(Circular) (Six-base) MAPSORT of: pFN21AB0950.seq Check: 2828 from: 1 to: 5926 pFN21AB0950 5926 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3269 1937 Size: 1332 4594 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3989 5373 5641 1632 Size: 2357 1384 268 1917 Fragments arranged by size: 2357 1917 1384 268 AfeI AGC'GCT Cuts at: 2298 2298 Size: 5926 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 4825 5802 1203 Size: 3622 977 1327 Fragments arranged by size: 3622 1327 977 AgeI A'CCGG_T Cuts at: 3039 4757 3039 Size: 1718 4208 AhdI GACnn_n'nnGTC Cuts at: 4655 4655 Size: 5926 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5926 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5926 AlwNI CAG_nnn'CTG Cuts at: 3669 5241 3669 Size: 1572 4354 ApaLI G'TGCA_C Cuts at: 3983 5139 3983 Size: 1156 4770 ApoI r'AATT_y Cuts at: 1493 3235 3461 3613 5722 1493 Size: 1742 226 152 2109 1697 Fragments arranged by size: 2109 1742 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4480 160 Size: 872 3448 1606 Fragments arranged by size: 3448 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 2525 2879 3251 1870 Size: 73 582 354 372 4545 Fragments arranged by size: 4545 582 372 354 73 BamHI G'GATC_C Cuts at: 3256 3623 3256 Size: 367 5559 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2313 2655 2765 3247 4702 Size: 325 217 627 526 342 110 482 1455 Cuts at: 4702 618 Size: 1842 Fragments arranged by size: 1842 1455 627 526 482 342 325 217 110 BanII G_rGCy'C Cuts at: 1391 2076 2880 3245 1391 Size: 685 804 365 4072 Fragments arranged by size: 4072 804 685 365 BbeI G_GCGC'C Cuts at: 2659 2659 Size: 5926 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2055 928 Size: 971 156 4799 Fragments arranged by size: 4799 971 156 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2860 2894 4117 4151 1903 Size: 34 923 34 1223 34 3678 Fragments arranged by size: 3678 1223 923 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3818 5028 3818 Size: 1210 4716 BclI T'GATC_A Cuts at: 1548 1548 Size: 5926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2774 2855 4537 Size: 107 122 71 631 605 1102 81 1682 Cuts at: 4537 136 Size: 1525 Fragments arranged by size: 1682 1525 1102 631 605 122 107 81 71 BglII A'GATC_T Cuts at: 2988 5921 2988 Size: 2933 2993 BlpI GC'TnA_GC Cuts at: 3343 3343 Size: 5926 Bme1580I G_kGCm'C Cuts at: 1790 2556 2770 3987 5143 1790 Size: 766 214 1217 1156 2573 Fragments arranged by size: 2573 1217 1156 766 214 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5926 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2618 2965 3657 Size: 416 53 83 399 24 1201 347 692 Cuts at: 3657 4615 442 Size: 958 1753 Fragments arranged by size: 1753 1201 958 692 416 399 347 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3173 1056 Size: 2117 3809 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4586 1239 Size: 3347 2579 Bpu10I CC'TnA_GC Cuts at: 2085 2085 Size: 5926 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4051 4916 5214 4051 Size: 865 298 4763 Fragments arranged by size: 4763 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4589 882 Size: 617 3090 2219 Fragments arranged by size: 3090 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2887 3717 493 Size: 711 1683 830 2702 Fragments arranged by size: 2702 1683 830 711 BsaBI GATnn'nnATC Cuts at: 5920 5920 Size: 5926 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2656 2787 3733 4115 Size: 53 83 186 936 1123 131 946 382 Cuts at: 4115 4750 275 Size: 635 1451 Fragments arranged by size: 1451 1123 946 936 635 382 186 131 83 53 BsaWI w'CCGG_w Cuts at: 3039 4359 4757 5031 5178 5589 3039 Size: 1320 398 274 147 411 3376 Fragments arranged by size: 3376 1320 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5701 Fragments arranged by size: 5701 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2519 1186 Size: 1333 4593 BseYI C'CCAG_C Cuts at: 2396 2492 2815 5129 5625 2396 Size: 96 323 2314 496 2697 Fragments arranged by size: 2697 2314 496 323 96 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5353 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2273 2617 3376 4137 5165 1540 Size: 275 186 272 344 759 761 1028 2301 Fragments arranged by size: 2301 1028 761 759 344 275 272 186 BsiHKAI G_wGCw'C Cuts at: 3987 4072 5143 3987 Size: 85 1071 4770 Fragments arranged by size: 4770 1071 85 BsmI GAATG_Cn' Cuts at: 3437 3530 3437 Size: 93 5833 BsmBI CGTCTCn'nnnn_ Cuts at: 2658 2658 Size: 5926 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2556 2770 2880 3245 3987 4072 Size: 399 286 480 214 110 365 742 85 Cuts at: 4072 5143 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 480 399 365 286 214 110 85 BspEI T'CCGG_A Cuts at: 5589 5589 Size: 5926 BspHI T'CATG_A Cuts at: 3815 5545 3815 Size: 1730 4196 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2130 3281 844 Size: 1286 1151 3489 Fragments arranged by size: 3489 1286 1151 BsrBI CCG'CTC Cuts at: 1394 1823 2440 3142 3813 1394 Size: 429 617 702 671 3507 Fragments arranged by size: 3507 702 671 617 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4421 4595 65 Size: 1183 3173 174 1396 Fragments arranged by size: 3173 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3003 3039 3063 4570 4757 1700 Size: 110 1193 36 24 1507 187 2869 Fragments arranged by size: 2869 1507 1193 187 110 36 24 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5926 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 3077 3986 4998 3077 Size: 909 1012 4005 Fragments arranged by size: 4005 1012 909 BstAPI GCAn_nnn'nTGC Cuts at: 794 2457 794 Size: 1663 4263 BstBI TT'CG_AA Cuts at: 4730 4730 Size: 5926 BstEII G'GTnAC_C Cuts at: 1184 2302 1184 Size: 1118 4808 BstXI CCAn_nnnn'nTGG Cuts at: 2851 2915 2851 Size: 64 5862 BstYI r'GATC_y Cuts at: 1972 2988 3256 3623 4008 4025 5477 5563 5575 Size: 1016 268 367 385 17 1452 86 12 Cuts at: 5575 5921 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1016 385 367 346 268 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2346 2997 2346 Size: 651 5275 BtgI C'CryG_G Cuts at: 513 1065 2004 2710 2850 2908 3009 513 Size: 552 939 706 140 58 101 3430 Fragments arranged by size: 3430 939 706 552 140 101 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2928 487 Size: 683 56 1702 3485 Fragments arranged by size: 3485 1702 683 56 BtsI GCAGTG_nn' Cuts at: 2217 2476 2482 2800 3442 4236 4256 2217 Size: 259 6 318 642 794 20 3887 Fragments arranged by size: 3887 794 642 318 259 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2632 2667 529 Size: 35 2068 35 3788 Fragments arranged by size: 3788 2068 35 35 DraI TTT'AAA Cuts at: 3230 3230 Size: 5926 DraIII CAC_nnn'GTG Cuts at: 3082 3082 Size: 5926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4933 809 Size: 507 3617 1802 Fragments arranged by size: 3617 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2092 2270 2625 Size: 54 1425 50 275 18 262 178 355 Cuts at: 2625 3373 4262 5891 8 Size: 748 889 1629 43 Fragments arranged by size: 1629 1425 889 748 355 275 262 178 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2270 3373 1537 Size: 275 458 1103 4090 Fragments arranged by size: 4090 1103 458 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2235 3856 1353 Size: 882 1621 3423 Fragments arranged by size: 3423 1621 882 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2786 3082 4513 4887 5033 387 Size: 1064 138 1197 296 1431 374 146 1280 Fragments arranged by size: 1431 1280 1197 1064 374 296 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3989 4586 5373 5641 1239 Size: 393 2357 597 787 268 1524 Fragments arranged by size: 2357 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2036 2413 2036 Size: 377 5549 EcoO109I rG'GnC_Cy Cuts at: 2190 2350 2638 2190 Size: 160 288 5478 Fragments arranged by size: 5478 288 160 EcoRI G'AATT_C Cuts at: 3235 3613 3235 Size: 378 5548 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2608 2608 Size: 5926 FseI GG_CCGG'CC Cuts at: 3007 3007 Size: 5926 FspI TGC'GCA Cuts at: 2806 4432 2806 Size: 1626 4300 HaeII r_GCGC'y Cuts at: 1678 2292 2300 2513 2533 2659 2923 5073 1678 Size: 614 8 213 20 126 264 2150 2531 Fragments arranged by size: 2531 2150 614 264 213 126 20 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4567 4599 4641 4673 1373 Size: 32 135 32 2995 32 42 32 2626 Fragments arranged by size: 2995 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2899 3270 3516 669 Size: 988 281 961 371 246 3079 Fragments arranged by size: 3079 988 961 371 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5926 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KasI G'GCGC_C Cuts at: 2655 2655 Size: 5926 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 3525 3525 Size: 5926 MluI A'CGCG_T Cuts at: 5802 5802 Size: 5926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5040 5224 1305 Size: 3735 184 2007 Fragments arranged by size: 3735 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4104 Fragments arranged by size: 4104 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2913 3884 4243 4402 518 Size: 648 1747 971 359 159 2042 Fragments arranged by size: 2042 1747 971 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2275 2463 3342 4019 5167 5412 1606 Size: 669 188 879 677 1148 245 2120 Fragments arranged by size: 2120 1148 879 677 669 245 188 NaeI GCC'GGC Cuts at: 3005 3005 Size: 5926 NarI GG'CG_CC Cuts at: 2656 2656 Size: 5926 NcoI C'CATG_G Cuts at: 513 1065 2004 2850 513 Size: 552 939 846 3589 Fragments arranged by size: 3589 939 846 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5926 NgoMIV G'CCGG_C Cuts at: 3003 3003 Size: 5926 NheI G'CTAG_C Cuts at: 1052 3169 1052 Size: 2117 3809 NotI GC'GGCC_GC Cuts at: 2270 3373 2270 Size: 1103 4823 NspI r_CATG'y Cuts at: 1132 1229 2563 3284 4829 1132 Size: 97 1334 721 1545 2229 Fragments arranged by size: 2229 1545 1334 721 97 PasI CC'CwG_GG Cuts at: 1348 2332 2409 1348 Size: 984 77 4865 Fragments arranged by size: 4865 984 77 PciI A'CATG_T Cuts at: 4825 4825 Size: 5926 PflMI CCAn_nnn'nTGG Cuts at: 1108 2323 1108 Size: 1215 4711 PfoI T'CCnGG_A Cuts at: 2155 2155 Size: 5926 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3995 4027 5508 5540 3995 Size: 32 1481 32 4381 Fragments arranged by size: 4381 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2190 2190 Size: 5926 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5926 PsiI TTA'TAA Cuts at: 3496 3496 Size: 5926 PstI C_TGCA'G Cuts at: 830 2280 2296 2462 2468 2988 3278 830 Size: 1450 16 166 6 520 290 3478 Fragments arranged by size: 3478 1450 520 290 166 16 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 PvuII CAG'CTG Cuts at: 2463 2463 Size: 5926 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SanDI GG'GwC_CC Cuts at: 2190 2190 Size: 5926 SbfI CC_TGCA'GG Cuts at: 3278 3278 Size: 5926 ScaI AGT'ACT Cuts at: 4174 5886 4174 Size: 1712 4214 SexAI A'CCwGG_T Cuts at: 3101 3101 Size: 5926 SfcI C'TryA_G Cuts at: 826 1045 2276 2292 2458 2464 2984 3274 4409 Size: 219 1231 16 166 6 520 290 1135 Cuts at: 4409 5090 5281 826 Size: 681 191 1471 Fragments arranged by size: 1471 1231 1135 681 520 290 219 191 166 16 6 SfoI GGC'GCC Cuts at: 2657 2657 Size: 5926 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5926 SmaI CCC'GGG Cuts at: 1872 3253 1872 Size: 1381 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4030 4931 5193 820 Size: 197 926 2087 901 262 1553 Fragments arranged by size: 2087 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2887 3717 493 Size: 2394 830 2702 Fragments arranged by size: 2702 2394 830 SpeI A'CTAG_T Cuts at: 152 152 Size: 5926 SphI G_CATG'C Cuts at: 1132 2563 3284 1132 Size: 1431 721 3774 Fragments arranged by size: 3774 1431 721 SspI AAT'ATT Cuts at: 5 52 3850 5 Size: 47 3798 2081 Fragments arranged by size: 3798 2081 47 StuI AGG'CCT Cuts at: 1517 2174 1517 Size: 657 5269 StyI C'CwwG_G Cuts at: 513 1065 2004 2076 2307 2844 2850 3135 3365 Size: 552 939 72 231 537 6 285 230 Cuts at: 3365 513 Size: 3074 Fragments arranged by size: 3074 939 552 537 285 231 230 72 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2410 2719 3953 3970 4123 4308 4753 Size: 1255 830 309 1234 17 153 185 445 Cuts at: 4753 325 Size: 1498 Fragments arranged by size: 1498 1255 1234 830 445 309 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4172 5884 96 Size: 274 80 33 51 1443 2195 1712 138 Fragments arranged by size: 2195 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 5886 5918 2000 Size: 32 3854 32 2008 Fragments arranged by size: 3854 2008 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2789 1316 Size: 1473 4453 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2107 2851 2107 Size: 744 5182 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5926 XmaI C'CCGG_G Cuts at: 1870 3251 1870 Size: 1381 4545 XmnI GAAnn'nnTTC Cuts at: 4055 4055 Size: 5926 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I EcoICRI EcoRV FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SrfI SwaI