Restriction map for
pFN21AB6988
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# NO CUT ENZYMES ( 169 enzymes) #
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Acc65I AccI AccII AciI AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MseI
MslI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 697 GACGTC GACGT^C
AccIII 300 TCCGGA T^CCGGA
AcyI 697 GACGTC G[GA]^CG[CT]C
AlwI 588 GGATC GGATC(4/5)
AlwNI 59 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 210 GGGCCC GGGCC^C
Asp700I 405 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 265 GGACC G^G[AT]CC
BanI 160 GGCACC G^G[CT][GA]CC
BbsI 567 GAAGAC GAAGAC(2/6)
BglI 478 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 567 GAAGAC GAAGAC(2/6)
BsaAI 359 CACGTA [CT]AC^GT[GA]
BsaHI 697 GACGTC G[GA]^CG[CT]C
BsaI 284 GAGACC GGTCTC(1/5)
BsaWI 300 TCCGGA [AT]^CCGG[AT]
BseAI 300 TCCGGA T^CCGGA
BsmAI 284 GAGAC GTCTC(1/5)
BsmI 498 GAATGC GAATGC(1/-1)
BspEI 300 TCCGGA T^CCGGA
BspMI 91 ACCTGC ACCTGC(4/8)
BstAPI 100 GCAGTGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 257 GGTCACC G^GT[ACGT]ACC
BstPI 257 GGTCACC G^GT[ACGT]ACC
BstYI 588 GGATCT [GA]^GATC[CT]
DpnII 589 GATC ^GATC
EcoO65I 257 GGTCACC G^GT[ACGT]ACC
HapII 301 CCGG C^CGG
HgaI 49 GCGTC GACGC(5/10)
Hin1I 697 GACGTC G[GA]^CG[CT]C
HinfI 525 GAATC G^A[ACGT]TC
HpaII 301 CCGG C^CGG
MboI 589 GATC ^GATC
MflI 588 GGATCT [GA]^GATC[CT]
MroI 300 TCCGGA T^CCGGA
MspI 301 CCGG C^CGG
NdeII 589 GATC ^GATC
Sau3AI 589 GATC ^GATC
SfiI 477 GGCCTCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI 525 GAATC G^A[AT]TC
XcmI 710 CCACCTCCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 588 GGATCT [GA]^GATC[CT]
XmnI 405 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AluI 115 AGCT AG^CT
339 AGCT
BalI 485 TGGCCA TGG^CCA
684 TGGCCA
BanII 193 GGGCTC G[GA]GC[CT]^C
210 GGGCCC
BmyI 193 GGGCTC G[AGT]GC[ACT]^C
210 GGGCCC
BpmI 119 CTCCAG CTGGAG(16/14)
233 CTCCAG
BsmFI 3 GTCCC GGGAC(10/14)
700 GTCCC
Bsp1286I 193 GGGCTC G[AGT]GC[ACT]^C
210 GGGCCC
BsrI 620 ACTGG ACTGG(1/-1)
687 CCAGT
BstF5I 78 GGATG GGATG(2/0)
303 GGATG
EcoT14I 164 CCAAGG C^C[AT][AT]GG
189 CCAAGG
FokI 78 GGATG GGATG(9/13)
303 GGATG
MaeII 360 ACGT A^CGT
698 ACGT
MaeIII 67 GTGAC ^GT[ACGT]AC
258 GTCAC
MluNI 485 TGGCCA TGG^CCA
684 TGGCCA
MscI 485 TGGCCA TGG^CCA
684 TGGCCA
PstI 98 CTGCAG CTGCA^G
503 CTGCAG
StuI 455 AGGCCT AGG^CCT
476 AGGCCT
StyI 164 CCAAGG C^C[AT][AT]GG
189 CCAAGG
Tsp45I 67 GTGAC ^GT[GC]AC
258 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
BbvI BsaJI BsiYI BslI BssKI
BstNI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII Fnu4HI HaeIII HphI
ItaI MboII MnlI MvaI MwoI
NlaIV Sau96I ScrFI SfaNI SfcI
TseI TspRI