Restriction map for
pFN21AB6988
(Linear) (Six-base) MAPSORT of: KIBB6988.seq Check: 7105 from: 1 to: 744
KIBB6988.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AatII G_ACGT'C
Cuts at: 0 701 744
Size: 701 43
AlwNI CAG_nnn'CTG
Cuts at: 0 64 744
Size: 64 680
ApaI G_GGCC'C
Cuts at: 0 214 744
Size: 214 530
BanI G'GyrC_C
Cuts at: 0 160 744
Size: 160 584
BanII G_rGCy'C
Cuts at: 0 197 214 744
Size: 197 17 530
Fragments arranged by size:
530 197 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 574 744
Size: 574 170
BglI GCCn_nnn'nGGC
Cuts at: 0 484 744
Size: 484 260
Bme1580I G_kGCm'C
Cuts at: 0 214 744
Size: 214 530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 218 744
Size: 104 114 526
Fragments arranged by size:
526 114 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 278 744
Size: 278 466
BsaAI yAC'GTr
Cuts at: 0 361 744
Size: 361 383
BsaHI Gr'CG_yC
Cuts at: 0 698 744
Size: 698 46
BsaWI w'CCGG_w
Cuts at: 0 300 744
Size: 300 444
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 8 38 744
Size: 8 30 706
Fragments arranged by size:
706 30 8
BsmI GAATG_Cn'
Cuts at: 0 504 744
Size: 504 240
Bsp1286I G_dGCh'C
Cuts at: 0 197 214 744
Size: 197 17 530
Fragments arranged by size:
530 197 17
BspEI T'CCGG_A
Cuts at: 0 300 744
Size: 300 444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 100 744
Size: 100 644
BstAPI GCAn_nnn'nTGC
Cuts at: 0 106 744
Size: 106 638
BstEII G'GTnAC_C
Cuts at: 0 257 744
Size: 257 487
BstYI r'GATC_y
Cuts at: 0 588 744
Size: 588 156
BtsI GCAGTG_nn'
Cuts at: 0 107 744
Size: 107 637
EaeI y'GGCC_r
Cuts at: 0 485 684 722 744
Size: 485 199 38 22
Fragments arranged by size:
485 199 38 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 218 744
Size: 104 114 526
Fragments arranged by size:
526 114 104
EcoO109I rG'GnC_Cy
Cuts at: 0 210 211 229 247 744
Size: 210 1 18 18 497
Fragments arranged by size:
497 210 18 18 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 369 401 744
Size: 369 32 343
Fragments arranged by size:
369 343 32
MscI TGG'CCA
Cuts at: 0 487 686 744
Size: 487 199 58
Fragments arranged by size:
487 199 58
PfoI T'CCnGG_A
Cuts at: 0 234 744
Size: 234 510
PspOMI G'GGCC_C
Cuts at: 0 210 744
Size: 210 534
PstI C_TGCA'G
Cuts at: 0 102 507 744
Size: 102 405 237
Fragments arranged by size:
405 237 102
SfcI C'TryA_G
Cuts at: 0 98 111 503 744
Size: 98 13 392 241
Fragments arranged by size:
392 241 98 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 484 744
Size: 484 260
StuI AGG'CCT
Cuts at: 0 457 478 744
Size: 457 21 266
Fragments arranged by size:
457 266 21
StyI C'CwwG_G
Cuts at: 0 164 189 744
Size: 164 25 555
Fragments arranged by size:
555 164 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 8 40 615 647 744
Size: 8 32 575 32 97
Fragments arranged by size:
575 97 32 32 8
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 717 744
Size: 717 27
XmnI GAAnn'nnTTC
Cuts at: 0 409 744
Size: 409 335
ZraI GAC'GTC
Cuts at: 0 699 744
Size: 699 45
Enzymes that do cut:
AatII AlwNI ApaI BanI BanII BbsI BglI Bme1580I
BpmI BsaI BsaAI BsaHI BsaWI BsaXI BsmI Bsp1286I
BspEI BspMI BstAPI BstEII BstYI BtsI EaeI Eco57MI
EcoO109I Hin4I MscI PfoI PspOMI PstI SfcI SfiI
StuI StyI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaBI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI Tth111I XbaI XhoI XmaI