(Circular) (Six-base) MAPSORT of: pFN21AB0962.seq Check: 6074 from: 1 to: 5980 pFN21AB0962 5980 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3790 278 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3323 1937 Size: 1386 4594 Acc65I G'GTAC_C Cuts at: 3301 3301 Size: 5980 AclI AA'CG_TT Cuts at: 1563 4107 4480 1563 Size: 2544 373 3063 Fragments arranged by size: 3063 2544 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2729 3051 4043 5427 5695 1632 Size: 1097 322 992 1384 268 1917 Fragments arranged by size: 1917 1384 1097 992 322 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5783 AflIII A'CryG_T Cuts at: 1203 4879 5856 1203 Size: 3676 977 1327 Fragments arranged by size: 3676 1327 977 AgeI A'CCGG_T Cuts at: 4811 4811 Size: 5980 AhdI GACnn_n'nnGTC Cuts at: 4709 4709 Size: 5980 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5980 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5980 AlwNI CAG_nnn'CTG Cuts at: 2743 3723 5295 2743 Size: 980 1572 3428 Fragments arranged by size: 3428 1572 980 ApaLI G'TGCA_C Cuts at: 4037 5193 4037 Size: 1156 4824 ApoI r'AATT_y Cuts at: 1493 2555 2593 2879 3289 3515 3667 5776 1493 Size: 1062 38 286 410 226 152 2109 1697 Fragments arranged by size: 2109 1697 1062 410 286 226 152 38 AseI AT'TA_AT Cuts at: 160 1032 4534 160 Size: 872 3502 1606 Fragments arranged by size: 3502 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 AvaI C'yCGr_G Cuts at: 1870 1943 2290 3011 3305 1870 Size: 73 347 721 294 4545 Fragments arranged by size: 4545 721 347 294 73 BamHI G'GATC_C Cuts at: 2544 3310 3677 2544 Size: 766 367 4847 Fragments arranged by size: 4847 766 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2295 3301 4756 618 Size: 325 217 627 508 1006 1455 1842 Fragments arranged by size: 1842 1455 1006 627 508 325 217 BanII G_rGCy'C Cuts at: 1391 3012 3173 3216 3299 1391 Size: 1621 161 43 83 4072 Fragments arranged by size: 4072 1621 161 83 43 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2503 2717 3191 928 Size: 971 604 214 474 3717 Fragments arranged by size: 3717 971 604 474 214 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2387 2421 4171 4205 1903 Size: 34 450 34 1750 34 3678 Fragments arranged by size: 3678 1750 450 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3172 3872 5082 3172 Size: 700 1210 4070 Fragments arranged by size: 4070 1210 700 BclI T'GATC_A Cuts at: 1548 1548 Size: 5980 BfrBI ATG'CAT Cuts at: 2121 2121 Size: 5980 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2734 4591 136 Size: 107 122 71 631 605 1062 1857 1525 Fragments arranged by size: 1857 1525 1062 631 605 122 107 71 BglII A'GATC_T Cuts at: 5975 5975 Size: 5980 BlpI GC'TnA_GC Cuts at: 3397 3397 Size: 5980 Bme1580I G_kGCm'C Cuts at: 1790 2103 4041 5197 1790 Size: 313 1938 1156 2573 Fragments arranged by size: 2573 1938 1156 313 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5980 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3711 4669 442 Size: 416 53 83 399 24 2294 958 1753 Fragments arranged by size: 2294 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5980 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2765 2840 2879 4640 1239 Size: 1526 75 39 1761 2579 Fragments arranged by size: 2579 1761 1526 75 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2672 4105 4970 5268 2672 Size: 1433 865 298 3384 Fragments arranged by size: 3384 1433 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4643 882 Size: 617 3144 2219 Fragments arranged by size: 3144 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3771 493 Size: 711 2567 2702 Fragments arranged by size: 2702 2567 711 BsaBI GATnn'nnATC Cuts at: 5974 5974 Size: 5980 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3787 4169 4804 275 Size: 53 83 186 936 2254 382 635 1451 Fragments arranged by size: 2254 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4413 4811 5085 5232 5643 4413 Size: 398 274 147 411 4750 Fragments arranged by size: 4750 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5755 Fragments arranged by size: 5755 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2597 2600 3224 1186 Size: 1411 3 624 3942 Fragments arranged by size: 3942 1411 624 3 BseYI C'CCAG_C Cuts at: 2620 2961 3104 5183 5679 2620 Size: 341 143 2079 496 2921 Fragments arranged by size: 2921 2079 496 341 143 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2282 2788 1163 Size: 573 546 506 4355 Fragments arranged by size: 4355 573 546 506 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3430 4191 5219 1540 Size: 275 186 1429 761 1028 2301 Fragments arranged by size: 2301 1429 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3173 3216 4041 4126 5197 3173 Size: 43 825 85 1071 3956 Fragments arranged by size: 3956 1071 825 85 43 BsmI GAATG_Cn' Cuts at: 3491 3584 3491 Size: 93 5887 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 3012 3173 3216 3299 4041 4126 Size: 399 313 909 161 43 83 742 85 Cuts at: 4126 5197 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 909 742 399 313 161 85 83 43 BspEI T'CCGG_A Cuts at: 5643 5643 Size: 5980 BspHI T'CATG_A Cuts at: 2626 3869 5599 2626 Size: 1243 1730 3007 Fragments arranged by size: 3007 1730 1243 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3335 844 Size: 2491 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3867 1394 Size: 429 2044 3507 Fragments arranged by size: 3507 2044 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4475 4649 65 Size: 1183 3227 174 1396 Fragments arranged by size: 3227 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4624 4811 1700 Size: 110 2814 187 2869 Fragments arranged by size: 2869 2814 187 110 BsrGI T'GTAC_A Cuts at: 96 2494 96 Size: 2398 3582 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5980 BssSI C'ACGA_G Cuts at: 4040 5052 4040 Size: 1012 4968 BstAPI GCAn_nnn'nTGC Cuts at: 794 2725 794 Size: 1931 4049 BstBI TT'CG_AA Cuts at: 4784 4784 Size: 5980 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5980 BstXI CCAn_nnnn'nTGG Cuts at: 2062 2062 Size: 5980 BstYI r'GATC_y Cuts at: 1972 2439 2544 3310 3677 4062 4079 5531 5617 Size: 467 105 766 367 385 17 1452 86 Cuts at: 5617 5629 5975 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 766 467 385 367 346 105 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4489 Fragments arranged by size: 4489 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5241 Fragments arranged by size: 5241 683 56 BtsI GCAGTG_nn' Cuts at: 3496 4290 4310 3496 Size: 794 20 5166 Fragments arranged by size: 5166 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5980 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5945 DraI TTT'AAA Cuts at: 3284 3284 Size: 5980 DraIII CAC_nnn'GTG Cuts at: 2492 3230 2492 Size: 738 5242 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4987 809 Size: 507 3671 1802 Fragments arranged by size: 3671 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3427 4316 Size: 54 1425 50 275 18 177 1420 889 Cuts at: 4316 5945 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1420 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3427 1537 Size: 275 1615 4090 Fragments arranged by size: 4090 1615 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2772 2951 3070 3910 1353 Size: 1419 179 119 840 3423 Fragments arranged by size: 3423 1419 840 179 119 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4567 4941 5087 387 Size: 1064 138 2978 374 146 1280 Fragments arranged by size: 2978 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3171 3214 3171 Size: 43 5937 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2729 2765 2840 2879 3051 4043 4640 Size: 393 1097 36 75 39 172 992 597 Cuts at: 4640 5427 5695 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1097 992 787 597 393 268 172 75 39 36 EcoNI CCTnn'n_nnAGG Cuts at: 2582 2582 Size: 5980 EcoRI G'AATT_C Cuts at: 3289 3667 3289 Size: 378 5602 EcoRV GAT'ATC Cuts at: 2752 2752 Size: 5980 FspI TGC'GCA Cuts at: 4486 4486 Size: 5980 HaeII r_GCGC'y Cuts at: 1678 5127 1678 Size: 3449 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2249 2281 2360 2392 2999 Size: 32 135 32 677 32 79 32 607 Cuts at: 2999 3031 3142 3174 4621 4653 4695 4727 1373 Size: 32 111 32 1447 32 42 32 2626 Fragments arranged by size: 2626 1447 677 607 135 111 79 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3324 3570 669 Size: 988 281 1386 246 3079 Fragments arranged by size: 3079 1386 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5980 HpaI GTT'AAC Cuts at: 3570 3570 Size: 5980 KpnI G_GTAC'C Cuts at: 3305 3305 Size: 5980 MfeI C'AATT_G Cuts at: 3579 3579 Size: 5980 MluI A'CGCG_T Cuts at: 5856 5856 Size: 5980 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5094 5278 1305 Size: 3789 184 2007 Fragments arranged by size: 3789 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3981 Fragments arranged by size: 3981 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3938 4297 4456 518 Size: 648 2772 359 159 2042 Fragments arranged by size: 2772 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2740 3396 4073 5221 5466 1606 Size: 1134 656 677 1148 245 2120 Fragments arranged by size: 2120 1148 1134 677 656 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4489 Fragments arranged by size: 4489 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5980 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5980 NotI GC'GGCC_GC Cuts at: 3427 3427 Size: 5980 NsiI A_TGCA'T Cuts at: 2123 2123 Size: 5980 NspI r_CATG'y Cuts at: 1132 1229 2289 2728 3338 4883 1132 Size: 97 1060 439 610 1545 2229 Fragments arranged by size: 2229 1545 1060 610 439 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5980 PciI A'CATG_T Cuts at: 4879 4879 Size: 5980 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5980 PmeI GTTT'AAAC Cuts at: 3284 3284 Size: 5980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2987 3019 4049 4081 5562 5594 2987 Size: 32 1030 32 1481 32 3373 Fragments arranged by size: 3373 1481 1030 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5980 PsiI TTA'TAA Cuts at: 3550 3550 Size: 5980 PstI C_TGCA'G Cuts at: 830 2076 3028 3332 830 Size: 1246 952 304 3478 Fragments arranged by size: 3478 1246 952 304 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5980 PvuII CAG'CTG Cuts at: 2740 2740 Size: 5980 SacI G_AGCT'C Cuts at: 3173 3216 3173 Size: 43 5937 SalI G'TCGA_C Cuts at: 1936 3322 1936 Size: 1386 4594 SapI GCTCTTCn'nnn_ Cuts at: 2951 2951 Size: 5980 SbfI CC_TGCA'GG Cuts at: 3332 3332 Size: 5980 ScaI AGT'ACT Cuts at: 4228 5940 4228 Size: 1712 4268 SexAI A'CCwGG_T Cuts at: 2520 2520 Size: 5980 SfcI C'TryA_G Cuts at: 826 1045 2072 3024 3328 4463 5144 5335 826 Size: 219 1027 952 304 1135 681 191 1471 Fragments arranged by size: 1471 1135 1027 952 681 304 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5980 SmaI CCC'GGG Cuts at: 1872 3013 3307 1872 Size: 1141 294 4545 Fragments arranged by size: 4545 1141 294 SmlI C'TyrA_G Cuts at: 820 1017 1943 2687 4084 4985 5247 820 Size: 197 926 744 1397 901 262 1553 Fragments arranged by size: 1553 1397 926 901 744 262 197 SnaBI TAC'GTA Cuts at: 493 3771 493 Size: 3278 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5980 SphI G_CATG'C Cuts at: 1132 3338 1132 Size: 2206 3774 SspI AAT'ATT Cuts at: 5 52 3904 5 Size: 47 3852 2081 Fragments arranged by size: 3852 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5980 StyI C'CwwG_G Cuts at: 513 1065 2004 2364 3419 513 Size: 552 939 360 1055 3074 Fragments arranged by size: 3074 1055 939 552 360 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2746 4007 4024 4177 4362 4807 325 Size: 1255 1166 1261 17 153 185 445 1498 Fragments arranged by size: 1498 1261 1255 1166 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2473 2494 4226 Size: 274 80 33 51 1443 496 21 1732 Cuts at: 4226 5938 96 Size: 1712 138 Fragments arranged by size: 1732 1712 1443 496 274 138 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5940 5972 5940 Size: 32 5948 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5980 XbaI T'CTAG_A Cuts at: 3316 3316 Size: 5980 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2740 2740 Size: 5980 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5980 XmaI C'CCGG_G Cuts at: 1870 3011 3305 1870 Size: 1141 294 4545 Fragments arranged by size: 4545 1141 294 XmnI GAAnn'nnTTC Cuts at: 2594 2781 2879 4109 2594 Size: 187 98 1230 4465 Fragments arranged by size: 4465 1230 187 98 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3788 276 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI