Restriction map for pFN21AB0918
 (Circular) (Six-base) MAPSORT of: pFN21AB0918.seq  Check: 733  from: 1  to: 5698



pFN21AB0918  5698 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:33  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3508     278 
   Size:         53      83     186     936    1972    2468

  Fragments arranged by size: 

                2468    1972     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3041    1937 
   Size:       1104    4594
 
Acc65I G'GTAC_C

Cuts at:   3019    3019 
   Size:       5698
 
AclI AA'CG_TT

Cuts at:   1563    3825    4198    1563 
   Size:       2262     373    3063

  Fragments arranged by size: 

                3063    2262     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2214    2748    2897    3761    5145    5413    1632 
   Size:        582     534     149     864    1384     268    1917

  Fragments arranged by size: 

                1917    1384     864     582     534     268     149
 
AflII C'TTAA_G

Cuts at:    820    1017    2666     820 
   Size:        197    1649    3852

  Fragments arranged by size: 

                3852    1649     197
 
AflIII A'CryG_T

Cuts at:   1203    2135    4597    5574    1203 
   Size:        932    2462     977    1327

  Fragments arranged by size: 

                2462    1327     977     932
 
AgeI A'CCGG_T

Cuts at:   2096    4529    2096 
   Size:       2433    3265
 
AhdI GACnn_n'nnGTC

Cuts at:   4427    4427 
   Size:       5698
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5698
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5698
 
AlwNI CAG_nnn'CTG

Cuts at:   3441    5013    3441 
   Size:       1572    4126
 
ApaLI G'TGCA_C

Cuts at:   2139    2793    3755    4911    2139 
   Size:        654     962    1156    2926

  Fragments arranged by size: 

                2926    1156     962     654
 
ApoI r'AATT_y

Cuts at:   1493    2336    2454    2513    2833    2859    3007    3233    3385 
   Size:        843     118      59     320      26     148     226     152

Cuts at:   3385    5494    1493 
   Size:       2109    1697

  Fragments arranged by size: 

                2109    1697     843     320     226     152     148     118
                  59      26
 
AseI AT'TA_AT

Cuts at:    160    1032    4252     160 
   Size:        872    3220    1606

  Fragments arranged by size: 

                3220    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5698
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2030    3023    1870 
   Size:         73      87     993    4545

  Fragments arranged by size: 

                4545     993      87      73
 
BamHI G'GATC_C

Cuts at:   2257    3028    3395    2257 
   Size:        771     367    4560

  Fragments arranged by size: 

                4560     771     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2045    3019    4474     618 
   Size:        325     217     627     258     974    1455    1842

  Fragments arranged by size: 

                1842    1455     974     627     325     258     217
 
BanII G_rGCy'C

Cuts at:   1391    3017    1391 
   Size:       1626    4072
 
BbeI G_GCGC'C

Cuts at:   2049    2049 
   Size:       5698
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2826     928 
   Size:        971     927    3800

  Fragments arranged by size: 

                3800     971     927
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3889    3923    1903 
   Size:         34    1952      34    3678

  Fragments arranged by size: 

                3678    1952      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3590    4800    3590 
   Size:       1210    4488
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5698
 
BfrBI ATG'CAT

Cuts at:   2867    2867 
   Size:       5698
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2026    4309     136 
   Size:        107     122      71     631     605     354    2283    1525

  Fragments arranged by size: 

                2283    1525     631     605     354     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2956    5693    2956 
   Size:       2737    2961
 
BlpI GC'TnA_GC

Cuts at:   3115    3115 
   Size:       5698
 
Bme1580I G_kGCm'C

Cuts at:   1790    2143    2797    3759    4915    1790 
   Size:        353     654     962    1156    2573

  Fragments arranged by size: 

                2573    1156     962     654     353
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5698
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3429    4387     442 
   Size:        416      53      83     399      24    2012     958    1753

  Fragments arranged by size: 

                2012    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5698
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4358    1239 
   Size:       3119    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3823    4688    4986    3823 
   Size:        865     298    4535

  Fragments arranged by size: 

                4535     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4361     882 
   Size:        617    2862    2219

  Fragments arranged by size: 

                2862    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3489     493 
   Size:        711    2285    2702

  Fragments arranged by size: 

                2702    2285     711
 
BsaBI GATnn'nnATC

Cuts at:   2256    5692    2256 
   Size:       3436    2262
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2015    2046    3505    3887 
   Size:         53      83     186     936     482      31    1459     382

Cuts at:   3887    4522     275 
   Size:        635    1451

  Fragments arranged by size: 

                1459    1451     936     635     482     382     186      83
                  53      31
 
BsaWI w'CCGG_w

Cuts at:   2096    4131    4529    4803    4950    5361    2096 
   Size:       2035     398     274     147     411    2433

  Fragments arranged by size: 

                2433    2035     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2346    2376    1178 
   Size:         30     165      30     943      30    4500

  Fragments arranged by size: 

                4500     943     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2075    2367    1186 
   Size:        889     292    4517

  Fragments arranged by size: 

                4517     889     292
 
BseYI C'CCAG_C

Cuts at:   4901    5397    4901 
   Size:        496    5202
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5125
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3148    3909    4937    1540 
   Size:        275     186    1147     761    1028    2301

  Fragments arranged by size: 

                2301    1147    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2043    2143    2797    2939    3759    3844    4915    2043 
   Size:        100     654     142     820      85    1071    2826

  Fragments arranged by size: 

                2826    1071     820     654     142     100      85
 
BsmI GAATG_Cn'

Cuts at:   3209    3302    3209 
   Size:         93    5605
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2043    2143    2797    2939    3017    3759    3844 
   Size:        399     253     100     654     142      78     742      85

Cuts at:   3844    4915    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     654     399     253     142     100
                  85      78
 
BspEI T'CCGG_A

Cuts at:   5361    5361 
   Size:       5698
 
BspHI T'CATG_A

Cuts at:   2206    3587    5317    2206 
   Size:       1381    1730    2587

  Fragments arranged by size: 

                2587    1730    1381
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3053     844 
   Size:       2209    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3585    1394 
   Size:        429    1762    3507

  Fragments arranged by size: 

                3507    1762     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4193    4367      65 
   Size:       1183    2945     174    1396

  Fragments arranged by size: 

                2945    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2096    4342    4529    1700 
   Size:        110     286    2246     187    2869

  Fragments arranged by size: 

                2869    2246     286     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2569      96 
   Size:       2473    3225
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5698
 
BssSI C'ACGA_G

Cuts at:   3758    4770    3758 
   Size:       1012    4686
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5698
 
BstBI TT'CG_AA

Cuts at:   2817    4502    2817 
   Size:       1685    4013
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5698
 
BstYI r'GATC_y

Cuts at:   1972    2257    2956    3028    3395    3780    3797    5249    5335 
   Size:        285     699      72     367     385      17    1452      86

Cuts at:   5335    5347    5693    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     699     385     367     346     285      86
                  72      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2361     513 
   Size:        552     939     357    3850

  Fragments arranged by size: 

                3850     939     552     357
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2482     487 
   Size:        683      56    1256    3703

  Fragments arranged by size: 

                3703    1256     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3214    4008    4028    3214 
   Size:        794      20    4884

  Fragments arranged by size: 

                4884     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5698
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2861    2896     529 
   Size:         35    2297      35    3331

  Fragments arranged by size: 

                3331    2297      35      35
 
DraI TTT'AAA

Cuts at:   2376    3002    2376 
   Size:        626    5072
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4705     809 
   Size:        507    3389    1802

  Fragments arranged by size: 

                3389    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3145    4034    5663 
   Size:         54    1425      50     275      18    1315     889    1629

Cuts at:   5663       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1315     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3145    1537 
   Size:        275    1333    4090

  Fragments arranged by size: 

                4090    1333     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2616    2892    3628    1353 
   Size:       1263     276     736    3423

  Fragments arranged by size: 

                3423    1263     736     276
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4285    4659    4805     387 
   Size:       1064     138    2696     374     146    1280

  Fragments arranged by size: 

                2696    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2214    2748    2897    3761    4358    5145    5413 
   Size:        393     582     534     149     864     597     787     268

Cuts at:   5413    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     864     787     597     582     534     393     268
                 149
 
EcoRI G'AATT_C

Cuts at:   3007    3385    3007 
   Size:        378    5320
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2705    2705 
   Size:       5698
 
FspI TGC'GCA

Cuts at:   4204    4204 
   Size:       5698
 
HaeII r_GCGC'y

Cuts at:   1678    2049    2084    4845    1678 
   Size:        371      35    2761    2531

  Fragments arranged by size: 

                2761    2531     371      35
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2276    2308    2485    2517    2948 
   Size:         32     135      32     704      32     177      32     431

Cuts at:   2948    2980    4339    4371    4413    4445    1373 
   Size:         32    1359      32      42      32    2626

  Fragments arranged by size: 

                2626    1359     704     431     177     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3042    3288     669 
   Size:        988     281    1104     246    3079

  Fragments arranged by size: 

                3079    1104     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5698
 
HpaI GTT'AAC

Cuts at:   3288    3288 
   Size:       5698
 
KasI G'GCGC_C

Cuts at:   2045    2045 
   Size:       5698
 
KpnI G_GTAC'C

Cuts at:   3023    3023 
   Size:       5698
 
MfeI C'AATT_G

Cuts at:   3297    3297 
   Size:       5698
 
MluI A'CGCG_T

Cuts at:   5574    5574 
   Size:       5698
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4812    4996    1305 
   Size:       3507     184    2007

  Fragments arranged by size: 

                3507    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3876

  Fragments arranged by size: 

                3876    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2640    3656    4015    4174     518 
   Size:        648    1474    1016     359     159    2042

  Fragments arranged by size: 

                2042    1474    1016     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3114    3791    4939    5184    1606 
   Size:       1508     677    1148     245    2120

  Fragments arranged by size: 

                2120    1508    1148     677     245
 
NarI GG'CG_CC

Cuts at:   2046    2046 
   Size:       5698
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4207

  Fragments arranged by size: 

                4207     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5698
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5698
 
NotI GC'GGCC_GC

Cuts at:   3145    3145 
   Size:       5698
 
NsiI A_TGCA'T

Cuts at:   2869    2869 
   Size:       5698
 
NspI r_CATG'y

Cuts at:   1132    1229    2139    2483    3056    4601    1132 
   Size:         97     910     344     573    1545    2229

  Fragments arranged by size: 

                2229    1545     910     573     344      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5698
 
PciI A'CATG_T

Cuts at:   2135    4597    2135 
   Size:       2462    3236
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5698
 
PmeI GTTT'AAAC

Cuts at:   3002    3002 
   Size:       5698
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3767    3799    5280    5312    3767 
   Size:         32    1481      32    4153

  Fragments arranged by size: 

                4153    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5698
 
PsiI TTA'TAA

Cuts at:   2460    3268    2460 
   Size:        808    4890
 
PstI C_TGCA'G

Cuts at:    830    3050     830 
   Size:       2220    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5698
 
SalI G'TCGA_C

Cuts at:   1936    3040    1936 
   Size:       1104    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2616    2616 
   Size:       5698
 
SbfI CC_TGCA'GG

Cuts at:   3050    3050 
   Size:       5698
 
ScaI AGT'ACT

Cuts at:   3946    5658    3946 
   Size:       1712    3986
 
SfcI C'TryA_G

Cuts at:    826    1045    2806    3046    4181    4862    5053     826 
   Size:        219    1761     240    1135     681     191    1471

  Fragments arranged by size: 

                1761    1471    1135     681     240     219     191
 
SfoI GGC'GCC

Cuts at:   2047    2047 
   Size:       5698
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5698
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    2096    1700 
   Size:        396    5302
 
SmaI CCC'GGG

Cuts at:   1872    3025    1872 
   Size:       1153    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2666    3802    4703    4965     820 
   Size:        197     926     723    1136     901     262    1553

  Fragments arranged by size: 

                1553    1136     926     901     723     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3489     493 
   Size:       2996    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5698
 
SphI G_CATG'C

Cuts at:   1132    3056    1132 
   Size:       1924    3774
 
SspI AAT'ATT

Cuts at:      5      52    2715    3622       5 
   Size:         47    2663     907    2081

  Fragments arranged by size: 

                2663    2081     907      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5698
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3137     513 
   Size:        552     939    1133    3074

  Fragments arranged by size: 

                3074    1133     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3725    3742    3895    4080    4525     325 
   Size:       1255    2145      17     153     185     445    1498

  Fragments arranged by size: 

                2145    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2569    3944    5656 
   Size:        274      80      33      51    1443     592    1375    1712

Cuts at:   5656      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1375     592     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2346    2378    5658    5690    2346 
   Size:         32    3280      32    2354

  Fragments arranged by size: 

                3280    2354      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5698
 
XbaI T'CTAG_A

Cuts at:   3034    3034 
   Size:       5698
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5698
 
XmaI C'CCGG_G

Cuts at:   1870    3023    1870 
   Size:       1153    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3827    3827 
   Size:       5698
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3506     276 
   Size:         53      83     186     936    1972    2468

  Fragments arranged by size: 

                2468    1972     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NsiI     NspI
     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI
     PvuI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BbvCI
     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI EcoO109I    EcoRV     FseI    FspAI     NaeI   NgoMIV
     NruI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI
    PvuII    RsrII     SacI    SacII    SanDI    SexAI     SfiI     SrfI
     SwaI     XcmI
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