(Circular) (Six-base) MAPSORT of: pFN21AB0476.seq Check: 2711 from: 1 to: 7762 pFN21AB0476 7762 bp With 182 enzymes: SgfI * April 15, 2010 09:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5572 278 Size: 53 83 186 936 4036 2468 Fragments arranged by size: 4036 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4681 5105 1937 Size: 2744 424 4594 Fragments arranged by size: 4594 2744 424 Acc65I G'GTAC_C Cuts at: 2010 5083 2010 Size: 3073 4689 AclI AA'CG_TT Cuts at: 1563 5889 6262 1563 Size: 4326 373 3063 Fragments arranged by size: 4326 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2199 2632 2853 2954 4098 4489 4992 5825 Size: 567 433 221 101 1144 391 503 833 Cuts at: 5825 7209 7477 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1144 833 567 503 433 391 268 221 101 AflII C'TTAA_G Cuts at: 820 1017 2320 3053 4139 820 Size: 197 1303 733 1086 4443 Fragments arranged by size: 4443 1303 1086 733 197 AflIII A'CryG_T Cuts at: 1203 3160 3183 6661 7638 1203 Size: 1957 23 3478 977 1327 Fragments arranged by size: 3478 1957 1327 977 23 AgeI A'CCGG_T Cuts at: 6593 6593 Size: 7762 AhdI GACnn_n'nnGTC Cuts at: 6491 6491 Size: 7762 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7762 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7762 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2865 2897 2865 Size: 32 7730 AlwNI CAG_nnn'CTG Cuts at: 4162 5505 7077 4162 Size: 1343 1572 4847 Fragments arranged by size: 4847 1572 1343 ApaLI G'TGCA_C Cuts at: 2279 5819 6975 2279 Size: 3540 1156 3066 Fragments arranged by size: 3540 3066 1156 ApoI r'AATT_y Cuts at: 1493 2654 2732 2864 3377 3566 3590 3604 3734 Size: 1161 78 132 513 189 24 14 130 Cuts at: 3734 4817 4939 4994 5071 5297 5449 7558 1493 Size: 1083 122 55 77 226 152 2109 1697 Fragments arranged by size: 2109 1697 1161 1083 513 226 189 152 132 130 122 78 77 55 24 14 AseI AT'TA_AT Cuts at: 160 1032 3129 6316 160 Size: 872 2097 3187 1606 Fragments arranged by size: 3187 2097 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7762 AvaI C'yCGr_G Cuts at: 1870 1943 3105 5087 1870 Size: 73 1162 1982 4545 Fragments arranged by size: 4545 1982 1162 73 BamHI G'GATC_C Cuts at: 2299 5092 5459 2299 Size: 2793 367 4602 Fragments arranged by size: 4602 2793 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2010 2069 2483 5083 6538 Size: 325 217 627 223 59 414 2600 1455 Cuts at: 6538 618 Size: 1842 Fragments arranged by size: 2600 1842 1455 627 414 325 223 217 59 BanII G_rGCy'C Cuts at: 1391 3106 3801 4434 5081 1391 Size: 1715 695 633 647 4072 Fragments arranged by size: 4072 1715 695 647 633 BbeI G_GCGC'C Cuts at: 2073 2073 Size: 7762 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2187 4248 4495 928 Size: 971 288 2061 247 4195 Fragments arranged by size: 4195 2061 971 288 247 BbvCI CC'TCA_GC Cuts at: 4155 4155 Size: 7762 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5953 5987 1903 Size: 34 50 34 3932 34 3678 Fragments arranged by size: 3932 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3655 4876 5654 6864 3655 Size: 1221 778 1210 4553 Fragments arranged by size: 4553 1221 1210 778 BclI T'GATC_A Cuts at: 1548 1548 Size: 7762 BfrBI ATG'CAT Cuts at: 3827 3827 Size: 7762 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6373 136 Size: 107 122 71 631 605 4701 1525 Fragments arranged by size: 4701 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7757 7757 Size: 7762 BlpI GC'TnA_GC Cuts at: 5179 5179 Size: 7762 Bme1580I G_kGCm'C Cuts at: 1790 2283 2488 2519 5823 6979 1790 Size: 493 205 31 3304 1156 2573 Fragments arranged by size: 3304 2573 1156 493 205 31 BmgBI CAC'GTC Cuts at: 1318 4517 1318 Size: 3199 4563 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3493 4735 5493 Size: 416 53 83 399 24 2076 1242 758 Cuts at: 5493 6451 442 Size: 958 1753 Fragments arranged by size: 2076 1753 1242 958 758 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2021 3845 1056 Size: 965 1824 4973 Fragments arranged by size: 4973 1824 965 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2620 2702 4448 6422 1239 Size: 1381 82 1746 1974 2579 Fragments arranged by size: 2579 1974 1746 1381 82 Bpu10I CC'TnA_GC Cuts at: 4155 4155 Size: 7762 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3207 3815 3871 4561 5887 6752 7050 3207 Size: 608 56 690 1326 865 298 3919 Fragments arranged by size: 3919 1326 865 690 608 298 56 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6425 882 Size: 617 4926 2219 Fragments arranged by size: 4926 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3209 5553 493 Size: 711 2005 2344 2702 Fragments arranged by size: 2702 2344 2005 711 BsaBI GATnn'nnATC Cuts at: 7756 7756 Size: 7762 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 5569 5951 6586 Size: 53 83 186 936 537 3499 382 635 Cuts at: 6586 275 Size: 1451 Fragments arranged by size: 3499 1451 936 635 537 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2086 3520 6195 6593 6867 7014 7425 2086 Size: 1434 2675 398 274 147 411 2423 Fragments arranged by size: 2675 2423 1434 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2081 2111 2158 2188 4143 Size: 30 165 30 678 30 47 30 1955 Cuts at: 4143 4173 1178 Size: 30 4767 Fragments arranged by size: 4767 1955 678 165 47 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3791 1186 Size: 2605 5157 BseYI C'CCAG_C Cuts at: 2045 3066 4743 6965 7461 2045 Size: 1021 1677 2222 496 2346 Fragments arranged by size: 2346 2222 1677 1021 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2015 3231 4157 1163 Size: 573 279 1216 926 4768 Fragments arranged by size: 4768 1216 926 573 279 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5212 5973 7001 1540 Size: 275 186 3211 761 1028 2301 Fragments arranged by size: 3211 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2283 3354 3801 4434 5823 5908 6979 2283 Size: 1071 447 633 1389 85 1071 3066 Fragments arranged by size: 3066 1389 1071 1071 633 447 85 BsmI GAATG_Cn' Cuts at: 2560 3262 5273 5366 2560 Size: 702 2011 93 4956 Fragments arranged by size: 4956 2011 702 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 2488 2519 3106 3354 3801 4434 Size: 399 493 205 31 587 248 447 633 Cuts at: 4434 5081 5823 5908 6979 1391 Size: 647 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 647 633 587 493 447 399 248 205 85 31 BspEI T'CCGG_A Cuts at: 2086 7425 2086 Size: 5339 2423 BspHI T'CATG_A Cuts at: 2467 5651 7381 2467 Size: 3184 1730 2848 Fragments arranged by size: 3184 2848 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5117 844 Size: 4273 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2058 5649 1394 Size: 429 235 3591 3507 Fragments arranged by size: 3591 3507 429 235 BsrDI GCAATG_nn' Cuts at: 65 1248 2855 6257 6431 65 Size: 1183 1607 3402 174 1396 Fragments arranged by size: 3402 1607 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2013 6406 6593 1700 Size: 110 203 4393 187 2869 Fragments arranged by size: 4393 2869 203 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7762 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7762 BssSI C'ACGA_G Cuts at: 5822 6834 5822 Size: 1012 6750 BstAPI GCAn_nnn'nTGC Cuts at: 794 4844 794 Size: 4050 3712 BstBI TT'CG_AA Cuts at: 6566 6566 Size: 7762 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7762 BstXI CCAn_nnnn'nTGG Cuts at: 2764 4891 2764 Size: 2127 5635 BstYI r'GATC_y Cuts at: 1972 2299 5092 5459 5844 5861 7313 7399 7411 Size: 327 2793 367 385 17 1452 86 12 Cuts at: 7411 7757 1972 Size: 346 1977 Fragments arranged by size: 2793 1977 1452 385 367 346 327 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2250 3293 4192 4611 513 Size: 552 1185 1043 899 419 3664 Fragments arranged by size: 3664 1185 1043 899 552 419 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7023 Fragments arranged by size: 7023 683 56 BtsI GCAGTG_nn' Cuts at: 2039 5278 6072 6092 2039 Size: 3239 794 20 3709 Fragments arranged by size: 3709 3239 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7762 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4351 4386 529 Size: 35 3787 35 3905 Fragments arranged by size: 3905 3787 35 35 DraI TTT'AAA Cuts at: 3277 3571 4788 5066 3277 Size: 294 1217 278 5973 Fragments arranged by size: 5973 1217 294 278 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6769 809 Size: 507 5453 1802 Fragments arranged by size: 5453 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 5209 6098 Size: 54 1425 50 275 18 381 2998 889 Cuts at: 6098 7727 8 Size: 1629 43 Fragments arranged by size: 2998 1629 1425 889 381 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5209 1537 Size: 275 3397 4090 Fragments arranged by size: 4090 3397 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3339 3477 4439 4635 5692 1353 Size: 1986 138 962 196 1057 3423 Fragments arranged by size: 3423 1986 1057 962 196 138 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6349 6723 6869 387 Size: 1064 138 4760 374 146 1280 Fragments arranged by size: 4760 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3799 4432 3799 Size: 633 7129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2199 2620 2632 2702 2853 2954 4098 Size: 393 567 421 12 70 151 101 1144 Cuts at: 4098 4448 4489 4992 5825 6422 7209 7477 1239 Size: 350 41 503 833 597 787 268 1524 Fragments arranged by size: 1524 1144 833 787 597 567 503 421 393 350 268 151 101 70 41 12 EcoNI CCTnn'n_nnAGG Cuts at: 3344 4323 3344 Size: 979 6783 EcoO109I rG'GnC_Cy Cuts at: 3029 3218 3029 Size: 189 7573 EcoRI G'AATT_C Cuts at: 4994 5071 5449 4994 Size: 77 378 7307 Fragments arranged by size: 7307 378 77 EcoRV GAT'ATC Cuts at: 4810 4810 Size: 7762 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2249 2912 2249 Size: 663 7099 FspI TGC'GCA Cuts at: 4475 6268 4475 Size: 1793 5969 HaeII r_GCGC'y Cuts at: 1678 2073 2095 6909 1678 Size: 395 22 4814 2531 Fragments arranged by size: 4814 2531 395 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2246 2278 3844 3876 4536 Size: 32 135 32 674 32 1566 32 660 Cuts at: 4536 4568 4722 4754 6403 6435 6477 6509 1373 Size: 32 154 32 1649 32 42 32 2626 Fragments arranged by size: 2626 1649 1566 674 660 154 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4682 5106 5352 669 Size: 988 281 2744 424 246 3079 Fragments arranged by size: 3079 2744 988 424 281 246 HindIII A'AGCT_T Cuts at: 748 4596 748 Size: 3848 3914 HpaI GTT'AAC Cuts at: 5352 5352 Size: 7762 KasI G'GCGC_C Cuts at: 2069 2069 Size: 7762 KpnI G_GTAC'C Cuts at: 2014 5087 2014 Size: 3073 4689 MfeI C'AATT_G Cuts at: 2612 5361 2612 Size: 2749 5013 MluI A'CGCG_T Cuts at: 7638 7638 Size: 7762 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2277 2781 4891 6876 7060 1305 Size: 972 504 2110 1985 184 2007 Fragments arranged by size: 2110 2007 1985 972 504 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2213 10 Size: 54 1425 343 381 5559 Fragments arranged by size: 5559 1425 381 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2331 3131 4044 5720 6079 6238 518 Size: 648 1165 800 913 1676 359 159 2042 Fragments arranged by size: 2042 1676 1165 913 800 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3359 4291 4509 5178 5855 7003 7248 1606 Size: 1753 932 218 669 677 1148 245 2120 Fragments arranged by size: 2120 1753 1148 932 677 669 245 218 NarI GG'CG_CC Cuts at: 2070 2070 Size: 7762 NcoI C'CATG_G Cuts at: 513 1065 4192 4611 513 Size: 552 3127 419 3664 Fragments arranged by size: 3664 3127 552 419 NdeI CA'TA_TG Cuts at: 387 2340 3829 387 Size: 1953 1489 4320 Fragments arranged by size: 4320 1953 1489 NheI G'CTAG_C Cuts at: 1052 2017 3841 1052 Size: 965 1824 4973 Fragments arranged by size: 4973 1824 965 NotI GC'GGCC_GC Cuts at: 5209 5209 Size: 7762 NsiI A_TGCA'T Cuts at: 3829 3829 Size: 7762 NspI r_CATG'y Cuts at: 1132 1229 2408 3164 3187 4049 4262 4402 5120 Size: 97 1179 756 23 862 213 140 718 Cuts at: 5120 6665 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1179 862 756 718 213 140 97 23 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7762 PciI A'CATG_T Cuts at: 3160 3183 6661 3160 Size: 23 3478 4261 Fragments arranged by size: 4261 3478 23 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7762 PfoI T'CCnGG_A Cuts at: 3031 3031 Size: 7762 PmeI GTTT'AAAC Cuts at: 5066 5066 Size: 7762 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5831 5863 7344 7376 5831 Size: 32 1481 32 6217 Fragments arranged by size: 6217 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3029 3218 3029 Size: 189 7573 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7762 PsiI TTA'TAA Cuts at: 5332 5332 Size: 7762 PstI C_TGCA'G Cuts at: 830 5114 830 Size: 4284 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7762 PvuII CAG'CTG Cuts at: 4291 4291 Size: 7762 SacI G_AGCT'C Cuts at: 3801 4434 3801 Size: 633 7129 SalI G'TCGA_C Cuts at: 1936 4680 5104 1936 Size: 2744 424 4594 Fragments arranged by size: 4594 2744 424 SapI GCTCTTCn'nnn_ Cuts at: 4439 4439 Size: 7762 SbfI CC_TGCA'GG Cuts at: 5114 5114 Size: 7762 ScaI AGT'ACT Cuts at: 2229 6010 7722 2229 Size: 3781 1712 2269 Fragments arranged by size: 3781 2269 1712 SfcI C'TryA_G Cuts at: 826 1045 2625 5110 6245 6926 7117 826 Size: 219 1580 2485 1135 681 191 1471 Fragments arranged by size: 2485 1580 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2071 2071 Size: 7762 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7762 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7762 SmaI CCC'GGG Cuts at: 1872 5089 1872 Size: 3217 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2320 3053 3222 3794 3850 4139 Size: 197 926 377 733 169 572 56 289 Cuts at: 4139 4540 5866 6767 7029 820 Size: 401 1326 901 262 1553 Fragments arranged by size: 1553 1326 926 901 733 572 401 377 289 262 197 169 56 SnaBI TAC'GTA Cuts at: 493 5553 493 Size: 5060 2702 SpeI A'CTAG_T Cuts at: 152 2429 152 Size: 2277 5485 SphI G_CATG'C Cuts at: 1132 2408 4049 5120 1132 Size: 1276 1641 1071 3774 Fragments arranged by size: 3774 1641 1276 1071 SspI AAT'ATT Cuts at: 5 52 2311 3324 3365 5686 5 Size: 47 2259 1013 41 2321 2081 Fragments arranged by size: 2321 2259 2081 1013 47 41 StuI AGG'CCT Cuts at: 1517 4145 4646 1517 Size: 2628 501 4633 Fragments arranged by size: 4633 2628 501 StyI C'CwwG_G Cuts at: 513 1065 4192 4479 4611 4800 4850 5201 513 Size: 552 3127 287 132 189 50 351 3074 Fragments arranged by size: 3127 3074 552 351 287 189 132 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3158 5006 5789 5806 5959 6144 6589 Size: 1255 1578 1848 783 17 153 185 445 Cuts at: 6589 325 Size: 1498 Fragments arranged by size: 1848 1578 1498 1255 783 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2227 3501 4053 Size: 274 80 33 51 1443 250 1274 552 Cuts at: 4053 6008 7720 96 Size: 1955 1712 138 Fragments arranged by size: 1955 1712 1443 1274 552 274 250 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2081 2113 7722 7754 2081 Size: 32 5609 32 2089 Fragments arranged by size: 5609 2089 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7762 XbaI T'CTAG_A Cuts at: 5098 5098 Size: 7762 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4486 4486 Size: 7762 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7762 XmaI C'CCGG_G Cuts at: 1870 5087 1870 Size: 3217 4545 XmnI GAAnn'nnTTC Cuts at: 3594 4185 4977 5891 3594 Size: 591 792 914 5465 Fragments arranged by size: 5465 914 792 591 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5570 276 Size: 53 83 186 936 4036 2468 Fragments arranged by size: 4036 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI