Restriction map for
pFN21AB0905
##################################
# NO CUT ENZYMES ( 185 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AluI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI MwoI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 134 CGCG CG^CG
AfaI 224 GTAC GT^AC
AlwI 129 GATCC GGATC(4/5)
AspHI 174 GTGCTC G[AT]GC[AT]^C
AvrII 51 CCTAGG C^CTAGG
BbsI 193 GTCTTC GAAGAC(2/6)
BcnI 184 CCCGG CC^[GC]GG
BfaI 52 CTAG C^TAG
BglII 5 AGATCT A^GATCT
BlnI 51 CCTAGG C^CTAGG
BmyI 174 GTGCTC G[AGT]GC[ACT]^C
BpuAI 193 GTCTTC GAAGAC(2/6)
BsaI 160 GAGACC GGTCTC(1/5)
BsiHKAI 174 GTGCTC G[AT]GC[AT]^C
BsiYI 71 CCTCCTCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 71 CCTCCTCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 160 GAGAC GTCTC(1/5)
Bsp1286I 174 GTGCTC G[AGT]GC[ACT]^C
BstNI 249 CCAGG CC^[AT]GG
BstUI 134 CGCG CG^CG
DdeI 96 CTCAG C^T[ACGT]AG
DraII 81 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 238 GACAAGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 11 CCGTGG C^C[GA][CT]GG
EcoO109I 81 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 249 CCAGG ^CC[AT]GG
EcoT14I 51 CCTAGG C^C[AT][AT]GG
FauI 132 CCCGC CCCGC(4/6)
HaeIII 211 GGCC GG^CC
HapII 185 CCGG C^CGG
HgaI 135 GCGTC GACGC(5/10)
HinfI 67 GAATC G^A[ACGT]TC
HpaII 185 CCGG C^CGG
MaeI 52 CTAG C^TAG
MaeIII 236 GTGAC ^GT[ACGT]AC
MspA1I 139 CAGCGG C[AC]G^C[GT]G
MspI 185 CCGG C^CGG
MvaI 249 CCAGG CC^[AT]GG
MvnI 134 CGCG CG^CG
NciI 184 CCCGG CC^[GC]GG
NlaIII 267 CATG CATG^
PpuMI 81 GGGACCT [GA]G^G[AT]CC[CT]
RsaI 224 GTAC GT^AC
StyI 51 CCTAGG C^C[AT][AT]GG
TfiI 67 GAATC G^A[AT]TC
Tsp45I 236 GTGAC ^GT[GC]AC
Tsp509I 189 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 133 CCGC CCGC(-3/-1)
141 GCGG
BbvI 16 GCTGC GCAGC(8/12)
104 GCTGC
BsaJI 11 CCGTGG C^C[ACGT][ACGT]GG
51 CCTAGG
BseRI 34 CTCCTC GAGGAG(10/8)
72 CTCCTC
BsmFI 55 GGGAC GGGAC(10/14)
81 GGGAC
BsrI 171 CCAGT ACTGG(1/-1)
233 CCAGT
BssKI 184 CCCGG ^CC[ACGT]GG
249 CCAGG
BstYI 5 AGATCT [GA]^GATC[CT]
128 AGATCC
DpnII 6 GATC ^GATC
129 GATC
Fnu4HI 16 GCTGC GC^[ACGT]GC
104 GCTGC
ItaI 16 GCTGC GC^[ACGT]GC
104 GCTGC
MboI 6 GATC ^GATC
129 GATC
MflI 5 AGATCT [GA]^GATC[CT]
128 AGATCC
NdeII 6 GATC ^GATC
129 GATC
Sau3AI 6 GATC ^GATC
129 GATC
ScrFI 184 CCCGG CC^[ACGT]GG
249 CCAGG
TseI 16 GCTGC G^C[AT]GC
104 GCTGC
TspRI 172 CAGTG CA[GC]TG[ACGT][ACGT]^
234 CAGTG
XhoII 5 AGATCT [GA]^GATC[CT]
128 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
AvaII Cfr13I HphI MboII MnlI
NlaIV Sau96I