(Circular) (Six-base) MAPSORT of: pFN21AB6456.seq Check: 6342 from: 1 to: 5758 pFN21AB6456 5758 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3568 278 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2211 3101 1937 Size: 274 890 4594 Fragments arranged by size: 4594 890 274 Acc65I G'GTAC_C Cuts at: 3079 3079 Size: 5758 AclI AA'CG_TT Cuts at: 1563 3885 4258 1563 Size: 2322 373 3063 Fragments arranged by size: 3063 2322 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2046 2727 3821 5205 5473 1632 Size: 414 681 1094 1384 268 1917 Fragments arranged by size: 1917 1384 1094 681 414 268 AfeI AGC'GCT Cuts at: 2333 2333 Size: 5758 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5561 AflIII A'CryG_T Cuts at: 1203 4657 5634 1203 Size: 3454 977 1327 Fragments arranged by size: 3454 1327 977 AgeI A'CCGG_T Cuts at: 4589 4589 Size: 5758 AhdI GACnn_n'nnGTC Cuts at: 4487 4487 Size: 5758 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5758 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5758 AlwNI CAG_nnn'CTG Cuts at: 3501 5073 3501 Size: 1572 4186 ApaLI G'TGCA_C Cuts at: 3815 4971 3815 Size: 1156 4602 ApoI r'AATT_y Cuts at: 1493 2320 2385 2462 2968 3067 3293 3445 5554 Size: 827 65 77 506 99 226 152 2109 Cuts at: 5554 1493 Size: 1697 Fragments arranged by size: 2109 1697 827 506 226 152 99 77 65 AseI AT'TA_AT Cuts at: 160 1032 4312 160 Size: 872 3280 1606 Fragments arranged by size: 3280 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 AvaI C'yCGr_G Cuts at: 1870 1943 3083 1870 Size: 73 1140 4545 Fragments arranged by size: 4545 1140 73 BamHI G'GATC_C Cuts at: 3088 3455 3088 Size: 367 5391 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3079 4534 618 Size: 325 217 627 1292 1455 1842 Fragments arranged by size: 1842 1455 1292 627 325 217 BanII G_rGCy'C Cuts at: 1391 3077 1391 Size: 1686 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3028 928 Size: 971 1129 3658 Fragments arranged by size: 3658 1129 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3949 3983 1903 Size: 34 2012 34 3678 Fragments arranged by size: 3678 2012 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3650 4860 3650 Size: 1210 4548 BclI T'GATC_A Cuts at: 1548 1548 Size: 5758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4369 136 Size: 107 122 71 631 605 2697 1525 Fragments arranged by size: 2697 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5753 5753 Size: 5758 BlpI GC'TnA_GC Cuts at: 3175 3175 Size: 5758 Bme1580I G_kGCm'C Cuts at: 1790 3819 4975 1790 Size: 2029 1156 2573 Fragments arranged by size: 2573 2029 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5758 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3489 4447 442 Size: 416 53 83 399 24 2072 958 1753 Fragments arranged by size: 2072 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5758 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4418 1239 Size: 3179 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3883 4748 5046 3883 Size: 865 298 4595 Fragments arranged by size: 4595 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4421 882 Size: 617 2922 2219 Fragments arranged by size: 2922 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3549 493 Size: 711 2345 2702 Fragments arranged by size: 2702 2345 711 BsaBI GATnn'nnATC Cuts at: 5752 5752 Size: 5758 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3565 3947 4582 275 Size: 53 83 186 936 2032 382 635 1451 Fragments arranged by size: 2032 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4191 4589 4863 5010 5421 4191 Size: 398 274 147 411 4528 Fragments arranged by size: 4528 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5533 Fragments arranged by size: 5533 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5758 BseYI C'CCAG_C Cuts at: 4961 5457 4961 Size: 496 5262 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2369 1163 Size: 573 633 4552 Fragments arranged by size: 4552 633 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 3208 3969 4997 1540 Size: 275 186 33 1174 761 1028 2301 Fragments arranged by size: 2301 1174 1028 761 275 186 33 BsiHKAI G_wGCw'C Cuts at: 3819 3904 4975 3819 Size: 85 1071 4602 Fragments arranged by size: 4602 1071 85 BsmI GAATG_Cn' Cuts at: 3269 3362 3269 Size: 93 5665 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3077 3819 3904 4975 1391 Size: 399 1287 742 85 1071 2174 Fragments arranged by size: 2174 1287 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5421 5421 Size: 5758 BspHI T'CATG_A Cuts at: 3647 5377 3647 Size: 1730 4028 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2341 3113 844 Size: 1497 772 3489 Fragments arranged by size: 3489 1497 772 BsrBI CCG'CTC Cuts at: 1394 1823 3645 1394 Size: 429 1822 3507 Fragments arranged by size: 3507 1822 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4253 4427 65 Size: 1183 3005 174 1396 Fragments arranged by size: 3005 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2124 4402 4589 1700 Size: 110 314 2278 187 2869 Fragments arranged by size: 2869 2278 314 187 110 BsrGI T'GTAC_A Cuts at: 96 2415 96 Size: 2319 3439 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5758 BssSI C'ACGA_G Cuts at: 3818 4830 3818 Size: 1012 4746 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5758 BstBI TT'CG_AA Cuts at: 4562 4562 Size: 5758 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5758 BstYI r'GATC_y Cuts at: 1972 2224 2446 2603 3088 3455 3840 3857 5309 Size: 252 222 157 485 367 385 17 1452 Cuts at: 5309 5395 5407 5753 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 485 385 367 346 252 222 157 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4267 Fragments arranged by size: 4267 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2539 487 Size: 683 56 1313 3706 Fragments arranged by size: 3706 1313 683 56 BtsI GCAGTG_nn' Cuts at: 3274 4068 4088 3274 Size: 794 20 4944 Fragments arranged by size: 4944 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5758 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5723 DraI TTT'AAA Cuts at: 3062 3062 Size: 5758 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4765 809 Size: 507 3449 1802 Fragments arranged by size: 3449 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 3205 4094 Size: 54 1425 50 275 18 201 1174 889 Cuts at: 4094 5723 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1174 889 275 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2031 3205 1537 Size: 275 219 1174 4090 Fragments arranged by size: 4090 1174 275 219 EarI CTCTTCn'nnn_ Cuts at: 1353 2091 2199 2627 3688 1353 Size: 738 108 428 1061 3423 Fragments arranged by size: 3423 1061 738 428 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4345 4719 4865 387 Size: 1064 138 2756 374 146 1280 Fragments arranged by size: 2756 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2046 2727 3821 4418 5205 5473 1239 Size: 393 414 681 1094 597 787 268 1524 Fragments arranged by size: 1524 1094 787 681 597 414 393 268 EcoRI G'AATT_C Cuts at: 3067 3445 3067 Size: 378 5380 FspI TGC'GCA Cuts at: 4264 4264 Size: 5758 HaeII r_GCGC'y Cuts at: 1678 2335 4905 1678 Size: 657 2570 2531 Fragments arranged by size: 2570 2531 657 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2042 2071 2074 2528 Size: 32 135 32 467 3 29 3 454 Cuts at: 2528 2560 4399 4431 4473 4505 1373 Size: 32 1839 32 42 32 2626 Fragments arranged by size: 2626 1839 467 454 135 42 32 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2212 3102 3348 669 Size: 988 281 274 890 246 3079 Fragments arranged by size: 3079 988 890 281 274 246 HindIII A'AGCT_T Cuts at: 748 2243 748 Size: 1495 4263 HpaI GTT'AAC Cuts at: 3348 3348 Size: 5758 KpnI G_GTAC'C Cuts at: 3083 3083 Size: 5758 MfeI C'AATT_G Cuts at: 2478 3357 2478 Size: 879 4879 MluI A'CGCG_T Cuts at: 5634 5634 Size: 5758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4872 5056 1305 Size: 3567 184 2007 Fragments arranged by size: 3567 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3936 Fragments arranged by size: 3936 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2831 2846 3716 4075 4234 518 Size: 648 1665 15 870 359 159 2042 Fragments arranged by size: 2042 1665 870 648 359 159 15 MspA1I CmG'CkG Cuts at: 1606 3174 3851 4999 5244 1606 Size: 1568 677 1148 245 2120 Fragments arranged by size: 2120 1568 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4267 Fragments arranged by size: 4267 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5758 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5758 NotI GC'GGCC_GC Cuts at: 3205 3205 Size: 5758 NspI r_CATG'y Cuts at: 1132 1229 3116 4661 1132 Size: 97 1887 1545 2229 Fragments arranged by size: 2229 1887 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5758 PciI A'CATG_T Cuts at: 4657 4657 Size: 5758 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5758 PmeI GTTT'AAAC Cuts at: 3062 3062 Size: 5758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3827 3859 5340 5372 3827 Size: 32 1481 32 4213 Fragments arranged by size: 4213 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5758 PsiI TTA'TAA Cuts at: 3328 3328 Size: 5758 PstI C_TGCA'G Cuts at: 830 3110 830 Size: 2280 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5758 SalI G'TCGA_C Cuts at: 1936 2210 3100 1936 Size: 274 890 4594 Fragments arranged by size: 4594 890 274 SapI GCTCTTCn'nnn_ Cuts at: 2091 2091 Size: 5758 SbfI CC_TGCA'GG Cuts at: 3110 3110 Size: 5758 ScaI AGT'ACT Cuts at: 2264 4006 5718 2264 Size: 1742 1712 2304 Fragments arranged by size: 2304 1742 1712 SfcI C'TryA_G Cuts at: 826 1045 2580 3106 4241 4922 5113 826 Size: 219 1535 526 1135 681 191 1471 Fragments arranged by size: 1535 1471 1135 681 526 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5758 SmaI CCC'GGG Cuts at: 1872 3085 1872 Size: 1213 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3862 4763 5025 820 Size: 197 926 1919 901 262 1553 Fragments arranged by size: 1919 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3549 493 Size: 3056 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5758 SphI G_CATG'C Cuts at: 1132 3116 1132 Size: 1984 3774 SspI AAT'ATT Cuts at: 5 52 3682 5 Size: 47 3630 2081 Fragments arranged by size: 3630 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5758 StyI C'CwwG_G Cuts at: 513 1065 2004 3042 3197 513 Size: 552 939 1038 155 3074 Fragments arranged by size: 3074 1038 939 552 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3785 3802 3955 4140 4585 325 Size: 1255 2205 17 153 185 445 1498 Fragments arranged by size: 2205 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2262 2415 2856 Size: 274 80 33 51 1443 285 153 441 Cuts at: 2856 4004 5716 96 Size: 1148 1712 138 Fragments arranged by size: 1712 1443 1148 441 285 274 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5718 5750 5718 Size: 32 5726 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5758 XbaI T'CTAG_A Cuts at: 3094 3094 Size: 5758 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5758 XmaI C'CCGG_G Cuts at: 1870 3083 1870 Size: 1213 4545 XmnI GAAnn'nnTTC Cuts at: 2116 2264 2691 3887 2116 Size: 148 427 1196 3987 Fragments arranged by size: 3987 1196 427 148 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3566 276 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI