(Circular) (Six-base) MAPSORT of: pFN21AB0956.seq Check: 1957 from: 1 to: 5959 pFN21AB0956 5959 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3302 1937 Size: 1365 4594 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2441 2918 4022 5406 5674 1632 Size: 809 477 1104 1384 268 1917 Fragments arranged by size: 1917 1384 1104 809 477 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 2025 4858 5835 1203 Size: 822 2833 977 1327 Fragments arranged by size: 2833 1327 977 822 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 2955 1166 Size: 1789 4170 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3027 1184 Size: 1843 4116 AlwNI CAG_nnn'CTG Cuts at: 2263 2287 2387 2407 2891 3158 3702 5274 2263 Size: 24 100 20 484 267 544 1572 2948 Fragments arranged by size: 2948 1572 544 484 267 100 24 20 ApaLI G'TGCA_C Cuts at: 2305 4016 5172 2305 Size: 1711 1156 3092 Fragments arranged by size: 3092 1711 1156 ApoI r'AATT_y Cuts at: 1493 2639 3042 3268 3494 3646 5755 1493 Size: 1146 403 226 226 152 2109 1697 Fragments arranged by size: 2109 1697 1146 403 226 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 3284 1870 Size: 73 1341 4545 Fragments arranged by size: 4545 1341 73 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3280 4735 618 Size: 325 217 627 1493 1455 1842 Fragments arranged by size: 1842 1493 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3278 1391 Size: 1887 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4988 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4150 4184 1903 Size: 34 2213 34 3678 Fragments arranged by size: 3678 2213 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4570 136 Size: 107 122 71 631 605 337 2561 1525 Fragments arranged by size: 2561 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 2233 2309 2414 2964 4020 5176 1790 Size: 443 76 105 550 1056 1156 2573 Fragments arranged by size: 2573 1156 1056 550 443 105 76 BmgBI CAC'GTC Cuts at: 1318 2026 1318 Size: 708 5251 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2572 3690 4648 Size: 416 53 83 399 24 1155 1118 958 Cuts at: 4648 442 Size: 1753 Fragments arranged by size: 1753 1155 1118 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2703 2837 2913 3038 4619 1239 Size: 1464 134 76 125 1581 2579 Fragments arranged by size: 2579 1581 1464 134 125 76 Bpu10I CC'TnA_GC Cuts at: 2786 2786 Size: 5959 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4084 4949 5247 4084 Size: 865 298 4796 Fragments arranged by size: 4796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2071 2135 2739 2966 4622 882 Size: 617 572 64 604 227 1656 2219 Fragments arranged by size: 2219 1656 617 604 572 227 64 BsaAI yAC'GTr Cuts at: 493 1204 3750 493 Size: 711 2546 2702 Fragments arranged by size: 2702 2546 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2850 3766 4148 4783 Size: 53 83 186 936 1317 916 382 635 Cuts at: 4783 275 Size: 1451 Fragments arranged by size: 1451 1317 936 916 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2130 2730 4392 4790 5064 5211 5622 2130 Size: 600 1662 398 274 147 411 2467 Fragments arranged by size: 2467 1662 600 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2015 2045 1178 Size: 30 165 30 612 30 5092 Fragments arranged by size: 5092 612 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 2485 2621 3212 3215 3218 3221 1186 Size: 1075 224 136 591 3 3 3 3924 Fragments arranged by size: 3924 1075 591 224 136 3 3 3 BseYI C'CCAG_C Cuts at: 3235 5162 5658 3235 Size: 1927 496 3536 Fragments arranged by size: 3536 1927 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2157 2385 1163 Size: 573 421 228 4737 Fragments arranged by size: 4737 573 421 228 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3409 4170 5198 1540 Size: 275 186 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2309 4020 4105 5176 2309 Size: 1711 85 1071 3092 Fragments arranged by size: 3092 1711 1071 85 BsmI GAATG_Cn' Cuts at: 2194 2257 3470 3563 2194 Size: 63 1213 93 4590 Fragments arranged by size: 4590 1213 93 63 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2233 2309 2414 2964 3278 4020 4105 Size: 399 443 76 105 550 314 742 85 Cuts at: 4105 5176 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 550 443 399 314 105 85 76 BspEI T'CCGG_A Cuts at: 2130 5622 2130 Size: 3492 2467 BspHI T'CATG_A Cuts at: 3848 5578 3848 Size: 1730 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3314 844 Size: 2470 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3846 1394 Size: 429 2023 3507 Fragments arranged by size: 3507 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2341 4454 4628 65 Size: 1183 1093 2113 174 1396 Fragments arranged by size: 2113 1396 1183 1093 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4603 4790 1700 Size: 110 2793 187 2869 Fragments arranged by size: 2869 2793 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 2058 1925 Size: 133 5826 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstXI CCAn_nnnn'nTGG Cuts at: 3148 3148 Size: 5959 BstYI r'GATC_y Cuts at: 1972 2915 3102 3289 3656 4041 4058 5510 5596 Size: 943 187 187 367 385 17 1452 86 Cuts at: 5596 5608 5954 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 943 385 367 346 187 187 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2225 2829 3107 2225 Size: 604 278 5077 Fragments arranged by size: 5077 604 278 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 513 Size: 552 939 66 4402 Fragments arranged by size: 4402 939 552 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 2218 2368 3009 3475 4269 4289 2218 Size: 150 641 466 794 20 3888 Fragments arranged by size: 3888 794 641 466 150 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2526 2761 3406 Size: 54 1425 50 275 18 696 235 645 Cuts at: 3406 4295 5924 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 696 645 275 235 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2598 2678 3177 3183 3189 3889 1353 Size: 1245 80 499 6 6 700 3423 Fragments arranged by size: 3423 1245 700 499 80 6 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4546 4920 5066 387 Size: 1064 138 2957 374 146 1280 Fragments arranged by size: 2957 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2441 2703 2837 2913 2918 3038 4022 Size: 393 809 262 134 76 5 120 984 Cuts at: 4022 4619 5406 5674 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 984 809 787 597 393 268 262 134 120 76 5 EcoNI CCTnn'n_nnAGG Cuts at: 2690 3152 2690 Size: 462 5497 EcoO109I rG'GnC_Cy Cuts at: 2404 2417 2683 2404 Size: 13 266 5680 Fragments arranged by size: 5680 266 13 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FspI TGC'GCA Cuts at: 2315 4465 2315 Size: 2150 3809 HaeII r_GCGC'y Cuts at: 1678 2097 5106 1678 Size: 419 3009 2531 Fragments arranged by size: 3009 2531 419 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 2907 2939 4600 Size: 32 135 32 443 32 860 32 1661 Cuts at: 4600 4632 4674 4706 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1661 860 443 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3303 3549 669 Size: 988 281 1365 246 3079 Fragments arranged by size: 3079 1365 988 281 246 HindIII A'AGCT_T Cuts at: 748 2453 748 Size: 1705 4254 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2540 5073 5257 1305 Size: 1235 2533 184 2007 Fragments arranged by size: 2533 2007 1235 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2528 2763 10 Size: 54 1425 343 696 235 3206 Fragments arranged by size: 3206 1425 696 343 235 54 MslI CAynn'nnrTG Cuts at: 518 1166 2510 2805 2955 3917 4276 4435 518 Size: 648 1344 295 150 962 359 159 2042 Fragments arranged by size: 2042 1344 962 648 359 295 159 150 MspA1I CmG'CkG Cuts at: 1606 3073 3375 4052 5200 5445 1606 Size: 1467 302 677 1148 245 2120 Fragments arranged by size: 2120 1467 1148 677 302 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4468 Fragments arranged by size: 4468 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 3008 3317 4862 1132 Size: 97 1779 309 1545 2229 Fragments arranged by size: 2229 1779 1545 309 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2404 2404 Size: 5959 PshAI GACnn'nnGTC Cuts at: 1613 3066 1613 Size: 1453 4506 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PstI C_TGCA'G Cuts at: 830 2363 3311 830 Size: 1533 948 3478 Fragments arranged by size: 3478 1533 948 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SexAI A'CCwGG_T Cuts at: 2400 2400 Size: 5959 SfcI C'TryA_G Cuts at: 826 1045 2359 3307 4442 5123 5314 826 Size: 219 1314 948 1135 681 191 1471 Fragments arranged by size: 1471 1314 1135 948 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4063 4964 5226 820 Size: 197 926 2120 901 262 1553 Fragments arranged by size: 2120 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 2004 2097 2764 3398 513 Size: 552 939 93 667 634 3074 Fragments arranged by size: 3074 939 667 634 552 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2310 3986 4003 4156 4341 4786 325 Size: 1255 730 1676 17 153 185 445 1498 Fragments arranged by size: 1676 1498 1255 730 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2355 4205 5917 Size: 274 80 33 51 1443 378 1850 1712 Cuts at: 5917 96 Size: 138 Fragments arranged by size: 1850 1712 1443 378 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2112 2144 5919 5951 2112 Size: 32 3775 32 2120 Fragments arranged by size: 3775 2120 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI