Restriction map for pFN21AB0477
 (Linear) (Six-base) MAPSORT of: KIBB0477.seq  Check: 8996  from: 1  to: 1554



KIBB0477.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     988    1554 
   Size:        988     566
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     927    1105    1554 
   Size:        523     404     178     449

  Fragments arranged by size: 

                 523     449     404     178
 
AflIII A'CryG_T

Cuts at:      0     665    1554 
   Size:        665     889
 
AgeI A'CCGG_T

Cuts at:      0     677    1554 
   Size:        677     877
 
AleI CACnn'nnGTG

Cuts at:      0    1048    1554 
   Size:       1048     506
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1175    1207    1554 
   Size:       1175      32     347

  Fragments arranged by size: 

                1175     347      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     285     411     501     980    1075    1101    1554 
   Size:        285     126      90     479      95      26     453

  Fragments arranged by size: 

                 479     453     285     126      95      90      26
 
ApaI G_GGCC'C

Cuts at:      0     357    1554 
   Size:        357    1197
 
ApoI r'AATT_y

Cuts at:      0     845    1554 
   Size:        845     709
 
AvaI C'yCGr_G

Cuts at:      0      59     557     605    1016    1554 
   Size:         59     498      48     411     538

  Fragments arranged by size: 

                 538     498     411      59      48
 
BanI G'GyrC_C

Cuts at:      0     777    1554 
   Size:        777     777
 
BanII G_rGCy'C

Cuts at:      0     227     357     708    1093    1554 
   Size:        227     130     351     385     461

  Fragments arranged by size: 

                 461     385     351     227     130
 
BbvCI CC'TCA_GC

Cuts at:      0     167     286    1554 
   Size:        167     119    1268

  Fragments arranged by size: 

                1268     167     119
 
BglI GCCn_nnn'nGGC

Cuts at:      0     361    1554 
   Size:        361    1193
 
BglII A'GATC_T

Cuts at:      0     876    1130    1554 
   Size:        876     254     424

  Fragments arranged by size: 

                 876     424     254
 
Bme1580I G_kGCm'C

Cuts at:      0     357     420     536    1433    1554 
   Size:        357      63     116     897     121

  Fragments arranged by size: 

                 897     357     121     116      63
 
BmgBI CAC'GTC

Cuts at:      0     531    1554 
   Size:        531    1023
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1195    1554 
   Size:       1195     359
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     261     572    1107    1554 
   Size:        261     311     535     447

  Fragments arranged by size: 

                 535     447     311     261
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     337     722     957    1102    1123    1414    1426 
   Size:        241      96     385     235     145      21     291      12

Cuts at:   1426    1477    1554 
   Size:         51      77

  Fragments arranged by size: 

                 385     291     241     235     145      96      77      51
                  21      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     167     286    1554 
   Size:        167     119    1268

  Fragments arranged by size: 

                1268     167     119
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496    1554 
   Size:        496    1058
 
BsaAI yAC'GTr

Cuts at:      0     421     666    1485    1554 
   Size:        421     245     819      69

  Fragments arranged by size: 

                 819     421     245      69
 
BsaBI GATnn'nnATC

Cuts at:      0    1129    1554 
   Size:       1129     425
 
BsaWI w'CCGG_w

Cuts at:      0     119     308     434     677    1554 
   Size:        119     189     126     243     877

  Fragments arranged by size: 

                 877     243     189     126     119
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1099    1129    1175    1205    1318    1348    1554 
   Size:       1099      30      46      30     113      30     206

  Fragments arranged by size: 

                1099     206     113      46      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     493    1120    1554 
   Size:        493     627     434

  Fragments arranged by size: 

                 627     493     434
 
BseYI C'CCAG_C

Cuts at:      0     470    1143    1321    1554 
   Size:        470     673     178     233

  Fragments arranged by size: 

                 673     470     233     178
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     406    1257    1554 
   Size:        406     851     297

  Fragments arranged by size: 

                 851     406     297
 
BsiHKAI G_wGCw'C

Cuts at:      0     227     566    1093    1427    1554 
   Size:        227     339     527     334     127

  Fragments arranged by size: 

                 527     339     334     227     127
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     357     420     536     566     708    1093    1427 
   Size:        227     130      63     116      30     142     385     334

Cuts at:   1427    1433    1554 
   Size:          6     121

  Fragments arranged by size: 

                 385     334     227     142     130     121     116      63
                  30       6
 
BspEI T'CCGG_A

Cuts at:      0     308     434    1554 
   Size:        308     126    1120

  Fragments arranged by size: 

                1120     308     126
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     263     500     698     959     988    1554 
   Size:        263     237     198     261      29     566

  Fragments arranged by size: 

                 566     263     261     237     198      29
 
BsrBI CCG'CTC

Cuts at:      0     183    1554 
   Size:        183    1371
 
BsrFI r'CCGG_y

Cuts at:      0     677    1554 
   Size:        677     877
 
BssHII G'CGCG_C

Cuts at:      0     142    1554 
   Size:        142    1412
 
BssSI C'ACGA_G

Cuts at:      0    1426    1554 
   Size:       1426     128
 
BstEII G'GTnAC_C

Cuts at:      0    1254    1554 
   Size:       1254     300
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     376     670    1554 
   Size:        376     294     884

  Fragments arranged by size: 

                 884     376     294
 
BstYI r'GATC_y

Cuts at:      0     305     431     876    1130    1554 
   Size:        305     126     445     254     424

  Fragments arranged by size: 

                 445     424     305     254     126
 
BtgI C'CryG_G

Cuts at:      0      11     792     935    1554 
   Size:         11     781     143     619

  Fragments arranged by size: 

                 781     619     143      11
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1368    1554 
   Size:       1368     186
 
BtsI GCAGTG_nn'

Cuts at:      0     353     806    1554 
   Size:        353     453     748

  Fragments arranged by size: 

                 748     453     353
 
DraIII CAC_nnn'GTG

Cuts at:      0     936    1347    1554 
   Size:        936     411     207

  Fragments arranged by size: 

                 936     411     207
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     535     955    1554 
   Size:        535     420     599

  Fragments arranged by size: 

                 599     535     420
 
EaeI y'GGCC_r

Cuts at:      0     423     672    1536    1554 
   Size:        423     249     864      18

  Fragments arranged by size: 

                 864     423     249      18
 
EarI CTCTTCn'nnn_

Cuts at:      0     197     317    1554 
   Size:        197     120    1237

  Fragments arranged by size: 

                1237     197     120
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     265     742    1554 
   Size:        265     477     812

  Fragments arranged by size: 

                 812     477     265
 
EcoICRI GAG'CTC

Cuts at:      0     225    1091    1554 
   Size:        225     866     463

  Fragments arranged by size: 

                 866     463     225
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     337     523     722     927     957    1102    1105 
   Size:        241      96     186     199     205      30     145       3

Cuts at:   1105    1123    1414    1426    1477    1554 
   Size:         18     291      12      51      77

  Fragments arranged by size: 

                 291     241     205     199     186     145      96      77
                  51      30      18      12       3
 
EcoO109I rG'GnC_Cy

Cuts at:      0     354     393     948    1072    1161    1554 
   Size:        354      39     555     124      89     393

  Fragments arranged by size: 

                 555     393     354     124      89      39
 
FspI TGC'GCA

Cuts at:      0     192    1554 
   Size:        192    1362
 
HaeII r_GCGC'y

Cuts at:      0      24     502     657    1554 
   Size:         24     478     155     897

  Fragments arranged by size: 

                 897     478     155      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      56      88    1099    1131    1554 
   Size:         56      32    1011      32     423

  Fragments arranged by size: 

                1011     423      56      32      32
 
HincII GTy'rAC

Cuts at:      0     729    1554 
   Size:        729     825
 
HindIII A'AGCT_T

Cuts at:      0     745    1554 
   Size:        745     809
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     555     624    1292    1554 
   Size:        101     454      69     668     262

  Fragments arranged by size: 

                 668     454     262     101      69
 
MscI TGG'CCA

Cuts at:      0     674    1538    1554 
   Size:        674     864      16

  Fragments arranged by size: 

                 864     674      16
 
MslI CAynn'nnrTG

Cuts at:      0     769     775    1048    1554 
   Size:        769       6     273     506

  Fragments arranged by size: 

                 769     506     273       6
 
MspA1I CmG'CkG

Cuts at:      0     171     294     334     794     898    1098    1554 
   Size:        171     123      40     460     104     200     456

  Fragments arranged by size: 

                 460     456     200     171     123     104      40
 
NcoI C'CATG_G

Cuts at:      0      11    1554 
   Size:         11    1543
 
NspI r_CATG'y

Cuts at:      0     197    1554 
   Size:        197    1357
 
PasI CC'CwG_GG

Cuts at:      0     461    1075    1554 
   Size:        461     614     479

  Fragments arranged by size: 

                 614     479     461
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     936    1185    1554 
   Size:        936     249     369

  Fragments arranged by size: 

                 936     369     249
 
PfoI T'CCnGG_A

Cuts at:      0    1163    1391    1554 
   Size:       1163     228     163

  Fragments arranged by size: 

                1163     228     163
 
PmlI CAC'GTG

Cuts at:      0     421     666    1485    1554 
   Size:        421     245     819      69

  Fragments arranged by size: 

                 819     421     245      69
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     469     501    1175    1207    1554 
   Size:        469      32     674      32     347

  Fragments arranged by size: 

                 674     469     347      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     393     948    1161    1554 
   Size:        393     555     213     393

  Fragments arranged by size: 

                 555     393     393     213
 
PspOMI G'GGCC_C

Cuts at:      0     353    1554 
   Size:        353    1201
 
PspXI vC'TCGA_Gb

Cuts at:      0     557    1554 
   Size:        557     997
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     115     147    1554 
   Size:        115      32    1407

  Fragments arranged by size: 

                1407     115      32
 
PstI C_TGCA'G

Cuts at:      0     299     497     716    1334    1554 
   Size:        299     198     219     618     220

  Fragments arranged by size: 

                 618     299     220     219     198
 
PvuII CAG'CTG

Cuts at:      0     171     294     334    1098    1554 
   Size:        171     123      40     764     456

  Fragments arranged by size: 

                 764     456     171     123      40
 
SacI G_AGCT'C

Cuts at:      0     227    1093    1554 
   Size:        227     866     461

  Fragments arranged by size: 

                 866     461     227
 
SacII CC_GC'GG

Cuts at:      0     795    1554 
   Size:        795     759
 
SbfI CC_TGCA'GG

Cuts at:      0     497    1554 
   Size:        497    1057
 
SfcI C'TryA_G

Cuts at:      0     104     153     295     493     712    1330    1554 
   Size:        104      49     142     198     219     618     224

  Fragments arranged by size: 

                 618     224     219     198     142     104      49
 
SmaI CCC'GGG

Cuts at:      0      61    1018    1554 
   Size:         61     957     536

  Fragments arranged by size: 

                 957     536      61
 
SmlI C'TyrA_G

Cuts at:      0     475     557    1554 
   Size:        475      82     997

  Fragments arranged by size: 

                 997     475      82
 
SphI G_CATG'C

Cuts at:      0     197    1554 
   Size:        197    1357
 
StuI AGG'CCT

Cuts at:      0    1222    1554 
   Size:       1222     332
 
StyI C'CwwG_G

Cuts at:      0      11      88    1046    1554 
   Size:         11      77     958     508

  Fragments arranged by size: 

                 958     508      77      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     539    1192    1330    1554 
   Size:        539     653     138     224

  Fragments arranged by size: 

                 653     539     224     138
 
TatI w'GTAC_w

Cuts at:      0    1032    1496    1554 
   Size:       1032     464      58

  Fragments arranged by size: 

                1032     464      58
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     619    1182    1318    1554 
   Size:        619     563     136     236

  Fragments arranged by size: 

                 619     563     236     136
 
XhoI C'TCGA_G

Cuts at:      0     557    1554 
   Size:        557     997
 
XmaI C'CCGG_G

Cuts at:      0      59    1016    1554 
   Size:         59     957     538

  Fragments arranged by size: 

                 957     538      59
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AgeI     AleI     AloI    AlwNI     ApaI
     ApoI     AvaI     BanI    BanII    BbvCI     BglI    BglII Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI
    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I     FspI    HaeII    Hin4I   HincII  HindIII
     MmeI     MscI     MslI   MspA1I     NcoI     NspI     PasI    PflMI
     PfoI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PsrI     PstI
    PvuII     SacI    SacII     SbfI     SfcI     SmaI     SmlI     SphI
     StuI     StyI    TaqII     TatI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AhdI     AlfI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BlpI     BmtI     BsaI
    BsaHI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BsrDI    BsrGI
   BstAPI    BstBI  BstZ17I   Bsu36I     ClaI    CspCI     DraI     EagI
    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI    PshAI     PsiI
     PvuI    RsrII     SalI    SanDI     SapI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI
     TstI  Tth111I     XbaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com