(Circular) (Six-base) MAPSORT of: pFN21AB0415.seq Check: 3499 from: 1 to: 6109 pFN21AB0415 6109 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3919 278 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3452 1937 Size: 1515 4594 Acc65I G'GTAC_C Cuts at: 3430 3430 Size: 6109 AclI AA'CG_TT Cuts at: 1563 4236 4609 1563 Size: 2673 373 3063 Fragments arranged by size: 3063 2673 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3022 3069 3215 4172 5556 5824 1632 Size: 1390 47 146 957 1384 268 1917 Fragments arranged by size: 1917 1390 1384 957 268 146 47 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5912 AflIII A'CryG_T Cuts at: 1203 5008 5985 1203 Size: 3805 977 1327 Fragments arranged by size: 3805 1327 977 AgeI A'CCGG_T Cuts at: 3295 4940 3295 Size: 1645 4464 AhdI GACnn_n'nnGTC Cuts at: 4838 4838 Size: 6109 AleI CACnn'nnGTG Cuts at: 1166 2681 3269 1166 Size: 1515 588 4006 Fragments arranged by size: 4006 1515 588 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2898 1184 Size: 1714 4395 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2969 3001 2969 Size: 32 6077 AlwNI CAG_nnn'CTG Cuts at: 2678 3852 5424 2678 Size: 1174 1572 3363 Fragments arranged by size: 3363 1572 1174 ApaLI G'TGCA_C Cuts at: 2321 4166 5322 2321 Size: 1845 1156 3108 Fragments arranged by size: 3108 1845 1156 ApoI r'AATT_y Cuts at: 1493 3418 3644 3796 5905 1493 Size: 1925 226 152 2109 1697 Fragments arranged by size: 2109 1925 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4663 160 Size: 872 3631 1606 Fragments arranged by size: 3631 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 AvaI C'yCGr_G Cuts at: 1870 1943 2146 2345 3331 3434 1870 Size: 73 203 199 986 103 4545 Fragments arranged by size: 4545 986 203 199 103 73 BamHI G'GATC_C Cuts at: 3439 3806 3439 Size: 367 5742 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3430 4885 618 Size: 325 217 627 1643 1455 1842 Fragments arranged by size: 1842 1643 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2355 2381 2769 2927 3193 3371 3428 1391 Size: 964 26 388 158 266 178 57 4072 Fragments arranged by size: 4072 964 388 266 178 158 57 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2169 2487 3179 928 Size: 971 270 318 692 3858 Fragments arranged by size: 3858 971 692 318 270 BbvCI CC'TCA_GC Cuts at: 2252 2384 2252 Size: 132 5977 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2086 2120 4300 4334 1903 Size: 34 50 34 65 34 2180 34 3678 Fragments arranged by size: 3678 2180 65 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4001 5211 4001 Size: 1210 4899 BclI T'GATC_A Cuts at: 1548 1548 Size: 6109 BfrBI ATG'CAT Cuts at: 3217 3217 Size: 6109 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2042 4720 Size: 107 122 71 631 605 337 33 2678 Cuts at: 4720 136 Size: 1525 Fragments arranged by size: 2678 1525 631 605 337 122 107 71 33 BglII A'GATC_T Cuts at: 6104 6104 Size: 6109 BlpI GC'TnA_GC Cuts at: 3526 3526 Size: 6109 Bme1580I G_kGCm'C Cuts at: 1790 2325 2887 2942 4170 5326 1790 Size: 535 562 55 1228 1156 2573 Fragments arranged by size: 2573 1228 1156 562 535 55 BmgBI CAC'GTC Cuts at: 1318 3177 1318 Size: 1859 4250 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2191 3062 3263 Size: 416 53 83 399 24 774 871 201 Cuts at: 3263 3840 4798 442 Size: 577 958 1753 Fragments arranged by size: 1753 958 871 774 577 416 399 201 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6109 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2807 2872 2952 3135 4769 1239 Size: 1568 65 80 183 1634 2579 Fragments arranged by size: 2579 1634 1568 183 80 65 Bpu10I CC'TnA_GC Cuts at: 2189 2252 2384 2189 Size: 63 132 5914 Fragments arranged by size: 5914 132 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4234 5099 5397 4234 Size: 865 298 4946 Fragments arranged by size: 4946 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4772 882 Size: 617 3273 2219 Fragments arranged by size: 3273 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2869 3900 493 Size: 711 1665 1031 2702 Fragments arranged by size: 2702 1665 1031 711 BsaBI GATnn'nnATC Cuts at: 6103 6103 Size: 6109 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2847 3916 4298 4933 Size: 53 83 186 936 1314 1069 382 635 Cuts at: 4933 275 Size: 1451 Fragments arranged by size: 1451 1314 1069 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2370 3295 4542 4940 5214 5361 5772 2370 Size: 925 1247 398 274 147 411 2707 Fragments arranged by size: 2707 1247 925 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2115 2145 2714 2744 1178 Size: 30 165 30 712 30 569 30 4543 Fragments arranged by size: 4543 712 569 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2363 2906 3131 1186 Size: 1177 543 225 4164 Fragments arranged by size: 4164 1177 543 225 BseYI C'CCAG_C Cuts at: 2216 2308 2312 2355 3305 5312 5808 2216 Size: 92 4 43 950 2007 496 2517 Fragments arranged by size: 2517 2007 950 496 92 43 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2282 2894 1163 Size: 573 546 612 4378 Fragments arranged by size: 4378 612 573 546 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2305 2478 3559 4320 5348 1540 Size: 275 186 304 173 1081 761 1028 2301 Fragments arranged by size: 2301 1081 1028 761 304 275 186 173 BsiHKAI G_wGCw'C Cuts at: 2190 2325 2420 2448 2769 4170 4255 5326 2190 Size: 135 95 28 321 1401 85 1071 2973 Fragments arranged by size: 2973 1401 1071 321 135 95 85 28 BsmI GAATG_Cn' Cuts at: 3620 3713 3620 Size: 93 6016 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2190 2325 2355 2381 2420 2448 2769 Size: 399 400 135 30 26 39 28 321 Cuts at: 2769 2887 2927 2942 3193 3371 3428 4170 4255 Size: 118 40 15 251 178 57 742 85 Cuts at: 4255 5326 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 400 399 321 251 178 135 118 85 57 40 39 30 28 26 15 BspEI T'CCGG_A Cuts at: 2370 5772 2370 Size: 3402 2707 BspHI T'CATG_A Cuts at: 3998 5728 3998 Size: 1730 4379 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2150 3464 844 Size: 1306 1314 3489 Fragments arranged by size: 3489 1314 1306 BsrBI CCG'CTC Cuts at: 1394 1823 2057 2856 3996 1394 Size: 429 234 799 1140 3507 Fragments arranged by size: 3507 1140 799 429 234 BsrDI GCAATG_nn' Cuts at: 65 1248 2395 3147 4604 4778 65 Size: 1183 1147 752 1457 174 1396 Fragments arranged by size: 1457 1396 1183 1147 752 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2197 2300 3295 4753 4940 1700 Size: 110 387 103 995 1458 187 2869 Fragments arranged by size: 2869 1458 995 387 187 110 103 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6109 BssHII G'CGCG_C Cuts at: 1925 2022 1925 Size: 97 6012 BssSI C'ACGA_G Cuts at: 4169 5181 4169 Size: 1012 5097 BstAPI GCAn_nnn'nTGC Cuts at: 794 2872 794 Size: 2078 4031 BstBI TT'CG_AA Cuts at: 4913 4913 Size: 6109 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6109 BstXI CCAn_nnnn'nTGG Cuts at: 2276 3106 3313 2276 Size: 830 207 5072 Fragments arranged by size: 5072 830 207 BstYI r'GATC_y Cuts at: 1972 3439 3806 4191 4208 5660 5746 5758 6104 Size: 1467 367 385 17 1452 86 12 346 Cuts at: 6104 1972 Size: 1977 Fragments arranged by size: 1977 1467 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2861 2861 Size: 6109 BtgI C'CryG_G Cuts at: 513 1065 2682 2934 3362 513 Size: 552 1617 252 428 3260 Fragments arranged by size: 3260 1617 552 428 252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5370 Fragments arranged by size: 5370 683 56 BtsI GCAGTG_nn' Cuts at: 2676 3092 3625 4419 4439 2676 Size: 416 533 794 20 4346 Fragments arranged by size: 4346 794 533 416 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6109 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2891 2926 529 Size: 35 2327 35 3712 Fragments arranged by size: 3712 2327 35 35 DraI TTT'AAA Cuts at: 3413 3413 Size: 6109 DraIII CAC_nnn'GTG Cuts at: 2185 2872 2185 Size: 687 5422 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5116 809 Size: 507 3800 1802 Fragments arranged by size: 3800 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2043 2302 Size: 54 1425 50 275 18 180 33 259 Cuts at: 2302 2475 2685 3556 4445 6074 8 Size: 173 210 871 889 1629 43 Fragments arranged by size: 1629 1425 889 871 275 259 210 180 173 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2302 2475 3556 1537 Size: 275 490 173 1081 4090 Fragments arranged by size: 4090 1081 490 275 173 EarI CTCTTCn'nnn_ Cuts at: 1353 2369 3041 4039 1353 Size: 1016 672 998 3423 Fragments arranged by size: 3423 1016 998 672 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2646 3210 4696 5070 5216 387 Size: 1064 138 1057 564 1486 374 146 1280 Fragments arranged by size: 1486 1280 1064 1057 564 374 146 138 EcoICRI GAG'CTC Cuts at: 2767 2767 Size: 6109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2807 2872 2952 3022 3069 3135 3215 Size: 393 1175 65 80 70 47 66 80 Cuts at: 3215 4172 4769 5556 5824 1239 Size: 957 597 787 268 1524 Fragments arranged by size: 1524 1175 957 787 597 393 268 80 80 70 66 65 47 EcoO109I rG'GnC_Cy Cuts at: 2408 2818 3358 2408 Size: 410 540 5159 Fragments arranged by size: 5159 540 410 EcoRI G'AATT_C Cuts at: 3418 3796 3418 Size: 378 5731 EcoRV GAT'ATC Cuts at: 2774 2774 Size: 6109 FseI GG_CCGG'CC Cuts at: 2304 2304 Size: 6109 FspI TGC'GCA Cuts at: 2085 4615 2085 Size: 2530 3579 HaeII r_GCGC'y Cuts at: 1678 2063 2077 5256 1678 Size: 385 14 3179 2531 Fragments arranged by size: 3179 2531 385 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2714 2746 3314 3346 4750 Size: 32 135 32 1142 32 568 32 1404 Cuts at: 4750 4782 4824 4856 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1404 1142 568 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3453 3699 669 Size: 988 281 1515 246 3079 Fragments arranged by size: 3079 1515 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6109 HpaI GTT'AAC Cuts at: 3699 3699 Size: 6109 KpnI G_GTAC'C Cuts at: 3434 3434 Size: 6109 MfeI C'AATT_G Cuts at: 3708 3708 Size: 6109 MluI A'CGCG_T Cuts at: 5985 5985 Size: 6109 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5223 5407 1305 Size: 3918 184 2007 Fragments arranged by size: 3918 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2687 10 Size: 54 1425 343 855 3432 Fragments arranged by size: 3432 1425 855 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2681 2832 2993 3269 4067 4426 4585 Size: 648 1515 151 161 276 798 359 159 Cuts at: 4585 518 Size: 2042 Fragments arranged by size: 2042 1515 798 648 359 276 161 159 151 MspA1I CmG'CkG Cuts at: 1606 2312 2700 2972 3241 3525 4202 5350 5595 Size: 706 388 272 269 284 677 1148 245 Cuts at: 5595 1606 Size: 2120 Fragments arranged by size: 2120 1148 706 677 388 284 272 269 245 NaeI GCC'GGC Cuts at: 2199 2302 2199 Size: 103 6006 NcoI C'CATG_G Cuts at: 513 1065 2934 513 Size: 552 1869 3688 Fragments arranged by size: 3688 1869 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6109 NgoMIV G'CCGG_C Cuts at: 2197 2300 2197 Size: 103 6006 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6109 NotI GC'GGCC_GC Cuts at: 3556 3556 Size: 6109 NsiI A_TGCA'T Cuts at: 3219 3219 Size: 6109 NspI r_CATG'y Cuts at: 1132 1229 3217 3467 5012 1132 Size: 97 1988 250 1545 2229 Fragments arranged by size: 2229 1988 1545 250 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6109 PciI A'CATG_T Cuts at: 5008 5008 Size: 6109 PflMI CCAn_nnn'nTGG Cuts at: 1108 2223 1108 Size: 1115 4994 PfoI T'CCnGG_A Cuts at: 2487 2544 2732 2957 3246 2487 Size: 57 188 225 289 5350 Fragments arranged by size: 5350 289 225 188 57 PmeI GTTT'AAAC Cuts at: 3413 3413 Size: 6109 PmlI CAC'GTG Cuts at: 2869 2869 Size: 6109 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4178 4210 5691 5723 4178 Size: 32 1481 32 4564 Fragments arranged by size: 4564 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2408 2818 3358 2408 Size: 410 540 5159 Fragments arranged by size: 5159 540 410 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6109 PsiI TTA'TAA Cuts at: 3679 3679 Size: 6109 PspXI vC'TCGA_Gb Cuts at: 3331 3331 Size: 6109 PstI C_TGCA'G Cuts at: 830 2206 3461 830 Size: 1376 1255 3478 Fragments arranged by size: 3478 1376 1255 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6109 PvuII CAG'CTG Cuts at: 2312 2972 3241 2312 Size: 660 269 5180 Fragments arranged by size: 5180 660 269 SacI G_AGCT'C Cuts at: 2769 2769 Size: 6109 SalI G'TCGA_C Cuts at: 1936 3451 1936 Size: 1515 4594 SanDI GG'GwC_CC Cuts at: 3358 3358 Size: 6109 SapI GCTCTTCn'nnn_ Cuts at: 2369 2369 Size: 6109 SbfI CC_TGCA'GG Cuts at: 3461 3461 Size: 6109 ScaI AGT'ACT Cuts at: 4357 6069 4357 Size: 1712 4397 SfcI C'TryA_G Cuts at: 826 1045 2202 2749 3457 4592 5273 5464 826 Size: 219 1157 547 708 1135 681 191 1471 Fragments arranged by size: 1471 1157 1135 708 681 547 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 SgrAI Cr'CCGG_yG Cuts at: 1700 3295 1700 Size: 1595 4514 SmaI CCC'GGG Cuts at: 1872 2148 3436 1872 Size: 276 1288 4545 Fragments arranged by size: 4545 1288 276 SmlI C'TyrA_G Cuts at: 820 1017 1943 3331 4213 5114 5376 820 Size: 197 926 1388 882 901 262 1553 Fragments arranged by size: 1553 1388 926 901 882 262 197 SnaBI TAC'GTA Cuts at: 493 3900 493 Size: 3407 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6109 SphI G_CATG'C Cuts at: 1132 3217 3467 1132 Size: 2085 250 3774 Fragments arranged by size: 3774 2085 250 SspI AAT'ATT Cuts at: 5 52 3031 4033 5 Size: 47 2979 1002 2081 Fragments arranged by size: 2979 2081 1002 47 StuI AGG'CCT Cuts at: 1517 3256 1517 Size: 1739 4370 StyI C'CwwG_G Cuts at: 513 1065 2069 2934 2945 3548 513 Size: 552 1004 865 11 603 3074 Fragments arranged by size: 3074 1004 865 603 552 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2173 4136 4153 4306 4491 4936 325 Size: 1255 593 1963 17 153 185 445 1498 Fragments arranged by size: 1963 1498 1255 593 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4355 6067 96 Size: 274 80 33 51 1443 2378 1712 138 Fragments arranged by size: 2378 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2115 2147 6069 6101 2115 Size: 32 3922 32 2123 Fragments arranged by size: 3922 2123 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6109 XbaI T'CTAG_A Cuts at: 3445 3445 Size: 6109 XhoI C'TCGA_G Cuts at: 1943 3331 1943 Size: 1388 4721 XmaI C'CCGG_G Cuts at: 1870 2146 3434 1870 Size: 276 1288 4545 Fragments arranged by size: 4545 1288 276 XmnI GAAnn'nnTTC Cuts at: 2284 4238 2284 Size: 1954 4155 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3917 276 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I EcoNI FalI FspAI KasI NarI NruI PacI PspOMI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI