Restriction map for
pFN21AB0419
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspLU11I
BspMI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraIII DrdI DsaI Eam1105I
Eco47III Eco81I EcoNI EcoRV EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tsp45I Tth111I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AclI 268 AACGTT AA^CGTT
AlwI 586 GATCC GGATC(4/5)
AlwNI 1240 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1227 GGGCCC GGGCC^C
Asp700I 376 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 852 GAGCAC G[AT]GC[AT]^C
BciVI 1294 GGATAC GTATCC(6/5)
BclI 816 TGATCA T^GATCA
BcnI 45 CCGGG CC^[GC]GG
BfaI 1382 CTAG C^TAG
BsaI 785 GAGACC GGTCTC(1/5)
BsiEI 68 CGGCCG CG[GA][CT]^CG
BsiHKAI 852 GAGCAC G[AT]GC[AT]^C
BsmAI 785 GAGAC GTCTC(1/5)
BsmFI 234 GGGAC GGGAC(10/14)
Bsp1407I 870 TGTACA T^GTACA
BspHI 1011 TCATGA T^CATGA
BsrBI 931 CCGCTC CCGCTC(-3/-3)
BsrDI 425 CATTGC GCAATG(2/0)
BsrFI 429 GCCGGT [GA]^CCGG[CT]
BsrGI 870 TGTACA T^GTACA
BstEII 1300 GGTAACC G^GT[ACGT]ACC
BstPI 1300 GGTAACC G^GT[ACGT]ACC
Cfr10I 429 GCCGGT [GA]^CCGG[CT]
EagI 68 CGGCCG C^GGCCG
EclXI 68 CGGCCG C^GGCCG
Eco52I 68 CGGCCG C^GGCCG
EcoO65I 1300 GGTAACC G^GT[ACGT]ACC
EcoT14I 1305 CCAAGG C^C[AT][AT]GG
FauI 879 GCGGG CCCGC(4/6)
FbaI 816 TGATCA T^GATCA
HindIII 598 AAGCTT A^AGCTT
MaeI 1382 CTAG C^TAG
MaeII 269 ACGT A^CGT
MseI 551 TTAA T^TAA
NciI 45 CCGGG CC^[GC]GG
PflMI 1182 CCAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 621 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 268 AACGTT AA^CGTT
PvuII 1243 CAGCTG CAG^CTG
RcaI 1011 TCATGA T^CATGA
SmlI 119 CTCAAG C^T[CT][GA]AG
SspBI 870 TGTACA T^GTACA
StuI 461 AGGCCT AGG^CCT
StyI 1305 CCAAGG C^C[AT][AT]GG
TaqI 998 TCGA T^CGA
Tru9I 551 TTAA T^TAA
TthHB8I 998 TCGA T^CGA
Van91I 1182 CCAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1239 CCAGCAGCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 376 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BalI 605 TGGCCA TGG^CCA
1275 TGGCCA
BanII 116 GGGCTC G[GA]GC[CT]^C
1227 GGGCCC
BseRI 447 GAGGAG GAGGAG(10/8)
671 GAGGAG
BsgI 79 GTGCAG GTGCAG(16/14)
469 GTGCAG
DpnII 586 GATC ^GATC
817 GATC
DraII 621 AGGTCCT [GA]G^G[ACGT]CC[CT]
1226 GGGGCCC
EcoO109I 621 AGGTCCT [GA]G^G[ACGT]CC[CT]
1226 GGGGCCC
EcoRI 1083 GAATTC G^AATTC
1143 GAATTC
HapII 45 CCGG C^CGG
430 CCGG
HinfI 29 GATTC G^A[ACGT]TC
184 GAATC
HpaII 45 CCGG C^CGG
430 CCGG
MboI 586 GATC ^GATC
817 GATC
MluNI 605 TGGCCA TGG^CCA
1275 TGGCCA
MscI 605 TGGCCA TGG^CCA
1275 TGGCCA
MspA1I 613 CAGCGG C[AC]G^C[GT]G
1243 CAGCTG
MspI 45 CCGG C^CGG
430 CCGG
NdeII 586 GATC ^GATC
817 GATC
NlaIV 1226 GGGGCC GG[ACGT]^[ACGT]CC
1227 GGGCCC
NspI 875 ACATGC [GA]CATG^[CT]
1385 GCATGT
Sau3AI 586 GATC ^GATC
817 GATC
SfaNI 1018 GATGC GCATC(5/9)
1348 GCATC
TfiI 29 GATTC G^A[AT]TC
184 GAATC
TspRI 865 CACTG CA[GC]TG[ACGT][ACGT]^
1313 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EaeI
EarI EcoRII Fnu4HI FokI HaeIII
HphI ItaI Ksp632I MaeIII MboII
MnlI MslI MvaI MwoI NlaIII
RsaI Sau96I ScrFI SfcI TseI
Tsp509I