(Circular) (Six-base) MAPSORT of: pFN21AB0918.seq Check: 733 from: 1 to: 5698 pFN21AB0918 5698 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3508 278 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3041 1937 Size: 1104 4594 Acc65I G'GTAC_C Cuts at: 3019 3019 Size: 5698 AclI AA'CG_TT Cuts at: 1563 3825 4198 1563 Size: 2262 373 3063 Fragments arranged by size: 3063 2262 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2214 2748 2897 3761 5145 5413 1632 Size: 582 534 149 864 1384 268 1917 Fragments arranged by size: 1917 1384 864 582 534 268 149 AflII C'TTAA_G Cuts at: 820 1017 2666 820 Size: 197 1649 3852 Fragments arranged by size: 3852 1649 197 AflIII A'CryG_T Cuts at: 1203 2135 4597 5574 1203 Size: 932 2462 977 1327 Fragments arranged by size: 2462 1327 977 932 AgeI A'CCGG_T Cuts at: 2096 4529 2096 Size: 2433 3265 AhdI GACnn_n'nnGTC Cuts at: 4427 4427 Size: 5698 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5698 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5698 AlwNI CAG_nnn'CTG Cuts at: 3441 5013 3441 Size: 1572 4126 ApaLI G'TGCA_C Cuts at: 2139 2793 3755 4911 2139 Size: 654 962 1156 2926 Fragments arranged by size: 2926 1156 962 654 ApoI r'AATT_y Cuts at: 1493 2336 2454 2513 2833 2859 3007 3233 3385 Size: 843 118 59 320 26 148 226 152 Cuts at: 3385 5494 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 843 320 226 152 148 118 59 26 AseI AT'TA_AT Cuts at: 160 1032 4252 160 Size: 872 3220 1606 Fragments arranged by size: 3220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 2030 3023 1870 Size: 73 87 993 4545 Fragments arranged by size: 4545 993 87 73 BamHI G'GATC_C Cuts at: 2257 3028 3395 2257 Size: 771 367 4560 Fragments arranged by size: 4560 771 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 3019 4474 618 Size: 325 217 627 258 974 1455 1842 Fragments arranged by size: 1842 1455 974 627 325 258 217 BanII G_rGCy'C Cuts at: 1391 3017 1391 Size: 1626 4072 BbeI G_GCGC'C Cuts at: 2049 2049 Size: 5698 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2826 928 Size: 971 927 3800 Fragments arranged by size: 3800 971 927 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 1903 Size: 34 1952 34 3678 Fragments arranged by size: 3678 1952 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3590 4800 3590 Size: 1210 4488 BclI T'GATC_A Cuts at: 1548 1548 Size: 5698 BfrBI ATG'CAT Cuts at: 2867 2867 Size: 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2026 4309 136 Size: 107 122 71 631 605 354 2283 1525 Fragments arranged by size: 2283 1525 631 605 354 122 107 71 BglII A'GATC_T Cuts at: 2956 5693 2956 Size: 2737 2961 BlpI GC'TnA_GC Cuts at: 3115 3115 Size: 5698 Bme1580I G_kGCm'C Cuts at: 1790 2143 2797 3759 4915 1790 Size: 353 654 962 1156 2573 Fragments arranged by size: 2573 1156 962 654 353 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5698 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3429 4387 442 Size: 416 53 83 399 24 2012 958 1753 Fragments arranged by size: 2012 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4358 1239 Size: 3119 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3823 4688 4986 3823 Size: 865 298 4535 Fragments arranged by size: 4535 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4361 882 Size: 617 2862 2219 Fragments arranged by size: 2862 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3489 493 Size: 711 2285 2702 Fragments arranged by size: 2702 2285 711 BsaBI GATnn'nnATC Cuts at: 2256 5692 2256 Size: 3436 2262 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 2046 3505 3887 Size: 53 83 186 936 482 31 1459 382 Cuts at: 3887 4522 275 Size: 635 1451 Fragments arranged by size: 1459 1451 936 635 482 382 186 83 53 31 BsaWI w'CCGG_w Cuts at: 2096 4131 4529 4803 4950 5361 2096 Size: 2035 398 274 147 411 2433 Fragments arranged by size: 2433 2035 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2346 2376 1178 Size: 30 165 30 943 30 4500 Fragments arranged by size: 4500 943 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2367 1186 Size: 889 292 4517 Fragments arranged by size: 4517 889 292 BseYI C'CCAG_C Cuts at: 4901 5397 4901 Size: 496 5202 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5125 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3148 3909 4937 1540 Size: 275 186 1147 761 1028 2301 Fragments arranged by size: 2301 1147 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2043 2143 2797 2939 3759 3844 4915 2043 Size: 100 654 142 820 85 1071 2826 Fragments arranged by size: 2826 1071 820 654 142 100 85 BsmI GAATG_Cn' Cuts at: 3209 3302 3209 Size: 93 5605 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2143 2797 2939 3017 3759 3844 Size: 399 253 100 654 142 78 742 85 Cuts at: 3844 4915 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 654 399 253 142 100 85 78 BspEI T'CCGG_A Cuts at: 5361 5361 Size: 5698 BspHI T'CATG_A Cuts at: 2206 3587 5317 2206 Size: 1381 1730 2587 Fragments arranged by size: 2587 1730 1381 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3053 844 Size: 2209 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3585 1394 Size: 429 1762 3507 Fragments arranged by size: 3507 1762 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4193 4367 65 Size: 1183 2945 174 1396 Fragments arranged by size: 2945 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2096 4342 4529 1700 Size: 110 286 2246 187 2869 Fragments arranged by size: 2869 2246 286 187 110 BsrGI T'GTAC_A Cuts at: 96 2569 96 Size: 2473 3225 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5698 BssSI C'ACGA_G Cuts at: 3758 4770 3758 Size: 1012 4686 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5698 BstBI TT'CG_AA Cuts at: 2817 4502 2817 Size: 1685 4013 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5698 BstYI r'GATC_y Cuts at: 1972 2257 2956 3028 3395 3780 3797 5249 5335 Size: 285 699 72 367 385 17 1452 86 Cuts at: 5335 5347 5693 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 699 385 367 346 285 86 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2361 513 Size: 552 939 357 3850 Fragments arranged by size: 3850 939 552 357 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2482 487 Size: 683 56 1256 3703 Fragments arranged by size: 3703 1256 683 56 BtsI GCAGTG_nn' Cuts at: 3214 4008 4028 3214 Size: 794 20 4884 Fragments arranged by size: 4884 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2861 2896 529 Size: 35 2297 35 3331 Fragments arranged by size: 3331 2297 35 35 DraI TTT'AAA Cuts at: 2376 3002 2376 Size: 626 5072 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4705 809 Size: 507 3389 1802 Fragments arranged by size: 3389 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3145 4034 5663 Size: 54 1425 50 275 18 1315 889 1629 Cuts at: 5663 8 Size: 43 Fragments arranged by size: 1629 1425 1315 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3145 1537 Size: 275 1333 4090 Fragments arranged by size: 4090 1333 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2616 2892 3628 1353 Size: 1263 276 736 3423 Fragments arranged by size: 3423 1263 736 276 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4285 4659 4805 387 Size: 1064 138 2696 374 146 1280 Fragments arranged by size: 2696 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2214 2748 2897 3761 4358 5145 5413 Size: 393 582 534 149 864 597 787 268 Cuts at: 5413 1239 Size: 1524 Fragments arranged by size: 1524 864 787 597 582 534 393 268 149 EcoRI G'AATT_C Cuts at: 3007 3385 3007 Size: 378 5320 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2705 2705 Size: 5698 FspI TGC'GCA Cuts at: 4204 4204 Size: 5698 HaeII r_GCGC'y Cuts at: 1678 2049 2084 4845 1678 Size: 371 35 2761 2531 Fragments arranged by size: 2761 2531 371 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2276 2308 2485 2517 2948 Size: 32 135 32 704 32 177 32 431 Cuts at: 2948 2980 4339 4371 4413 4445 1373 Size: 32 1359 32 42 32 2626 Fragments arranged by size: 2626 1359 704 431 177 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3042 3288 669 Size: 988 281 1104 246 3079 Fragments arranged by size: 3079 1104 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5698 HpaI GTT'AAC Cuts at: 3288 3288 Size: 5698 KasI G'GCGC_C Cuts at: 2045 2045 Size: 5698 KpnI G_GTAC'C Cuts at: 3023 3023 Size: 5698 MfeI C'AATT_G Cuts at: 3297 3297 Size: 5698 MluI A'CGCG_T Cuts at: 5574 5574 Size: 5698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4812 4996 1305 Size: 3507 184 2007 Fragments arranged by size: 3507 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3876 Fragments arranged by size: 3876 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2640 3656 4015 4174 518 Size: 648 1474 1016 359 159 2042 Fragments arranged by size: 2042 1474 1016 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3114 3791 4939 5184 1606 Size: 1508 677 1148 245 2120 Fragments arranged by size: 2120 1508 1148 677 245 NarI GG'CG_CC Cuts at: 2046 2046 Size: 5698 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4207 Fragments arranged by size: 4207 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5698 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5698 NotI GC'GGCC_GC Cuts at: 3145 3145 Size: 5698 NsiI A_TGCA'T Cuts at: 2869 2869 Size: 5698 NspI r_CATG'y Cuts at: 1132 1229 2139 2483 3056 4601 1132 Size: 97 910 344 573 1545 2229 Fragments arranged by size: 2229 1545 910 573 344 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5698 PciI A'CATG_T Cuts at: 2135 4597 2135 Size: 2462 3236 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5698 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3767 3799 5280 5312 3767 Size: 32 1481 32 4153 Fragments arranged by size: 4153 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5698 PsiI TTA'TAA Cuts at: 2460 3268 2460 Size: 808 4890 PstI C_TGCA'G Cuts at: 830 3050 830 Size: 2220 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SapI GCTCTTCn'nnn_ Cuts at: 2616 2616 Size: 5698 SbfI CC_TGCA'GG Cuts at: 3050 3050 Size: 5698 ScaI AGT'ACT Cuts at: 3946 5658 3946 Size: 1712 3986 SfcI C'TryA_G Cuts at: 826 1045 2806 3046 4181 4862 5053 826 Size: 219 1761 240 1135 681 191 1471 Fragments arranged by size: 1761 1471 1135 681 240 219 191 SfoI GGC'GCC Cuts at: 2047 2047 Size: 5698 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 2096 1700 Size: 396 5302 SmaI CCC'GGG Cuts at: 1872 3025 1872 Size: 1153 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2666 3802 4703 4965 820 Size: 197 926 723 1136 901 262 1553 Fragments arranged by size: 1553 1136 926 901 723 262 197 SnaBI TAC'GTA Cuts at: 493 3489 493 Size: 2996 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 3056 1132 Size: 1924 3774 SspI AAT'ATT Cuts at: 5 52 2715 3622 5 Size: 47 2663 907 2081 Fragments arranged by size: 2663 2081 907 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5698 StyI C'CwwG_G Cuts at: 513 1065 2004 3137 513 Size: 552 939 1133 3074 Fragments arranged by size: 3074 1133 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3725 3742 3895 4080 4525 325 Size: 1255 2145 17 153 185 445 1498 Fragments arranged by size: 2145 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2569 3944 5656 Size: 274 80 33 51 1443 592 1375 1712 Cuts at: 5656 96 Size: 138 Fragments arranged by size: 1712 1443 1375 592 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2346 2378 5658 5690 2346 Size: 32 3280 32 2354 Fragments arranged by size: 3280 2354 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5698 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 3023 1870 Size: 1153 4545 XmnI GAAnn'nnTTC Cuts at: 3827 3827 Size: 5698 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3506 276 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI