Restriction map for pFN21AB0477
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvrII       BamHI       
BbeI        BbsI        BcgI        BciVI       BclI        
BfaI        BfrI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrDI       BsrGI       Bst1107I    BstAPI      
BstBI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        Hin1I       
HpaI        KasI        KpnI        MaeI        MfeI        
MluI        MseI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SapI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SspBI       SspI        SwaI        TfiI        
Tru9I       Tth111I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcsI          845  AAATTT               [GA]^AATT[CT]
AflIII        665  ACGTGT               A^C[GA][CT]GT
AgeI          677  ACCGGT               A^CCGGT
ApaI          353  GGGCCC               GGGCC^C
ApoI          845  AAATTT               [GA]^AATT[CT]
AviII         190  TGCGCA               TGC^GCA
BanI          777  GGTGCC               G^G[CT][GA]CC
BglI          355  GCCCTGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI        1125  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrBI         181  GAGCGG               CCGCTC(-3/-3)
BsrFI         677  ACCGGT               [GA]^CCGG[CT]
BssHII        142  GCGCGC               G^CGCGC
BssSI        1426  CTCGTG               CACGAG(-5/-1)
BstEII       1254  GGTCACC              G^GT[ACGT]ACC
BstPI        1254  GGTCACC              G^GT[ACGT]ACC
Cfr10I        677  ACCGGT               [GA]^CCGG[CT]
EcoO65I      1254  GGTCACC              G^GT[ACGT]ACC
FspI          190  TGCGCA               TGC^GCA
HgaI            7  GCGTC                GACGC(5/10)
HincII        727  GTCAAC               GT[CT]^[GA]AC
HindII        727  GTCAAC               GT[CT]^[GA]AC
HindIII       745  AAGCTT               A^AGCTT
HinfI          39  GACTC                G^A[ACGT]TC
KspI          792  CCGCGG               CCGC^GG
MamI         1125  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI           11  CCATGG               C^CATGG
NspI          193  GCATGC               [GA]CATG^[CT]
PaeR7I        557  CTCGAG               C^TCGAG
PinAI         677  ACCGGT               A^CCGGT
PleI           39  GACTC                GAGTC(4/5)
SacII         792  CCGCGG               CCGC^GG
SbfI          492  CCTGCAGG             CCTGCA^GG
SphI          193  GCATGC               GCATG^C
Sse8387I      492  CCTGCAGG             CCTGCA^GG
StuI         1220  AGGCCT               AGG^CCT
XhoI          557  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccIII        308  TCCGGA               T^CCGGA
              434  TCCGGA              
BalI          672  TGGCCA               TGG^CCA
             1536  TGGCCA              
BbvCI         166  CCTCAGC              CCTCAGC(-5/-2)
              285  GCTGAGG             
BglII         876  AGATCT               A^GATCT
             1130  AGATCT              
BseAI         308  TCCGGA               T^CCGGA
              434  TCCGGA              
BseRI         478  GAGGAG               GAGGAG(10/8)
             1105  GAGGAG              
BsgI         1272  CTGCAC               GTGCAG(16/14)
              385  GTGCAG              
BsmAI         578  GAGAC                GTCTC(1/5)
              994  GAGAC               
BspEI         308  TCCGGA               T^CCGGA
              434  TCCGGA              
BstXI         369  CCAGGACAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              663  CCACGTGTCTGG        
DraIII        931  CACACCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1342  CACACGGTG           
DrdI          529  GACGTGCCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              949  GACCTGCCAGTC        
EarI          202  GAAGAG               CTCTTC(1/4)
              322  GAAGAG              
Ksp632I       202  GAAGAG               CTCTTC(1/4)
              322  GAAGAG              
MboII         202  GAAGA                GAAGA(8/7)
              322  GAAGA               
MluNI         672  TGGCCA               TGG^CCA
             1536  TGGCCA              
MroI          308  TCCGGA               T^CCGGA
              434  TCCGGA              
MscI          672  TGGCCA               TGG^CCA
             1536  TGGCCA              
PflMI         930  CCACACCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1179  CCAAGAACTGG         
SacI          223  GAGCTC               GAGCT^C
             1089  GAGCTC              
SfaNI        1227  GATGC                GCATC(5/9)
               71  GCATC               
SmaI           59  CCCGGG               CCC^GGG
             1016  CCCGGG              
SmlI          475  CTTGAG               C^T[CT][GA]AG
              557  CTCGAG              
Tsp509I       621  AATT                 ^AATT
              846  AATT                
Van91I        930  CCACACCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1179  CCAAGAACTGG         
XmaCI          59  CCCGGG               C^CCGGG
             1016  CCCGGG              
XmaI           59  CCCGGG               C^CCGGG
             1016  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BanII       
BbrPI       BbvI        BcnI        BmyI        BpmI        
BsaAI       BsaJI       BsaWI       BsiHKAI     BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     XcmI        
XhoII       
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