(Circular) (Six-base) MAPSORT of: pFN21AB0419.seq Check: 1178 from: 1 to: 6121 pFN21AB0419 6121 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3931 278 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3464 1937 Size: 1527 4594 Acc65I G'GTAC_C Cuts at: 3442 3442 Size: 6121 AclI AA'CG_TT Cuts at: 1563 2274 4248 4621 1563 Size: 711 1974 373 3063 Fragments arranged by size: 3063 1974 711 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2592 4184 5568 5836 1632 Size: 960 1592 1384 268 1917 Fragments arranged by size: 1917 1592 1384 960 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5924 AflIII A'CryG_T Cuts at: 1203 5020 5997 1203 Size: 3817 977 1327 Fragments arranged by size: 3817 1327 977 AgeI A'CCGG_T Cuts at: 4952 4952 Size: 6121 AhdI GACnn_n'nnGTC Cuts at: 4850 4850 Size: 6121 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6121 AlwNI CAG_nnn'CTG Cuts at: 3250 3864 5436 3250 Size: 614 1572 3935 Fragments arranged by size: 3935 1572 614 ApaI G_GGCC'C Cuts at: 3236 3236 Size: 6121 ApaLI G'TGCA_C Cuts at: 4178 5334 4178 Size: 1156 4965 ApoI r'AATT_y Cuts at: 1493 2109 3088 3148 3430 3656 3808 5917 1493 Size: 616 979 60 282 226 152 2109 1697 Fragments arranged by size: 2109 1697 979 616 282 226 152 60 AseI AT'TA_AT Cuts at: 160 1032 4675 160 Size: 872 3643 1606 Fragments arranged by size: 3643 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 AvaI C'yCGr_G Cuts at: 1870 1943 3446 1870 Size: 73 1503 4545 Fragments arranged by size: 4545 1503 73 BamHI G'GATC_C Cuts at: 3451 3818 3451 Size: 367 5754 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3442 4897 618 Size: 325 217 627 1655 1455 1842 Fragments arranged by size: 1842 1655 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2125 3236 3440 1391 Size: 734 1111 204 4072 Fragments arranged by size: 4072 1111 734 204 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5150 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4312 4346 1903 Size: 34 2375 34 3678 Fragments arranged by size: 3678 2375 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3293 4013 5223 3293 Size: 720 1210 4191 Fragments arranged by size: 4191 1210 720 BclI T'GATC_A Cuts at: 1548 2821 1548 Size: 1273 4848 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4732 136 Size: 107 122 71 631 605 3060 1525 Fragments arranged by size: 3060 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6116 6116 Size: 6121 BlpI GC'TnA_GC Cuts at: 3538 3538 Size: 6121 Bme1580I G_kGCm'C Cuts at: 1790 2018 2538 3236 4182 5338 1790 Size: 228 520 698 946 1156 2573 Fragments arranged by size: 2573 1156 946 698 520 228 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6121 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3852 4810 442 Size: 416 53 83 399 24 2435 958 1753 Fragments arranged by size: 2435 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4781 1239 Size: 3542 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2109 4246 5111 5409 2109 Size: 2137 865 298 2821 Fragments arranged by size: 2821 2137 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2784 4784 882 Size: 617 1285 2000 2219 Fragments arranged by size: 2219 2000 1285 617 BsaAI yAC'GTr Cuts at: 493 1204 3912 493 Size: 711 2708 2702 Fragments arranged by size: 2708 2702 711 BsaBI GATnn'nnATC Cuts at: 6115 6115 Size: 6121 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3928 4310 4945 275 Size: 53 83 186 936 2395 382 635 1451 Fragments arranged by size: 2395 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4554 4952 5226 5373 5784 4554 Size: 398 274 147 411 4891 Fragments arranged by size: 4891 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5896 Fragments arranged by size: 5896 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2467 2691 1186 Size: 1281 224 4616 Fragments arranged by size: 4616 1281 224 BseYI C'CCAG_C Cuts at: 3235 5324 5820 3235 Size: 2089 496 3536 Fragments arranged by size: 3536 2089 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2105 2495 1163 Size: 573 369 390 4789 Fragments arranged by size: 4789 573 390 369 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2076 3571 4332 5360 1540 Size: 275 186 75 1495 761 1028 2301 Fragments arranged by size: 2301 1495 1028 761 275 186 75 BsiHKAI G_wGCw'C Cuts at: 2861 4182 4267 5338 2861 Size: 1321 85 1071 3644 Fragments arranged by size: 3644 1321 1071 85 BsmI GAATG_Cn' Cuts at: 3632 3725 3632 Size: 93 6028 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2125 2538 2861 3236 3440 4182 Size: 399 228 107 413 323 375 204 742 Cuts at: 4182 4267 5338 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 413 399 375 323 228 204 107 85 BspEI T'CCGG_A Cuts at: 5784 5784 Size: 6121 BspHI T'CATG_A Cuts at: 3016 4010 5740 3016 Size: 994 1730 3397 Fragments arranged by size: 3397 1730 994 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3476 844 Size: 2632 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2938 4008 1394 Size: 429 1115 1070 3507 Fragments arranged by size: 3507 1115 1070 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2429 4616 4790 65 Size: 1183 1181 2187 174 1396 Fragments arranged by size: 2187 1396 1183 1181 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2434 4765 4952 1700 Size: 110 624 2331 187 2869 Fragments arranged by size: 2869 2331 624 187 110 BsrGI T'GTAC_A Cuts at: 96 2875 96 Size: 2779 3342 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6121 BssSI C'ACGA_G Cuts at: 4181 5193 4181 Size: 1012 5109 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6121 BstBI TT'CG_AA Cuts at: 4925 4925 Size: 6121 BstEII G'GTnAC_C Cuts at: 1184 3305 1184 Size: 2121 4000 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6121 BstYI r'GATC_y Cuts at: 1972 3451 3818 4203 4220 5672 5758 5770 6116 Size: 1479 367 385 17 1452 86 12 346 Cuts at: 6116 1972 Size: 1977 Fragments arranged by size: 1977 1479 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5569 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5382 Fragments arranged by size: 5382 683 56 BtsI GCAGTG_nn' Cuts at: 3324 3637 4431 4451 3324 Size: 313 794 20 4994 Fragments arranged by size: 4994 794 313 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6121 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6086 DraI TTT'AAA Cuts at: 3425 3425 Size: 6121 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5128 809 Size: 507 3812 1802 Fragments arranged by size: 3812 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2325 2610 Size: 54 1425 50 275 18 243 252 285 Cuts at: 2610 2621 3280 3568 4457 6086 8 Size: 11 659 288 889 1629 43 Fragments arranged by size: 1629 1425 889 659 288 285 275 252 243 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 2073 3568 1537 Size: 275 261 1495 4090 Fragments arranged by size: 4090 1495 275 261 EarI CTCTTCn'nnn_ Cuts at: 1353 2782 3086 3146 4051 1353 Size: 1429 304 60 905 3423 Fragments arranged by size: 3423 1429 905 304 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4708 5082 5228 387 Size: 1064 138 3119 374 146 1280 Fragments arranged by size: 3119 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2592 4184 4781 5568 5836 1239 Size: 393 960 1592 597 787 268 1524 Fragments arranged by size: 1592 1524 960 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2627 3232 2627 Size: 605 5516 EcoRI G'AATT_C Cuts at: 3088 3148 3430 3808 3088 Size: 60 282 378 5401 Fragments arranged by size: 5401 378 282 60 FspI TGC'GCA Cuts at: 4627 4627 Size: 6121 HaeII r_GCGC'y Cuts at: 1678 5268 1678 Size: 3590 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2828 2860 4762 4794 4836 Size: 32 135 32 1256 32 1902 32 42 Cuts at: 4836 4868 1373 Size: 32 2626 Fragments arranged by size: 2626 1902 1256 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3465 3711 669 Size: 988 281 1527 246 3079 Fragments arranged by size: 3079 1527 988 281 246 HindIII A'AGCT_T Cuts at: 748 2603 748 Size: 1855 4266 HpaI GTT'AAC Cuts at: 3711 3711 Size: 6121 KpnI G_GTAC'C Cuts at: 3446 3446 Size: 6121 MfeI C'AATT_G Cuts at: 3720 3720 Size: 6121 MluI A'CGCG_T Cuts at: 5997 5997 Size: 6121 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2385 5235 5419 1305 Size: 1080 2850 184 2007 Fragments arranged by size: 2850 2007 1080 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2612 3282 10 Size: 54 1425 343 780 670 2849 Fragments arranged by size: 2849 1425 780 670 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2247 3021 3069 3129 4079 4438 4597 Size: 648 1081 774 48 60 950 359 159 Cuts at: 4597 518 Size: 2042 Fragments arranged by size: 2042 1081 950 774 648 359 159 60 48 MspA1I CmG'CkG Cuts at: 1606 2620 3250 3537 4214 5362 5607 1606 Size: 1014 630 287 677 1148 245 2120 Fragments arranged by size: 2120 1148 1014 677 630 287 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5569 NdeI CA'TA_TG Cuts at: 387 387 Size: 6121 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6121 NotI GC'GGCC_GC Cuts at: 3568 3568 Size: 6121 NspI r_CATG'y Cuts at: 1132 1229 2884 3394 3479 5024 1132 Size: 97 1655 510 85 1545 2229 Fragments arranged by size: 2229 1655 1545 510 97 85 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6121 PciI A'CATG_T Cuts at: 5020 5020 Size: 6121 PflMI CCAn_nnn'nTGG Cuts at: 1108 3193 1108 Size: 2085 4036 PmeI GTTT'AAAC Cuts at: 3425 3425 Size: 6121 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4190 4222 5703 5735 4190 Size: 32 1481 32 4576 Fragments arranged by size: 4576 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2627 2627 Size: 6121 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6121 PsiI TTA'TAA Cuts at: 2922 3691 2922 Size: 769 5352 PspOMI G'GGCC_C Cuts at: 3232 3232 Size: 6121 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3314 3346 3314 Size: 32 6089 PstI C_TGCA'G Cuts at: 830 3473 830 Size: 2643 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6121 PvuII CAG'CTG Cuts at: 3250 3250 Size: 6121 SalI G'TCGA_C Cuts at: 1936 3463 1936 Size: 1527 4594 SbfI CC_TGCA'GG Cuts at: 3473 3473 Size: 6121 ScaI AGT'ACT Cuts at: 4369 6081 4369 Size: 1712 4409 SfcI C'TryA_G Cuts at: 826 1045 3093 3153 3334 3469 4604 5285 5476 Size: 219 2048 60 181 135 1135 681 191 Cuts at: 5476 826 Size: 1471 Fragments arranged by size: 2048 1471 1135 681 219 191 181 135 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6121 SmaI CCC'GGG Cuts at: 1872 3448 1872 Size: 1576 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2124 4225 5126 5388 820 Size: 197 926 181 2101 901 262 1553 Fragments arranged by size: 2101 1553 926 901 262 197 181 SnaBI TAC'GTA Cuts at: 493 3912 493 Size: 3419 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6121 SphI G_CATG'C Cuts at: 1132 3479 1132 Size: 2347 3774 SspI AAT'ATT Cuts at: 5 52 4045 5 Size: 47 3993 2081 Fragments arranged by size: 3993 2081 47 StuI AGG'CCT Cuts at: 1517 2468 1517 Size: 951 5170 StyI C'CwwG_G Cuts at: 513 1065 3310 3560 513 Size: 552 2245 250 3074 Fragments arranged by size: 3074 2245 552 250 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2278 4148 4165 4318 4503 4948 325 Size: 1255 698 1870 17 153 185 445 1498 Fragments arranged by size: 1870 1498 1255 698 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2875 2953 4367 Size: 274 80 33 51 1443 898 78 1414 Cuts at: 4367 6079 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1414 898 274 138 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2025 2057 2686 2718 6081 6113 2025 Size: 32 629 32 3363 32 2033 Fragments arranged by size: 3363 2033 629 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6121 XbaI T'CTAG_A Cuts at: 3457 3457 Size: 6121 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3251 3251 Size: 6121 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6121 XmaI C'CCGG_G Cuts at: 1870 3446 1870 Size: 1576 4545 XmnI GAAnn'nnTTC Cuts at: 2385 4250 2385 Size: 1865 4256 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3929 276 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI