Restriction map for
pFN21AB6459
(Linear) (Six-base) MAPSORT of: KIBB6459.seq Check: 8622 from: 1 to: 954
KIBB6459.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 453 954
Size: 453 501
AlwNI CAG_nnn'CTG
Cuts at: 0 469 954
Size: 469 485
ApoI r'AATT_y
Cuts at: 0 350 383 502 768 954
Size: 350 33 119 266 186
Fragments arranged by size:
350 266 186 119 33
AseI AT'TA_AT
Cuts at: 0 481 676 954
Size: 481 195 278
Fragments arranged by size:
481 278 195
BanII G_rGCy'C
Cuts at: 0 189 954
Size: 189 765
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 811 845 954
Size: 811 34 109
Fragments arranged by size:
811 109 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 479 954
Size: 479 475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 856 954
Size: 79 777 98
Fragments arranged by size:
777 98 79
BsaBI GATnn'nnATC
Cuts at: 0 908 954
Size: 908 46
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 954
Size: 250 704
BseYI C'CCAG_C
Cuts at: 0 588 954
Size: 588 366
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 954
Size: 482 472
BsiHKAI G_wGCw'C
Cuts at: 0 77 189 601 954
Size: 77 112 412 353
Fragments arranged by size:
412 353 112 77
Bsp1286I G_dGCh'C
Cuts at: 0 77 189 601 954
Size: 77 112 412 353
Fragments arranged by size:
412 353 112 77
BspHI T'CATG_A
Cuts at: 0 215 498 954
Size: 215 283 456
Fragments arranged by size:
456 283 215
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 168 954
Size: 168 786
BssSI C'ACGA_G
Cuts at: 0 230 954
Size: 230 724
BstYI r'GATC_y
Cuts at: 0 903 954
Size: 903 51
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 439 474 954
Size: 439 35 480
Fragments arranged by size:
480 439 35
DraI TTT'AAA
Cuts at: 0 854 954
Size: 854 100
EaeI y'GGCC_r
Cuts at: 0 487 954
Size: 487 467
EcoICRI GAG'CTC
Cuts at: 0 187 954
Size: 187 767
EcoRV GAT'ATC
Cuts at: 0 931 954
Size: 931 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 213 245 439 471 954
Size: 213 32 194 32 483
Fragments arranged by size:
483 213 194 32 32
HindIII A'AGCT_T
Cuts at: 0 178 412 954
Size: 178 234 542
Fragments arranged by size:
542 234 178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 954
Size: 611 343
MslI CAynn'nnrTG
Cuts at: 0 220 745 954
Size: 220 525 209
Fragments arranged by size:
525 220 209
PasI CC'CwG_GG
Cuts at: 0 398 954
Size: 398 556
PflMI CCAn_nnn'nTGG
Cuts at: 0 790 954
Size: 790 164
PsiI TTA'TAA
Cuts at: 0 614 954
Size: 614 340
PstI C_TGCA'G
Cuts at: 0 165 954
Size: 165 789
SacI G_AGCT'C
Cuts at: 0 189 954
Size: 189 765
SfcI C'TryA_G
Cuts at: 0 161 954
Size: 161 793
SmlI C'TyrA_G
Cuts at: 0 94 871 954
Size: 94 777 83
Fragments arranged by size:
777 94 83
SspI AAT'ATT
Cuts at: 0 919 954
Size: 919 35
StuI AGG'CCT
Cuts at: 0 807 954
Size: 807 147
StyI C'CwwG_G
Cuts at: 0 664 954
Size: 664 290
TatI w'GTAC_w
Cuts at: 0 371 654 954
Size: 371 283 300
Fragments arranged by size:
371 300 283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 133 954
Size: 133 821
XmnI GAAnn'nnTTC
Cuts at: 0 714 954
Size: 714 240
Enzymes that do cut:
AhdI AlwNI ApoI AseI BanII BcgI BmrI BpuEI
BsaBI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI
BssSI BstYI CspCI DraI EaeI EcoICRI EcoRV Hin4I
HindIII MmeI MslI PasI PflMI PsiI PstI SacI
SfcI SmlI SspI StuI StyI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraIII DrdI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MscI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SwaI TaqII TstI Tth111I
XbaI XhoI XmaI ZraI