(Circular) (Six-base) MAPSORT of: pFN21AB8560.seq Check: 964 from: 1 to: 7435 pFN21AB8560 7435 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2610 2610 Size: 7435 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5245 278 Size: 53 83 186 936 3709 2468 Fragments arranged by size: 3709 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4383 4778 1937 Size: 2446 395 4594 Fragments arranged by size: 4594 2446 395 Acc65I G'GTAC_C Cuts at: 4756 4756 Size: 7435 AclI AA'CG_TT Cuts at: 1563 5562 5935 1563 Size: 3999 373 3063 Fragments arranged by size: 3999 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2593 4637 5498 6882 7150 1632 Size: 961 2044 861 1384 268 1917 Fragments arranged by size: 2044 1917 1384 961 861 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7238 AflIII A'CryG_T Cuts at: 1203 6334 7311 1203 Size: 5131 977 1327 Fragments arranged by size: 5131 1327 977 AgeI A'CCGG_T Cuts at: 6266 6266 Size: 7435 AhdI GACnn_n'nnGTC Cuts at: 6164 6164 Size: 7435 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7435 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7435 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4494 4526 4494 Size: 32 7403 AlwNI CAG_nnn'CTG Cuts at: 3714 4241 5178 6750 3714 Size: 527 937 1572 4399 Fragments arranged by size: 4399 1572 937 527 ApaLI G'TGCA_C Cuts at: 5492 6648 5492 Size: 1156 6279 ApoI r'AATT_y Cuts at: 1493 2041 2060 2476 2516 2785 3122 3191 3275 Size: 548 19 416 40 269 337 69 84 Cuts at: 3275 3410 3694 4435 4535 4744 4970 5122 7231 Size: 135 284 741 100 209 226 152 2109 Cuts at: 7231 1493 Size: 1697 Fragments arranged by size: 2109 1697 741 548 416 337 284 269 226 209 152 135 100 84 69 40 19 AseI AT'TA_AT Cuts at: 160 1032 2151 3422 5989 160 Size: 872 1119 1271 2567 1606 Fragments arranged by size: 2567 1606 1271 1119 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7435 AvaI C'yCGr_G Cuts at: 1870 1943 4760 1870 Size: 73 2817 4545 Fragments arranged by size: 4545 2817 73 BamHI G'GATC_C Cuts at: 4765 5132 4765 Size: 367 7068 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4756 6211 618 Size: 325 217 627 2969 1455 1842 Fragments arranged by size: 2969 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3766 4754 1391 Size: 2375 988 4072 Fragments arranged by size: 4072 2375 988 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3005 3080 928 Size: 971 1106 75 5283 Fragments arranged by size: 5283 1106 971 75 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5626 5660 1903 Size: 34 3689 34 3678 Fragments arranged by size: 3689 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4012 5327 6537 4012 Size: 1315 1210 4910 Fragments arranged by size: 4910 1315 1210 BclI T'GATC_A Cuts at: 1548 3169 1548 Size: 1621 5814 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6046 136 Size: 107 122 71 631 605 4374 1525 Fragments arranged by size: 4374 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4335 7430 4335 Size: 3095 4340 BlpI GC'TnA_GC Cuts at: 4852 4852 Size: 7435 Bme1580I G_kGCm'C Cuts at: 1790 5496 6652 1790 Size: 3706 1156 2573 Fragments arranged by size: 3706 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7435 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5166 6124 442 Size: 416 53 83 399 24 3749 958 1753 Fragments arranged by size: 3749 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7435 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2055 2583 4659 6095 1239 Size: 816 528 2076 1436 2579 Fragments arranged by size: 2579 2076 1436 816 528 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4704 5560 6425 6723 4704 Size: 856 865 298 5416 Fragments arranged by size: 5416 865 856 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6098 882 Size: 617 4599 2219 Fragments arranged by size: 4599 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5226 493 Size: 711 4022 2702 Fragments arranged by size: 4022 2702 711 BsaBI GATnn'nnATC Cuts at: 2814 4230 7429 2814 Size: 1416 3199 2820 Fragments arranged by size: 3199 2820 1416 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5242 5624 6259 275 Size: 53 83 186 936 3709 382 635 1451 Fragments arranged by size: 3709 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5868 6266 6540 6687 7098 5868 Size: 398 274 147 411 6205 Fragments arranged by size: 6205 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3370 3400 4261 4291 4494 Size: 30 165 30 1967 30 861 30 203 Cuts at: 4494 4524 1178 Size: 30 4089 Fragments arranged by size: 4089 1967 861 203 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2652 1186 Size: 1466 5969 BseYI C'CCAG_C Cuts at: 4611 6638 7134 4611 Size: 2027 496 4912 Fragments arranged by size: 4912 2027 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6862 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4885 5646 6674 1540 Size: 275 186 2884 761 1028 2301 Fragments arranged by size: 2884 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3664 3766 5496 5581 6652 3664 Size: 102 1730 85 1071 4447 Fragments arranged by size: 4447 1730 1071 102 85 BsmI GAATG_Cn' Cuts at: 2659 4946 5039 2659 Size: 2287 93 5055 Fragments arranged by size: 5055 2287 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2002 2002 Size: 7435 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3664 3766 4754 5496 5581 6652 1391 Size: 399 1874 102 988 742 85 1071 2174 Fragments arranged by size: 2174 1874 1071 988 742 399 102 85 BspEI T'CCGG_A Cuts at: 7098 7098 Size: 7435 BspHI T'CATG_A Cuts at: 4474 5324 7054 4474 Size: 850 1730 4855 Fragments arranged by size: 4855 1730 850 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2610 4790 844 Size: 1766 2180 3489 Fragments arranged by size: 3489 2180 1766 BsrBI CCG'CTC Cuts at: 1394 1823 5322 1394 Size: 429 3499 3507 Fragments arranged by size: 3507 3499 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3002 3014 4260 4628 5930 6104 65 Size: 1183 1754 12 1246 368 1302 174 1396 Fragments arranged by size: 1754 1396 1302 1246 1183 368 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 6079 6266 1700 Size: 110 4269 187 2869 Fragments arranged by size: 4269 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4291 4496 96 Size: 4195 205 3035 Fragments arranged by size: 4195 3035 205 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7435 BssSI C'ACGA_G Cuts at: 5495 6507 5495 Size: 1012 6423 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7435 BstBI TT'CG_AA Cuts at: 6239 6239 Size: 7435 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7435 BstYI r'GATC_y Cuts at: 1972 2293 4199 4335 4660 4765 5132 5517 5534 Size: 321 1906 136 325 105 367 385 17 Cuts at: 5534 6986 7072 7084 7430 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1906 1452 385 367 346 325 321 136 105 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6883 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6696 Fragments arranged by size: 6696 683 56 BtsI GCAGTG_nn' Cuts at: 2257 2340 2620 3041 4951 5745 5765 2257 Size: 83 280 421 1910 794 20 3927 Fragments arranged by size: 3927 1910 794 421 280 83 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7435 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7400 DraI TTT'AAA Cuts at: 2199 4650 4739 2199 Size: 2451 89 4895 Fragments arranged by size: 4895 2451 89 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6442 809 Size: 507 5126 1802 Fragments arranged by size: 5126 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4882 5771 7400 Size: 54 1425 50 275 18 3052 889 1629 Cuts at: 7400 8 Size: 43 Fragments arranged by size: 3052 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4882 1537 Size: 275 3070 4090 Fragments arranged by size: 4090 3070 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2094 2346 2494 2535 3541 4141 5365 1353 Size: 741 252 148 41 1006 600 1224 3423 Fragments arranged by size: 3423 1224 1006 741 600 252 148 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6022 6396 6542 387 Size: 1064 138 4433 374 146 1280 Fragments arranged by size: 4433 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3764 3764 Size: 7435 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2583 2593 4637 4659 5498 6095 Size: 393 423 528 10 2044 22 839 597 Cuts at: 6095 6882 7150 1239 Size: 787 268 1524 Fragments arranged by size: 2044 1524 839 787 597 528 423 393 268 22 10 EcoO109I rG'GnC_Cy Cuts at: 3044 3044 Size: 7435 EcoRI G'AATT_C Cuts at: 2041 3191 4744 5122 2041 Size: 1150 1553 378 4354 Fragments arranged by size: 4354 1553 1150 378 FspI TGC'GCA Cuts at: 5941 5941 Size: 7435 HaeII r_GCGC'y Cuts at: 1678 6582 1678 Size: 4904 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3567 3599 4173 4205 4261 Size: 32 135 32 1995 32 574 32 56 Cuts at: 4261 4293 6076 6108 6150 6182 1373 Size: 32 1783 32 42 32 2626 Fragments arranged by size: 2626 1995 1783 574 135 56 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4130 4384 4779 5025 669 Size: 988 281 2192 254 395 246 3079 Fragments arranged by size: 3079 2192 988 395 281 254 246 HindIII A'AGCT_T Cuts at: 748 3097 748 Size: 2349 5086 HpaI GTT'AAC Cuts at: 5025 5025 Size: 7435 KpnI G_GTAC'C Cuts at: 4760 4760 Size: 7435 MfeI C'AATT_G Cuts at: 5034 5034 Size: 7435 MluI A'CGCG_T Cuts at: 7311 7311 Size: 7435 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3711 4029 4509 6549 6733 1305 Size: 2406 318 480 2040 184 2007 Fragments arranged by size: 2406 2040 2007 480 318 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5613 Fragments arranged by size: 5613 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3007 4056 4518 5393 5752 5911 518 Size: 648 1841 1049 462 875 359 159 2042 Fragments arranged by size: 2042 1841 1049 875 648 462 359 159 MspA1I CmG'CkG Cuts at: 1606 3554 4615 4851 5528 6676 6921 1606 Size: 1948 1061 236 677 1148 245 2120 Fragments arranged by size: 2120 1948 1148 1061 677 245 236 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6883 NdeI CA'TA_TG Cuts at: 387 387 Size: 7435 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7435 NotI GC'GGCC_GC Cuts at: 4882 4882 Size: 7435 NspI r_CATG'y Cuts at: 1132 1229 4793 6338 1132 Size: 97 3564 1545 2229 Fragments arranged by size: 3564 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7435 PciI A'CATG_T Cuts at: 6334 6334 Size: 7435 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7435 PfoI T'CCnGG_A Cuts at: 2071 3439 2071 Size: 1368 6067 PmeI GTTT'AAAC Cuts at: 4739 4739 Size: 7435 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4494 4526 5504 5536 7017 7049 4494 Size: 32 978 32 1481 32 4880 Fragments arranged by size: 4880 1481 978 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3044 3044 Size: 7435 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7435 PsiI TTA'TAA Cuts at: 2514 3608 5005 2514 Size: 1094 1397 4944 Fragments arranged by size: 4944 1397 1094 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2704 2736 2704 Size: 32 7403 PstI C_TGCA'G Cuts at: 830 2020 3036 4787 830 Size: 1190 1016 1751 3478 Fragments arranged by size: 3478 1751 1190 1016 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7435 PvuII CAG'CTG Cuts at: 3554 4615 3554 Size: 1061 6374 SacI G_AGCT'C Cuts at: 3766 3766 Size: 7435 SalI G'TCGA_C Cuts at: 1936 4382 4777 1936 Size: 2446 395 4594 Fragments arranged by size: 4594 2446 395 SanDI GG'GwC_CC Cuts at: 3044 3044 Size: 7435 SapI GCTCTTCn'nnn_ Cuts at: 3541 3541 Size: 7435 SbfI CC_TGCA'GG Cuts at: 4787 4787 Size: 7435 ScaI AGT'ACT Cuts at: 2221 2647 5683 7395 2221 Size: 426 3036 1712 2261 Fragments arranged by size: 3036 2261 1712 426 SfcI C'TryA_G Cuts at: 826 1045 2016 3032 3336 3665 3935 4314 4783 Size: 219 971 1016 304 329 270 379 469 Cuts at: 4783 5918 6599 6790 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1016 971 681 469 379 329 304 270 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7435 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7435 SmaI CCC'GGG Cuts at: 1872 4762 1872 Size: 2890 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4719 5539 6440 6702 820 Size: 197 926 2776 820 901 262 1553 Fragments arranged by size: 2776 1553 926 901 820 262 197 SnaBI TAC'GTA Cuts at: 493 5226 493 Size: 4733 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7435 SphI G_CATG'C Cuts at: 1132 4793 1132 Size: 3661 3774 SspI AAT'ATT Cuts at: 5 52 2261 2378 3447 5359 5 Size: 47 2209 117 1069 1912 2081 Fragments arranged by size: 2209 2081 1912 1069 117 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7435 StyI C'CwwG_G Cuts at: 513 1065 2340 4874 513 Size: 552 1275 2534 3074 Fragments arranged by size: 3074 2534 1275 552 SwaI ATTT'AAAT Cuts at: 4650 4650 Size: 7435 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2576 5462 5479 5632 5817 6262 325 Size: 1255 996 2886 17 153 185 445 1498 Fragments arranged by size: 2886 1498 1255 996 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2219 2645 4291 Size: 274 80 33 51 1443 242 426 1646 Cuts at: 4291 4496 5681 7393 96 Size: 205 1185 1712 138 Fragments arranged by size: 1712 1646 1443 1185 426 274 242 205 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3087 3119 7395 7427 3087 Size: 32 4276 32 3095 Fragments arranged by size: 4276 3095 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7435 XbaI T'CTAG_A Cuts at: 4259 4547 4771 4259 Size: 288 224 6923 Fragments arranged by size: 6923 288 224 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7435 XmaI C'CCGG_G Cuts at: 1870 4760 1870 Size: 2890 4545 XmnI GAAnn'nnTTC Cuts at: 2045 2684 2744 2843 4407 4509 5564 2045 Size: 639 60 99 1564 102 1055 3916 Fragments arranged by size: 3916 1564 1055 639 102 99 60 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5243 276 Size: 53 83 186 936 3709 2468 Fragments arranged by size: 3709 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SexAI SfiI SfoI SrfI XcmI