 Cuts at: 5946 1606 Size: 2120 Fragments arranged by size: 2120 1148 764 687 677 591 245 178 50 NaeI GCC'GGC Cuts at: 2169 2301 2169 Size: 132 6328 NarI GG'CG_CC Cuts at: 2733 2733 Size: 6460 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4969 Fragments arranged by size: 4969 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6460 NgoMIV G'CCGG_C Cuts at: 2167 2299 2167 Size: 132 6328 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6460 NotI GC'GGCC_GC Cuts at: 3907 3907 Size: 6460 NsiI A_TGCA'T Cuts at: 2490 3090 3270 2490 Size: 600 180 5680 Fragments arranged by size: 5680 600 180 NspI r_CATG'y Cuts at: 1132 1229 2617 2749 3092 3272 3416 3818 5363 Size: 97 1388 132 343 180 144 402 1545 Cuts at: 5363 1132 Size: 2229 Fragments arranged by size: 2229 1545 1388 402 343 180 144 132 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6460 PciI A'CATG_T Cuts at: 2613 2745 5359 2613 Size: 132 2614 3714 Fragments arranged by size: 3714 2614 132 PflMI CCAn_nnn'nTGG Cuts at: 1108 3652 1108 Size: 2544 3916 PmeI GTTT'AAAC Cuts at: 3764 3764 Size: 6460 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4529 4561 6042 6074 4529 Size: 32 1481 32 4915 Fragments arranged by size: 4915 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6460 PsiI TTA'TAA Cuts at: 4030 4030 Size: 6460 PstI C_TGCA'G Cuts at: 830 2246 2875 3812 830 Size: 1416 629 937 3478 Fragments arranged by size: 3478 1416 937 629 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6460 PvuII CAG'CTG Cuts at: 2247 3011 2247 Size: 764 5696 SacII CC_GC'GG Cuts at: 2198 2198 Size: 6460 SalI G'TCGA_C Cuts at: 1936 3802 1936 Size: 1866 4594 SapI GCTCTTCn'nnn_ Cuts at: 2817 2817 Size: 6460 SbfI CC_TGCA'GG Cuts at: 3812 3812 Size: 6460 ScaI AGT'ACT Cuts at: 4708 6420 4708 Size: 1712 4748 SfcI C'TryA_G Cuts at: 826 1045 2242 2871 3808 4943 5624 5815 826 Size: 219 1197 629 937 1135 681 191 1471 Fragments arranged by size: 1471 1197 1135 937 681 629 219 191 SfoI GGC'GCC Cuts at: 2734 2734 Size: 6460 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6460 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6460 SmaI CCC'GGG Cuts at: 1872 3787 1872 Size: 1915 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2858 4564 5465 5727 820 Size: 197 926 915 1706 901 262 1553 Fragments arranged by size: 1706 1553 926 915 901 262 197 SnaBI TAC'GTA Cuts at: 493 4251 493 Size: 3758 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6460 SphI G_CATG'C Cuts at: 1132 3272 3818 1132 Size: 2140 546 3774 Fragments arranged by size: 3774 2140 546 SspI AAT'ATT Cuts at: 5 52 4384 5 Size: 47 4332 2081 Fragments arranged by size: 4332 2081 47 StuI AGG'CCT Cuts at: 1517 3332 1517 Size: 1815 4645 StyI C'CwwG_G Cuts at: 513 1065 2004 3651 3745 3899 513 Size: 552 939 1647 94 154 3074 Fragments arranged by size: 3074 1647 939 552 154 94 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4487 4504 4657 4842 5287 325 Size: 1255 2907 17 153 185 445 1498 Fragments arranged by size: 2907 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4706 6418 96 Size: 274 80 33 51 1443 2729 1712 138 Fragments arranged by size: 2729 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3006 3038 3133 3165 6420 6452 3006 Size: 32 95 32 3255 32 3014 Fragments arranged by size: 3255 3014 95 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3662 1316 Size: 2346 4114 XbaI T'CTAG_A Cuts at: 3796 3796 Size: 6460 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6460 XmaI C'CCGG_G Cuts at: 1870 3785 1870 Size: 1915 4545 XmnI GAAnn'nnTTC Cuts at: 2378 4589 2378 Size: 2211 4249 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4268 276 Size: 53 83 186 936 2734 2468 Fragments arranged by size: 2734 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BplI BsiWI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI XcmI