Restriction map for
pFN21AB0967
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AviII BamHI BbrPI BbsI BcgI
BciVI BclI BfrI BglI BglII
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Cfr10I
ClaI CpoI DpnII DraI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MamI MboI MboII
MfeI MflI MluI MroI MseI
MslI MunI NaeI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI Sau3AI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tsp509I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AocI 17 CCTTAGG CC^T[ACGT]AGG
AvrII 207 CCTAGG C^CTAGG
BalI 137 TGGCCA TGG^CCA
BcnI 198 CCCGG CC^[GC]GG
BlnI 207 CCTAGG C^CTAGG
BlpI 283 GCTGAGC GC^T[ACGT]AGC
BpmI 90 CTCCAG CTGGAG(16/14)
Bpu1102I 283 GCTGAGC GC^T[ACGT]AGC
BsmAI 452 GAGAC GTCTC(1/5)
BsmFI 427 GTCCC GGGAC(10/14)
BsrDI 396 GCAATG GCAATG(2/0)
BstXI 259 CCAGGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 17 CCTTAGG CC^T[ACGT]AGG
CelII 283 GCTGAGC GC^T[ACGT]AGC
EaeI 137 TGGCCA [CT]^GGCC[GA]
Eco81I 17 CCTTAGG CC^T[ACGT]AGG
EcoNI 379 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 199 CCGG C^CGG
HgaI 409 GCGTC GACGC(5/10)
HinfI 178 GACTC G^A[ACGT]TC
HpaII 199 CCGG C^CGG
MaeII 350 ACGT A^CGT
MluNI 137 TGGCCA TGG^CCA
MscI 137 TGGCCA TGG^CCA
MspA1I 584 CAGCTG C[AC]G^C[GT]G
MspI 199 CCGG C^CGG
NciI 198 CCCGG CC^[GC]GG
NlaIII 604 CATG CATG^
PleI 178 GACTC GAGTC(4/5)
PpuMI 425 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 584 CAGCTG CAG^CTG
SbfI 366 CCTGCAGG CCTGCA^GG
SexAI 473 ACCTGGT A^CC[AT]GGT
SfaNI 30 GATGC GCATC(5/9)
SmlI 310 CTTGAG C^T[CT][GA]AG
Sse8387I 366 CCTGCAGG CCTGCA^GG
XcmI 348 CCACGTCGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AspI 346 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
472 GACCTGGTC
BbeI 161 GGCGCC GGCGC^C
236 GGCGCC
BfaI 148 CTAG C^TAG
208 CTAG
BsgI 12 CTGCAC GTGCAG(16/14)
538 CTGCAC
BspMI 536 ACCTGC ACCTGC(4/8)
423 GCAGGT
BsrI 142 ACTGG ACTGG(1/-1)
153 ACTGG
EcoT14I 53 CCTTGG C^C[AT][AT]GG
207 CCTAGG
FauI 203 CCCGC CCCGC(4/6)
435 GCGGG
HphI 101 TCACC GGTGA(8/7)
608 TCACC
KasI 161 GGCGCC G^GCGCC
236 GGCGCC
MaeI 148 CTAG C^TAG
208 CTAG
NarI 161 GGCGCC GG^CGCC
236 GGCGCC
PflFI 346 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
472 GACCTGGTC
PstI 271 CTGCAG CTGCA^G
367 CTGCAG
SfcI 271 CTGCAG C^T[GA][CT]AG
367 CTGCAG
SfoI 161 GGCGCC GGC^GCC
236 GGCGCC
StyI 53 CCTTGG C^C[AT][AT]GG
207 CCTAGG
Tth111I 346 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
472 GACCTGGTC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BanI BanII BbvCI BbvI
BmyI BsaHI BsaJI BsiYI BslI
BsoBI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HhaI Hin1I HinP1I
ItaI MaeIII MnlI MvaI MvnI
MwoI NlaIV Sau96I ScrFI TseI
Tsp45I TspRI