(Circular) (Six-base) MAPSORT of: pFN21AB0994.seq Check: 8403 from: 1 to: 6088 pFN21AB0994 6088 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2005 2731 2005 Size: 726 5362 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2850 3431 1937 Size: 913 581 4594 Fragments arranged by size: 4594 913 581 Acc65I G'GTAC_C Cuts at: 3409 3409 Size: 6088 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2704 4151 5535 5803 1632 Size: 1072 1447 1384 268 1917 Fragments arranged by size: 1917 1447 1384 1072 268 AfeI AGC'GCT Cuts at: 3353 3353 Size: 6088 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 2043 2209 4987 5964 1203 Size: 840 166 2778 977 1327 Fragments arranged by size: 2778 1327 977 840 166 AgeI A'CCGG_T Cuts at: 4919 4919 Size: 6088 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6088 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6088 AlwNI CAG_nnn'CTG Cuts at: 2252 2794 3831 5403 2252 Size: 542 1037 1572 2937 Fragments arranged by size: 2937 1572 1037 542 ApaI G_GGCC'C Cuts at: 2519 3114 3165 2519 Size: 595 51 5442 Fragments arranged by size: 5442 595 51 ApaLI G'TGCA_C Cuts at: 2525 4145 5301 2525 Size: 1620 1156 3312 Fragments arranged by size: 3312 1620 1156 ApoI r'AATT_y Cuts at: 1493 3397 3623 3775 5884 1493 Size: 1904 226 152 2109 1697 Fragments arranged by size: 2109 1904 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4642 160 Size: 872 3610 1606 Fragments arranged by size: 3610 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 3413 1870 Size: 73 1470 4545 Fragments arranged by size: 4545 1470 73 BamHI G'GATC_C Cuts at: 3418 3785 3418 Size: 367 5721 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2271 3409 4864 618 Size: 325 217 627 484 1138 1455 1842 Fragments arranged by size: 1842 1455 1138 627 484 325 217 BanII G_rGCy'C Cuts at: 1391 2373 2519 2619 3045 3114 3165 3345 3407 Size: 982 146 100 426 69 51 180 62 Cuts at: 3407 1391 Size: 4072 Fragments arranged by size: 4072 982 426 180 146 100 69 62 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5117 BbvCI CC'TCA_GC Cuts at: 3132 3215 3132 Size: 83 6005 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4279 4313 1903 Size: 34 2342 34 3678 Fragments arranged by size: 3678 2342 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3980 5190 3980 Size: 1210 4878 BclI T'GATC_A Cuts at: 1548 2469 1548 Size: 921 5167 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3128 4699 136 Size: 107 122 71 631 605 1456 1571 1525 Fragments arranged by size: 1571 1525 1456 631 605 122 107 71 BglII A'GATC_T Cuts at: 6083 6083 Size: 6088 BlpI GC'TnA_GC Cuts at: 3505 3505 Size: 6088 Bme1580I G_kGCm'C Cuts at: 1790 2276 2492 2519 2529 3114 3165 4149 5305 Size: 486 216 27 10 585 51 984 1156 Cuts at: 5305 1790 Size: 2573 Fragments arranged by size: 2573 1156 984 585 486 216 51 27 10 BmgBI CAC'GTC Cuts at: 1318 2210 2485 1318 Size: 892 275 4921 Fragments arranged by size: 4921 892 275 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2502 2522 3819 Size: 416 53 83 399 24 1085 20 1297 Cuts at: 3819 4777 442 Size: 958 1753 Fragments arranged by size: 1753 1297 1085 958 416 399 83 53 24 20 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2011 2405 2575 2789 3005 3308 3323 3329 Size: 772 394 170 214 216 303 15 6 Cuts at: 3329 3386 4748 1239 Size: 57 1362 2579 Fragments arranged by size: 2579 1362 772 394 303 216 214 170 57 15 6 Bpu10I CC'TnA_GC Cuts at: 3132 3215 3132 Size: 83 6005 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3164 4213 5078 5376 3164 Size: 1049 865 298 3876 Fragments arranged by size: 3876 1049 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4751 882 Size: 617 3252 2219 Fragments arranged by size: 3252 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2044 3193 3879 493 Size: 711 840 1149 686 2702 Fragments arranged by size: 2702 1149 840 711 686 BsaBI GATnn'nnATC Cuts at: 6082 6082 Size: 6088 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3895 4277 4912 275 Size: 53 83 186 936 2362 382 635 1451 Fragments arranged by size: 2362 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2880 4521 4919 5193 5340 5751 2880 Size: 1641 398 274 147 411 3217 Fragments arranged by size: 3217 1641 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2765 2795 2909 2939 3077 Size: 30 165 30 1362 30 114 30 138 Cuts at: 3077 3107 1178 Size: 30 4159 Fragments arranged by size: 4159 1362 165 138 114 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2786 3149 3158 3320 3323 3383 1186 Size: 1600 363 9 162 3 60 3891 Fragments arranged by size: 3891 1600 363 162 60 9 3 BseYI C'CCAG_C Cuts at: 2949 3142 3158 3278 5291 5787 2949 Size: 193 16 120 2013 496 3250 Fragments arranged by size: 3250 2013 496 193 120 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2299 2472 2700 2900 3008 3356 1163 Size: 573 563 173 228 200 108 348 3895 Fragments arranged by size: 3895 573 563 348 228 200 173 108 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3538 4299 5327 1540 Size: 275 186 1537 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2529 3345 4149 4234 5305 2529 Size: 816 804 85 1071 3312 Fragments arranged by size: 3312 1071 816 804 85 BsmI GAATG_Cn' Cuts at: 2227 3599 3692 2227 Size: 1372 93 4623 Fragments arranged by size: 4623 1372 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2098 2098 Size: 6088 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2276 2373 2492 2519 2529 2619 3045 Size: 399 486 97 119 27 10 90 426 Cuts at: 3045 3114 3165 3345 3407 4149 4234 5305 1391 Size: 69 51 180 62 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 486 426 399 180 119 97 90 85 69 62 51 27 10 BspEI T'CCGG_A Cuts at: 5751 5751 Size: 6088 BspHI T'CATG_A Cuts at: 3977 5707 3977 Size: 1730 4358 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2005 2444 2731 3443 844 Size: 1161 439 287 712 3489 Fragments arranged by size: 3489 1161 712 439 287 BsrBI CCG'CTC Cuts at: 1394 1823 2025 2737 3031 3975 1394 Size: 429 202 712 294 944 3507 Fragments arranged by size: 3507 944 712 429 294 202 BsrDI GCAATG_nn' Cuts at: 65 1248 2292 3045 4583 4757 65 Size: 1183 1044 753 1538 174 1396 Fragments arranged by size: 1538 1396 1183 1044 753 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2010 2056 4732 4919 1700 Size: 110 200 46 2676 187 2869 Fragments arranged by size: 2869 2676 200 187 110 46 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6088 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6088 BssSI C'ACGA_G Cuts at: 2657 3026 4148 5160 2657 Size: 369 1122 1012 3585 Fragments arranged by size: 3585 1122 1012 369 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6088 BstBI TT'CG_AA Cuts at: 4892 4892 Size: 6088 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6088 BstXI CCAn_nnnn'nTGG Cuts at: 2048 2404 2048 Size: 356 5732 BstYI r'GATC_y Cuts at: 1972 2334 2703 3418 3785 4170 4187 5639 5725 Size: 362 369 715 367 385 17 1452 86 Cuts at: 5725 5737 6083 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 715 385 369 367 362 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2229 2712 3330 513 Size: 552 1164 483 618 3271 Fragments arranged by size: 3271 1164 618 552 483 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2248 487 Size: 683 56 1022 4327 Fragments arranged by size: 4327 1022 683 56 BtsI GCAGTG_nn' Cuts at: 2688 3604 4398 4418 2688 Size: 916 794 20 4358 Fragments arranged by size: 4358 916 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6053 DraI TTT'AAA Cuts at: 3392 3392 Size: 6088 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2318 5095 809 Size: 507 1002 2777 1802 Fragments arranged by size: 2777 1802 1002 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2239 2499 Size: 54 1425 50 275 18 228 181 260 Cuts at: 2499 3535 4424 6053 8 Size: 1036 889 1629 43 Fragments arranged by size: 1629 1425 1036 889 275 260 228 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3535 1537 Size: 275 1723 4090 Fragments arranged by size: 4090 1723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4018 1353 Size: 2665 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 4675 5049 5195 387 Size: 1064 138 438 2648 374 146 1280 Fragments arranged by size: 2648 1280 1064 438 374 146 138 EcoICRI GAG'CTC Cuts at: 3343 3343 Size: 6088 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2011 2405 2575 2704 2789 3005 3308 Size: 393 379 394 170 129 85 216 303 Cuts at: 3308 3323 3329 3386 4151 4748 5535 5803 1239 Size: 15 6 57 765 597 787 268 1524 Fragments arranged by size: 1524 787 765 597 394 393 379 303 268 216 170 129 85 57 15 6 EcoNI CCTnn'n_nnAGG Cuts at: 3080 3080 Size: 6088 EcoO109I rG'GnC_Cy Cuts at: 2175 2347 2664 2905 3088 3110 3162 3229 2175 Size: 172 317 241 183 22 52 67 5034 Fragments arranged by size: 5034 317 241 183 172 67 52 22 EcoRI G'AATT_C Cuts at: 3397 3775 3397 Size: 378 5710 FspI TGC'GCA Cuts at: 2606 4594 2606 Size: 1988 4100 FspAI rTGC'GCAy Cuts at: 2606 2606 Size: 6088 HaeII r_GCGC'y Cuts at: 1678 2750 3355 5235 1678 Size: 1072 605 1880 2531 Fragments arranged by size: 2531 1880 1072 605 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2765 2797 3046 3078 3278 Size: 32 135 32 1193 32 249 32 200 Cuts at: 3278 3310 4729 4761 4803 4835 1373 Size: 32 1419 32 42 32 2626 Fragments arranged by size: 2626 1419 1193 249 200 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2131 3223 3432 3678 669 Size: 988 281 193 1092 209 246 3079 Fragments arranged by size: 3079 1092 988 281 246 209 193 HindIII A'AGCT_T Cuts at: 748 748 Size: 6088 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KpnI G_GTAC'C Cuts at: 3413 3413 Size: 6088 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2673 3051 5202 5386 1305 Size: 1368 378 2151 184 2007 Fragments arranged by size: 2151 2007 1368 378 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2241 10 Size: 54 1425 343 409 3857 Fragments arranged by size: 3857 1425 409 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2321 3050 3056 4046 4405 4564 518 Size: 648 1155 729 6 990 359 159 2042 Fragments arranged by size: 2042 1155 990 729 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2166 2779 2808 2953 3504 4181 5329 5574 Size: 560 613 29 145 551 677 1148 245 Cuts at: 5574 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 613 560 551 245 145 29 NaeI GCC'GGC Cuts at: 2012 2058 2012 Size: 46 6042 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5536 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NgoMIV G'CCGG_C Cuts at: 2010 2056 2010 Size: 46 6042 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6088 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 2971 3446 4991 1132 Size: 97 1742 475 1545 2229 Fragments arranged by size: 2229 1742 1545 475 97 PasI CC'CwG_GG Cuts at: 1348 2194 1348 Size: 846 5242 PciI A'CATG_T Cuts at: 4987 4987 Size: 6088 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6088 PfoI T'CCnGG_A Cuts at: 3081 3081 Size: 6088 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PmlI CAC'GTG Cuts at: 2044 2044 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2664 3088 2664 Size: 424 5664 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6088 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PspOMI G'GGCC_C Cuts at: 2515 3110 3161 2515 Size: 595 51 5442 Fragments arranged by size: 5442 595 51 PstI C_TGCA'G Cuts at: 830 2790 3440 830 Size: 1960 650 3478 Fragments arranged by size: 3478 1960 650 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 SacI G_AGCT'C Cuts at: 3345 3345 Size: 6088 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SanDI GG'GwC_CC Cuts at: 2664 2664 Size: 6088 SbfI CC_TGCA'GG Cuts at: 2790 3440 2790 Size: 650 5438 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SfcI C'TryA_G Cuts at: 826 1045 2542 2786 3436 4571 5252 5443 826 Size: 219 1497 244 650 1135 681 191 1471 Fragments arranged by size: 1497 1471 1135 681 650 244 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6088 SmaI CCC'GGG Cuts at: 1872 3415 1872 Size: 1543 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3179 4192 5093 5355 820 Size: 197 926 1236 1013 901 262 1553 Fragments arranged by size: 1553 1236 1013 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3193 3879 493 Size: 2700 686 2702 Fragments arranged by size: 2702 2700 686 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 3446 1132 Size: 2314 3774 SspI AAT'ATT Cuts at: 5 52 4012 5 Size: 47 3960 2081 Fragments arranged by size: 3960 2081 47 StuI AGG'CCT Cuts at: 1517 2571 1517 Size: 1054 5034 StyI C'CwwG_G Cuts at: 513 1065 2061 2940 3384 3527 513 Size: 552 996 879 444 143 3074 Fragments arranged by size: 3074 996 879 552 444 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2544 3115 4115 4132 4285 4470 4915 Size: 1255 964 571 1000 17 153 185 445 Cuts at: 4915 325 Size: 1498 Fragments arranged by size: 1498 1255 1000 964 571 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4334 6046 96 Size: 274 80 33 51 1443 2357 1712 138 Fragments arranged by size: 2357 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2909 2941 6048 6080 2909 Size: 32 3107 32 2917 Fragments arranged by size: 3107 2917 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2288 1316 Size: 972 5116 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2509 2980 2509 Size: 471 5617 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6088 XmaI C'CCGG_G Cuts at: 1870 3413 1870 Size: 1543 4545 XmnI GAAnn'nnTTC Cuts at: 4217 4217 Size: 6088 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI KasI NarI NruI NsiI PacI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI