(Circular) (Six-base) MAPSORT of: pFN21AB7648.seq Check: 8416 from: 1 to: 5881 pFN21AB7648 5881 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3691 278 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3224 1937 Size: 1287 4594 Acc65I G'GTAC_C Cuts at: 3137 3202 3137 Size: 65 5816 AclI AA'CG_TT Cuts at: 1563 4008 4381 1563 Size: 2445 373 3063 Fragments arranged by size: 3063 2445 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 3944 5328 5596 1632 Size: 464 1848 1384 268 1917 Fragments arranged by size: 1917 1848 1384 464 268 AfeI AGC'GCT Cuts at: 2348 2348 Size: 5881 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5684 AflIII A'CryG_T Cuts at: 1203 3028 4780 5757 1203 Size: 1825 1752 977 1327 Fragments arranged by size: 1825 1752 1327 977 AgeI A'CCGG_T Cuts at: 4712 4712 Size: 5881 AhdI GACnn_n'nnGTC Cuts at: 4610 4610 Size: 5881 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5881 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5881 AlwNI CAG_nnn'CTG Cuts at: 3624 5196 3624 Size: 1572 4309 ApaLI G'TGCA_C Cuts at: 3938 5094 3938 Size: 1156 4725 ApoI r'AATT_y Cuts at: 1493 3190 3416 3568 5677 1493 Size: 1697 226 152 2109 1697 Fragments arranged by size: 2109 1697 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4435 160 Size: 872 3403 1606 Fragments arranged by size: 3403 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 AvaI C'yCGr_G Cuts at: 1870 1943 2688 3206 1870 Size: 73 745 518 4545 Fragments arranged by size: 4545 745 518 73 BamHI G'GATC_C Cuts at: 3211 3578 3211 Size: 367 5514 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3137 3202 4657 618 Size: 325 217 627 1350 65 1455 1842 Fragments arranged by size: 1842 1455 1350 627 325 217 65 BanII G_rGCy'C Cuts at: 1391 3200 1391 Size: 1809 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2254 2705 928 Size: 971 355 451 4104 Fragments arranged by size: 4104 971 451 355 BbvCI CC'TCA_GC Cuts at: 2109 2109 Size: 5881 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4072 4106 1903 Size: 34 2135 34 3678 Fragments arranged by size: 3678 2135 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3773 4983 3773 Size: 1210 4671 BclI T'GATC_A Cuts at: 1548 1548 Size: 5881 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2753 4492 136 Size: 107 122 71 631 605 1081 1739 1525 Fragments arranged by size: 1739 1525 1081 631 605 122 107 71 BglII A'GATC_T Cuts at: 5876 5876 Size: 5881 BlpI GC'TnA_GC Cuts at: 3298 3298 Size: 5881 Bme1580I G_kGCm'C Cuts at: 1790 3942 5098 1790 Size: 2152 1156 2573 Fragments arranged by size: 2573 2152 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5881 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2335 2840 3612 Size: 416 53 83 399 24 918 505 772 Cuts at: 3612 4570 442 Size: 958 1753 Fragments arranged by size: 1753 958 918 772 505 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5881 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3082 3097 3082 Size: 15 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2576 2759 4541 1239 Size: 1337 183 1782 2579 Fragments arranged by size: 2579 1782 1337 183 Bpu10I CC'TnA_GC Cuts at: 2109 2109 Size: 5881 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4006 4871 5169 4006 Size: 865 298 4718 Fragments arranged by size: 4718 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4544 882 Size: 617 3045 2219 Fragments arranged by size: 3045 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3672 493 Size: 711 2468 2702 Fragments arranged by size: 2702 2468 711 BsaBI GATnn'nnATC Cuts at: 5875 5875 Size: 5881 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3688 4070 4705 275 Size: 53 83 186 936 2155 382 635 1451 Fragments arranged by size: 2155 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4314 4712 4986 5133 5544 4314 Size: 398 274 147 411 4651 Fragments arranged by size: 4651 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2549 2579 1178 Size: 30 165 30 1146 30 4480 Fragments arranged by size: 4480 1146 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 3089 1186 Size: 916 987 3978 Fragments arranged by size: 3978 987 916 BseYI C'CCAG_C Cuts at: 2877 3008 5084 5580 2877 Size: 131 2076 496 3178 Fragments arranged by size: 3178 2076 496 131 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2618 1163 Size: 573 882 4426 Fragments arranged by size: 4426 882 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3331 4092 5120 1540 Size: 275 186 1330 761 1028 2301 Fragments arranged by size: 2301 1330 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3942 4027 5098 3942 Size: 85 1071 4725 Fragments arranged by size: 4725 1071 85 BsmI GAATG_Cn' Cuts at: 2340 3392 3485 2340 Size: 1052 93 4736 Fragments arranged by size: 4736 1052 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3200 3942 4027 5098 1391 Size: 399 1410 742 85 1071 2174 Fragments arranged by size: 2174 1410 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5544 5544 Size: 5881 BspHI T'CATG_A Cuts at: 2716 3770 5500 2716 Size: 1054 1730 3097 Fragments arranged by size: 3097 1730 1054 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3236 844 Size: 2392 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3768 1394 Size: 429 1945 3507 Fragments arranged by size: 3507 1945 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2494 4376 4550 65 Size: 1183 1246 1882 174 1396 Fragments arranged by size: 1882 1396 1246 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4525 4712 1700 Size: 110 2715 187 2869 Fragments arranged by size: 2869 2715 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5881 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5881 BssSI C'ACGA_G Cuts at: 3941 4953 3941 Size: 1012 4869 BstAPI GCAn_nnn'nTGC Cuts at: 794 2762 794 Size: 1968 3913 BstBI TT'CG_AA Cuts at: 2335 4685 2335 Size: 2350 3531 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5881 BstXI CCAn_nnnn'nTGG Cuts at: 2320 2830 2320 Size: 510 5371 BstYI r'GATC_y Cuts at: 1972 2013 3211 3578 3963 3980 5432 5518 5530 Size: 41 1198 367 385 17 1452 86 12 Cuts at: 5530 5876 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1198 385 367 346 86 41 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2808 3171 513 Size: 552 939 804 363 3223 Fragments arranged by size: 3223 939 804 552 363 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5142 Fragments arranged by size: 5142 683 56 BtsI GCAGTG_nn' Cuts at: 2265 3108 3397 4191 4211 2265 Size: 843 289 794 20 3935 Fragments arranged by size: 3935 843 794 289 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5881 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2851 2886 529 Size: 35 2287 35 3524 Fragments arranged by size: 3524 2287 35 35 DraI TTT'AAA Cuts at: 3185 3185 Size: 5881 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4888 809 Size: 507 3572 1802 Fragments arranged by size: 3572 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3328 4217 5846 Size: 54 1425 50 275 18 1498 889 1629 Cuts at: 5846 8 Size: 43 Fragments arranged by size: 1629 1498 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3328 1537 Size: 275 1516 4090 Fragments arranged by size: 4090 1516 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2739 3811 1353 Size: 1386 1072 3423 Fragments arranged by size: 3423 1386 1072 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2084 2226 4468 4842 4988 387 Size: 1064 138 495 142 2242 374 146 1280 Fragments arranged by size: 2242 1280 1064 495 374 146 142 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2576 2759 3944 4541 5328 5596 Size: 393 464 480 183 1185 597 787 268 Cuts at: 5596 1239 Size: 1524 Fragments arranged by size: 1524 1185 787 597 480 464 393 268 183 EcoO109I rG'GnC_Cy Cuts at: 2113 2113 Size: 5881 EcoRI G'AATT_C Cuts at: 3190 3568 3190 Size: 378 5503 EcoRV GAT'ATC Cuts at: 2602 2602 Size: 5881 FspI TGC'GCA Cuts at: 4387 4387 Size: 5881 HaeII r_GCGC'y Cuts at: 1678 2350 5028 1678 Size: 672 2678 2531 Fragments arranged by size: 2678 2531 672 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2572 2604 3044 3076 4522 Size: 32 135 32 1000 32 440 32 1446 Cuts at: 4522 4554 4596 4628 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1446 1000 440 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2677 3225 3471 669 Size: 988 281 739 548 246 3079 Fragments arranged by size: 3079 988 739 548 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5881 HpaI GTT'AAC Cuts at: 3471 3471 Size: 5881 KpnI G_GTAC'C Cuts at: 3141 3206 3141 Size: 65 5816 MfeI C'AATT_G Cuts at: 3480 3480 Size: 5881 MluI A'CGCG_T Cuts at: 5757 5757 Size: 5881 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2869 4995 5179 1305 Size: 1564 2126 184 2007 Fragments arranged by size: 2126 2007 1564 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4059 Fragments arranged by size: 4059 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2286 3839 4198 4357 518 Size: 648 1120 1553 359 159 2042 Fragments arranged by size: 2042 1553 1120 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2726 3297 3974 5122 5367 1606 Size: 1120 571 677 1148 245 2120 Fragments arranged by size: 2120 1148 1120 677 571 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3171 513 Size: 552 939 1167 3223 Fragments arranged by size: 3223 1167 939 552 NdeI CA'TA_TG Cuts at: 387 2671 387 Size: 2284 3597 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5881 NotI GC'GGCC_GC Cuts at: 3328 3328 Size: 5881 NspI r_CATG'y Cuts at: 1132 1229 2413 3032 3239 4784 1132 Size: 97 1184 619 207 1545 2229 Fragments arranged by size: 2229 1545 1184 619 207 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5881 PciI A'CATG_T Cuts at: 3028 4780 3028 Size: 1752 4129 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5881 PfoI T'CCnGG_A Cuts at: 2553 3091 2553 Size: 538 5343 PmeI GTTT'AAAC Cuts at: 3185 3185 Size: 5881 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3950 3982 5463 5495 3950 Size: 32 1481 32 4336 Fragments arranged by size: 4336 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2113 2113 Size: 5881 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5881 PsiI TTA'TAA Cuts at: 3451 3451 Size: 5881 PstI C_TGCA'G Cuts at: 830 3233 830 Size: 2403 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5881 PvuII CAG'CTG Cuts at: 2726 2726 Size: 5881 SalI G'TCGA_C Cuts at: 1936 3223 1936 Size: 1287 4594 SapI GCTCTTCn'nnn_ Cuts at: 2739 2739 Size: 5881 SbfI CC_TGCA'GG Cuts at: 3233 3233 Size: 5881 ScaI AGT'ACT Cuts at: 4129 5841 4129 Size: 1712 4169 SfcI C'TryA_G Cuts at: 826 1045 3229 4364 5045 5236 826 Size: 219 2184 1135 681 191 1471 Fragments arranged by size: 2184 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5881 SmaI CCC'GGG Cuts at: 1872 3208 1872 Size: 1336 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3985 4886 5148 820 Size: 197 926 2042 901 262 1553 Fragments arranged by size: 2042 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3672 493 Size: 3179 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5881 SphI G_CATG'C Cuts at: 1132 3239 1132 Size: 2107 3774 SspI AAT'ATT Cuts at: 5 52 3805 5 Size: 47 3753 2081 Fragments arranged by size: 3753 2081 47 StuI AGG'CCT Cuts at: 1517 2307 1517 Size: 790 5091 StyI C'CwwG_G Cuts at: 513 1065 2004 3036 3171 3320 513 Size: 552 939 1032 135 149 3074 Fragments arranged by size: 3074 1032 939 552 149 135 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2839 3908 3925 4078 4263 4708 325 Size: 1255 1259 1069 17 153 185 445 1498 Fragments arranged by size: 1498 1259 1255 1069 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2935 4127 5839 Size: 274 80 33 51 1443 958 1192 1712 Cuts at: 5839 96 Size: 138 Fragments arranged by size: 1712 1443 1192 958 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5841 5873 5841 Size: 32 5849 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5881 XbaI T'CTAG_A Cuts at: 3217 3217 Size: 5881 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2248 2248 Size: 5881 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5881 XmaI C'CCGG_G Cuts at: 1870 3206 1870 Size: 1336 4545 XmnI GAAnn'nnTTC Cuts at: 2951 4010 2951 Size: 1059 4822 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3689 276 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI