(Circular) (Six-base) MAPSORT of: pFN21AB0902.seq Check: 552 from: 1 to: 5596 pFN21AB0902 5596 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2558 2558 Size: 5596 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2355 2445 2939 1937 Size: 418 90 494 4594 Fragments arranged by size: 4594 494 418 90 Acc65I G'GTAC_C Cuts at: 2917 2917 Size: 5596 AclI AA'CG_TT Cuts at: 1563 3723 4096 1563 Size: 2160 373 3063 Fragments arranged by size: 3063 2160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2030 2837 3659 5043 5311 1632 Size: 398 807 822 1384 268 1917 Fragments arranged by size: 1917 1384 822 807 398 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5399 AflIII A'CryG_T Cuts at: 1203 2576 4495 5472 1203 Size: 1373 1919 977 1327 Fragments arranged by size: 1919 1373 1327 977 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 2095 4325 2095 Size: 2230 3366 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5596 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5596 AlwNI CAG_nnn'CTG Cuts at: 3339 4911 3339 Size: 1572 4024 ApaLI G'TGCA_C Cuts at: 3653 4809 3653 Size: 1156 4440 ApoI r'AATT_y Cuts at: 1493 2905 3131 3283 5392 1493 Size: 1412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 226 152 AseI AT'TA_AT Cuts at: 160 1032 4150 160 Size: 872 3118 1606 Fragments arranged by size: 3118 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2921 1870 Size: 73 978 4545 Fragments arranged by size: 4545 978 73 AvrII C'CTAG_G Cuts at: 2410 2410 Size: 5596 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2917 4372 618 Size: 325 217 627 1130 1455 1842 Fragments arranged by size: 1842 1455 1130 627 325 217 BanII G_rGCy'C Cuts at: 1391 2915 1391 Size: 1524 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4625 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3787 3821 1903 Size: 34 1850 34 3678 Fragments arranged by size: 3678 1850 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2511 3488 4698 2511 Size: 977 1210 3409 Fragments arranged by size: 3409 1210 977 BclI T'GATC_A Cuts at: 1548 2671 1548 Size: 1123 4473 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4207 136 Size: 107 122 71 631 605 2535 1525 Fragments arranged by size: 2535 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5591 5591 Size: 5596 BlpI GC'TnA_GC Cuts at: 3013 3013 Size: 5596 Bme1580I G_kGCm'C Cuts at: 1790 2115 2642 3657 4813 1790 Size: 325 527 1015 1156 2573 Fragments arranged by size: 2573 1156 1015 527 325 BmgBI CAC'GTC Cuts at: 1318 2579 1318 Size: 1261 4335 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2551 3327 4285 Size: 416 53 83 399 24 1134 776 958 Cuts at: 4285 442 Size: 1753 Fragments arranged by size: 1753 1134 958 776 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4256 1239 Size: 3017 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2440 3721 4586 4884 2440 Size: 1281 865 298 3152 Fragments arranged by size: 3152 1281 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2416 4259 882 Size: 617 917 1843 2219 Fragments arranged by size: 2219 1843 917 617 BsaAI yAC'GTr Cuts at: 493 1204 3387 493 Size: 711 2183 2702 Fragments arranged by size: 2702 2183 711 BsaBI GATnn'nnATC Cuts at: 5590 5590 Size: 5596 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3403 3785 4420 275 Size: 53 83 186 936 1870 382 635 1451 Fragments arranged by size: 1870 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2661 4029 4427 4701 4848 5259 2661 Size: 1368 398 274 147 411 2998 Fragments arranged by size: 2998 1368 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5371 Fragments arranged by size: 5371 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 2192 2210 2213 2216 2387 2810 1186 Size: 991 15 18 3 3 171 423 3972 Fragments arranged by size: 3972 991 423 171 18 15 3 3 BseYI C'CCAG_C Cuts at: 2027 2139 4799 5295 2027 Size: 112 2660 496 2328 Fragments arranged by size: 2660 2328 496 112 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2825 1163 Size: 573 1089 3934 Fragments arranged by size: 3934 1089 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3046 3807 4835 1540 Size: 275 186 1045 761 1028 2301 Fragments arranged by size: 2301 1045 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3657 3742 4813 3657 Size: 85 1071 4440 Fragments arranged by size: 4440 1071 85 BsmI GAATG_Cn' Cuts at: 2278 2871 3107 3200 2278 Size: 593 236 93 4674 Fragments arranged by size: 4674 593 236 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2642 2915 3657 3742 4813 1391 Size: 399 325 527 273 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 527 399 325 273 85 BspEI T'CCGG_A Cuts at: 5259 5259 Size: 5596 BspHI T'CATG_A Cuts at: 3485 5215 3485 Size: 1730 3866 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2558 2951 844 Size: 1714 393 3489 Fragments arranged by size: 3489 1714 393 BsrBI CCG'CTC Cuts at: 1394 1823 3483 1394 Size: 429 1660 3507 Fragments arranged by size: 3507 1660 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4091 4265 65 Size: 1183 2843 174 1396 Fragments arranged by size: 2843 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4240 4427 1700 Size: 110 2430 187 2869 Fragments arranged by size: 2869 2430 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5596 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5596 BssSI C'ACGA_G Cuts at: 3656 4668 3656 Size: 1012 4584 BstAPI GCAn_nnn'nTGC Cuts at: 794 2782 794 Size: 1988 3608 BstBI TT'CG_AA Cuts at: 4400 4400 Size: 5596 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5596 BstXI CCAn_nnnn'nTGG Cuts at: 2074 2743 2074 Size: 669 4927 BstYI r'GATC_y Cuts at: 1972 2926 3293 3678 3695 5147 5233 5245 5591 Size: 954 367 385 17 1452 86 12 346 Cuts at: 5591 1972 Size: 1977 Fragments arranged by size: 1977 1452 954 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2226 513 Size: 552 939 222 3883 Fragments arranged by size: 3883 939 552 222 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4857 Fragments arranged by size: 4857 683 56 BtsI GCAGTG_nn' Cuts at: 3112 3906 3926 3112 Size: 794 20 4782 Fragments arranged by size: 4782 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2823 2858 529 Size: 35 2259 35 3267 Fragments arranged by size: 3267 2259 35 35 DraI TTT'AAA Cuts at: 2689 2900 2689 Size: 211 5385 DraIII CAC_nnn'GTG Cuts at: 2782 2782 Size: 5596 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4603 809 Size: 507 3287 1802 Fragments arranged by size: 3287 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2607 3043 3932 Size: 54 1425 50 275 18 777 436 889 Cuts at: 3932 5561 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 777 436 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3043 1537 Size: 275 1231 4090 Fragments arranged by size: 4090 1231 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2277 2382 2487 2717 3526 1353 Size: 924 105 105 230 809 3423 Fragments arranged by size: 3423 924 809 230 105 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4183 4557 4703 387 Size: 1064 138 2594 374 146 1280 Fragments arranged by size: 2594 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2837 3659 4256 5043 5311 1239 Size: 393 398 807 822 597 787 268 1524 Fragments arranged by size: 1524 822 807 787 597 398 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2048 2048 Size: 5596 EcoO109I rG'GnC_Cy Cuts at: 2089 2089 Size: 5596 EcoRI G'AATT_C Cuts at: 2905 3283 2905 Size: 378 5218 FspI TGC'GCA Cuts at: 4102 4102 Size: 5596 HaeII r_GCGC'y Cuts at: 1678 4743 1678 Size: 3065 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 2656 2688 4237 Size: 32 135 32 509 32 543 32 1549 Cuts at: 4237 4269 4311 4343 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1549 543 509 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2940 3186 669 Size: 988 281 1002 246 3079 Fragments arranged by size: 3079 1002 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5596 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KpnI G_GTAC'C Cuts at: 2921 2921 Size: 5596 MfeI C'AATT_G Cuts at: 3195 3195 Size: 5596 MluI A'CGCG_T Cuts at: 5472 5472 Size: 5596 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2808 4710 4894 1305 Size: 1503 1902 184 2007 Fragments arranged by size: 2007 1902 1503 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2609 10 Size: 54 1425 343 777 2997 Fragments arranged by size: 2997 1425 777 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2741 3554 3913 4072 518 Size: 648 1575 813 359 159 2042 Fragments arranged by size: 2042 1575 813 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2106 2143 2605 3012 3689 4837 5082 1606 Size: 500 37 462 407 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 500 462 407 245 37 NcoI C'CATG_G Cuts at: 513 1065 2004 2226 513 Size: 552 939 222 3883 Fragments arranged by size: 3883 939 552 222 NdeI CA'TA_TG Cuts at: 387 387 Size: 5596 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 3043 3043 Size: 5596 NspI r_CATG'y Cuts at: 1132 1229 2683 2954 4499 1132 Size: 97 1454 271 1545 2229 Fragments arranged by size: 2229 1545 1454 271 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5596 PciI A'CATG_T Cuts at: 4495 4495 Size: 5596 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5596 PfoI T'CCnGG_A Cuts at: 2759 2759 Size: 5596 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2304 2336 3665 3697 5178 5210 2304 Size: 32 1329 32 1481 32 2690 Fragments arranged by size: 2690 1481 1329 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2089 2089 Size: 5596 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5596 PsiI TTA'TAA Cuts at: 3166 3166 Size: 5596 PstI C_TGCA'G Cuts at: 830 2948 830 Size: 2118 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 PvuII CAG'CTG Cuts at: 2106 2143 2605 2106 Size: 37 462 5097 Fragments arranged by size: 5097 462 37 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SapI GCTCTTCn'nnn_ Cuts at: 2487 2487 Size: 5596 SbfI CC_TGCA'GG Cuts at: 2948 2948 Size: 5596 ScaI AGT'ACT Cuts at: 3844 5556 3844 Size: 1712 3884 SfcI C'TryA_G Cuts at: 826 1045 2944 4079 4760 4951 826 Size: 219 1899 1135 681 191 1471 Fragments arranged by size: 1899 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5596 SmaI CCC'GGG Cuts at: 1872 2923 1872 Size: 1051 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2419 3700 4601 4863 820 Size: 197 926 476 1281 901 262 1553 Fragments arranged by size: 1553 1281 926 901 476 262 197 SnaBI TAC'GTA Cuts at: 493 3387 493 Size: 2894 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5596 SphI G_CATG'C Cuts at: 1132 2954 1132 Size: 1822 3774 SspI AAT'ATT Cuts at: 5 52 3520 5 Size: 47 3468 2081 Fragments arranged by size: 3468 2081 47 StuI AGG'CCT Cuts at: 1517 2885 1517 Size: 1368 4228 StyI C'CwwG_G Cuts at: 513 1065 2004 2226 2410 3035 513 Size: 552 939 222 184 625 3074 Fragments arranged by size: 3074 939 625 552 222 184 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3623 3640 3793 3978 4423 325 Size: 1255 2043 17 153 185 445 1498 Fragments arranged by size: 2043 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3842 5554 96 Size: 274 80 33 51 1443 1865 1712 138 Fragments arranged by size: 1865 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2045 2077 5556 5588 2045 Size: 32 3479 32 2053 Fragments arranged by size: 3479 2053 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5596 XbaI T'CTAG_A Cuts at: 2932 2932 Size: 5596 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2602 2602 Size: 5596 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5596 XmaI C'CCGG_G Cuts at: 1870 2921 1870 Size: 1051 4545 XmnI GAAnn'nnTTC Cuts at: 2711 2871 3725 2711 Size: 160 854 4582 Fragments arranged by size: 4582 854 160 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI