(Circular) (Six-base) MAPSORT of: pFN21AB0987.seq Check: 3383 from: 1 to: 5479 pFN21AB0987 5479 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3289 278 Size: 53 83 186 936 1753 2468 Fragments arranged by size: 2468 1753 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2454 2822 1937 Size: 517 368 4594 Fragments arranged by size: 4594 517 368 Acc65I G'GTAC_C Cuts at: 2800 2800 Size: 5479 AclI AA'CG_TT Cuts at: 1563 3606 3979 1563 Size: 2043 373 3063 Fragments arranged by size: 3063 2043 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 2384 2687 2744 3542 4926 5194 1632 Size: 455 297 303 57 798 1384 268 1917 Fragments arranged by size: 1917 1384 798 455 303 297 268 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5282 AflIII A'CryG_T Cuts at: 1203 2301 4378 5355 1203 Size: 1098 2077 977 1327 Fragments arranged by size: 2077 1327 1098 977 AgeI A'CCGG_T Cuts at: 4310 4310 Size: 5479 AhdI GACnn_n'nnGTC Cuts at: 4208 4208 Size: 5479 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5479 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5479 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2167 2199 2167 Size: 32 5447 AlwNI CAG_nnn'CTG Cuts at: 2147 2247 2440 2539 3222 4794 2147 Size: 100 193 99 683 1572 2832 Fragments arranged by size: 2832 1572 683 193 100 99 ApaLI G'TGCA_C Cuts at: 3536 4692 3536 Size: 1156 4323 ApoI r'AATT_y Cuts at: 1493 2788 3014 3166 5275 1493 Size: 1295 226 152 2109 1697 Fragments arranged by size: 2109 1697 1295 226 152 AseI AT'TA_AT Cuts at: 160 1032 4033 160 Size: 872 3001 1606 Fragments arranged by size: 3001 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5479 AvaI C'yCGr_G Cuts at: 1870 1943 2804 1870 Size: 73 861 4545 Fragments arranged by size: 4545 861 73 BamHI G'GATC_C Cuts at: 2809 3176 2809 Size: 367 5112 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2519 2800 4255 618 Size: 325 217 627 732 281 1455 1842 Fragments arranged by size: 1842 1455 732 627 325 281 217 BanII G_rGCy'C Cuts at: 1391 2798 1391 Size: 1407 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2146 928 Size: 971 247 4261 Fragments arranged by size: 4261 971 247 BbvCI CC'TCA_GC Cuts at: 2140 2336 2529 2140 Size: 196 193 5090 Fragments arranged by size: 5090 196 193 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3670 3704 1903 Size: 34 1733 34 3678 Fragments arranged by size: 3678 1733 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3371 4581 3371 Size: 1210 4269 BclI T'GATC_A Cuts at: 1548 1548 Size: 5479 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4090 136 Size: 107 122 71 631 605 2418 1525 Fragments arranged by size: 2418 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5474 5474 Size: 5479 BlpI GC'TnA_GC Cuts at: 2415 2896 2415 Size: 481 4998 Bme1580I G_kGCm'C Cuts at: 1790 2524 3540 4696 1790 Size: 734 1016 1156 2573 Fragments arranged by size: 2573 1156 1016 734 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5479 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2722 3210 4168 Size: 416 53 83 399 24 1305 488 958 Cuts at: 4168 442 Size: 1753 Fragments arranged by size: 1753 1305 958 488 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5479 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2155 2155 Size: 5479 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2495 2570 2575 4139 1239 Size: 1256 75 5 1564 2579 Fragments arranged by size: 2579 1564 1256 75 5 Bpu10I CC'TnA_GC Cuts at: 2140 2336 2529 2140 Size: 196 193 5090 Fragments arranged by size: 5090 196 193 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2393 3604 4469 4767 2393 Size: 1211 865 298 3105 Fragments arranged by size: 3105 1211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4142 882 Size: 617 2643 2219 Fragments arranged by size: 2643 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3270 493 Size: 711 2066 2702 Fragments arranged by size: 2702 2066 711 BsaBI GATnn'nnATC Cuts at: 5473 5473 Size: 5479 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3286 3668 4303 275 Size: 53 83 186 936 1753 382 635 1451 Fragments arranged by size: 1753 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3912 4310 4584 4731 5142 3912 Size: 398 274 147 411 4249 Fragments arranged by size: 4249 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5254 Fragments arranged by size: 5254 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2204 2267 1186 Size: 862 156 63 4398 Fragments arranged by size: 4398 862 156 63 BseYI C'CCAG_C Cuts at: 2480 2515 2539 4682 5178 2480 Size: 35 24 2143 496 2781 Fragments arranged by size: 2781 2143 496 35 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2260 1163 Size: 573 524 4382 Fragments arranged by size: 4382 573 524 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2929 3690 4718 1540 Size: 275 186 928 761 1028 2301 Fragments arranged by size: 2301 1028 928 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2084 2556 2701 3540 3625 4696 2084 Size: 472 145 839 85 1071 2867 Fragments arranged by size: 2867 1071 839 472 145 85 BsmI GAATG_Cn' Cuts at: 2285 2677 2990 3083 2285 Size: 392 313 93 4681 Fragments arranged by size: 4681 392 313 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 2524 2556 2701 2798 3540 3625 Size: 399 294 440 32 145 97 742 85 Cuts at: 3625 4696 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 440 399 294 145 97 85 32 BspEI T'CCGG_A Cuts at: 5142 5142 Size: 5479 BspHI T'CATG_A Cuts at: 2311 3368 5098 2311 Size: 1057 1730 2692 Fragments arranged by size: 2692 1730 1057 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2232 2834 844 Size: 1388 602 3489 Fragments arranged by size: 3489 1388 602 BsrBI CCG'CTC Cuts at: 1394 1823 3366 1394 Size: 429 1543 3507 Fragments arranged by size: 3507 1543 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2388 3974 4148 65 Size: 1183 1140 1586 174 1396 Fragments arranged by size: 1586 1396 1183 1140 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4123 4310 1700 Size: 110 2313 187 2869 Fragments arranged by size: 2869 2313 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5479 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5479 BssSI C'ACGA_G Cuts at: 3539 4551 3539 Size: 1012 4467 BstAPI GCAn_nnn'nTGC Cuts at: 794 2088 2440 794 Size: 1294 352 3833 Fragments arranged by size: 3833 1294 352 BstBI TT'CG_AA Cuts at: 4283 4283 Size: 5479 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5479 BstYI r'GATC_y Cuts at: 1972 2809 3176 3561 3578 5030 5116 5128 5474 Size: 837 367 385 17 1452 86 12 346 Cuts at: 5474 1972 Size: 1977 Fragments arranged by size: 1977 1452 837 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2458 513 Size: 552 1393 3534 Fragments arranged by size: 3534 1393 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4740 Fragments arranged by size: 4740 683 56 BtsI GCAGTG_nn' Cuts at: 2540 2995 3789 3809 2540 Size: 455 794 20 4210 Fragments arranged by size: 4210 794 455 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5479 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5444 DraI TTT'AAA Cuts at: 2783 2783 Size: 5479 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4486 809 Size: 507 3170 1802 Fragments arranged by size: 3170 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2571 2640 2926 Size: 54 1425 50 275 18 741 69 286 Cuts at: 2926 3815 5444 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 741 286 275 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2926 1537 Size: 275 1114 4090 Fragments arranged by size: 4090 1114 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2105 2225 3409 1353 Size: 752 120 1184 3423 Fragments arranged by size: 3423 1184 752 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4066 4440 4586 387 Size: 1064 138 2477 374 146 1280 Fragments arranged by size: 2477 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2384 2495 2570 2575 2687 2744 Size: 393 455 297 111 75 5 112 57 Cuts at: 2744 3542 4139 4926 5194 1239 Size: 798 597 787 268 1524 Fragments arranged by size: 1524 798 787 597 455 393 297 268 112 111 75 57 5 EcoNI CCTnn'n_nnAGG Cuts at: 2069 2069 Size: 5479 EcoRI G'AATT_C Cuts at: 2788 3166 2788 Size: 378 5101 FspI TGC'GCA Cuts at: 3985 3985 Size: 5479 HaeII r_GCGC'y Cuts at: 1678 4626 1678 Size: 2948 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4120 4152 4194 4226 1373 Size: 32 135 32 2548 32 42 32 2626 Fragments arranged by size: 2626 2548 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2823 3069 669 Size: 988 281 885 246 3079 Fragments arranged by size: 3079 988 885 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5479 HpaI GTT'AAC Cuts at: 3069 3069 Size: 5479 KpnI G_GTAC'C Cuts at: 2804 2804 Size: 5479 MfeI C'AATT_G Cuts at: 3078 3078 Size: 5479 MluI A'CGCG_T Cuts at: 5355 5355 Size: 5479 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2712 4593 4777 1305 Size: 1407 1881 184 2007 Fragments arranged by size: 2007 1881 1407 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3657 Fragments arranged by size: 3657 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3437 3796 3955 518 Size: 648 2271 359 159 2042 Fragments arranged by size: 2271 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2404 2515 2548 2895 3572 4720 4965 1606 Size: 798 111 33 347 677 1148 245 2120 Fragments arranged by size: 2120 1148 798 677 347 245 111 33 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4927 NdeI CA'TA_TG Cuts at: 387 387 Size: 5479 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5479 NotI GC'GGCC_GC Cuts at: 2926 2926 Size: 5479 NspI r_CATG'y Cuts at: 1132 1229 2129 2305 2560 2837 4382 1132 Size: 97 900 176 255 277 1545 2229 Fragments arranged by size: 2229 1545 900 277 255 176 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5479 PciI A'CATG_T Cuts at: 2301 4378 2301 Size: 2077 3402 PflMI CCAn_nnn'nTGG Cuts at: 1108 2473 1108 Size: 1365 4114 PmeI GTTT'AAAC Cuts at: 2783 2783 Size: 5479 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3548 3580 5061 5093 3548 Size: 32 1481 32 3934 Fragments arranged by size: 3934 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5479 PsiI TTA'TAA Cuts at: 3049 3049 Size: 5479 PstI C_TGCA'G Cuts at: 830 2436 2445 2831 830 Size: 1606 9 386 3478 Fragments arranged by size: 3478 1606 386 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5479 PvuII CAG'CTG Cuts at: 2515 2548 2515 Size: 33 5446 SalI G'TCGA_C Cuts at: 1936 2821 1936 Size: 885 4594 SbfI CC_TGCA'GG Cuts at: 2831 2831 Size: 5479 ScaI AGT'ACT Cuts at: 3727 5439 3727 Size: 1712 3767 SfcI C'TryA_G Cuts at: 826 1045 2432 2441 2827 3962 4643 4834 826 Size: 219 1387 9 386 1135 681 191 1471 Fragments arranged by size: 1471 1387 1135 681 386 219 191 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5479 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5479 SmaI CCC'GGG Cuts at: 1872 2806 1872 Size: 934 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2408 3583 4484 4746 820 Size: 197 926 465 1175 901 262 1553 Fragments arranged by size: 1553 1175 926 901 465 262 197 SnaBI TAC'GTA Cuts at: 493 3270 493 Size: 2777 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5479 SphI G_CATG'C Cuts at: 1132 2837 1132 Size: 1705 3774 SspI AAT'ATT Cuts at: 5 52 3403 5 Size: 47 3351 2081 Fragments arranged by size: 3351 2081 47 StuI AGG'CCT Cuts at: 1517 2138 1517 Size: 621 4858 StyI C'CwwG_G Cuts at: 513 1065 2643 2918 513 Size: 552 1578 275 3074 Fragments arranged by size: 3074 1578 552 275 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2507 3506 3523 3676 3861 4306 325 Size: 1255 927 999 17 153 185 445 1498 Fragments arranged by size: 1498 1255 999 927 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3725 5437 96 Size: 274 80 33 51 1443 1748 1712 138 Fragments arranged by size: 1748 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5439 5471 5439 Size: 32 5447 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5479 XbaI T'CTAG_A Cuts at: 2815 2815 Size: 5479 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2512 2512 Size: 5479 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5479 XmaI C'CCGG_G Cuts at: 1870 2804 1870 Size: 934 4545 XmnI GAAnn'nnTTC Cuts at: 2754 3608 2754 Size: 854 4625 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3287 276 Size: 53 83 186 936 1753 2468 Fragments arranged by size: 2468 1753 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI