Restriction map for
pFN21AB0449
(Linear) (Six-base) MAPSORT of: KIBB0449.seq Check: 8322 from: 1 to: 1422
KIBB0449.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AatII G_ACGT'C
Cuts at: 0 278 1422
Size: 278 1144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 610 697 817 856 1351 1422
Size: 601 9 87 120 39 495 71
Fragments arranged by size:
601 495 120 87 71 39 9
AleI CACnn'nnGTG
Cuts at: 0 649 1422
Size: 649 773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 799 1422
Size: 799 623
AlwNI CAG_nnn'CTG
Cuts at: 0 687 810 1252 1422
Size: 687 123 442 170
Fragments arranged by size:
687 442 170 123
ApaLI G'TGCA_C
Cuts at: 0 1294 1422
Size: 1294 128
ApoI r'AATT_y
Cuts at: 0 85 322 1422
Size: 85 237 1100
Fragments arranged by size:
1100 237 85
AvaI C'yCGr_G
Cuts at: 0 1023 1069 1422
Size: 1023 46 353
Fragments arranged by size:
1023 353 46
BamHI G'GATC_C
Cuts at: 0 940 1422
Size: 940 482
BanI G'GyrC_C
Cuts at: 0 457 1422
Size: 457 965
BanII G_rGCy'C
Cuts at: 0 352 1030 1422
Size: 352 678 392
Fragments arranged by size:
678 392 352
BbsI GAAGACnn'nnnn_
Cuts at: 0 465 1313 1422
Size: 465 848 109
Fragments arranged by size:
848 465 109
BciVI GTATCCnnnnn_n'
Cuts at: 0 1176 1422
Size: 1176 246
BclI T'GATC_A
Cuts at: 0 2 1422
Size: 2 1420
BglII A'GATC_T
Cuts at: 0 354 1422
Size: 354 1068
BlpI GC'TnA_GC
Cuts at: 0 370 1422
Size: 370 1052
Bme1580I G_kGCm'C
Cuts at: 0 53 460 878 1298 1422
Size: 53 407 418 420 124
Fragments arranged by size:
420 418 407 124 53
BmgBI CAC'GTC
Cuts at: 0 1091 1422
Size: 1091 331
BmrI ACTGGGnnnn_n'
Cuts at: 0 457 1422
Size: 457 965
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 308 1422
Size: 308 1114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 1330 1422
Size: 550 780 92
Fragments arranged by size:
780 550 92
Bpu10I CC'TnA_GC
Cuts at: 0 1204 1422
Size: 1204 218
BsaI GGTCTCn'nnnn_
Cuts at: 0 55 149 1283 1422
Size: 55 94 1134 139
Fragments arranged by size:
1134 139 94 55
BsaAI yAC'GTr
Cuts at: 0 432 1422
Size: 432 990
BsaHI Gr'CG_yC
Cuts at: 0 275 1422
Size: 275 1147
BsaWI w'CCGG_w
Cuts at: 0 1274 1422
Size: 1274 148
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1277 1300 1307 1330 1422
Size: 1277 23 7 23 92
Fragments arranged by size:
1277 92 23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 553 1057 1422
Size: 217 336 504 365
Fragments arranged by size:
504 365 336 217
BseYI C'CCAG_C
Cuts at: 0 936 1422
Size: 936 486
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 250 537 769 1033 1422
Size: 192 58 287 232 264 389
Fragments arranged by size:
389 287 264 232 192 58
BsiHKAI G_wGCw'C
Cuts at: 0 536 1298 1422
Size: 536 762 124
Fragments arranged by size:
762 536 124
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1081 1422
Size: 1081 341
Bsp1286I G_dGCh'C
Cuts at: 0 53 352 460 536 878 1030 1298 1422
Size: 53 299 108 76 342 152 268 124
Fragments arranged by size:
342 299 268 152 124 108 76 53
BspHI T'CATG_A
Cuts at: 0 5 707 1422
Size: 5 702 715
Fragments arranged by size:
715 702 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 818 1422
Size: 818 604
BsrBI CCG'CTC
Cuts at: 0 369 1158 1422
Size: 369 789 264
Fragments arranged by size:
789 369 264
BsrFI r'CCGG_y
Cuts at: 0 116 1422
Size: 116 1306
BssSI C'ACGA_G
Cuts at: 0 535 1422
Size: 535 887
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1252 1422
Size: 1252 170
BstEII G'GTnAC_C
Cuts at: 0 903 1422
Size: 903 519
BstXI CCAn_nnnn'nTGG
Cuts at: 0 900 1422
Size: 900 522
BstYI r'GATC_y
Cuts at: 0 354 521 779 940 1337 1422
Size: 354 167 258 161 397 85
Fragments arranged by size:
397 354 258 167 161 85
BtgI C'CryG_G
Cuts at: 0 266 299 1422
Size: 266 33 1123
Fragments arranged by size:
1123 266 33
BtsI GCAGTG_nn'
Cuts at: 0 554 1310 1422
Size: 554 756 112
Fragments arranged by size:
756 554 112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 945 980 1422
Size: 945 35 442
Fragments arranged by size:
945 442 35
EaeI y'GGCC_r
Cuts at: 0 842 857 1001 1422
Size: 842 15 144 421
Fragments arranged by size:
842 421 144 15
EarI CTCTTCn'nnn_
Cuts at: 0 1024 1079 1422
Size: 1024 55 343
Fragments arranged by size:
1024 343 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 135 298 1422
Size: 135 163 1124
Fragments arranged by size:
1124 163 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 601 610 697 817 856 1330 1351
Size: 550 51 9 87 120 39 474 21
Cuts at: 1351 1422
Size: 71
Fragments arranged by size:
550 474 120 87 71 51 39 21
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 316 1422
Size: 316 1106
EcoO109I rG'GnC_Cy
Cuts at: 0 753 792 920 1013 1073 1353 1422
Size: 753 39 128 93 60 280 69
Fragments arranged by size:
753 280 128 93 69 60 39
EcoRI G'AATT_C
Cuts at: 0 85 322 1422
Size: 85 237 1100
Fragments arranged by size:
1100 237 85
EcoRV GAT'ATC
Cuts at: 0 306 1422
Size: 306 1116
HaeII r_GCGC'y
Cuts at: 0 33 258 550 1422
Size: 33 225 292 872
Fragments arranged by size:
872 292 225 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 339 371 1300 1332 1422
Size: 339 32 929 32 90
Fragments arranged by size:
929 339 90 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1160 1422
Size: 1160 262
MscI TGG'CCA
Cuts at: 0 859 1003 1422
Size: 859 144 419
Fragments arranged by size:
859 419 144
MslI CAynn'nnrTG
Cuts at: 0 649 712 967 1422
Size: 649 63 255 455
Fragments arranged by size:
649 455 255 63
MspA1I CmG'CkG
Cuts at: 0 197 240 744 1422
Size: 197 43 504 678
Fragments arranged by size:
678 504 197 43
NcoI C'CATG_G
Cuts at: 0 266 299 1422
Size: 266 33 1123
Fragments arranged by size:
1123 266 33
NspI r_CATG'y
Cuts at: 0 381 717 1242 1422
Size: 381 336 525 180
Fragments arranged by size:
525 381 336 180
PpuMI rG'GwC_Cy
Cuts at: 0 792 1073 1353 1422
Size: 792 281 280 69
Fragments arranged by size:
792 281 280 69
PvuII CAG'CTG
Cuts at: 0 744 1422
Size: 744 678
SanDI GG'GwC_CC
Cuts at: 0 1073 1422
Size: 1073 349
ScaI AGT'ACT
Cuts at: 0 952 1422
Size: 952 470
SfcI C'TryA_G
Cuts at: 0 328 337 1128 1422
Size: 328 9 791 294
Fragments arranged by size:
791 328 294 9
SmaI CCC'GGG
Cuts at: 0 1025 1422
Size: 1025 397
SphI G_CATG'C
Cuts at: 0 717 1422
Size: 717 705
SspI AAT'ATT
Cuts at: 0 603 1422
Size: 603 819
StyI C'CwwG_G
Cuts at: 0 77 266 299 1422
Size: 77 189 33 1123
Fragments arranged by size:
1123 189 77 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 130 626 1422
Size: 130 496 796
Fragments arranged by size:
796 496 130
TatI w'GTAC_w
Cuts at: 0 950 1422
Size: 950 472
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1277 1309 1422
Size: 1277 32 113
Fragments arranged by size:
1277 113 32
XmaI C'CCGG_G
Cuts at: 0 1023 1422
Size: 1023 399
ZraI GAC'GTC
Cuts at: 0 276 1422
Size: 276 1146
Enzymes that do cut:
AatII AcuI AleI AlfI AlwNI ApaLI ApoI AvaI
BamHI BanI BanII BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstAPI BstEII
BstXI BstYI BtgI BtsI CspCI EaeI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I MmeI
MscI MslI MspA1I NcoI NspI PpuMI PvuII SanDI
ScaI SfcI SmaI SphI SspI StyI TaqII TatI
TstI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BfrBI BglI BmtI BpuEI BsaBI
BsiEI BsiWI BsmI BspEI BsrDI BsrGI BssHII BstBI
BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI
EcoICRI FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SrfI StuI SwaI Tth111I XbaI XcmI XhoI
XmnI