Restriction map for
pFN21AB6988
(Circular) (Six-base) MAPSORT of: pFN21AB6988.seq Check: 5553 from: 1 to: 5449
pFN21AB6988 5449 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2706 3259 278
Size: 53 83 186 936 1170 553 2468
Fragments arranged by size:
2468 1170 936 553 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2792 1937
Size: 855 4594
Acc65I G'GTAC_C
Cuts at: 2770 2770
Size: 5449
AclI AA'CG_TT
Cuts at: 1563 3576 3949 1563
Size: 2013 373 3063
Fragments arranged by size:
3063 2013 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3512 4896 5164 1632
Size: 1880 1384 268 1917
Fragments arranged by size:
1917 1880 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5252
AflIII A'CryG_T
Cuts at: 1203 4348 5325 1203
Size: 3145 977 1327
Fragments arranged by size:
3145 1327 977
AgeI A'CCGG_T
Cuts at: 4280 4280
Size: 5449
AhdI GACnn_n'nnGTC
Cuts at: 4178 4178
Size: 5449
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5449
AlwNI CAG_nnn'CTG
Cuts at: 2069 3192 4764 2069
Size: 1123 1572 2754
Fragments arranged by size:
2754 1572 1123
ApaI G_GGCC'C
Cuts at: 2219 2219
Size: 5449
ApaLI G'TGCA_C
Cuts at: 3506 4662 3506
Size: 1156 4293
ApoI r'AATT_y
Cuts at: 1493 2758 2984 3136 5245 1493
Size: 1265 226 152 2109 1697
Fragments arranged by size:
2109 1697 1265 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4003 160
Size: 872 2971 1606
Fragments arranged by size:
2971 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
AvaI C'yCGr_G
Cuts at: 1870 1943 2774 1870
Size: 73 831 4545
Fragments arranged by size:
4545 831 73
BamHI G'GATC_C
Cuts at: 2779 3146 2779
Size: 367 5082
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2165 2770 4225 618
Size: 325 217 627 378 605 1455 1842
Fragments arranged by size:
1842 1455 627 605 378 325 217
BanII G_rGCy'C
Cuts at: 1391 2202 2219 2768 1391
Size: 811 17 549 4072
Fragments arranged by size:
4072 811 549 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2579 928
Size: 971 680 3798
Fragments arranged by size:
3798 971 680
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3640 3674 1903
Size: 34 1703 34 3678
Fragments arranged by size:
3678 1703 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3341 4551 3341
Size: 1210 4239
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5449
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2489 4060 136
Size: 107 122 71 631 605 817 1571 1525
Fragments arranged by size:
1571 1525 817 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5444 5444
Size: 5449
BlpI GC'TnA_GC
Cuts at: 2866 2866
Size: 5449
Bme1580I G_kGCm'C
Cuts at: 1790 2219 3510 4666 1790
Size: 429 1291 1156 2573
Fragments arranged by size:
2573 1291 1156 429
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5449
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3180 4138 442
Size: 416 53 83 399 24 1763 958 1753
Fragments arranged by size:
1763 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2109 2223 4109 1239
Size: 870 114 1886 2579
Fragments arranged by size:
2579 1886 870 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3574 4439 4737 3574
Size: 865 298 4286
Fragments arranged by size:
4286 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2283 4112 882
Size: 617 784 1829 2219
Fragments arranged by size:
2219 1829 784 617
BsaAI yAC'GTr
Cuts at: 493 1204 2366 3240 493
Size: 711 1162 874 2702
Fragments arranged by size:
2702 1162 874 711
BsaBI GATnn'nnATC
Cuts at: 5443 5443
Size: 5449
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2703 3256 3638 4273
Size: 53 83 186 936 1170 553 382 635
Cuts at: 4273 275
Size: 1451
Fragments arranged by size:
1451 1170 936 635 553 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2305 3882 4280 4554 4701 5112 2305
Size: 1577 398 274 147 411 2642
Fragments arranged by size:
2642 1577 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2013 2043 1178
Size: 30 165 30 610 30 4584
Fragments arranged by size:
4584 610 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5449
BseYI C'CCAG_C
Cuts at: 4652 5148 4652
Size: 496 4953
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4876
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2899 3660 4688 1540
Size: 275 186 898 761 1028 2301
Fragments arranged by size:
2301 1028 898 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3510 3595 4666 3510
Size: 85 1071 4293
Fragments arranged by size:
4293 1071 85
BsmI GAATG_Cn'
Cuts at: 2509 2960 3053 2509
Size: 451 93 4905
Fragments arranged by size:
4905 451 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2219 2768 3510 3595 4666 1391
Size: 399 412 17 549 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 549 412 399 85 17
BspEI T'CCGG_A
Cuts at: 2305 5112 2305
Size: 2807 2642
BspHI T'CATG_A
Cuts at: 3338 5068 3338
Size: 1730 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2105 2804 844
Size: 1261 699 3489
Fragments arranged by size:
3489 1261 699
BsrBI CCG'CTC
Cuts at: 1394 1823 3336 1394
Size: 429 1513 3507
Fragments arranged by size:
3507 1513 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3944 4118 65
Size: 1183 2696 174 1396
Fragments arranged by size:
2696 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4093 4280 1700
Size: 110 2283 187 2869
Fragments arranged by size:
2869 2283 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5449
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5449
BssSI C'ACGA_G
Cuts at: 3509 4521 3509
Size: 1012 4437
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2111 794
Size: 1317 4132
BstBI TT'CG_AA
Cuts at: 4253 4253
Size: 5449
BstEII G'GTnAC_C
Cuts at: 1184 2262 1184
Size: 1078 4371
BstYI r'GATC_y
Cuts at: 1972 2593 2779 3146 3531 3548 5000 5086 5098
Size: 621 186 367 385 17 1452 86 12
Cuts at: 5098 5444 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 621 385 367 346 186 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4897
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4710
Fragments arranged by size:
4710 683 56
BtsI GCAGTG_nn'
Cuts at: 2112 2965 3759 3779 2112
Size: 853 794 20 3782
Fragments arranged by size:
3782 853 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5449
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5414
DraI TTT'AAA
Cuts at: 2753 2753
Size: 5449
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4456 809
Size: 507 3140 1802
Fragments arranged by size:
3140 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2490 2689 2727
Size: 54 1425 50 275 18 660 199 38
Cuts at: 2727 2896 3785 5414 8
Size: 169 889 1629 43
Fragments arranged by size:
1629 1425 889 660 275 199 169 54
50 43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 2896 1537
Size: 275 1084 4090
Fragments arranged by size:
4090 1084 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3379 1353
Size: 2026 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4036 4410 4556 387
Size: 1064 138 2447 374 146 1280
Fragments arranged by size:
2447 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2223 3512 4109 4896 5164 1239
Size: 393 477 114 1289 597 787 268 1524
Fragments arranged by size:
1524 1289 787 597 477 393 268 114
EcoO109I rG'GnC_Cy
Cuts at: 2215 2216 2234 2252 2215
Size: 1 18 18 5412
Fragments arranged by size:
5412 18 18 1
EcoRI G'AATT_C
Cuts at: 2758 3136 2758
Size: 378 5071
FspI TGC'GCA
Cuts at: 3955 3955
Size: 5449
HaeII r_GCGC'y
Cuts at: 1678 4596 1678
Size: 2918 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2374 2406 4090 4122 4164
Size: 32 135 32 802 32 1684 32 42
Cuts at: 4164 4196 1373
Size: 32 2626
Fragments arranged by size:
2626 1684 802 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2793 3039 669
Size: 988 281 855 246 3079
Fragments arranged by size:
3079 988 855 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5449
HpaI GTT'AAC
Cuts at: 3039 3039
Size: 5449
KpnI G_GTAC'C
Cuts at: 2774 2774
Size: 5449
MfeI C'AATT_G
Cuts at: 3048 3048
Size: 5449
MluI A'CGCG_T
Cuts at: 5325 5325
Size: 5449
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4563 4747 1305
Size: 3258 184 2007
Fragments arranged by size:
3258 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2492 2691 10
Size: 54 1425 343 660 199 2768
Fragments arranged by size:
2768 1425 660 343 199 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3407 3766 3925 518
Size: 648 2241 359 159 2042
Fragments arranged by size:
2241 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2865 3542 4690 4935 1606
Size: 1259 677 1148 245 2120
Fragments arranged by size:
2120 1259 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4897
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5449
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5449
NotI GC'GGCC_GC
Cuts at: 2896 2896
Size: 5449
NspI r_CATG'y
Cuts at: 1132 1229 2807 4352 1132
Size: 97 1578 1545 2229
Fragments arranged by size:
2229 1578 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5449
PciI A'CATG_T
Cuts at: 4348 4348
Size: 5449
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5449
PfoI T'CCnGG_A
Cuts at: 2239 2239
Size: 5449
PmeI GTTT'AAAC
Cuts at: 2753 2753
Size: 5449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 5031 5063 3518
Size: 32 1481 32 3904
Fragments arranged by size:
3904 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5449
PsiI TTA'TAA
Cuts at: 3019 3019
Size: 5449
PspOMI G'GGCC_C
Cuts at: 2215 2215
Size: 5449
PstI C_TGCA'G
Cuts at: 830 2107 2512 2801 830
Size: 1277 405 289 3478
Fragments arranged by size:
3478 1277 405 289
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5449
SalI G'TCGA_C
Cuts at: 1936 2791 1936
Size: 855 4594
SbfI CC_TGCA'GG
Cuts at: 2801 2801
Size: 5449
ScaI AGT'ACT
Cuts at: 3697 5409 3697
Size: 1712 3737
SfcI C'TryA_G
Cuts at: 826 1045 2103 2116 2508 2797 3932 4613 4804
Size: 219 1058 13 392 289 1135 681 191
Cuts at: 4804 826
Size: 1471
Fragments arranged by size:
1471 1135 1058 681 392 289 219 191
13
SfiI GGCCn_nnn'nGGCC
Cuts at: 2489 2489
Size: 5449
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5449
SmaI CCC'GGG
Cuts at: 1872 2776 1872
Size: 904 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3553 4454 4716 820
Size: 197 926 1610 901 262 1553
Fragments arranged by size:
1610 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3240 493
Size: 2747 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5449
SphI G_CATG'C
Cuts at: 1132 2807 1132
Size: 1675 3774
SspI AAT'ATT
Cuts at: 5 52 3373 5
Size: 47 3321 2081
Fragments arranged by size:
3321 2081 47
StuI AGG'CCT
Cuts at: 1517 2462 2483 1517
Size: 945 21 4483
Fragments arranged by size:
4483 945 21
StyI C'CwwG_G
Cuts at: 513 1065 2169 2194 2888 513
Size: 552 1104 25 694 3074
Fragments arranged by size:
3074 1104 694 552 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3476 3493 3646 3831 4276 325
Size: 1255 1896 17 153 185 445 1498
Fragments arranged by size:
1896 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3695 5407 96
Size: 274 80 33 51 1443 1718 1712 138
Fragments arranged by size:
1718 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2013 2045 2620 2652 5409 5441 2013
Size: 32 575 32 2757 32 2021
Fragments arranged by size:
2757 2021 575 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5449
XbaI T'CTAG_A
Cuts at: 2785 2785
Size: 5449
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2722 2722
Size: 5449
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5449
XmaI C'CCGG_G
Cuts at: 1870 2774 1870
Size: 904 4545
XmnI GAAnn'nnTTC
Cuts at: 2414 3578 2414
Size: 1164 4285
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2704 3257 276
Size: 53 83 186 936 1170 553 2468
Fragments arranged by size:
2468 1170 936 553 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfoI SrfI SwaI