Restriction map for
pFN21AB8560
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsoBI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FauI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HpaI HpaII KasI
KpnI KspI MaeII MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI Tth111I Van91I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AccI 2377 GTCGAC GT^[AC][GT]AC
AciI 1954 GCGG CCGC(-3/-1)
BanII 1757 GAGCTC G[GA]GC[CT]^C
BciVI 1996 GTATCC GTATCC(6/5)
BclI 1164 TGATCA T^GATCA
BglII 2330 AGATCT A^GATCT
BseRI 632 GAGGAG GAGGAG(10/8)
BsmBI 3 GAGACG CGTCTC(1/5)
BsmI 654 GCATTC GAATGC(1/-1)
BspHI 2469 TCATGA T^CATGA
BspMI 614 GCAGGT ACCTGC(4/8)
BstF5I 852 GGATG GGATG(2/0)
DraII 1038 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 1038 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 335 CCAAGG C^C[AT][AT]GG
FbaI 1164 TGATCA T^GATCA
FokI 852 GGATG GGATG(9/13)
HaeIII 541 GGCC GG^CC
HgaI 2043 GCGTC GACGC(5/10)
HindIII 1092 AAGCTT A^AGCTT
PpuMI 1038 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 2469 TCATGA T^CATGA
SacI 1757 GAGCTC GAGCT^C
SalI 2377 GTCGAC G^TCGAC
SapI 1541 GAAGAGC GCTCTTC(1/4)
SmlI 2714 CTCAAG C^T[CT][GA]AG
StyI 335 CCAAGG C^C[AT][AT]GG
SwaI 2642 ATTTAAAT ATTT^AAAT
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# TWO CUT ENZYMES ( 31 enzymes) #
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AlwNI 1704 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2231 CAGCCACTG
AseI 145 ATTAAT AT^TAAT
1416 ATTAAT
AsnI 145 ATTAAT AT^TAAT
1416 ATTAAT
AspHI 1655 GAGCAC G[AT]GC[AT]^C
1757 GAGCTC
BbsI 993 GAAGAC GAAGAC(2/6)
1068 GAAGAC
BmyI 1655 GAGCAC G[AGT]GC[ACT]^C
1757 GAGCTC
BpuAI 993 GAAGAC GAAGAC(2/6)
1068 GAAGAC
BsaBI 805 GATAAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2221 GATTGGAATC
BsaJI 335 CCAAGG C^C[ACGT][ACGT]GG
1247 CCCTGG
BsiHKAI 1655 GAGCAC G[AT]GC[AT]^C
1757 GAGCTC
BsiYI 843 CCCTCTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1042 CCCTATGGAGG
BslI 843 CCCTCTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1042 CCCTATGGAGG
BsmAI 3 GAGAC GTCTC(1/5)
2539 GTCTC
Bsp1286I 1655 GAGCAC G[AGT]GC[ACT]^C
1757 GAGCTC
Bsp1407I 2286 TGTACA T^GTACA
2491 TGTACA
BsrGI 2286 TGTACA T^GTACA
2491 TGTACA
Cac8I 1052 GCAAGC GC[ACGT]^[ACGT]GC
1865 GCTTGC
DraI 192 TTTAAA TTT^AAA
2643 TTTAAA
EcoRI 36 GAATTC G^AATTC
1186 GAATTC
HincII 2123 GTCAAC GT[CT]^[GA]AC
2377 GTCGAC
HindII 2123 GTCAAC GT[CT]^[GA]AC
2377 GTCGAC
MamI 805 GATAAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2221 GATTGGAATC
MspA1I 1547 CAGCTG C[AC]G^C[GT]G
2608 CAGCTG
MwoI 752 GCAAACCACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
964 GCTATTCTTGC
PshBI 145 ATTAAT AT^TAAT
1416 ATTAAT
PstI 11 CTGCAG CTGCA^G
1027 CTGCAG
PvuII 1547 CAGCTG CAG^CTG
2608 CAGCTG
ScaI 214 AGTACT AGT^ACT
640 AGTACT
SfaNI 430 GATGC GCATC(5/9)
1299 GCATC
SspBI 2286 TGTACA T^GTACA
2491 TGTACA
XbaI 2254 TCTAGA T^CTAGA
2542 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AvaII BbvI BfaI BpmI
BsmFI BsrDI BsrI BssKI BstNI
BstYI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfcI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI