(Circular) (Six-base) MAPSORT of: pFN21AB0435.seq Check: 6581 from: 1 to: 6049 pFN21AB0435 6049 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3331 3331 Size: 6049 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3859 278 Size: 53 83 186 936 2323 2468 Fragments arranged by size: 2468 2323 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2856 3392 1937 Size: 919 536 4594 Fragments arranged by size: 4594 919 536 Acc65I G'GTAC_C Cuts at: 3370 3370 Size: 6049 AclI AA'CG_TT Cuts at: 1563 4176 4549 1563 Size: 2613 373 3063 Fragments arranged by size: 3063 2613 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2445 2657 3173 4112 5496 5764 1632 Size: 813 212 516 939 1384 268 1917 Fragments arranged by size: 1917 1384 939 813 516 268 212 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5852 AflIII A'CryG_T Cuts at: 1203 3009 4948 5925 1203 Size: 1806 1939 977 1327 Fragments arranged by size: 1939 1806 1327 977 AgeI A'CCGG_T Cuts at: 4880 4880 Size: 6049 AhdI GACnn_n'nnGTC Cuts at: 4778 4778 Size: 6049 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6049 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6049 AlwNI CAG_nnn'CTG Cuts at: 2842 3792 5364 2842 Size: 950 1572 3527 Fragments arranged by size: 3527 1572 950 ApaLI G'TGCA_C Cuts at: 4106 5262 4106 Size: 1156 4893 ApoI r'AATT_y Cuts at: 1493 2765 3358 3584 3736 5845 1493 Size: 1272 593 226 152 2109 1697 Fragments arranged by size: 2109 1697 1272 593 226 152 AseI AT'TA_AT Cuts at: 160 1032 4603 160 Size: 872 3571 1606 Fragments arranged by size: 3571 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 AvaI C'yCGr_G Cuts at: 1870 1943 2076 2491 2677 3062 3374 1870 Size: 73 133 415 186 385 312 4545 Fragments arranged by size: 4545 415 385 312 186 133 73 BamHI G'GATC_C Cuts at: 3379 3746 3379 Size: 367 5682 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2192 2425 2486 3126 3370 Size: 325 217 627 405 233 61 640 244 Cuts at: 3370 4825 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 640 627 405 325 244 233 217 61 BanII G_rGCy'C Cuts at: 1391 2172 2874 2894 3368 1391 Size: 781 702 20 474 4072 Fragments arranged by size: 4072 781 702 474 20 BbeI G_GCGC'C Cuts at: 3130 3130 Size: 6049 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2645 3074 928 Size: 971 746 429 3903 Fragments arranged by size: 3903 971 746 429 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2798 2832 2984 3018 4240 4274 1903 Size: 34 861 34 152 34 1222 34 3678 Fragments arranged by size: 3678 1222 861 152 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3941 5151 3941 Size: 1210 4839 BclI T'GATC_A Cuts at: 1548 2568 1548 Size: 1020 5029 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2358 4660 136 Size: 107 122 71 631 605 686 2302 1525 Fragments arranged by size: 2302 1525 686 631 605 122 107 71 BglII A'GATC_T Cuts at: 6044 6044 Size: 6049 BlpI GC'TnA_GC Cuts at: 2071 2843 3083 3466 2071 Size: 772 240 383 4654 Fragments arranged by size: 4654 772 383 240 Bme1580I G_kGCm'C Cuts at: 1790 2147 4110 5266 1790 Size: 357 1963 1156 2573 Fragments arranged by size: 2573 1963 1156 357 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6049 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3780 4738 442 Size: 416 53 83 399 24 2363 958 1753 Fragments arranged by size: 2363 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6049 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2393 2772 3320 4709 1239 Size: 1154 379 548 1389 2579 Fragments arranged by size: 2579 1389 1154 548 379 Bpu10I CC'TnA_GC Cuts at: 3231 3231 Size: 6049 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4174 5039 5337 4174 Size: 865 298 4886 Fragments arranged by size: 4886 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4712 882 Size: 617 3213 2219 Fragments arranged by size: 3213 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3093 3840 493 Size: 711 1889 747 2702 Fragments arranged by size: 2702 1889 747 711 BsaBI GATnn'nnATC Cuts at: 6043 6043 Size: 6049 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2033 2269 3127 3856 Size: 53 83 186 936 500 236 858 729 Cuts at: 3856 4238 4873 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 858 729 635 500 382 236 186 83 53 BsaWI w'CCGG_w Cuts at: 4482 4880 5154 5301 5712 4482 Size: 398 274 147 411 4819 Fragments arranged by size: 4819 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2494 2524 2770 2800 3269 Size: 30 165 30 1091 30 246 30 469 Cuts at: 3269 3299 1178 Size: 30 3928 Fragments arranged by size: 3928 1091 469 246 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2180 2729 2882 1186 Size: 994 549 153 4353 Fragments arranged by size: 4353 994 549 153 BseYI C'CCAG_C Cuts at: 2656 3330 5252 5748 2656 Size: 674 1922 496 2957 Fragments arranged by size: 2957 1922 674 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2008 3272 3309 1163 Size: 573 272 1264 37 3903 Fragments arranged by size: 3903 1264 573 272 37 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3499 4260 5288 1540 Size: 275 186 1498 761 1028 2301 Fragments arranged by size: 2301 1498 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2072 4110 4195 5266 2072 Size: 2038 85 1071 2855 Fragments arranged by size: 2855 2038 1071 85 BsmI GAATG_Cn' Cuts at: 3084 3560 3653 3084 Size: 476 93 5480 Fragments arranged by size: 5480 476 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2147 2172 2874 2894 3368 4110 Size: 399 282 75 25 702 20 474 742 Cuts at: 4110 4195 5266 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 702 474 399 282 85 75 25 20 BspEI T'CCGG_A Cuts at: 5712 5712 Size: 6049 BspHI T'CATG_A Cuts at: 3938 5668 3938 Size: 1730 4319 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3331 3404 844 Size: 2487 73 3489 Fragments arranged by size: 3489 2487 73 BsrBI CCG'CTC Cuts at: 1394 1823 2081 3262 3936 1394 Size: 429 258 1181 674 3507 Fragments arranged by size: 3507 1181 674 429 258 BsrDI GCAATG_nn' Cuts at: 65 1248 2571 2583 4544 4718 65 Size: 1183 1323 12 1961 174 1396 Fragments arranged by size: 1961 1396 1323 1183 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 4693 4880 1700 Size: 110 2883 187 2869 Fragments arranged by size: 2883 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6049 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6049 BssSI C'ACGA_G Cuts at: 4109 5121 4109 Size: 1012 5037 BstAPI GCAn_nnn'nTGC Cuts at: 794 2406 794 Size: 1612 4437 BstBI TT'CG_AA Cuts at: 4853 4853 Size: 6049 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6049 BstYI r'GATC_y Cuts at: 1972 2988 3379 3746 4131 4148 5600 5686 5698 Size: 1016 391 367 385 17 1452 86 12 Cuts at: 5698 6044 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1016 391 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3039 513 Size: 552 939 1035 3523 Fragments arranged by size: 3523 1035 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2587 487 Size: 683 56 1361 3949 Fragments arranged by size: 3949 1361 683 56 BtsI GCAGTG_nn' Cuts at: 2559 3565 4359 4379 2559 Size: 1006 794 20 4229 Fragments arranged by size: 4229 1006 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6049 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2995 3030 529 Size: 35 2431 35 3548 Fragments arranged by size: 3548 2431 35 35 DraI TTT'AAA Cuts at: 3353 3353 Size: 6049 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5056 809 Size: 507 3740 1802 Fragments arranged by size: 3740 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2556 2598 2928 Size: 54 1425 50 275 18 726 42 330 Cuts at: 2928 3496 4385 6014 8 Size: 568 889 1629 43 Fragments arranged by size: 1629 1425 889 726 568 330 275 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 3496 1537 Size: 275 1684 4090 Fragments arranged by size: 4090 1684 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2678 3149 3979 1353 Size: 1325 471 830 3423 Fragments arranged by size: 3423 1325 830 471 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2246 4636 5010 5156 387 Size: 1064 138 657 2390 374 146 1280 Fragments arranged by size: 2390 1280 1064 657 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2393 2445 2657 2772 3173 3320 4112 Size: 393 761 52 212 115 401 147 792 Cuts at: 4112 4709 5496 5764 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 792 787 761 597 401 393 268 212 147 115 52 EcoO109I rG'GnC_Cy Cuts at: 2728 2728 Size: 6049 EcoRI G'AATT_C Cuts at: 3358 3736 3358 Size: 378 5671 FspI TGC'GCA Cuts at: 4555 4555 Size: 6049 HaeII r_GCGC'y Cuts at: 1678 3130 5196 1678 Size: 1452 2066 2531 Fragments arranged by size: 2531 2066 1452 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2770 2802 2807 2839 4690 Size: 32 135 32 1198 32 5 32 1851 Cuts at: 4690 4722 4764 4796 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1851 1198 135 42 32 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 669 1657 1938 2857 3393 3639 669 Size: 988 281 919 536 246 3079 Fragments arranged by size: 3079 988 919 536 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6049 HpaI GTT'AAC Cuts at: 3639 3639 Size: 6049 KasI G'GCGC_C Cuts at: 3126 3126 Size: 6049 KpnI G_GTAC'C Cuts at: 3374 3374 Size: 6049 MfeI C'AATT_G Cuts at: 3648 3648 Size: 6049 MluI A'CGCG_T Cuts at: 5925 5925 Size: 6049 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5163 5347 1305 Size: 3858 184 2007 Fragments arranged by size: 3858 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2558 2600 2930 10 Size: 54 1425 343 726 42 330 3129 Fragments arranged by size: 3129 1425 726 343 330 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 2576 2621 4007 4366 4525 518 Size: 648 1410 45 1386 359 159 2042 Fragments arranged by size: 2042 1410 1386 648 359 159 45 MspA1I CmG'CkG Cuts at: 1606 2839 3465 4142 5290 5535 1606 Size: 1233 626 677 1148 245 2120 Fragments arranged by size: 2120 1233 1148 677 626 245 NarI GG'CG_CC Cuts at: 3127 3127 Size: 6049 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4558 Fragments arranged by size: 4558 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6049 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6049 NotI GC'GGCC_GC Cuts at: 3496 3496 Size: 6049 NspI r_CATG'y Cuts at: 1132 1229 2371 3190 3341 3407 4952 1132 Size: 97 1142 819 151 66 1545 2229 Fragments arranged by size: 2229 1545 1142 819 151 97 66 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6049 PciI A'CATG_T Cuts at: 4948 4948 Size: 6049 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6049 PmeI GTTT'AAAC Cuts at: 3353 3353 Size: 6049 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2804 2836 4118 4150 5631 5663 2804 Size: 32 1282 32 1481 32 3190 Fragments arranged by size: 3190 1481 1282 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6049 PsiI TTA'TAA Cuts at: 3619 3619 Size: 6049 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2096 2128 2096 Size: 32 6017 PstI C_TGCA'G Cuts at: 830 3401 830 Size: 2571 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6049 PvuII CAG'CTG Cuts at: 2839 2839 Size: 6049 SalI G'TCGA_C Cuts at: 1936 2855 3391 1936 Size: 919 536 4594 Fragments arranged by size: 4594 919 536 SapI GCTCTTCn'nnn_ Cuts at: 2678 2678 Size: 6049 SbfI CC_TGCA'GG Cuts at: 3401 3401 Size: 6049 ScaI AGT'ACT Cuts at: 2111 4297 6009 2111 Size: 2186 1712 2151 Fragments arranged by size: 2186 2151 1712 SfcI C'TryA_G Cuts at: 826 1045 3220 3397 4532 5213 5404 826 Size: 219 2175 177 1135 681 191 1471 Fragments arranged by size: 2175 1471 1135 681 219 191 177 SfoI GGC'GCC Cuts at: 3128 3128 Size: 6049 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6049 SmaI CCC'GGG Cuts at: 1872 2679 3376 1872 Size: 807 697 4545 Fragments arranged by size: 4545 807 697 SmlI C'TyrA_G Cuts at: 820 1017 1943 3062 4153 5054 5316 820 Size: 197 926 1119 1091 901 262 1553 Fragments arranged by size: 1553 1119 1091 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3840 493 Size: 3347 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6049 SphI G_CATG'C Cuts at: 1132 3190 3407 1132 Size: 2058 217 3774 Fragments arranged by size: 3774 2058 217 SspI AAT'ATT Cuts at: 5 52 3973 5 Size: 47 3921 2081 Fragments arranged by size: 3921 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6049 StyI C'CwwG_G Cuts at: 513 1065 2004 2439 2545 3488 513 Size: 552 939 435 106 943 3074 Fragments arranged by size: 3074 943 939 552 435 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2398 4076 4093 4246 4431 4876 325 Size: 1255 818 1678 17 153 185 445 1498 Fragments arranged by size: 1678 1498 1255 818 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2109 4295 6007 Size: 274 80 33 51 1443 132 2186 1712 Cuts at: 6007 96 Size: 138 Fragments arranged by size: 2186 1712 1443 274 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2494 2526 3269 3301 6009 6041 2494 Size: 32 743 32 2708 32 2502 Fragments arranged by size: 2708 2502 743 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6049 XbaI T'CTAG_A Cuts at: 3385 3385 Size: 6049 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2446 2593 2011 Size: 435 147 5467 Fragments arranged by size: 5467 435 147 XhoI C'TCGA_G Cuts at: 1943 3062 1943 Size: 1119 4930 XmaI C'CCGG_G Cuts at: 1870 2677 3374 1870 Size: 807 697 4545 Fragments arranged by size: 4545 807 697 XmnI GAAnn'nnTTC Cuts at: 4178 4178 Size: 6049 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3857 276 Size: 53 83 186 936 2323 2468 Fragments arranged by size: 2468 2323 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI