Restriction map for
pFN21AB0419
(Linear) (Six-base) MAPSORT of: KIBB0419.seq Check: 1789 from: 1 to: 1416
KIBB0419.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AclI AA'CG_TT
Cuts at: 0 269 1416
Size: 269 1147
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 1416
Size: 587 829
AlwNI CAG_nnn'CTG
Cuts at: 0 1245 1416
Size: 1245 171
ApaI G_GGCC'C
Cuts at: 0 1231 1416
Size: 1231 185
ApoI r'AATT_y
Cuts at: 0 104 1083 1143 1416
Size: 104 979 60 273
Fragments arranged by size:
979 273 104 60
BanII G_rGCy'C
Cuts at: 0 120 1231 1416
Size: 120 1111 185
Fragments arranged by size:
1111 185 120
BciVI GTATCCnnnnn_n'
Cuts at: 0 1288 1416
Size: 1288 128
BclI T'GATC_A
Cuts at: 0 816 1416
Size: 816 600
Bme1580I G_kGCm'C
Cuts at: 0 13 533 1231 1416
Size: 13 520 698 185
Fragments arranged by size:
698 520 185 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 1416
Size: 104 1312
BsaI GGTCTCn'nnnn_
Cuts at: 0 779 1416
Size: 779 637
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 462 686 1416
Size: 462 224 730
Fragments arranged by size:
730 462 224
BseYI C'CCAG_C
Cuts at: 0 1230 1416
Size: 1230 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 490 1416
Size: 100 390 926
Fragments arranged by size:
926 390 100
BsiEI CG_ry'CG
Cuts at: 0 71 1416
Size: 71 1345
BsiHKAI G_wGCw'C
Cuts at: 0 856 1416
Size: 856 560
Bsp1286I G_dGCh'C
Cuts at: 0 13 120 533 856 1231 1416
Size: 13 107 413 323 375 185
Fragments arranged by size:
413 375 323 185 107 13
BspHI T'CATG_A
Cuts at: 0 1011 1416
Size: 1011 405
BsrBI CCG'CTC
Cuts at: 0 933 1416
Size: 933 483
BsrDI GCAATG_nn'
Cuts at: 0 424 1416
Size: 424 992
BsrFI r'CCGG_y
Cuts at: 0 429 1416
Size: 429 987
BsrGI T'GTAC_A
Cuts at: 0 870 1416
Size: 870 546
BstEII G'GTnAC_C
Cuts at: 0 1300 1416
Size: 1300 116
BtsI GCAGTG_nn'
Cuts at: 0 1319 1416
Size: 1319 97
EaeI y'GGCC_r
Cuts at: 0 68 320 605 616 1275 1416
Size: 68 252 285 11 659 141
Fragments arranged by size:
659 285 252 141 68 11
EagI C'GGCC_G
Cuts at: 0 68 1416
Size: 68 1348
EarI CTCTTCn'nnn_
Cuts at: 0 777 1081 1141 1416
Size: 777 304 60 275
Fragments arranged by size:
777 304 275 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 1416
Size: 587 829
EcoO109I rG'GnC_Cy
Cuts at: 0 622 1227 1416
Size: 622 605 189
Fragments arranged by size:
622 605 189
EcoRI G'AATT_C
Cuts at: 0 1083 1143 1416
Size: 1083 60 273
Fragments arranged by size:
1083 273 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 823 855 1416
Size: 823 32 561
Fragments arranged by size:
823 561 32
HindIII A'AGCT_T
Cuts at: 0 598 1416
Size: 598 818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1416
Size: 380 1036
MscI TGG'CCA
Cuts at: 0 607 1277 1416
Size: 607 670 139
Fragments arranged by size:
670 607 139
MslI CAynn'nnrTG
Cuts at: 0 242 1016 1064 1124 1416
Size: 242 774 48 60 292
Fragments arranged by size:
774 292 242 60 48
MspA1I CmG'CkG
Cuts at: 0 615 1245 1416
Size: 615 630 171
Fragments arranged by size:
630 615 171
NspI r_CATG'y
Cuts at: 0 879 1389 1416
Size: 879 510 27
Fragments arranged by size:
879 510 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 1188 1416
Size: 1188 228
PpuMI rG'GwC_Cy
Cuts at: 0 622 1416
Size: 622 794
PsiI TTA'TAA
Cuts at: 0 917 1416
Size: 917 499
PspOMI G'GGCC_C
Cuts at: 0 1227 1416
Size: 1227 189
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1309 1341 1416
Size: 1309 32 75
Fragments arranged by size:
1309 75 32
PvuII CAG'CTG
Cuts at: 0 1245 1416
Size: 1245 171
SfcI C'TryA_G
Cuts at: 0 1088 1148 1329 1416
Size: 1088 60 181 87
Fragments arranged by size:
1088 181 87 60
SmlI C'TyrA_G
Cuts at: 0 119 1416
Size: 119 1297
StuI AGG'CCT
Cuts at: 0 463 1416
Size: 463 953
StyI C'CwwG_G
Cuts at: 0 1305 1416
Size: 1305 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 273 1416
Size: 273 1143
TatI w'GTAC_w
Cuts at: 0 870 948 1416
Size: 870 78 468
Fragments arranged by size:
870 468 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 20 52 681 713 1416
Size: 20 32 629 32 703
Fragments arranged by size:
703 629 32 32 20
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1246 1416
Size: 1246 170
XmnI GAAnn'nnTTC
Cuts at: 0 380 1416
Size: 380 1036
Enzymes that do cut:
AclI AcuI AlwNI ApaI ApoI BanII BciVI BclI
Bme1580I BpuEI BsaI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI BstEII BtsI
EaeI EagI EarI Eco57MI EcoO109I EcoRI Hin4I HindIII
MmeI MscI MslI MspA1I NspI PflMI PpuMI PsiI
PspOMI PsrI PvuII SfcI SmlI StuI StyI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI
BsiWI BsmI BsmBI BspEI BspMI BssHII BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EciI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PspXI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmaI ZraI