(Circular) (Six-base) MAPSORT of: pFN21AB0449.seq Check: 204 from: 1 to: 6127 pFN21AB0449 6127 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2283 3937 278 Size: 53 83 186 936 747 1654 2468 Fragments arranged by size: 2468 1654 936 747 186 83 53 AccI GT'mk_AC Cuts at: 1937 3470 1937 Size: 1533 4594 Acc65I G'GTAC_C Cuts at: 3448 3448 Size: 6127 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2606 2615 2702 2822 2861 3356 4190 5574 Size: 974 9 87 120 39 495 834 1384 Cuts at: 5574 5842 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 974 834 495 268 120 87 39 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5930 AflIII A'CryG_T Cuts at: 1203 5026 6003 1203 Size: 3823 977 1327 Fragments arranged by size: 3823 1327 977 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 4856 4856 Size: 6127 AleI CACnn'nnGTG Cuts at: 1166 2654 1166 Size: 1488 4639 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2804 1184 Size: 1620 4507 AlwNI CAG_nnn'CTG Cuts at: 2692 2815 3257 3870 5442 2692 Size: 123 442 613 1572 3377 Fragments arranged by size: 3377 1572 613 442 123 ApaLI G'TGCA_C Cuts at: 3299 4184 5340 3299 Size: 885 1156 4086 Fragments arranged by size: 4086 1156 885 ApoI r'AATT_y Cuts at: 1493 2090 2327 3436 3662 3814 5923 1493 Size: 597 237 1109 226 152 2109 1697 Fragments arranged by size: 2109 1697 1109 597 237 226 152 AseI AT'TA_AT Cuts at: 160 1032 4681 160 Size: 872 3649 1606 Fragments arranged by size: 3649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 3028 3074 3452 1870 Size: 73 1085 46 378 4545 Fragments arranged by size: 4545 1085 378 73 46 BamHI G'GATC_C Cuts at: 2945 3457 3824 2945 Size: 512 367 5248 Fragments arranged by size: 5248 512 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2462 3448 4903 618 Size: 325 217 627 675 986 1455 1842 Fragments arranged by size: 1842 1455 986 675 627 325 217 BanII G_rGCy'C Cuts at: 1391 2357 3035 3446 1391 Size: 966 678 411 4072 Fragments arranged by size: 4072 966 678 411 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2470 3318 928 Size: 971 571 848 3737 Fragments arranged by size: 3737 971 848 571 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4318 4352 1903 Size: 34 2381 34 3678 Fragments arranged by size: 3678 2381 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3181 4019 5229 3181 Size: 838 1210 4079 Fragments arranged by size: 4079 1210 838 BclI T'GATC_A Cuts at: 1548 2007 1548 Size: 459 5668 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4738 136 Size: 107 122 71 631 605 3066 1525 Fragments arranged by size: 3066 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2359 6122 2359 Size: 3763 2364 BlpI GC'TnA_GC Cuts at: 2375 3544 2375 Size: 1169 4958 Bme1580I G_kGCm'C Cuts at: 1790 2058 2465 2883 3303 4188 5344 1790 Size: 268 407 418 420 885 1156 2573 Fragments arranged by size: 2573 1156 885 420 418 407 268 BmgBI CAC'GTC Cuts at: 1318 3096 1318 Size: 1778 4349 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2462 3858 4816 Size: 416 53 83 399 24 1045 1396 958 Cuts at: 4816 442 Size: 1753 Fragments arranged by size: 1753 1396 1045 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6127 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2313 2313 Size: 6127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2555 3335 4787 1239 Size: 1316 780 1452 2579 Fragments arranged by size: 2579 1452 1316 780 Bpu10I CC'TnA_GC Cuts at: 3209 3209 Size: 6127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4252 5117 5415 4252 Size: 865 298 4964 Fragments arranged by size: 4964 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2060 2154 3288 4790 882 Size: 617 561 94 1134 1502 2219 Fragments arranged by size: 2219 1502 1134 617 561 94 BsaAI yAC'GTr Cuts at: 493 1204 2437 3918 493 Size: 711 1233 1481 2702 Fragments arranged by size: 2702 1481 1233 711 BsaBI GATnn'nnATC Cuts at: 6121 6121 Size: 6127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2280 3934 4316 4951 Size: 53 83 186 936 747 1654 382 635 Cuts at: 4951 275 Size: 1451 Fragments arranged by size: 1654 1451 936 747 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3279 4560 4958 5232 5379 5790 3279 Size: 1281 398 274 147 411 3616 Fragments arranged by size: 3616 1281 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3282 3305 3312 3335 1178 Size: 30 165 30 1879 23 7 23 3970 Fragments arranged by size: 3970 1879 165 30 30 23 23 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2222 2558 3062 1186 Size: 1036 336 504 4251 Fragments arranged by size: 4251 1036 504 336 BseYI C'CCAG_C Cuts at: 2941 5330 5826 2941 Size: 2389 496 3242 Fragments arranged by size: 3242 2389 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2197 2255 2542 2774 3038 1163 Size: 573 461 58 287 232 264 4252 Fragments arranged by size: 4252 573 461 287 264 232 58 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3577 4338 5366 1540 Size: 275 186 1576 761 1028 2301 Fragments arranged by size: 2301 1576 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2541 3303 4188 4273 5344 2541 Size: 762 885 85 1071 3324 Fragments arranged by size: 3324 1071 885 762 85 BsmI GAATG_Cn' Cuts at: 3638 3731 3638 Size: 93 6034 BsmBI CGTCTCn'nnnn_ Cuts at: 3086 3086 Size: 6127 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2357 2465 2541 2883 3035 3303 Size: 399 268 299 108 76 342 152 268 Cuts at: 3303 3446 4188 4273 5344 1391 Size: 143 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 342 299 268 268 152 143 108 85 76 BspEI T'CCGG_A Cuts at: 5790 5790 Size: 6127 BspHI T'CATG_A Cuts at: 2010 2712 4016 5746 2010 Size: 702 1304 1730 2391 Fragments arranged by size: 2391 1730 1304 702 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2823 3482 844 Size: 1979 659 3489 Fragments arranged by size: 3489 1979 659 BsrBI CCG'CTC Cuts at: 1394 1823 2374 3163 4014 1394 Size: 429 551 789 851 3507 Fragments arranged by size: 3507 851 789 551 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4622 4796 65 Size: 1183 3374 174 1396 Fragments arranged by size: 3374 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2121 4771 4958 1700 Size: 110 311 2650 187 2869 Fragments arranged by size: 2869 2650 311 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6127 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6127 BssSI C'ACGA_G Cuts at: 2540 4187 5199 2540 Size: 1647 1012 3468 Fragments arranged by size: 3468 1647 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3257 794 Size: 2463 3664 BstBI TT'CG_AA Cuts at: 4931 4931 Size: 6127 BstEII G'GTnAC_C Cuts at: 1184 2908 1184 Size: 1724 4403 BstXI CCAn_nnnn'nTGG Cuts at: 2905 2905 Size: 6127 BstYI r'GATC_y Cuts at: 1972 2359 2526 2784 2945 3342 3457 3824 4209 Size: 387 167 258 161 397 115 367 385 Cuts at: 4209 4226 5678 5764 5776 6122 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 397 387 385 367 346 258 167 161 115 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2271 2304 513 Size: 552 1206 33 4336 Fragments arranged by size: 4336 1206 552 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5388 Fragments arranged by size: 5388 683 56 BtsI GCAGTG_nn' Cuts at: 2559 3315 3643 4437 4457 2559 Size: 756 328 794 20 4229 Fragments arranged by size: 4229 794 756 328 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6127 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2950 2985 529 Size: 35 2386 35 3671 Fragments arranged by size: 3671 2386 35 35 DraI TTT'AAA Cuts at: 3431 3431 Size: 6127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5134 809 Size: 507 3818 1802 Fragments arranged by size: 3818 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2847 2862 3006 Size: 54 1425 50 275 18 1017 15 144 Cuts at: 3006 3574 4463 6092 8 Size: 568 889 1629 43 Fragments arranged by size: 1629 1425 1017 889 568 275 144 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3574 1537 Size: 275 1762 4090 Fragments arranged by size: 4090 1762 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3029 3084 4057 1353 Size: 1676 55 973 3423 Fragments arranged by size: 3423 1676 973 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2140 2303 4714 5088 5234 387 Size: 1064 138 551 163 2411 374 146 1280 Fragments arranged by size: 2411 1280 1064 551 374 163 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2555 2606 2615 2702 2822 2861 3335 Size: 393 923 51 9 87 120 39 474 Cuts at: 3335 3356 4190 4787 5574 5842 1239 Size: 21 834 597 787 268 1524 Fragments arranged by size: 1524 923 834 787 597 474 393 268 120 87 51 39 21 9 EcoNI CCTnn'n_nnAGG Cuts at: 2321 2321 Size: 6127 EcoO109I rG'GnC_Cy Cuts at: 2758 2797 2925 3018 3078 3358 2758 Size: 39 128 93 60 280 5527 Fragments arranged by size: 5527 280 128 93 60 39 EcoRI G'AATT_C Cuts at: 2090 2327 3436 3814 2090 Size: 237 1109 378 4403 Fragments arranged by size: 4403 1109 378 237 EcoRV GAT'ATC Cuts at: 2311 2311 Size: 6127 FspI TGC'GCA Cuts at: 4633 4633 Size: 6127 HaeII r_GCGC'y Cuts at: 1678 2038 2263 2555 5274 1678 Size: 360 225 292 2719 2531 Fragments arranged by size: 2719 2531 360 292 225 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2344 2376 3305 3337 4768 Size: 32 135 32 772 32 929 32 1431 Cuts at: 4768 4800 4842 4874 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1431 929 772 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3471 3717 669 Size: 988 281 1533 246 3079 Fragments arranged by size: 3079 1533 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6127 HpaI GTT'AAC Cuts at: 3717 3717 Size: 6127 KpnI G_GTAC'C Cuts at: 3452 3452 Size: 6127 MfeI C'AATT_G Cuts at: 3726 3726 Size: 6127 MluI A'CGCG_T Cuts at: 6003 6003 Size: 6127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3165 5241 5425 1305 Size: 1860 2076 184 2007 Fragments arranged by size: 2076 2007 1860 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2864 3008 10 Size: 54 1425 343 1032 144 3129 Fragments arranged by size: 3129 1425 1032 343 144 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2654 2717 2972 4085 4444 4603 Size: 648 843 645 63 255 1113 359 159 Cuts at: 4603 518 Size: 2042 Fragments arranged by size: 2042 1113 843 648 645 359 255 159 63 MspA1I CmG'CkG Cuts at: 1606 2202 2245 2749 3543 4220 5368 5613 1606 Size: 596 43 504 794 677 1148 245 2120 Fragments arranged by size: 2120 1148 794 677 596 504 245 43 NcoI C'CATG_G Cuts at: 513 1065 2271 2304 513 Size: 552 1206 33 4336 Fragments arranged by size: 4336 1206 552 33 NdeI CA'TA_TG Cuts at: 387 387 Size: 6127 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6127 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 2386 2722 3247 3485 5030 1132 Size: 97 1157 336 525 238 1545 2229 Fragments arranged by size: 2229 1545 1157 525 336 238 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6127 PciI A'CATG_T Cuts at: 5026 5026 Size: 6127 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6127 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4196 4228 5709 5741 4196 Size: 32 1481 32 4582 Fragments arranged by size: 4582 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2797 3078 3358 2797 Size: 281 280 5566 Fragments arranged by size: 5566 281 280 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6127 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PstI C_TGCA'G Cuts at: 830 3479 830 Size: 2649 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 PvuII CAG'CTG Cuts at: 2749 2749 Size: 6127 SalI G'TCGA_C Cuts at: 1936 3469 1936 Size: 1533 4594 SanDI GG'GwC_CC Cuts at: 3078 3078 Size: 6127 SbfI CC_TGCA'GG Cuts at: 3479 3479 Size: 6127 ScaI AGT'ACT Cuts at: 2957 4375 6087 2957 Size: 1418 1712 2997 Fragments arranged by size: 2997 1712 1418 SfcI C'TryA_G Cuts at: 826 1045 2333 2342 3133 3475 4610 5291 5482 Size: 219 1288 9 791 342 1135 681 191 Cuts at: 5482 826 Size: 1471 Fragments arranged by size: 1471 1288 1135 791 681 342 219 191 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 3030 3454 1872 Size: 1158 424 4545 Fragments arranged by size: 4545 1158 424 SmlI C'TyrA_G Cuts at: 820 1017 1943 4231 5132 5394 820 Size: 197 926 2288 901 262 1553 Fragments arranged by size: 2288 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3918 493 Size: 3425 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6127 SphI G_CATG'C Cuts at: 1132 2722 3485 1132 Size: 1590 763 3774 Fragments arranged by size: 3774 1590 763 SspI AAT'ATT Cuts at: 5 52 2608 4051 5 Size: 47 2556 1443 2081 Fragments arranged by size: 2556 2081 1443 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6127 StyI C'CwwG_G Cuts at: 513 1065 2082 2271 2304 3566 513 Size: 552 1017 189 33 1262 3074 Fragments arranged by size: 3074 1262 1017 552 189 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2135 2631 4154 4171 4324 4509 4954 Size: 1255 555 496 1523 17 153 185 445 Cuts at: 4954 325 Size: 1498 Fragments arranged by size: 1523 1498 1255 555 496 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2955 4373 6085 Size: 274 80 33 51 1443 978 1418 1712 Cuts at: 6085 96 Size: 138 Fragments arranged by size: 1712 1443 1418 978 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3282 3314 6087 6119 3282 Size: 32 2773 32 3290 Fragments arranged by size: 3290 2773 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6127 XbaI T'CTAG_A Cuts at: 3463 3463 Size: 6127 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6127 XmaI C'CCGG_G Cuts at: 1870 3028 3452 1870 Size: 1158 424 4545 Fragments arranged by size: 4545 1158 424 XmnI GAAnn'nnTTC Cuts at: 4256 4256 Size: 6127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2281 3935 276 Size: 53 83 186 936 747 1654 2468 Fragments arranged by size: 2468 1654 936 747 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI