Restriction map for
pFN21AB7590
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BbrPI BbsI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco52I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MamI
MfeI MluI MroI MseI MunI
NcoI NdeI NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TfiI Tru9I
Tth111I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AflIII 313 ACACGT A^C[GA][CT]GT
AlwI 768 GGATC GGATC(4/5)
AlwNI 846 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 365 AGCGCT AGC^GCT
AvaI 202 CCCGGG C^[CT]CG[GA]G
BalI 117 TGGCCA TGG^CCA
BbeI 13 GGCGCC GGCGC^C
BcgI 522 CGAGGCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 314 CACGTA [CT]AC^GT[GA]
BsiEI 24 CGACCG CG[GA][CT]^CG
BsoBI 202 CCCGGG C^[CT]CG[GA]G
BspMI 630 ACCTGC ACCTGC(4/8)
BssHII 37 GCGCGC G^CGCGC
BstEII 658 GGTGACC G^GT[ACGT]ACC
BstF5I 320 CATCC GGATG(2/0)
BstPI 658 GGTGACC G^GT[ACGT]ACC
BstXI 811 CCATACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 768 GGATCT [GA]^GATC[CT]
DpnII 769 GATC ^GATC
Eco47III 365 AGCGCT AGC^GCT
EcoNI 276 CCTGCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 658 GGTGACC G^GT[ACGT]ACC
FokI 320 CATCC GGATG(9/13)
HinfI 49 GAGTC G^A[ACGT]TC
KasI 13 GGCGCC G^GCGCC
MaeII 315 ACGT A^CGT
MaeIII 659 GTGAC ^GT[ACGT]AC
MboI 769 GATC ^GATC
MboII 471 GAAGA GAAGA(8/7)
MflI 768 GGATCT [GA]^GATC[CT]
MluNI 117 TGGCCA TGG^CCA
MscI 117 TGGCCA TGG^CCA
MslI 234 CATACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 13 GGCGCC GG^CGCC
NdeII 769 GATC ^GATC
PflMI 372 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 49 GAGTC GAGTC(4/5)
PpuMI 142 GGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 769 GATC ^GATC
SfaNI 247 GATGC GCATC(5/9)
SfoI 13 GGCGCC GGC^GCC
SmaI 202 CCCGGG CCC^GGG
TaqI 806 TCGA T^CGA
Tsp45I 659 GTGAC ^GT[GC]AC
TthHB8I 806 TCGA T^CGA
Van91I 372 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 768 GGATCT [GA]^GATC[CT]
XmaCI 202 CCCGGG C^CCGGG
XmaI 202 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 13 GGCGCC G[GA]^CG[CT]C
504 GGCGTC
AfaI 317 GTAC GT^AC
342 GTAC
AocI 280 CCTAAGG CC^T[ACGT]AGG
653 CCTTAGG
BanI 13 GGCGCC G^G[CT][GA]CC
646 GGCACC
BsaHI 13 GGCGCC G[GA]^CG[CT]C
504 GGCGTC
BseRI 510 CTCCTC GAGGAG(10/8)
672 CTCCTC
BsrBI 760 CCGCTC CCGCTC(-3/-3)
89 GAGCGG
Bsu36I 280 CCTAAGG CC^T[ACGT]AGG
653 CCTTAGG
EaeI 117 TGGCCA [CT]^GGCC[GA]
137 TGGCCG
Eco81I 280 CCTAAGG CC^T[ACGT]AGG
653 CCTTAGG
HgaI 505 GCGTC GACGC(5/10)
709 GCGTC
Hin1I 13 GGCGCC G[GA]^CG[CT]C
504 GGCGTC
HphI 435 GGTGA GGTGA(8/7)
658 GGTGA
NaeI 566 GCCGGC GCC^GGC
778 GCCGGC
NgoMIV 566 GCCGGC G^CCGGC
778 GCCGGC
RsaI 317 GTAC GT^AC
342 GTAC
StuI 524 AGGCCT AGG^CCT
624 AGGCCT
Tsp509I 261 AATT ^AATT
270 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AluI ApaI AvaII
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MaeI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV PvuII Sau96I ScrFI TseI
TspRI