Restriction map for pFN21AB6988
 (Circular) (Six-base) MAPSORT of: pFN21AB6988.seq  Check: 5553  from: 1  to: 5449



pFN21AB6988  5449 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:33  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2706    3259     278 
   Size:         53      83     186     936    1170     553    2468

  Fragments arranged by size: 

                2468    1170     936     553     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2792    1937 
   Size:        855    4594
 
Acc65I G'GTAC_C

Cuts at:   2770    2770 
   Size:       5449
 
AclI AA'CG_TT

Cuts at:   1563    3576    3949    1563 
   Size:       2013     373    3063

  Fragments arranged by size: 

                3063    2013     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3512    4896    5164    1632 
   Size:       1880    1384     268    1917

  Fragments arranged by size: 

                1917    1880    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5252
 
AflIII A'CryG_T

Cuts at:   1203    4348    5325    1203 
   Size:       3145     977    1327

  Fragments arranged by size: 

                3145    1327     977
 
AgeI A'CCGG_T

Cuts at:   4280    4280 
   Size:       5449
 
AhdI GACnn_n'nnGTC

Cuts at:   4178    4178 
   Size:       5449
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5449
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5449
 
AlwNI CAG_nnn'CTG

Cuts at:   2069    3192    4764    2069 
   Size:       1123    1572    2754

  Fragments arranged by size: 

                2754    1572    1123
 
ApaI G_GGCC'C

Cuts at:   2219    2219 
   Size:       5449
 
ApaLI G'TGCA_C

Cuts at:   3506    4662    3506 
   Size:       1156    4293
 
ApoI r'AATT_y

Cuts at:   1493    2758    2984    3136    5245    1493 
   Size:       1265     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1265     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4003     160 
   Size:        872    2971    1606

  Fragments arranged by size: 

                2971    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5449
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2774    1870 
   Size:         73     831    4545

  Fragments arranged by size: 

                4545     831      73
 
BamHI G'GATC_C

Cuts at:   2779    3146    2779 
   Size:        367    5082
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2165    2770    4225     618 
   Size:        325     217     627     378     605    1455    1842

  Fragments arranged by size: 

                1842    1455     627     605     378     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2202    2219    2768    1391 
   Size:        811      17     549    4072

  Fragments arranged by size: 

                4072     811     549      17
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2579     928 
   Size:        971     680    3798

  Fragments arranged by size: 

                3798     971     680
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3640    3674    1903 
   Size:         34    1703      34    3678

  Fragments arranged by size: 

                3678    1703      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3341    4551    3341 
   Size:       1210    4239
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5449
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2489    4060     136 
   Size:        107     122      71     631     605     817    1571    1525

  Fragments arranged by size: 

                1571    1525     817     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5444    5444 
   Size:       5449
 
BlpI GC'TnA_GC

Cuts at:   2866    2866 
   Size:       5449
 
Bme1580I G_kGCm'C

Cuts at:   1790    2219    3510    4666    1790 
   Size:        429    1291    1156    2573

  Fragments arranged by size: 

                2573    1291    1156     429
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5449
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3180    4138     442 
   Size:        416      53      83     399      24    1763     958    1753

  Fragments arranged by size: 

                1763    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5449
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2109    2223    4109    1239 
   Size:        870     114    1886    2579

  Fragments arranged by size: 

                2579    1886     870     114
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3574    4439    4737    3574 
   Size:        865     298    4286

  Fragments arranged by size: 

                4286     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2283    4112     882 
   Size:        617     784    1829    2219

  Fragments arranged by size: 

                2219    1829     784     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2366    3240     493 
   Size:        711    1162     874    2702

  Fragments arranged by size: 

                2702    1162     874     711
 
BsaBI GATnn'nnATC

Cuts at:   5443    5443 
   Size:       5449
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2703    3256    3638    4273 
   Size:         53      83     186     936    1170     553     382     635

Cuts at:   4273     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1170     936     635     553     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2305    3882    4280    4554    4701    5112    2305 
   Size:       1577     398     274     147     411    2642

  Fragments arranged by size: 

                2642    1577     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2013    2043    1178 
   Size:         30     165      30     610      30    4584

  Fragments arranged by size: 

                4584     610     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5449
 
BseYI C'CCAG_C

Cuts at:   4652    5148    4652 
   Size:        496    4953
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4876
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2899    3660    4688    1540 
   Size:        275     186     898     761    1028    2301

  Fragments arranged by size: 

                2301    1028     898     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3510    3595    4666    3510 
   Size:         85    1071    4293

  Fragments arranged by size: 

                4293    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2509    2960    3053    2509 
   Size:        451      93    4905

  Fragments arranged by size: 

                4905     451      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2202    2219    2768    3510    3595    4666    1391 
   Size:        399     412      17     549     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     549     412     399      85      17
            
 
BspEI T'CCGG_A

Cuts at:   2305    5112    2305 
   Size:       2807    2642
 
BspHI T'CATG_A

Cuts at:   3338    5068    3338 
   Size:       1730    3719
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2105    2804     844 
   Size:       1261     699    3489

  Fragments arranged by size: 

                3489    1261     699
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3336    1394 
   Size:        429    1513    3507

  Fragments arranged by size: 

                3507    1513     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3944    4118      65 
   Size:       1183    2696     174    1396

  Fragments arranged by size: 

                2696    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4093    4280    1700 
   Size:        110    2283     187    2869

  Fragments arranged by size: 

                2869    2283     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5449
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5449
 
BssSI C'ACGA_G

Cuts at:   3509    4521    3509 
   Size:       1012    4437
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2111     794 
   Size:       1317    4132
 
BstBI TT'CG_AA

Cuts at:   4253    4253 
   Size:       5449
 
BstEII G'GTnAC_C

Cuts at:   1184    2262    1184 
   Size:       1078    4371
 
BstYI r'GATC_y

Cuts at:   1972    2593    2779    3146    3531    3548    5000    5086    5098 
   Size:        621     186     367     385      17    1452      86      12

Cuts at:   5098    5444    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     621     385     367     346     186      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4897
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4710

  Fragments arranged by size: 

                4710     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2112    2965    3759    3779    2112 
   Size:        853     794      20    3782

  Fragments arranged by size: 

                3782     853     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5449
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5414
 
DraI TTT'AAA

Cuts at:   2753    2753 
   Size:       5449
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4456     809 
   Size:        507    3140    1802

  Fragments arranged by size: 

                3140    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2490    2689    2727 
   Size:         54    1425      50     275      18     660     199      38

Cuts at:   2727    2896    3785    5414       8 
   Size:        169     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     660     275     199     169      54
                  50      43      38      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2896    1537 
   Size:        275    1084    4090

  Fragments arranged by size: 

                4090    1084     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3379    1353 
   Size:       2026    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4036    4410    4556     387 
   Size:       1064     138    2447     374     146    1280

  Fragments arranged by size: 

                2447    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2109    2223    3512    4109    4896    5164    1239 
   Size:        393     477     114    1289     597     787     268    1524

  Fragments arranged by size: 

                1524    1289     787     597     477     393     268     114
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2215    2216    2234    2252    2215 
   Size:          1      18      18    5412

  Fragments arranged by size: 

                5412      18      18       1
 
EcoRI G'AATT_C

Cuts at:   2758    3136    2758 
   Size:        378    5071
 
FspI TGC'GCA

Cuts at:   3955    3955 
   Size:       5449
 
HaeII r_GCGC'y

Cuts at:   1678    4596    1678 
   Size:       2918    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2374    2406    4090    4122    4164 
   Size:         32     135      32     802      32    1684      32      42

Cuts at:   4164    4196    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1684     802     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2793    3039     669 
   Size:        988     281     855     246    3079

  Fragments arranged by size: 

                3079     988     855     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5449
 
HpaI GTT'AAC

Cuts at:   3039    3039 
   Size:       5449
 
KpnI G_GTAC'C

Cuts at:   2774    2774 
   Size:       5449
 
MfeI C'AATT_G

Cuts at:   3048    3048 
   Size:       5449
 
MluI A'CGCG_T

Cuts at:   5325    5325 
   Size:       5449
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4563    4747    1305 
   Size:       3258     184    2007

  Fragments arranged by size: 

                3258    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2492    2691      10 
   Size:         54    1425     343     660     199    2768

  Fragments arranged by size: 

                2768    1425     660     343     199      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3407    3766    3925     518 
   Size:        648    2241     359     159    2042

  Fragments arranged by size: 

                2241    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2865    3542    4690    4935    1606 
   Size:       1259     677    1148     245    2120

  Fragments arranged by size: 

                2120    1259    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4897
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5449
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5449
 
NotI GC'GGCC_GC

Cuts at:   2896    2896 
   Size:       5449
 
NspI r_CATG'y

Cuts at:   1132    1229    2807    4352    1132 
   Size:         97    1578    1545    2229

  Fragments arranged by size: 

                2229    1578    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5449
 
PciI A'CATG_T

Cuts at:   4348    4348 
   Size:       5449
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5449
 
PfoI T'CCnGG_A

Cuts at:   2239    2239 
   Size:       5449
 
PmeI GTTT'AAAC

Cuts at:   2753    2753 
   Size:       5449
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3518    3550    5031    5063    3518 
   Size:         32    1481      32    3904

  Fragments arranged by size: 

                3904    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5449
 
PsiI TTA'TAA

Cuts at:   3019    3019 
   Size:       5449
 
PspOMI G'GGCC_C

Cuts at:   2215    2215 
   Size:       5449
 
PstI C_TGCA'G

Cuts at:    830    2107    2512    2801     830 
   Size:       1277     405     289    3478

  Fragments arranged by size: 

                3478    1277     405     289
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5449
 
SalI G'TCGA_C

Cuts at:   1936    2791    1936 
   Size:        855    4594
 
SbfI CC_TGCA'GG

Cuts at:   2801    2801 
   Size:       5449
 
ScaI AGT'ACT

Cuts at:   3697    5409    3697 
   Size:       1712    3737
 
SfcI C'TryA_G

Cuts at:    826    1045    2103    2116    2508    2797    3932    4613    4804 
   Size:        219    1058      13     392     289    1135     681     191

Cuts at:   4804     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1135    1058     681     392     289     219     191
                  13
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2489    2489 
   Size:       5449
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5449
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5449
 
SmaI CCC'GGG

Cuts at:   1872    2776    1872 
   Size:        904    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3553    4454    4716     820 
   Size:        197     926    1610     901     262    1553

  Fragments arranged by size: 

                1610    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3240     493 
   Size:       2747    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5449
 
SphI G_CATG'C

Cuts at:   1132    2807    1132 
   Size:       1675    3774
 
SspI AAT'ATT

Cuts at:      5      52    3373       5 
   Size:         47    3321    2081

  Fragments arranged by size: 

                3321    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2462    2483    1517 
   Size:        945      21    4483

  Fragments arranged by size: 

                4483     945      21
 
StyI C'CwwG_G

Cuts at:    513    1065    2169    2194    2888     513 
   Size:        552    1104      25     694    3074

  Fragments arranged by size: 

                3074    1104     694     552      25
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3476    3493    3646    3831    4276     325 
   Size:       1255    1896      17     153     185     445    1498

  Fragments arranged by size: 

                1896    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3695    5407      96 
   Size:        274      80      33      51    1443    1718    1712     138

  Fragments arranged by size: 

                1718    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2013    2045    2620    2652    5409    5441    2013 
   Size:         32     575      32    2757      32    2021

  Fragments arranged by size: 

                2757    2021     575      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5449
 
XbaI T'CTAG_A

Cuts at:   2785    2785 
   Size:       5449
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2722    2722 
   Size:       5449
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5449
 
XmaI C'CCGG_G

Cuts at:   1870    2774    1870 
   Size:        904    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2414    3578    2414 
   Size:       1164    4285
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2704    3257     276 
   Size:         53      83     186     936    1170     553    2468

  Fragments arranged by size: 

                2468    1170     936     553     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI   PspOMI     PstI     PvuI     SalI
     SbfI     ScaI     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PmlI    PpuMI
    PspXI     PsrI    PvuII    RsrII     SacI    SacII    SanDI     SapI
    SexAI     SfoI     SrfI     SwaI
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