(Circular) (Six-base) MAPSORT of: pFN21AB0458.seq Check: 5006 from: 1 to: 7552 pFN21AB0458 7552 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2903 2903 Size: 7552 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5362 278 Size: 53 83 186 936 3826 2468 Fragments arranged by size: 3826 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4895 1937 Size: 2958 4594 Acc65I G'GTAC_C Cuts at: 4873 4873 Size: 7552 AclI AA'CG_TT Cuts at: 1563 5679 6052 1563 Size: 4116 373 3063 Fragments arranged by size: 4116 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2814 2874 3929 3968 4658 5615 6999 7267 Size: 1182 60 1055 39 690 957 1384 268 Cuts at: 7267 1632 Size: 1917 Fragments arranged by size: 1917 1384 1182 1055 957 690 268 60 39 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7355 AflIII A'CryG_T Cuts at: 1203 2163 3341 6451 7428 1203 Size: 960 1178 3110 977 1327 Fragments arranged by size: 3110 1327 1178 977 960 AgeI A'CCGG_T Cuts at: 6383 6383 Size: 7552 AhdI GACnn_n'nnGTC Cuts at: 6281 6281 Size: 7552 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7552 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2257 4393 1184 Size: 1073 2136 4343 Fragments arranged by size: 4343 2136 1073 AlwNI CAG_nnn'CTG Cuts at: 2696 5295 6867 2696 Size: 2599 1572 3381 Fragments arranged by size: 3381 2599 1572 ApaLI G'TGCA_C Cuts at: 4586 5609 6765 4586 Size: 1023 1156 5373 Fragments arranged by size: 5373 1156 1023 ApoI r'AATT_y Cuts at: 1493 2884 2902 3032 3206 3721 4499 4670 4861 Size: 1391 18 130 174 515 778 171 191 Cuts at: 4861 5087 5239 7348 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1391 778 515 226 191 174 171 152 130 18 AseI AT'TA_AT Cuts at: 160 1032 6106 160 Size: 872 5074 1606 Fragments arranged by size: 5074 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7552 AvaI C'yCGr_G Cuts at: 1870 1943 2305 3127 3521 4053 4877 1870 Size: 73 362 822 394 532 824 4545 Fragments arranged by size: 4545 824 822 532 394 362 73 AvrII C'CTAG_G Cuts at: 2554 2554 Size: 7552 BamHI G'GATC_C Cuts at: 4251 4882 5249 4251 Size: 631 367 6554 Fragments arranged by size: 6554 631 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2389 3754 3863 4873 6328 Size: 325 217 627 602 1365 109 1010 1455 Cuts at: 6328 618 Size: 1842 Fragments arranged by size: 1842 1455 1365 1010 627 602 325 217 109 BanII G_rGCy'C Cuts at: 1391 3243 3483 4158 4241 4599 4871 1391 Size: 1852 240 675 83 358 272 4072 Fragments arranged by size: 4072 1852 675 358 272 240 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4646 928 Size: 971 2747 3834 Fragments arranged by size: 3834 2747 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5743 5777 1903 Size: 34 3806 34 3678 Fragments arranged by size: 3806 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5444 6654 5444 Size: 1210 6342 BclI T'GATC_A Cuts at: 1548 1548 Size: 7552 BfrBI ATG'CAT Cuts at: 4288 4288 Size: 7552 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6163 136 Size: 107 122 71 631 605 4491 1525 Fragments arranged by size: 4491 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2923 3786 7547 2923 Size: 863 3761 2928 Fragments arranged by size: 3761 2928 863 BlpI GC'TnA_GC Cuts at: 4969 4969 Size: 7552 Bme1580I G_kGCm'C Cuts at: 1790 3759 4590 5613 6769 1790 Size: 1969 831 1023 1156 2573 Fragments arranged by size: 2573 1969 1156 1023 831 BmgBI CAC'GTC Cuts at: 1318 2164 4012 4021 1318 Size: 846 1848 9 4849 Fragments arranged by size: 4849 1848 846 9 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3682 4801 4816 Size: 416 53 83 399 24 2265 1119 15 Cuts at: 4816 5283 6241 442 Size: 467 958 1753 Fragments arranged by size: 2265 1753 1119 958 467 416 399 83 53 24 15 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7552 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2068 2068 Size: 7552 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4583 6212 1239 Size: 3344 1629 2579 Fragments arranged by size: 3344 2579 1629 Bpu10I CC'TnA_GC Cuts at: 3482 3482 Size: 7552 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3257 4475 5677 6542 6840 3257 Size: 1218 1202 865 298 3969 Fragments arranged by size: 3969 1218 1202 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2051 2412 4251 6215 882 Size: 617 552 361 1839 1964 2219 Fragments arranged by size: 2219 1964 1839 617 552 361 BsaAI yAC'GTr Cuts at: 493 1204 3617 5343 493 Size: 711 2413 1726 2702 Fragments arranged by size: 2702 2413 1726 711 BsaBI GATnn'nnATC Cuts at: 7546 7546 Size: 7552 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3394 5359 5741 6376 Size: 53 83 186 936 1861 1965 382 635 Cuts at: 6376 275 Size: 1451 Fragments arranged by size: 1965 1861 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2449 3973 5985 6383 6657 6804 7215 2449 Size: 1524 2012 398 274 147 411 2786 Fragments arranged by size: 2786 2012 1524 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3293 3323 4124 4154 1178 Size: 30 165 30 1890 30 801 30 4576 Fragments arranged by size: 4576 1890 801 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2041 3190 4607 1186 Size: 855 1149 1417 4131 Fragments arranged by size: 4131 1417 1149 855 BseYI C'CCAG_C Cuts at: 2572 3593 3868 6755 7251 2572 Size: 1021 275 2887 496 2873 Fragments arranged by size: 2887 2873 1021 496 275 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2459 3593 1163 Size: 573 723 1134 5122 Fragments arranged by size: 5122 1134 723 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5002 5763 6791 1540 Size: 275 186 3001 761 1028 2301 Fragments arranged by size: 3001 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3243 3991 4241 4590 4599 4748 5613 5698 6769 Size: 748 250 349 9 149 865 85 1071 Cuts at: 6769 3243 Size: 4026 Fragments arranged by size: 4026 1071 865 748 349 250 149 85 9 BsmI GAATG_Cn' Cuts at: 3450 5063 5156 3450 Size: 1613 93 5846 Fragments arranged by size: 5846 1613 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3243 3483 3759 3991 4158 4241 4590 Size: 399 1453 240 276 232 167 83 349 Cuts at: 4590 4599 4748 4871 5613 5698 6769 1391 Size: 9 149 123 742 85 1071 2174 Fragments arranged by size: 2174 1453 1071 742 399 349 276 240 232 167 149 123 85 83 9 BspEI T'CCGG_A Cuts at: 2449 3973 7215 2449 Size: 1524 3242 2786 Fragments arranged by size: 3242 2786 1524 BspHI T'CATG_A Cuts at: 5441 7171 5441 Size: 1730 5822 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2287 2903 4626 4907 844 Size: 1443 616 1723 281 3489 Fragments arranged by size: 3489 1723 1443 616 281 BsrBI CCG'CTC Cuts at: 1394 1823 5439 1394 Size: 429 3616 3507 Fragments arranged by size: 3616 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2782 4628 4714 6047 6221 65 Size: 1183 1534 1846 86 1333 174 1396 Fragments arranged by size: 1846 1534 1396 1333 1183 174 86 BsrFI r'CCGG_y Cuts at: 1700 1810 6196 6383 1700 Size: 110 4386 187 2869 Fragments arranged by size: 4386 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7552 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7552 BssSI C'ACGA_G Cuts at: 2081 3464 4157 4589 5612 6624 2081 Size: 1383 693 432 1023 1012 3009 Fragments arranged by size: 3009 1383 1023 1012 693 432 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7552 BstBI TT'CG_AA Cuts at: 4176 6356 4176 Size: 2180 5372 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7552 BstXI CCAn_nnnn'nTGG Cuts at: 2068 2821 3691 2068 Size: 753 870 5929 Fragments arranged by size: 5929 870 753 BstYI r'GATC_y Cuts at: 1972 2606 2722 2923 3180 3786 4251 4882 5249 Size: 634 116 201 257 606 465 631 367 Cuts at: 5249 5634 5651 7103 7189 7201 7547 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 634 631 606 465 385 367 346 257 201 116 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2371 2371 Size: 7552 BtgI C'CryG_G Cuts at: 513 1065 2004 2661 3706 513 Size: 552 939 657 1045 4359 Fragments arranged by size: 4359 1045 939 657 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2128 3887 4470 4828 487 Size: 683 56 902 1759 583 358 3211 Fragments arranged by size: 3211 1759 902 683 583 358 56 BtsI GCAGTG_nn' Cuts at: 2025 2154 2340 5068 5862 5882 2025 Size: 129 186 2728 794 20 3695 Fragments arranged by size: 3695 2728 794 186 129 20 ClaI AT'CG_AT Cuts at: 1554 3657 1554 Size: 2103 5449 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3033 3068 3278 3313 529 Size: 35 2469 35 210 35 4768 Fragments arranged by size: 4768 2469 210 35 35 35 DraI TTT'AAA Cuts at: 4856 4856 Size: 7552 DraIII CAC_nnn'GTG Cuts at: 2624 3516 3986 2624 Size: 892 470 6190 Fragments arranged by size: 6190 892 470 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2168 2565 6559 809 Size: 507 852 397 3994 1802 Fragments arranged by size: 3994 1802 852 507 397 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 3693 4999 Size: 54 1425 50 275 18 285 1578 1306 Cuts at: 4999 5888 7517 8 Size: 889 1629 43 Fragments arranged by size: 1629 1578 1425 1306 889 285 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4999 1537 Size: 275 3187 4090 Fragments arranged by size: 4090 3187 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2037 3800 5482 1353 Size: 684 1763 1682 3423 Fragments arranged by size: 3423 1763 1682 684 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6139 6513 6659 387 Size: 1064 138 4550 374 146 1280 Fragments arranged by size: 4550 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3241 4239 4597 3241 Size: 998 358 6196 Fragments arranged by size: 6196 998 358 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2814 2874 3929 3968 4583 4658 5615 Size: 393 1182 60 1055 39 615 75 957 Cuts at: 5615 6212 6999 7267 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1182 1055 957 787 615 597 393 268 75 60 39 EcoO109I rG'GnC_Cy Cuts at: 2848 3145 3586 2848 Size: 297 441 6814 Fragments arranged by size: 6814 441 297 EcoRI G'AATT_C Cuts at: 4499 4670 4861 5239 4499 Size: 171 191 378 6812 Fragments arranged by size: 6812 378 191 171 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2778 3511 2778 Size: 733 6819 FspI TGC'GCA Cuts at: 4720 6058 4720 Size: 1338 6214 HaeII r_GCGC'y Cuts at: 1678 6699 1678 Size: 5021 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4273 4305 4393 4425 6193 Size: 32 135 32 2701 32 88 32 1768 Cuts at: 6193 6225 6267 6299 1373 Size: 32 42 32 2626 Fragments arranged by size: 2701 2626 1768 135 88 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4276 4896 5142 669 Size: 988 281 2338 620 246 3079 Fragments arranged by size: 3079 2338 988 620 281 246 HindIII A'AGCT_T Cuts at: 748 3458 748 Size: 2710 4842 HpaI GTT'AAC Cuts at: 5142 5142 Size: 7552 KpnI G_GTAC'C Cuts at: 4877 4877 Size: 7552 MfeI C'AATT_G Cuts at: 3045 5151 3045 Size: 2106 5446 MluI A'CGCG_T Cuts at: 7428 7428 Size: 7552 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2155 3362 3578 6666 6850 1305 Size: 850 1207 216 3088 184 2007 Fragments arranged by size: 3088 2007 1207 850 216 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 3695 10 Size: 54 1425 343 285 1578 3867 Fragments arranged by size: 3867 1578 1425 343 285 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2645 3368 4019 4283 4340 5510 Size: 648 981 498 723 651 264 57 1170 Cuts at: 5510 5869 6028 518 Size: 359 159 2042 Fragments arranged by size: 2042 1170 981 723 651 648 498 359 264 159 57 MspA1I CmG'CkG Cuts at: 1606 2572 2821 3194 4765 4968 5645 6793 7038 Size: 966 249 373 1571 203 677 1148 245 Cuts at: 7038 1606 Size: 2120 Fragments arranged by size: 2120 1571 1148 966 677 373 249 245 203 NcoI C'CATG_G Cuts at: 513 1065 2004 2661 513 Size: 552 939 657 5404 Fragments arranged by size: 5404 939 657 552 NdeI CA'TA_TG Cuts at: 387 3350 387 Size: 2963 4589 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7552 NotI GC'GGCC_GC Cuts at: 4999 4999 Size: 7552 NsiI A_TGCA'T Cuts at: 4290 4290 Size: 7552 NspI r_CATG'y Cuts at: 1132 1229 3124 3345 3367 4910 6455 1132 Size: 97 1895 221 22 1543 1545 2229 Fragments arranged by size: 2229 1895 1545 1543 221 97 22 PasI CC'CwG_GG Cuts at: 1348 2582 1348 Size: 1234 6318 PciI A'CATG_T Cuts at: 3341 6451 3341 Size: 3110 4442 PflMI CCAn_nnn'nTGG Cuts at: 1108 3436 4015 4248 1108 Size: 2328 579 233 4412 Fragments arranged by size: 4412 2328 579 233 PmeI GTTT'AAAC Cuts at: 4856 4856 Size: 7552 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5621 5653 7134 7166 5621 Size: 32 1481 32 6007 Fragments arranged by size: 6007 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2848 3586 2848 Size: 738 6814 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7552 PsiI TTA'TAA Cuts at: 5122 5122 Size: 7552 PstI C_TGCA'G Cuts at: 830 2911 4904 830 Size: 2081 1993 3478 Fragments arranged by size: 3478 2081 1993 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7552 PvuII CAG'CTG Cuts at: 2572 3194 4765 2572 Size: 622 1571 5359 Fragments arranged by size: 5359 1571 622 SacI G_AGCT'C Cuts at: 3243 4241 4599 3243 Size: 998 358 6196 Fragments arranged by size: 6196 998 358 SalI G'TCGA_C Cuts at: 1936 4894 1936 Size: 2958 4594 SapI GCTCTTCn'nnn_ Cuts at: 3800 3800 Size: 7552 SbfI CC_TGCA'GG Cuts at: 4904 4904 Size: 7552 ScaI AGT'ACT Cuts at: 5800 7512 5800 Size: 1712 5840 SfcI C'TryA_G Cuts at: 826 1045 2907 3076 3579 4900 6035 6716 6907 Size: 219 1862 169 503 1321 1135 681 191 Cuts at: 6907 826 Size: 1471 Fragments arranged by size: 1862 1471 1321 1135 681 503 219 191 169 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7552 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7552 SmaI CCC'GGG Cuts at: 1872 3129 4055 4879 1872 Size: 1257 926 824 4545 Fragments arranged by size: 4545 1257 926 824 SmlI C'TyrA_G Cuts at: 820 1017 1943 3236 4490 5656 6557 6819 820 Size: 197 926 1293 1254 1166 901 262 1553 Fragments arranged by size: 1553 1293 1254 1166 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5343 493 Size: 4850 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7552 SphI G_CATG'C Cuts at: 1132 3124 4910 1132 Size: 1992 1786 3774 Fragments arranged by size: 3774 1992 1786 SspI AAT'ATT Cuts at: 5 52 3334 5476 5 Size: 47 3282 2142 2081 Fragments arranged by size: 3282 2142 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7552 StyI C'CwwG_G Cuts at: 513 1065 2004 2124 2554 2661 4182 4315 4991 Size: 552 939 120 430 107 1521 133 676 Cuts at: 4991 513 Size: 3074 Fragments arranged by size: 3074 1521 939 676 552 430 133 120 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2564 3701 4511 5579 5596 5749 5934 Size: 1255 984 1137 810 1068 17 153 185 Cuts at: 5934 6379 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1137 1068 984 810 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3325 4122 4834 Size: 274 80 33 51 1443 1348 797 712 Cuts at: 4834 5798 7510 96 Size: 964 1712 138 Fragments arranged by size: 1712 1443 1348 964 797 712 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2750 2782 3502 3534 4124 4156 4808 4840 7512 Size: 32 720 32 590 32 652 32 2672 Cuts at: 7512 7544 2750 Size: 32 2758 Fragments arranged by size: 2758 2672 720 652 590 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4010 1316 Size: 2694 4858 XbaI T'CTAG_A Cuts at: 4888 4888 Size: 7552 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2131 4432 2131 Size: 2301 5251 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7552 XmaI C'CCGG_G Cuts at: 1870 3127 4053 4877 1870 Size: 1257 926 824 4545 Fragments arranged by size: 4545 1257 926 824 XmnI GAAnn'nnTTC Cuts at: 3741 3818 5681 3741 Size: 77 1863 5612 Fragments arranged by size: 5612 1863 77 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5360 276 Size: 53 83 186 936 3826 2468 Fragments arranged by size: 3826 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI