(Circular) (Six-base) MAPSORT of: pFN21AB0469.seq Check: 3393 from: 1 to: 6250 pFN21AB0469 6250 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4060 278 Size: 53 83 186 936 2524 2468 Fragments arranged by size: 2524 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3593 1937 Size: 1656 4594 Acc65I G'GTAC_C Cuts at: 2425 3571 2425 Size: 1146 5104 AclI AA'CG_TT Cuts at: 1563 4377 4750 1563 Size: 2814 373 3063 Fragments arranged by size: 3063 2814 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2244 3326 3356 4313 5697 5965 1632 Size: 612 1082 30 957 1384 268 1917 Fragments arranged by size: 1917 1384 1082 957 612 268 30 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6053 AflIII A'CryG_T Cuts at: 1203 5149 6126 1203 Size: 3946 977 1327 Fragments arranged by size: 3946 1327 977 AgeI A'CCGG_T Cuts at: 5081 5081 Size: 6250 AhdI GACnn_n'nnGTC Cuts at: 4979 4979 Size: 6250 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6250 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6250 AlwNI CAG_nnn'CTG Cuts at: 3993 5565 3993 Size: 1572 4678 ApaI G_GGCC'C Cuts at: 2093 2129 2093 Size: 36 6214 ApaLI G'TGCA_C Cuts at: 4307 5463 4307 Size: 1156 5094 ApoI r'AATT_y Cuts at: 1493 2397 2553 3217 3559 3785 3937 6046 1493 Size: 904 156 664 342 226 152 2109 1697 Fragments arranged by size: 2109 1697 904 664 342 226 156 152 AseI AT'TA_AT Cuts at: 160 1032 3123 4804 160 Size: 872 2091 1681 1606 Fragments arranged by size: 2091 1681 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 AvaI C'yCGr_G Cuts at: 1870 1943 2013 2092 3575 1870 Size: 73 70 79 1483 4545 Fragments arranged by size: 4545 1483 79 73 70 BamHI G'GATC_C Cuts at: 3580 3947 3580 Size: 367 5883 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 2425 3571 5026 618 Size: 325 217 627 261 377 1146 1455 1842 Fragments arranged by size: 1842 1455 1146 627 377 325 261 217 BanII G_rGCy'C Cuts at: 1391 2093 2129 3466 3569 1391 Size: 702 36 1337 103 4072 Fragments arranged by size: 4072 1337 702 103 36 BbeI G_GCGC'C Cuts at: 2052 2052 Size: 6250 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3212 3314 928 Size: 971 1313 102 3864 Fragments arranged by size: 3864 1313 971 102 BbvCI CC'TCA_GC Cuts at: 2319 3078 2319 Size: 759 5491 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4441 4475 1903 Size: 34 2504 34 3678 Fragments arranged by size: 3678 2504 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4142 5352 4142 Size: 1210 5040 BclI T'GATC_A Cuts at: 1548 2946 3151 1548 Size: 1398 205 4647 Fragments arranged by size: 4647 1398 205 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4861 136 Size: 107 122 71 631 605 337 2852 1525 Fragments arranged by size: 2852 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3039 6245 3039 Size: 3206 3044 BlpI GC'TnA_GC Cuts at: 3329 3667 3329 Size: 338 5912 Bme1580I G_kGCm'C Cuts at: 1790 2093 2129 4311 5467 1790 Size: 303 36 2182 1156 2573 Fragments arranged by size: 2573 2182 1156 303 36 BmgBI CAC'GTC Cuts at: 1318 2836 1318 Size: 1518 4732 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2275 2878 3373 Size: 416 53 83 399 24 858 603 495 Cuts at: 3373 3981 4939 442 Size: 608 958 1753 Fragments arranged by size: 1753 958 858 608 603 495 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6250 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2672 2972 4910 1239 Size: 1433 300 1938 2579 Fragments arranged by size: 2579 1938 1433 300 Bpu10I CC'TnA_GC Cuts at: 2319 3078 3465 2319 Size: 759 387 5104 Fragments arranged by size: 5104 759 387 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3377 4375 5240 5538 3377 Size: 998 865 298 4089 Fragments arranged by size: 4089 998 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2176 2302 2795 3336 4913 882 Size: 617 677 126 493 541 1577 2219 Fragments arranged by size: 2219 1577 677 617 541 493 126 BsaAI yAC'GTr Cuts at: 493 1204 4041 493 Size: 711 2837 2702 Fragments arranged by size: 2837 2702 711 BsaBI GATnn'nnATC Cuts at: 6244 6244 Size: 6250 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 4057 4439 5074 Size: 53 83 186 936 516 2008 382 635 Cuts at: 5074 275 Size: 1451 Fragments arranged by size: 2008 1451 936 635 516 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3540 4683 5081 5355 5502 5913 3540 Size: 1143 398 274 147 411 3877 Fragments arranged by size: 3877 1143 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2970 3000 3461 3476 3491 Size: 30 165 30 1567 30 461 15 15 Cuts at: 3491 3506 1178 Size: 15 3922 Fragments arranged by size: 3922 1567 461 165 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3440 3443 1186 Size: 2254 3 3993 Fragments arranged by size: 3993 2254 3 BseYI C'CCAG_C Cuts at: 5453 5949 5453 Size: 496 5754 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3403 3521 1163 Size: 573 1667 118 3892 Fragments arranged by size: 3892 1667 573 118 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2828 3700 4461 5489 1540 Size: 275 186 827 872 761 1028 2301 Fragments arranged by size: 2301 1028 872 827 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4311 4396 5467 4311 Size: 85 1071 5094 Fragments arranged by size: 5094 1071 85 BsmI GAATG_Cn' Cuts at: 3761 3854 3761 Size: 93 6157 BsmBI CGTCTCn'nnnn_ Cuts at: 2826 3502 2826 Size: 676 5574 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2129 3466 3569 4311 4396 5467 Size: 399 303 36 1337 103 742 85 1071 Cuts at: 5467 1391 Size: 2174 Fragments arranged by size: 2174 1337 1071 742 399 303 103 85 36 BspEI T'CCGG_A Cuts at: 3540 5913 3540 Size: 2373 3877 BspHI T'CATG_A Cuts at: 2487 4139 5869 2487 Size: 1652 1730 2868 Fragments arranged by size: 2868 1730 1652 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2320 2323 3039 3605 844 Size: 1476 3 716 566 3489 Fragments arranged by size: 3489 1476 716 566 3 BsrBI CCG'CTC Cuts at: 1394 1823 2154 2171 3004 4137 1394 Size: 429 331 17 833 1133 3507 Fragments arranged by size: 3507 1133 833 429 331 17 BsrDI GCAATG_nn' Cuts at: 65 1248 4745 4919 65 Size: 1183 3497 174 1396 Fragments arranged by size: 3497 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4894 5081 1700 Size: 110 3084 187 2869 Fragments arranged by size: 3084 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6250 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6250 BssSI C'ACGA_G Cuts at: 2999 4310 5322 2999 Size: 1311 1012 3927 Fragments arranged by size: 3927 1311 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6250 BstBI TT'CG_AA Cuts at: 5054 5054 Size: 6250 BstEII G'GTnAC_C Cuts at: 1184 3416 1184 Size: 2232 4018 BstYI r'GATC_y Cuts at: 1972 2325 2449 3039 3072 3273 3580 3947 4332 Size: 353 124 590 33 201 307 367 385 Cuts at: 4332 4349 5801 5887 5899 6245 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 590 385 367 353 346 307 201 124 86 33 17 12 Bsu36I CC'TnA_GG Cuts at: 2059 2217 3348 2059 Size: 158 1131 4961 Fragments arranged by size: 4961 1131 158 BtgI C'CryG_G Cuts at: 513 1065 2004 2229 513 Size: 552 939 225 4534 Fragments arranged by size: 4534 939 552 225 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5511 Fragments arranged by size: 5511 683 56 BtsI GCAGTG_nn' Cuts at: 3234 3766 4560 4580 3234 Size: 532 794 20 4904 Fragments arranged by size: 4904 794 532 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6250 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2942 2977 529 Size: 35 2378 35 3802 Fragments arranged by size: 3802 2378 35 35 DraI TTT'AAA Cuts at: 2890 3554 2890 Size: 664 5586 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5257 809 Size: 507 3941 1802 Fragments arranged by size: 3941 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2825 3697 4586 Size: 54 1425 50 275 18 995 872 889 Cuts at: 4586 6215 8 Size: 1629 43 Fragments arranged by size: 1629 1425 995 889 872 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2825 3697 1537 Size: 275 1013 872 4090 Fragments arranged by size: 4090 1013 872 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2710 4180 1353 Size: 1357 1470 3423 Fragments arranged by size: 3423 1470 1357 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2112 4837 5211 5357 387 Size: 1064 138 523 2725 374 146 1280 Fragments arranged by size: 2725 1280 1064 523 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2244 2672 2972 3326 3356 4313 4910 Size: 393 612 428 300 354 30 957 597 Cuts at: 4910 5697 5965 1239 Size: 787 268 1524 Fragments arranged by size: 1524 957 787 612 597 428 393 354 300 268 30 EcoO109I rG'GnC_Cy Cuts at: 2108 2222 2234 2992 3388 2108 Size: 114 12 758 396 4970 Fragments arranged by size: 4970 758 396 114 12 EcoRI G'AATT_C Cuts at: 3217 3559 3937 3217 Size: 342 378 5530 Fragments arranged by size: 5530 378 342 EcoRV GAT'ATC Cuts at: 2506 2506 Size: 6250 FspI TGC'GCA Cuts at: 4756 4756 Size: 6250 HaeII r_GCGC'y Cuts at: 1678 2052 3436 5397 1678 Size: 374 1384 1961 2531 Fragments arranged by size: 2531 1961 1384 374 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3244 3276 4891 4923 4965 Size: 32 135 32 1672 32 1615 32 42 Cuts at: 4965 4997 1373 Size: 32 2626 Fragments arranged by size: 2626 1672 1615 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3142 3594 3840 669 Size: 988 281 1204 452 246 3079 Fragments arranged by size: 3079 1204 988 452 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6250 HpaI GTT'AAC Cuts at: 3840 3840 Size: 6250 KasI G'GCGC_C Cuts at: 2048 2048 Size: 6250 KpnI G_GTAC'C Cuts at: 2429 3575 2429 Size: 1146 5104 MfeI C'AATT_G Cuts at: 3849 3849 Size: 6250 MluI A'CGCG_T Cuts at: 6126 6126 Size: 6250 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2290 5364 5548 1305 Size: 985 3074 184 2007 Fragments arranged by size: 3074 2007 985 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4428 Fragments arranged by size: 4428 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4208 4567 4726 518 Size: 648 3042 359 159 2042 Fragments arranged by size: 3042 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3082 3304 3316 3666 4343 5491 5736 1606 Size: 1476 222 12 350 677 1148 245 2120 Fragments arranged by size: 2120 1476 1148 677 350 245 222 12 NarI GG'CG_CC Cuts at: 2049 2049 Size: 6250 NcoI C'CATG_G Cuts at: 513 1065 2004 2229 513 Size: 552 939 225 4534 Fragments arranged by size: 4534 939 552 225 NdeI CA'TA_TG Cuts at: 387 387 Size: 6250 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6250 NotI GC'GGCC_GC Cuts at: 3697 3697 Size: 6250 NspI r_CATG'y Cuts at: 1132 1229 3608 5153 1132 Size: 97 2379 1545 2229 Fragments arranged by size: 2379 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3459 3532 1348 Size: 2111 73 4066 Fragments arranged by size: 4066 2111 73 PciI A'CATG_T Cuts at: 5149 5149 Size: 6250 PflMI CCAn_nnn'nTGG Cuts at: 1108 2032 1108 Size: 924 5326 PfoI T'CCnGG_A Cuts at: 2649 2988 2649 Size: 339 5911 PmeI GTTT'AAAC Cuts at: 3554 3554 Size: 6250 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4319 4351 5832 5864 4319 Size: 32 1481 32 4705 Fragments arranged by size: 4705 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2222 2234 2992 3388 2222 Size: 12 758 396 5084 Fragments arranged by size: 5084 758 396 12 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6250 PsiI TTA'TAA Cuts at: 3820 3820 Size: 6250 PspOMI G'GGCC_C Cuts at: 2089 2125 2089 Size: 36 6214 PstI C_TGCA'G Cuts at: 830 3036 3602 830 Size: 2206 566 3478 Fragments arranged by size: 3478 2206 566 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6250 PvuII CAG'CTG Cuts at: 3304 3304 Size: 6250 SalI G'TCGA_C Cuts at: 1936 3592 1936 Size: 1656 4594 SanDI GG'GwC_CC Cuts at: 2234 2234 Size: 6250 SbfI CC_TGCA'GG Cuts at: 3602 3602 Size: 6250 ScaI AGT'ACT Cuts at: 4498 6210 4498 Size: 1712 4538 SexAI A'CCwGG_T Cuts at: 2185 2185 Size: 6250 SfcI C'TryA_G Cuts at: 826 1045 3032 3598 4733 5414 5605 826 Size: 219 1987 566 1135 681 191 1471 Fragments arranged by size: 1987 1471 1135 681 566 219 191 SfoI GGC'GCC Cuts at: 2050 2050 Size: 6250 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6250 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6250 SmaI CCC'GGG Cuts at: 1872 2015 2094 3577 1872 Size: 143 79 1483 4545 Fragments arranged by size: 4545 1483 143 79 SmlI C'TyrA_G Cuts at: 820 1017 1943 3392 4354 5255 5517 820 Size: 197 926 1449 962 901 262 1553 Fragments arranged by size: 1553 1449 962 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4041 493 Size: 3548 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6250 SphI G_CATG'C Cuts at: 1132 3608 1132 Size: 2476 3774 SrfI GCCC'GGGC Cuts at: 2015 2094 2015 Size: 79 6171 SspI AAT'ATT Cuts at: 5 52 4174 5 Size: 47 4122 2081 Fragments arranged by size: 4122 2081 47 StuI AGG'CCT Cuts at: 1517 2441 2516 1517 Size: 924 75 5251 Fragments arranged by size: 5251 924 75 StyI C'CwwG_G Cuts at: 513 1065 2004 2085 2229 2721 3495 3689 513 Size: 552 939 81 144 492 774 194 3074 Fragments arranged by size: 3074 939 774 552 492 194 144 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2819 4277 4294 4447 4632 5077 325 Size: 1255 1239 1458 17 153 185 445 1498 Fragments arranged by size: 1498 1458 1255 1239 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2849 4496 6208 Size: 274 80 33 51 1443 872 1647 1712 Cuts at: 6208 96 Size: 138 Fragments arranged by size: 1712 1647 1443 872 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2560 2592 2970 3002 3461 3493 6210 6242 2560 Size: 32 378 32 459 32 2717 32 2568 Fragments arranged by size: 2717 2568 459 378 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6250 XbaI T'CTAG_A Cuts at: 3262 3586 3262 Size: 324 5926 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2804 2804 Size: 6250 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6250 XmaI C'CCGG_G Cuts at: 1870 2013 2092 3575 1870 Size: 143 79 1483 4545 Fragments arranged by size: 4545 1483 143 79 XmnI GAAnn'nnTTC Cuts at: 4379 4379 Size: 6250 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4058 276 Size: 53 83 186 936 2524 2468 Fragments arranged by size: 2524 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SwaI