Restriction map for
pFN21AB0961
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DpnII
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HapII HgaI Hin1I
HindIII HpaI HpaII KasI KpnI
KspI MaeI MaeII MaeIII MamI
MboI MfeI MflI MluI MroI
MseI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp45I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AciI 385 GCGG CCGC(-3/-1)
AcsI 322 AAATTT [GA]^AATT[CT]
AfaI 137 GTAC GT^AC
Aor51HI 350 AGCGCT AGC^GCT
ApoI 322 AAATTT [GA]^AATT[CT]
Asp700I 61 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 567 GGTCC G^G[AT]CC
BalI 288 TGGCCA TGG^CCA
BanI 232 GGCACC G^G[CT][GA]CC
BbsI 42 GAAGAC GAAGAC(2/6)
BbvCI 237 CCTCAGC CCTCAGC(-5/-2)
BpuAI 42 GAAGAC GAAGAC(2/6)
BsrI 8 ACTGG ACTGG(1/-1)
BssSI 274 CACGAG CACGAG(-5/-1)
BstXI 330 CCAGAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 559 GGGGCCT [GA]G^G[ACGT]CC[CT]
EarI 61 GAAGAG CTCTTC(1/4)
Eco47III 350 AGCGCT AGC^GCT
EcoO109I 559 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 314 ATGCAT ATGCA^T
HaeII 350 AGCGCT [GA]GCGC^[CT]
HincII 462 GTCAAC GT[CT]^[GA]AC
HindII 462 GTCAAC GT[CT]^[GA]AC
Ksp632I 61 GAAGAG CTCTTC(1/4)
MluNI 288 TGGCCA TGG^CCA
MscI 288 TGGCCA TGG^CCA
NsiI 314 ATGCAT ATGCA^T
RsaI 137 GTAC GT^AC
SmlI 457 CTTGAG C^T[CT][GA]AG
TaqI 432 TCGA T^CGA
TfiI 126 GAATC G^A[AT]TC
Tsp509I 323 AATT ^AATT
TthHB8I 432 TCGA T^CGA
XcmI 330 CCAGAAGATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 61 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 85 AGCT AG^CT
555 AGCT
ApaI 199 GGGCCC GGGCC^C
526 GGGCCC
BanII 199 GGGCCC G[GA]GC[CT]^C
526 GGGCCC
BmyI 199 GGGCCC G[AGT]GC[ACT]^C
526 GGGCCC
BseRI 14 GAGGAG GAGGAG(10/8)
160 GAGGAG
BsiYI 4 CCGAACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
105 CCTGTGCATGG
BslI 4 CCGAACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
105 CCTGTGCATGG
Bsp1286I 199 GGGCCC G[AGT]GC[ACT]^C
526 GGGCCC
Cac8I 229 GCAGGC GC[ACGT]^[ACGT]GC
424 GCCTGC
CfoI 227 GCGC GCG^C
351 GCGC
DdeI 238 CTCAG C^T[ACGT]AG
575 CTGAG
EaeI 146 TGGCCG [CT]^GGCC[GA]
288 TGGCCA
EcoT14I 530 CCAAGG C^C[AT][AT]GG
563 CCTTGG
HhaI 227 GCGC GCG^C
351 GCGC
HinP1I 227 GCGC G^CGC
351 GCGC
HphI 157 GGTGA GGTGA(8/7)
393 GGTGA
NlaIII 111 CATG CATG^
154 CATG
PleI 256 GAGTC GAGTC(4/5)
460 GAGTC
SfaNI 362 GATGC GCATC(5/9)
316 GCATC
StuI 422 AGGCCT AGG^CCT
453 AGGCCT
StyI 530 CCAAGG C^C[AT][AT]GG
563 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
BbvI BsaJI BssKI BstAPI BstF5I
BstNI Cfr13I EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MboII MnlI
MvaI MwoI NlaIV Sau96I ScrFI
TseI TspRI