(Circular) (Six-base) MAPSORT of: pFN21AB6666.seq Check: 3158 from: 1 to: 6064 pFN21AB6666 6064 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3874 278 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2171 2190 2622 3407 1937 Size: 234 19 432 785 4594 Fragments arranged by size: 4594 785 432 234 19 Acc65I G'GTAC_C Cuts at: 3385 3385 Size: 6064 AclI AA'CG_TT Cuts at: 1563 4191 4564 1563 Size: 2628 373 3063 Fragments arranged by size: 3063 2628 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2910 4127 5511 5779 1632 Size: 1278 1217 1384 268 1917 Fragments arranged by size: 1917 1384 1278 1217 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5867 AflIII A'CryG_T Cuts at: 1203 2072 4963 5940 1203 Size: 869 2891 977 1327 Fragments arranged by size: 2891 1327 977 869 AgeI A'CCGG_T Cuts at: 4895 4895 Size: 6064 AhdI GACnn_n'nnGTC Cuts at: 4793 4793 Size: 6064 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6064 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6064 AlwNI CAG_nnn'CTG Cuts at: 3024 3807 5379 3024 Size: 783 1572 3709 Fragments arranged by size: 3709 1572 783 ApaLI G'TGCA_C Cuts at: 4121 5277 4121 Size: 1156 4908 ApoI r'AATT_y Cuts at: 1493 2029 2756 3373 3599 3751 5860 1493 Size: 536 727 617 226 152 2109 1697 Fragments arranged by size: 2109 1697 727 617 536 226 152 AseI AT'TA_AT Cuts at: 160 1032 4618 160 Size: 872 3586 1606 Fragments arranged by size: 3586 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 AvaI C'yCGr_G Cuts at: 1870 1943 3389 1870 Size: 73 1446 4545 Fragments arranged by size: 4545 1446 73 BamHI G'GATC_C Cuts at: 3394 3761 3394 Size: 367 5697 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3385 4840 618 Size: 325 217 627 1598 1455 1842 Fragments arranged by size: 1842 1598 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3383 1391 Size: 1992 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2220 2841 928 Size: 971 321 621 4151 Fragments arranged by size: 4151 971 621 321 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4255 4289 1903 Size: 34 2318 34 3678 Fragments arranged by size: 3678 2318 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3956 5166 3956 Size: 1210 4854 BclI T'GATC_A Cuts at: 1548 1548 Size: 6064 BfrBI ATG'CAT Cuts at: 2816 2816 Size: 6064 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4675 136 Size: 107 122 71 631 605 3003 1525 Fragments arranged by size: 3003 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6059 6059 Size: 6064 BlpI GC'TnA_GC Cuts at: 3481 3481 Size: 6064 Bme1580I G_kGCm'C Cuts at: 1790 4125 5281 1790 Size: 2335 1156 2573 Fragments arranged by size: 2573 2335 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6064 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2538 3795 4753 Size: 416 53 83 399 24 1121 1257 958 Cuts at: 4753 442 Size: 1753 Fragments arranged by size: 1753 1257 1121 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6064 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2961 4724 1239 Size: 1722 1763 2579 Fragments arranged by size: 2579 1763 1722 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2380 4189 5054 5352 2380 Size: 1809 865 298 3092 Fragments arranged by size: 3092 1809 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4727 882 Size: 617 3228 2219 Fragments arranged by size: 3228 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3855 493 Size: 711 2651 2702 Fragments arranged by size: 2702 2651 711 BsaBI GATnn'nnATC Cuts at: 6058 6058 Size: 6064 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3871 4253 4888 275 Size: 53 83 186 936 2338 382 635 1451 Fragments arranged by size: 2338 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4497 4895 5169 5316 5727 4497 Size: 398 274 147 411 4834 Fragments arranged by size: 4834 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5839 Fragments arranged by size: 5839 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6064 BseYI C'CCAG_C Cuts at: 2469 2764 5267 5763 2469 Size: 295 2503 496 2770 Fragments arranged by size: 2770 2503 496 295 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5491 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3514 4275 5303 1540 Size: 275 186 1513 761 1028 2301 Fragments arranged by size: 2301 1513 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3318 4125 4210 5281 3318 Size: 807 85 1071 4101 Fragments arranged by size: 4101 1071 807 85 BsmI GAATG_Cn' Cuts at: 3575 3668 3575 Size: 93 5971 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3318 3383 4125 4210 5281 1391 Size: 399 1528 65 742 85 1071 2174 Fragments arranged by size: 2174 1528 1071 742 399 85 65 BspEI T'CCGG_A Cuts at: 5727 5727 Size: 6064 BspHI T'CATG_A Cuts at: 3953 5683 3953 Size: 1730 4334 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3419 844 Size: 2575 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2574 3951 1394 Size: 429 751 1377 3507 Fragments arranged by size: 3507 1377 751 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2154 2547 4559 4733 65 Size: 1183 906 393 2012 174 1396 Fragments arranged by size: 2012 1396 1183 906 393 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4708 4895 1700 Size: 110 2898 187 2869 Fragments arranged by size: 2898 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6064 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6064 BssSI C'ACGA_G Cuts at: 4124 5136 4124 Size: 1012 5052 BstAPI GCAn_nnn'nTGC Cuts at: 794 2087 2867 794 Size: 1293 780 3991 Fragments arranged by size: 3991 1293 780 BstBI TT'CG_AA Cuts at: 4868 4868 Size: 6064 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6064 BstXI CCAn_nnnn'nTGG Cuts at: 3116 3116 Size: 6064 BstYI r'GATC_y Cuts at: 1972 2197 3074 3394 3761 4146 4163 5615 5701 Size: 225 877 320 367 385 17 1452 86 Cuts at: 5701 5713 6059 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 877 385 367 346 320 225 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3351 3351 Size: 6064 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5512 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2985 487 Size: 683 56 1759 3566 Fragments arranged by size: 3566 1759 683 56 BtsI GCAGTG_nn' Cuts at: 2689 3580 4374 4394 2689 Size: 891 794 20 4359 Fragments arranged by size: 4359 891 794 20 ClaI AT'CG_AT Cuts at: 1554 2378 2634 1554 Size: 824 256 4984 Fragments arranged by size: 4984 824 256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6029 DraI TTT'AAA Cuts at: 3368 3368 Size: 6064 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5071 809 Size: 507 3755 1802 Fragments arranged by size: 3755 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3106 3511 4400 Size: 54 1425 50 275 18 1276 405 889 Cuts at: 4400 6029 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1276 889 405 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3511 1537 Size: 275 1699 4090 Fragments arranged by size: 4090 1699 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2071 2663 3994 1353 Size: 718 592 1331 3423 Fragments arranged by size: 3423 1331 718 592 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4651 5025 5171 387 Size: 1064 138 3062 374 146 1280 Fragments arranged by size: 3062 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2910 2961 4127 4724 5511 5779 1239 Size: 393 1278 51 1166 597 787 268 1524 Fragments arranged by size: 1524 1278 1166 787 597 393 268 51 EcoO109I rG'GnC_Cy Cuts at: 2885 2885 Size: 6064 EcoRI G'AATT_C Cuts at: 3373 3751 3373 Size: 378 5686 EcoRV GAT'ATC Cuts at: 2524 2524 Size: 6064 FspI TGC'GCA Cuts at: 4570 4570 Size: 6064 HaeII r_GCGC'y Cuts at: 1678 5211 1678 Size: 3533 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2153 2185 2189 2221 2285 Size: 32 135 32 581 32 4 32 64 Cuts at: 2285 2317 4705 4737 4779 4811 1373 Size: 32 2388 32 42 32 2626 Fragments arranged by size: 2626 2388 581 135 64 42 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 2449 3408 3654 669 Size: 988 281 511 959 246 3079 Fragments arranged by size: 3079 988 959 511 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6064 HpaI GTT'AAC Cuts at: 2449 3654 2449 Size: 1205 4859 KpnI G_GTAC'C Cuts at: 3389 3389 Size: 6064 MfeI C'AATT_G Cuts at: 3261 3663 3261 Size: 402 5662 MluI A'CGCG_T Cuts at: 5940 5940 Size: 6064 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2165 2299 5178 5362 1305 Size: 860 134 2879 184 2007 Fragments arranged by size: 2879 2007 860 184 134 MscI TGG'CCA Cuts at: 10 64 1489 1832 3108 10 Size: 54 1425 343 1276 2966 Fragments arranged by size: 2966 1425 1276 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3114 4022 4381 4540 518 Size: 648 1948 908 359 159 2042 Fragments arranged by size: 2042 1948 908 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2260 3480 4157 5305 5550 1606 Size: 654 1220 677 1148 245 2120 Fragments arranged by size: 2120 1220 1148 677 654 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5512 NdeI CA'TA_TG Cuts at: 387 2083 2812 2914 387 Size: 1696 729 102 3537 Fragments arranged by size: 3537 1696 729 102 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6064 NotI GC'GGCC_GC Cuts at: 3511 3511 Size: 6064 NsiI A_TGCA'T Cuts at: 2818 2818 Size: 6064 NspI r_CATG'y Cuts at: 1132 1229 2076 3013 3422 4967 1132 Size: 97 847 937 409 1545 2229 Fragments arranged by size: 2229 1545 937 847 409 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6064 PciI A'CATG_T Cuts at: 2072 4963 2072 Size: 2891 3173 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 5162 PfoI T'CCnGG_A Cuts at: 2339 2339 Size: 6064 PmeI GTTT'AAAC Cuts at: 3368 3368 Size: 6064 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4133 4165 5646 5678 4133 Size: 32 1481 32 4519 Fragments arranged by size: 4519 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2885 2885 Size: 6064 PshAI GACnn'nnGTC Cuts at: 1613 2734 1613 Size: 1121 4943 PsiI TTA'TAA Cuts at: 2286 3634 2286 Size: 1348 4716 PstI C_TGCA'G Cuts at: 830 2863 3416 830 Size: 2033 553 3478 Fragments arranged by size: 3478 2033 553 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6064 PvuII CAG'CTG Cuts at: 2260 2260 Size: 6064 SalI G'TCGA_C Cuts at: 1936 3406 1936 Size: 1470 4594 SbfI CC_TGCA'GG Cuts at: 3416 3416 Size: 6064 ScaI AGT'ACT Cuts at: 2857 4312 6024 2857 Size: 1455 1712 2897 Fragments arranged by size: 2897 1712 1455 SfcI C'TryA_G Cuts at: 826 1045 2201 2859 3412 4547 5228 5419 826 Size: 219 1156 658 553 1135 681 191 1471 Fragments arranged by size: 1471 1156 1135 681 658 553 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6064 SmaI CCC'GGG Cuts at: 1872 3391 1872 Size: 1519 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2395 4168 5069 5331 820 Size: 197 926 452 1773 901 262 1553 Fragments arranged by size: 1773 1553 926 901 452 262 197 SnaBI TAC'GTA Cuts at: 493 3855 493 Size: 3362 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6064 SphI G_CATG'C Cuts at: 1132 3013 3422 1132 Size: 1881 409 3774 Fragments arranged by size: 3774 1881 409 SspI AAT'ATT Cuts at: 5 52 3988 5 Size: 47 3936 2081 Fragments arranged by size: 3936 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6064 StyI C'CwwG_G Cuts at: 513 1065 3130 3503 513 Size: 552 2065 373 3074 Fragments arranged by size: 3074 2065 552 373 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4091 4108 4261 4446 4891 325 Size: 1255 2511 17 153 185 445 1498 Fragments arranged by size: 2511 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2855 4310 6022 Size: 274 80 33 51 1443 878 1455 1712 Cuts at: 6022 96 Size: 138 Fragments arranged by size: 1712 1455 1443 878 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2272 2304 2643 2675 6024 6056 2272 Size: 32 339 32 3349 32 2280 Fragments arranged by size: 3349 2280 339 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6064 XbaI T'CTAG_A Cuts at: 3400 3400 Size: 6064 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2525 2878 2525 Size: 353 5711 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6064 XmaI C'CCGG_G Cuts at: 1870 3389 1870 Size: 1519 4545 XmnI GAAnn'nnTTC Cuts at: 4193 4193 Size: 6064 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3872 276 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI