(Circular) (Six-base) MAPSORT of: pFN21AB0442.seq Check: 6794 from: 1 to: 7411 pFN21AB0442 7411 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4345 4345 Size: 7411 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5221 278 Size: 53 83 186 936 3685 2468 Fragments arranged by size: 3685 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3996 4754 1937 Size: 2059 758 4594 Fragments arranged by size: 4594 2059 758 Acc65I G'GTAC_C Cuts at: 4313 4732 4313 Size: 419 6992 AclI AA'CG_TT Cuts at: 1563 5538 5911 1563 Size: 3975 373 3063 Fragments arranged by size: 3975 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2488 2823 3181 3605 4095 5474 6858 7126 Size: 856 335 358 424 490 1379 1384 268 Cuts at: 7126 1632 Size: 1917 Fragments arranged by size: 1917 1384 1379 856 490 424 358 335 268 AfeI AGC'GCT Cuts at: 2394 3743 2394 Size: 1349 6062 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7214 AflIII A'CryG_T Cuts at: 1203 3647 3649 3673 6310 7287 1203 Size: 2444 2 24 2637 977 1327 Fragments arranged by size: 2637 2444 1327 977 24 2 AgeI A'CCGG_T Cuts at: 6242 6242 Size: 7411 AhdI GACnn_n'nnGTC Cuts at: 6140 6140 Size: 7411 AleI CACnn'nnGTG Cuts at: 1166 3518 1166 Size: 2352 5059 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7411 AlwNI CAG_nnn'CTG Cuts at: 3757 3789 5154 6726 3757 Size: 32 1365 1572 4442 Fragments arranged by size: 4442 1572 1365 32 ApaI G_GGCC'C Cuts at: 2769 2965 3298 4266 2769 Size: 196 333 968 5914 Fragments arranged by size: 5914 968 333 196 ApaLI G'TGCA_C Cuts at: 3455 5468 6624 3455 Size: 2013 1156 4242 Fragments arranged by size: 4242 2013 1156 ApoI r'AATT_y Cuts at: 1493 2039 3595 4720 4946 5098 7207 1493 Size: 546 1556 1125 226 152 2109 1697 Fragments arranged by size: 2109 1697 1556 1125 546 226 152 AseI AT'TA_AT Cuts at: 160 1032 5965 160 Size: 872 4933 1606 Fragments arranged by size: 4933 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7411 AvaI C'yCGr_G Cuts at: 1870 1943 2336 2658 2824 2922 2929 3956 4736 Size: 73 393 322 166 98 7 1027 780 Cuts at: 4736 1870 Size: 4545 Fragments arranged by size: 4545 1027 780 393 322 166 98 73 7 AvrII C'CTAG_G Cuts at: 3367 3367 Size: 7411 BamHI G'GATC_C Cuts at: 4741 5108 4741 Size: 367 7044 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2252 3512 3721 4313 4732 Size: 325 217 627 465 1260 209 592 419 Cuts at: 4732 6187 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1260 627 592 465 419 325 217 209 BanII G_rGCy'C Cuts at: 1391 2157 2642 2769 2936 2965 3286 3298 4080 Size: 766 485 127 167 29 321 12 782 Cuts at: 4080 4266 4394 4564 4730 1391 Size: 186 128 170 166 4072 Fragments arranged by size: 4072 782 766 485 321 186 170 167 166 128 127 29 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2996 4194 928 Size: 971 1097 1198 4145 Fragments arranged by size: 4145 1198 1097 971 BbvCI CC'TCA_GC Cuts at: 3260 4515 3260 Size: 1255 6156 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5602 5636 1903 Size: 34 3665 34 3678 Fragments arranged by size: 3678 3665 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4514 5303 6513 4514 Size: 789 1210 5412 Fragments arranged by size: 5412 1210 789 BclI T'GATC_A Cuts at: 1548 1548 Size: 7411 BfrBI ATG'CAT Cuts at: 2218 2218 Size: 7411 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2721 6022 Size: 107 122 71 631 605 337 712 3301 Cuts at: 6022 136 Size: 1525 Fragments arranged by size: 3301 1525 712 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 7406 7406 Size: 7411 BlpI GC'TnA_GC Cuts at: 3745 4828 3745 Size: 1083 6328 Bme1580I G_kGCm'C Cuts at: 1790 2769 2965 3147 3298 3459 3681 3726 4266 Size: 979 196 182 151 161 222 45 540 Cuts at: 4266 4330 5472 6628 1790 Size: 64 1142 1156 2573 Fragments arranged by size: 2573 1156 1142 979 540 222 196 182 161 151 64 45 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7411 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3777 5142 6100 Size: 416 53 83 399 24 2360 1365 958 Cuts at: 6100 442 Size: 1753 Fragments arranged by size: 2360 1753 1365 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7411 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2638 3000 3064 2638 Size: 362 64 6985 Fragments arranged by size: 6985 362 64 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2417 2716 3081 3275 4658 4713 6071 1239 Size: 1178 299 365 194 1383 55 1358 2579 Fragments arranged by size: 2579 1383 1358 1178 365 299 194 55 Bpu10I CC'TnA_GC Cuts at: 3260 4515 3260 Size: 1255 6156 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5536 6401 6699 5536 Size: 865 298 6248 Fragments arranged by size: 6248 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4659 6074 882 Size: 617 3160 1415 2219 Fragments arranged by size: 3160 2219 1415 617 BsaAI yAC'GTr Cuts at: 493 1204 3650 3676 5202 493 Size: 711 2446 26 1526 2702 Fragments arranged by size: 2702 2446 1526 711 26 BsaBI GATnn'nnATC Cuts at: 3404 4500 7405 3404 Size: 1096 2905 3410 Fragments arranged by size: 3410 2905 1096 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5218 5600 6235 275 Size: 53 83 186 936 3685 382 635 1451 Fragments arranged by size: 3685 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4084 4564 5844 6242 6516 6663 7074 4084 Size: 480 1280 398 274 147 411 4421 Fragments arranged by size: 4421 1280 480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2918 2948 1178 Size: 30 165 30 1515 30 5641 Fragments arranged by size: 5641 1515 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2741 3053 3084 3095 3816 3911 3914 Size: 859 696 312 31 11 721 95 3 Cuts at: 3914 4088 4384 4437 1186 Size: 174 296 53 4160 Fragments arranged by size: 4160 859 721 696 312 296 174 95 53 31 11 3 BseYI C'CCAG_C Cuts at: 2080 2287 2309 2318 2345 2773 2848 3236 3738 Size: 207 22 9 27 428 75 388 502 Cuts at: 3738 6614 7110 2080 Size: 2876 496 2381 Fragments arranged by size: 2876 2381 502 496 428 388 207 75 27 22 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2157 4668 1163 Size: 573 421 2511 3906 Fragments arranged by size: 3906 2511 573 421 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4442 4861 5622 6650 1540 Size: 275 186 2441 419 761 1028 2301 Fragments arranged by size: 2441 2301 1028 761 419 275 186 BsiHKAI G_wGCw'C Cuts at: 2761 3459 3737 5472 5557 6628 2761 Size: 698 278 1735 85 1071 3544 Fragments arranged by size: 3544 1735 1071 698 278 85 BsmI GAATG_Cn' Cuts at: 2218 4922 5015 2218 Size: 2704 93 4614 Fragments arranged by size: 4614 2704 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3617 3706 3617 Size: 89 7322 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2642 2761 2769 2936 2965 3147 Size: 399 367 485 119 8 167 29 182 Cuts at: 3147 3286 3298 3459 3681 3726 3737 4080 4266 Size: 139 12 161 222 45 11 343 186 Cuts at: 4266 4330 4394 4564 4730 5472 5557 6628 1391 Size: 64 64 170 166 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 485 399 367 343 222 186 182 170 167 166 161 139 119 85 64 64 45 29 12 11 8 BspEI T'CCGG_A Cuts at: 4564 7074 4564 Size: 2510 4901 BspHI T'CATG_A Cuts at: 5300 7030 5300 Size: 1730 5681 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3438 4345 4519 4766 844 Size: 2594 907 174 247 3489 Fragments arranged by size: 3489 2594 907 247 174 BsrBI CCG'CTC Cuts at: 1394 1823 5298 1394 Size: 429 3475 3507 Fragments arranged by size: 3507 3475 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2909 3061 3896 5906 6080 65 Size: 1183 1661 152 835 2010 174 1396 Fragments arranged by size: 2010 1661 1396 1183 835 174 152 BsrFI r'CCGG_y Cuts at: 1700 1810 6055 6242 1700 Size: 110 4245 187 2869 Fragments arranged by size: 4245 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7411 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7411 BssSI C'ACGA_G Cuts at: 2536 5471 6483 2536 Size: 2935 1012 3464 Fragments arranged by size: 3464 2935 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7411 BstBI TT'CG_AA Cuts at: 6215 6215 Size: 7411 BstEII G'GTnAC_C Cuts at: 1184 3347 1184 Size: 2163 5248 BstXI CCAn_nnnn'nTGG Cuts at: 3159 3225 3523 3159 Size: 66 298 7047 Fragments arranged by size: 7047 298 66 BstYI r'GATC_y Cuts at: 1972 2689 4741 5108 5493 5510 6962 7048 7060 Size: 717 2052 367 385 17 1452 86 12 Cuts at: 7060 7406 1972 Size: 346 1977 Fragments arranged by size: 2052 1977 1452 717 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2675 2783 2863 3319 3483 513 Size: 552 939 671 108 80 456 164 4441 Fragments arranged by size: 4441 939 671 552 456 164 108 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6672 Fragments arranged by size: 6672 683 56 BtsI GCAGTG_nn' Cuts at: 3024 3147 3411 4927 5721 5741 3024 Size: 123 264 1516 794 20 4694 Fragments arranged by size: 4694 1516 794 264 123 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7411 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2892 2927 529 Size: 35 2328 35 5013 Fragments arranged by size: 5013 2328 35 35 DraI TTT'AAA Cuts at: 4715 4715 Size: 7411 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6418 809 Size: 507 5102 1802 Fragments arranged by size: 5102 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2326 2672 Size: 54 1425 50 275 18 183 313 346 Cuts at: 2672 3480 3486 3558 3983 4439 4660 4858 5747 Size: 808 6 72 425 456 221 198 889 Cuts at: 5747 7376 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 808 456 425 346 313 275 221 198 183 72 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 4439 4858 1537 Size: 275 2627 419 4090 Fragments arranged by size: 4090 2627 419 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2874 4455 4692 5341 1353 Size: 1521 1581 237 649 3423 Fragments arranged by size: 3423 1581 1521 649 237 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3013 4033 4413 5998 6372 6518 Size: 1064 138 1424 1020 380 1585 374 146 Cuts at: 6518 387 Size: 1280 Fragments arranged by size: 1585 1424 1280 1064 1020 380 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2417 2488 2716 2823 3081 3181 3275 Size: 393 785 71 228 107 258 100 94 Cuts at: 3275 3605 4095 4658 4713 5474 6071 6858 7126 Size: 330 490 563 55 761 597 787 268 Cuts at: 7126 1239 Size: 1524 Fragments arranged by size: 1524 787 785 761 597 563 490 393 330 268 258 228 107 100 94 71 55 EcoNI CCTnn'n_nnAGG Cuts at: 3099 3099 Size: 7411 EcoO109I rG'GnC_Cy Cuts at: 2071 2496 2926 2961 2962 3201 3872 4161 4262 Size: 425 430 35 1 239 671 289 101 Cuts at: 4262 4263 4475 2071 Size: 1 212 5007 Fragments arranged by size: 5007 671 430 425 289 239 212 101 35 1 1 EcoRI G'AATT_C Cuts at: 3595 4720 5098 3595 Size: 1125 378 5908 Fragments arranged by size: 5908 1125 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3085 3085 Size: 7411 FspI TGC'GCA Cuts at: 5917 5917 Size: 7411 HaeII r_GCGC'y Cuts at: 1678 2172 2396 2815 3745 6558 1678 Size: 494 224 419 930 2813 2531 Fragments arranged by size: 2813 2531 930 494 419 224 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2585 2617 2918 2950 4599 Size: 32 135 32 1013 32 301 32 1649 Cuts at: 4599 4631 6052 6084 6126 6158 1373 Size: 32 1421 32 42 32 2626 Fragments arranged by size: 2626 1649 1421 1013 301 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4755 5001 669 Size: 988 281 2817 246 3079 Fragments arranged by size: 3079 2817 988 281 246 HindIII A'AGCT_T Cuts at: 748 3587 748 Size: 2839 4572 HpaI GTT'AAC Cuts at: 5001 5001 Size: 7411 KpnI G_GTAC'C Cuts at: 4317 4736 4317 Size: 419 6992 MfeI C'AATT_G Cuts at: 5010 5010 Size: 7411 MluI A'CGCG_T Cuts at: 7287 7287 Size: 7411 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3504 3855 6525 6709 1305 Size: 2199 351 2670 184 2007 Fragments arranged by size: 2670 2199 2007 351 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3482 10 Size: 54 1425 343 1650 3939 Fragments arranged by size: 3939 1650 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3518 4139 5369 5728 5887 518 Size: 648 2352 621 1230 359 159 2042 Fragments arranged by size: 2352 2042 1230 648 621 359 159 MspA1I CmG'CkG Cuts at: 1606 2019 2080 2647 2785 2972 3253 4599 4827 Size: 413 61 567 138 187 281 1346 228 Cuts at: 4827 5504 6652 6897 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1346 1148 677 567 413 281 245 228 187 138 61 NcoI C'CATG_G Cuts at: 513 1065 2004 2675 513 Size: 552 939 671 5249 Fragments arranged by size: 5249 939 671 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7411 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7411 NotI GC'GGCC_GC Cuts at: 4858 4858 Size: 7411 NsiI A_TGCA'T Cuts at: 2220 2220 Size: 7411 NspI r_CATG'y Cuts at: 1132 1229 2218 4240 4769 6314 1132 Size: 97 989 2022 529 1545 2229 Fragments arranged by size: 2229 2022 1545 989 529 97 PasI CC'CwG_GG Cuts at: 1348 3682 4405 4550 1348 Size: 2334 723 145 4209 Fragments arranged by size: 4209 2334 723 145 PciI A'CATG_T Cuts at: 6310 6310 Size: 7411 PflMI CCAn_nnn'nTGG Cuts at: 1108 2516 4544 1108 Size: 1408 2028 3975 Fragments arranged by size: 3975 2028 1408 PfoI T'CCnGG_A Cuts at: 3097 3107 4690 3097 Size: 10 1583 5818 Fragments arranged by size: 5818 1583 10 PmeI GTTT'AAAC Cuts at: 4715 4715 Size: 7411 PmlI CAC'GTG Cuts at: 3650 3676 3650 Size: 26 7385 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5480 5512 6993 7025 5480 Size: 32 1481 32 5866 Fragments arranged by size: 5866 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2926 3201 3872 2926 Size: 275 671 6465 Fragments arranged by size: 6465 671 275 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7411 PsiI TTA'TAA Cuts at: 4981 4981 Size: 7411 PspOMI G'GGCC_C Cuts at: 2765 2961 3294 4262 2765 Size: 196 333 968 5914 Fragments arranged by size: 5914 968 333 196 PstI C_TGCA'G Cuts at: 830 3418 3924 4342 4763 830 Size: 2588 506 418 421 3478 Fragments arranged by size: 3478 2588 506 421 418 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7411 PvuII CAG'CTG Cuts at: 2019 2972 3253 2019 Size: 953 281 6177 Fragments arranged by size: 6177 953 281 SacII CC_GC'GG Cuts at: 2786 2786 Size: 7411 SalI G'TCGA_C Cuts at: 1936 4753 1936 Size: 2817 4594 SanDI GG'GwC_CC Cuts at: 2926 2926 Size: 7411 SapI GCTCTTCn'nnn_ Cuts at: 4692 4692 Size: 7411 SbfI CC_TGCA'GG Cuts at: 4763 4763 Size: 7411 ScaI AGT'ACT Cuts at: 5659 7371 5659 Size: 1712 5699 SfcI C'TryA_G Cuts at: 826 1045 2467 3373 3395 3414 3775 3920 4276 Size: 219 1422 906 22 19 361 145 356 Cuts at: 4276 4338 4591 4759 5894 6575 6766 826 Size: 62 253 168 1135 681 191 1471 Fragments arranged by size: 1471 1422 1135 906 681 361 356 253 219 191 168 145 62 22 19 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7411 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7411 SmaI CCC'GGG Cuts at: 1872 2660 2924 2931 4738 1872 Size: 788 264 7 1807 4545 Fragments arranged by size: 4545 1807 788 264 7 SmlI C'TyrA_G Cuts at: 820 1017 1943 2336 5515 6416 6678 820 Size: 197 926 393 3179 901 262 1553 Fragments arranged by size: 3179 1553 926 901 393 262 197 SnaBI TAC'GTA Cuts at: 493 5202 493 Size: 4709 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7411 SphI G_CATG'C Cuts at: 1132 2218 4240 4769 1132 Size: 1086 2022 529 3774 Fragments arranged by size: 3774 2022 1086 529 SspI AAT'ATT Cuts at: 5 52 5335 5 Size: 47 5283 2081 Fragments arranged by size: 5283 2081 47 StuI AGG'CCT Cuts at: 1517 3773 1517 Size: 2256 5155 StyI C'CwwG_G Cuts at: 513 1065 2004 2145 2675 3069 3165 3367 3516 Size: 552 939 141 530 394 96 202 149 Cuts at: 3516 3849 4850 513 Size: 333 1001 3074 Fragments arranged by size: 3074 1001 939 552 530 394 333 202 149 141 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3021 4171 5438 5455 5608 5793 6238 Size: 1255 1441 1150 1267 17 153 185 445 Cuts at: 6238 325 Size: 1498 Fragments arranged by size: 1498 1441 1267 1255 1150 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3706 3960 5657 Size: 274 80 33 51 1443 1729 254 1697 Cuts at: 5657 7369 96 Size: 1712 138 Fragments arranged by size: 1729 1712 1697 1443 274 254 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7371 7403 7371 Size: 32 7379 Tth111I GACn'n_nGTC Cuts at: 1316 4613 1316 Size: 3297 4114 XbaI T'CTAG_A Cuts at: 4747 4747 Size: 7411 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2739 2739 Size: 7411 XhoI C'TCGA_G Cuts at: 1943 2336 1943 Size: 393 7018 XmaI C'CCGG_G Cuts at: 1870 2658 2922 2929 4736 1870 Size: 788 264 7 1807 4545 Fragments arranged by size: 4545 1807 788 264 7 XmnI GAAnn'nnTTC Cuts at: 5540 5540 Size: 7411 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5219 276 Size: 53 83 186 936 3685 2468 Fragments arranged by size: 3685 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacI SexAI SfiI SfoI SrfI SwaI