Restriction map for
pFN21AB8560
(Linear) (Six-base) MAPSORT of: KIBB8560.seq Check: 7428 from: 1 to: 2730
KIBB8560.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 605 2730
Size: 605 2125
AccI GT'mk_AC
Cuts at: 0 2378 2730
Size: 2378 352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 2632 2730
Size: 588 2044 98
Fragments arranged by size:
2044 588 98
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2489 2521 2730
Size: 2489 32 209
Fragments arranged by size:
2489 209 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1709 2236 2730
Size: 1709 527 494
Fragments arranged by size:
1709 527 494
ApoI r'AATT_y
Cuts at: 0 36 55 471 511 780 1117 1186 1270
Size: 36 19 416 40 269 337 69 84
Cuts at: 1270 1405 1689 2430 2530 2730
Size: 135 284 741 100 200
Fragments arranged by size:
741 416 337 284 269 200 135 100
84 69 40 36 19
AseI AT'TA_AT
Cuts at: 0 146 1417 2730
Size: 146 1271 1313
Fragments arranged by size:
1313 1271 146
BanII G_rGCy'C
Cuts at: 0 1761 2730
Size: 1761 969
BbsI GAAGACnn'nnnn_
Cuts at: 0 1000 1075 2730
Size: 1000 75 1655
Fragments arranged by size:
1655 1000 75
BciVI GTATCCnnnnn_n'
Cuts at: 0 2007 2730
Size: 2007 723
BclI T'GATC_A
Cuts at: 0 1164 2730
Size: 1164 1566
BglII A'GATC_T
Cuts at: 0 2330 2730
Size: 2330 400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 578 2654 2730
Size: 50 528 2076 76
Fragments arranged by size:
2076 528 76 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2699 2730
Size: 2699 31
BsaBI GATnn'nnATC
Cuts at: 0 809 2225 2730
Size: 809 1416 505
Fragments arranged by size:
1416 809 505
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1365 1395 2256 2286 2489 2519 2730
Size: 1365 30 861 30 203 30 211
Fragments arranged by size:
1365 861 211 203 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 647 2730
Size: 647 2083
BseYI C'CCAG_C
Cuts at: 0 2606 2730
Size: 2606 124
BsiHKAI G_wGCw'C
Cuts at: 0 1659 1761 2730
Size: 1659 102 969
Fragments arranged by size:
1659 969 102
BsmI GAATG_Cn'
Cuts at: 0 654 2730
Size: 654 2076
Bsp1286I G_dGCh'C
Cuts at: 0 1659 1761 2730
Size: 1659 102 969
Fragments arranged by size:
1659 969 102
BspHI T'CATG_A
Cuts at: 0 2469 2730
Size: 2469 261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 605 2730
Size: 605 2125
BsrDI GCAATG_nn'
Cuts at: 0 997 1009 2255 2623 2730
Size: 997 12 1246 368 107
Fragments arranged by size:
1246 997 368 107 12
BsrGI T'GTAC_A
Cuts at: 0 2286 2491 2730
Size: 2286 205 239
Fragments arranged by size:
2286 239 205
BstYI r'GATC_y
Cuts at: 0 288 2194 2330 2655 2730
Size: 288 1906 136 325 75
Fragments arranged by size:
1906 325 288 136 75
BtsI GCAGTG_nn'
Cuts at: 0 252 335 615 1036 2730
Size: 252 83 280 421 1694
Fragments arranged by size:
1694 421 280 252 83
DraI TTT'AAA
Cuts at: 0 194 2645 2730
Size: 194 2451 85
Fragments arranged by size:
2451 194 85
EarI CTCTTCn'nnn_
Cuts at: 0 89 341 489 530 1536 2136 2730
Size: 89 252 148 41 1006 600 594
Fragments arranged by size:
1006 600 594 252 148 89 41
EcoICRI GAG'CTC
Cuts at: 0 1759 2730
Size: 1759 971
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 578 588 2632 2654 2730
Size: 50 528 10 2044 22 76
Fragments arranged by size:
2044 528 76 50 22 10
EcoO109I rG'GnC_Cy
Cuts at: 0 1039 2730
Size: 1039 1691
EcoRI G'AATT_C
Cuts at: 0 36 1186 2730
Size: 36 1150 1544
Fragments arranged by size:
1544 1150 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1562 1594 2168 2200 2256 2288 2730
Size: 1562 32 574 32 56 32 442
Fragments arranged by size:
1562 574 442 56 32 32 32
HincII GTy'rAC
Cuts at: 0 2125 2379 2730
Size: 2125 254 351
Fragments arranged by size:
2125 351 254
HindIII A'AGCT_T
Cuts at: 0 1092 2730
Size: 1092 1638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1706 2024 2504 2730
Size: 1706 318 480 226
Fragments arranged by size:
1706 480 318 226
MslI CAynn'nnrTG
Cuts at: 0 1002 2051 2513 2730
Size: 1002 1049 462 217
Fragments arranged by size:
1049 1002 462 217
MspA1I CmG'CkG
Cuts at: 0 1549 2610 2730
Size: 1549 1061 120
Fragments arranged by size:
1549 1061 120
PfoI T'CCnGG_A
Cuts at: 0 66 1434 2730
Size: 66 1368 1296
Fragments arranged by size:
1368 1296 66
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2489 2521 2730
Size: 2489 32 209
Fragments arranged by size:
2489 209 32
PpuMI rG'GwC_Cy
Cuts at: 0 1039 2730
Size: 1039 1691
PsiI TTA'TAA
Cuts at: 0 509 1603 2730
Size: 509 1094 1127
Fragments arranged by size:
1127 1094 509
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 699 731 2730
Size: 699 32 1999
Fragments arranged by size:
1999 699 32
PstI C_TGCA'G
Cuts at: 0 15 1031 2730
Size: 15 1016 1699
Fragments arranged by size:
1699 1016 15
PvuII CAG'CTG
Cuts at: 0 1549 2610 2730
Size: 1549 1061 120
Fragments arranged by size:
1549 1061 120
SacI G_AGCT'C
Cuts at: 0 1761 2730
Size: 1761 969
SalI G'TCGA_C
Cuts at: 0 2377 2730
Size: 2377 353
SanDI GG'GwC_CC
Cuts at: 0 1039 2730
Size: 1039 1691
SapI GCTCTTCn'nnn_
Cuts at: 0 1536 2730
Size: 1536 1194
ScaI AGT'ACT
Cuts at: 0 216 642 2730
Size: 216 426 2088
Fragments arranged by size:
2088 426 216
SfcI C'TryA_G
Cuts at: 0 11 1027 1331 1660 1930 2309 2730
Size: 11 1016 304 329 270 379 421
Fragments arranged by size:
1016 421 379 329 304 270 11
SmlI C'TyrA_G
Cuts at: 0 2714 2730
Size: 2714 16
SspI AAT'ATT
Cuts at: 0 256 373 1442 2730
Size: 256 117 1069 1288
Fragments arranged by size:
1288 1069 256 117
StyI C'CwwG_G
Cuts at: 0 335 2730
Size: 335 2395
SwaI ATTT'AAAT
Cuts at: 0 2645 2730
Size: 2645 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 571 2730
Size: 571 2159
TatI w'GTAC_w
Cuts at: 0 214 640 2286 2491 2730
Size: 214 426 1646 205 239
Fragments arranged by size:
1646 426 239 214 205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1082 1114 2730
Size: 1082 32 1616
Fragments arranged by size:
1616 1082 32
XbaI T'CTAG_A
Cuts at: 0 2254 2542 2730
Size: 2254 288 188
Fragments arranged by size:
2254 288 188
XmnI GAAnn'nnTTC
Cuts at: 0 40 679 739 838 2402 2504 2730
Size: 40 639 60 99 1564 102 226
Fragments arranged by size:
1564 639 226 102 99 60 40
Enzymes that do cut:
AarI AccI AcuI AloI AlwNI ApoI AseI BanII
BbsI BciVI BclI BglII BpmI BpuEI BsaBI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrGI BstYI BtsI DraI EarI EcoICRI Eco57MI EcoO109I
EcoRI Hin4I HincII HindIII MmeI MslI MspA1I PfoI
PpiI PpuMI PsiI PsrI PstI PvuII SacI SalI
SanDI SapI ScaI SfcI SmlI SspI StyI SwaI
TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaHI BsaWI BsgI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI
EaeI EagI EciI EcoNI EcoRV FalI FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PshAI PspOMI PspXI PvuI RsrII SacII SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI Tth111I XcmI XhoI XmaI ZraI