Restriction map for
pFN21AB0956
(Linear) (Six-base) MAPSORT of: KIBB0956.seq Check: 6698 from: 1 to: 1254
KIBB0956.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 913 1254
Size: 436 477 341
Fragments arranged by size:
477 436 341
AflIII A'CryG_T
Cuts at: 0 20 1254
Size: 20 1234
AleI CACnn'nnGTG
Cuts at: 0 950 1254
Size: 950 304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1022 1254
Size: 1022 232
AlwNI CAG_nnn'CTG
Cuts at: 0 258 282 382 402 886 1153 1254
Size: 258 24 100 20 484 267 101
Fragments arranged by size:
484 267 258 101 100 24 20
ApaLI G'TGCA_C
Cuts at: 0 300 1254
Size: 300 954
ApoI r'AATT_y
Cuts at: 0 634 1037 1254
Size: 634 403 217
Fragments arranged by size:
634 403 217
Bme1580I G_kGCm'C
Cuts at: 0 228 304 409 959 1254
Size: 228 76 105 550 295
Fragments arranged by size:
550 295 228 105 76
BmgBI CAC'GTC
Cuts at: 0 21 1254
Size: 21 1233
BmrI ACTGGGnnnn_n'
Cuts at: 0 567 1254
Size: 567 687
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 832 908 1033 1254
Size: 698 134 76 125 221
Fragments arranged by size:
698 221 134 125 76
Bpu10I CC'TnA_GC
Cuts at: 0 781 1254
Size: 781 473
BsaI GGTCTCn'nnnn_
Cuts at: 0 66 130 734 961 1254
Size: 66 64 604 227 293
Fragments arranged by size:
604 293 227 66 64
BsaHI Gr'CG_yC
Cuts at: 0 845 1254
Size: 845 409
BsaWI w'CCGG_w
Cuts at: 0 125 725 1254
Size: 125 600 529
Fragments arranged by size:
600 529 125
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 10 40 1254
Size: 10 30 1214
Fragments arranged by size:
1214 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 480 616 1207 1210 1213 1216 1254
Size: 256 224 136 591 3 3 3 38
Fragments arranged by size:
591 256 224 136 38 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1230 1254
Size: 1230 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 380 1254
Size: 152 228 874
Fragments arranged by size:
874 228 152
BsiHKAI G_wGCw'C
Cuts at: 0 304 1254
Size: 304 950
BsmI GAATG_Cn'
Cuts at: 0 189 252 1254
Size: 189 63 1002
Fragments arranged by size:
1002 189 63
Bsp1286I G_dGCh'C
Cuts at: 0 228 304 409 959 1254
Size: 228 76 105 550 295
Fragments arranged by size:
550 295 228 105 76
BspEI T'CCGG_A
Cuts at: 0 125 1254
Size: 125 1129
BsrDI GCAATG_nn'
Cuts at: 0 336 1254
Size: 336 918
BssHII G'CGCG_C
Cuts at: 0 53 1254
Size: 53 1201
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1143 1254
Size: 1143 111
BstYI r'GATC_y
Cuts at: 0 910 1097 1254
Size: 910 187 157
Fragments arranged by size:
910 187 157
Bsu36I CC'TnA_GG
Cuts at: 0 220 824 1102 1254
Size: 220 604 278 152
Fragments arranged by size:
604 278 220 152
BtgI C'CryG_G
Cuts at: 0 65 1254
Size: 65 1189
BtsI GCAGTG_nn'
Cuts at: 0 213 363 1004 1254
Size: 213 150 641 250
Fragments arranged by size:
641 250 213 150
EaeI y'GGCC_r
Cuts at: 0 521 756 1254
Size: 521 235 498
Fragments arranged by size:
521 498 235
EarI CTCTTCn'nnn_
Cuts at: 0 593 673 1172 1178 1184 1254
Size: 593 80 499 6 6 70
Fragments arranged by size:
593 499 80 70 6 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 698 832 908 913 1033 1254
Size: 436 262 134 76 5 120 221
Fragments arranged by size:
436 262 221 134 120 76 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 685 1147 1254
Size: 685 462 107
Fragments arranged by size:
685 462 107
EcoO109I rG'GnC_Cy
Cuts at: 0 399 412 678 1254
Size: 399 13 266 576
Fragments arranged by size:
576 399 266 13
FspI TGC'GCA
Cuts at: 0 310 1254
Size: 310 944
HaeII r_GCGC'y
Cuts at: 0 92 1254
Size: 92 1162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 902 934 1254
Size: 10 32 860 32 320
Fragments arranged by size:
860 320 32 32 10
HindIII A'AGCT_T
Cuts at: 0 448 1254
Size: 448 806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 1254
Size: 535 719
MscI TGG'CCA
Cuts at: 0 523 758 1254
Size: 523 235 496
Fragments arranged by size:
523 496 235
MslI CAynn'nnrTG
Cuts at: 0 505 800 950 1254
Size: 505 295 150 304
Fragments arranged by size:
505 304 295 150
MspA1I CmG'CkG
Cuts at: 0 1068 1254
Size: 1068 186
NspI r_CATG'y
Cuts at: 0 1003 1254
Size: 1003 251
PpuMI rG'GwC_Cy
Cuts at: 0 399 1254
Size: 399 855
PshAI GACnn'nnGTC
Cuts at: 0 1061 1254
Size: 1061 193
PstI C_TGCA'G
Cuts at: 0 358 1254
Size: 358 896
SexAI A'CCwGG_T
Cuts at: 0 395 1254
Size: 395 859
SfcI C'TryA_G
Cuts at: 0 354 1254
Size: 354 900
StyI C'CwwG_G
Cuts at: 0 92 759 1254
Size: 92 667 495
Fragments arranged by size:
667 495 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 305 1254
Size: 305 949
TatI w'GTAC_w
Cuts at: 0 350 1254
Size: 350 904
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 107 139 1254
Size: 107 32 1115
Fragments arranged by size:
1115 107 32
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApaLI ApoI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BsrDI
BssHII BstXI BstYI Bsu36I BtgI BtsI EaeI EarI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HindIII MmeI
MscI MslI MspA1I NspI PpuMI PshAI PstI SexAI
SfcI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmtI BplI
BpuEI BsaAI BsaBI BsiEI BsiWI BsmBI BspHI BspMI
BsrBI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI FspAI HincII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI