(Circular) (Six-base) MAPSORT of: pFN21AB8554.seq Check: 1735 from: 1 to: 7429 pFN21AB8554 7429 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4382 4382 Size: 7429 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5239 278 Size: 53 83 186 936 3703 2468 Fragments arranged by size: 3703 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4367 4772 1937 Size: 2430 405 4594 Fragments arranged by size: 4594 2430 405 Acc65I G'GTAC_C Cuts at: 4750 4750 Size: 7429 AclI AA'CG_TT Cuts at: 1563 5556 5929 1563 Size: 3993 373 3063 Fragments arranged by size: 3993 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2225 3837 4087 4467 5492 6876 7144 1632 Size: 593 1612 250 380 1025 1384 268 1917 Fragments arranged by size: 1917 1612 1384 1025 593 380 268 250 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7232 AflIII A'CryG_T Cuts at: 1203 2316 6328 7305 1203 Size: 1113 4012 977 1327 Fragments arranged by size: 4012 1327 1113 977 AgeI A'CCGG_T Cuts at: 6260 6260 Size: 7429 AhdI GACnn_n'nnGTC Cuts at: 6158 6158 Size: 7429 AleI CACnn'nnGTG Cuts at: 1166 3803 1166 Size: 2637 4792 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7429 AlwNI CAG_nnn'CTG Cuts at: 5172 6744 5172 Size: 1572 5857 ApaLI G'TGCA_C Cuts at: 5486 6642 5486 Size: 1156 6273 ApoI r'AATT_y Cuts at: 1493 2786 3086 3100 3352 4738 4964 5116 7225 Size: 1293 300 14 252 1386 226 152 2109 Cuts at: 7225 1493 Size: 1697 Fragments arranged by size: 2109 1697 1386 1293 300 252 226 152 14 AseI AT'TA_AT Cuts at: 160 1032 2372 5983 160 Size: 872 1340 3611 1606 Fragments arranged by size: 3611 1606 1340 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7429 AvaI C'yCGr_G Cuts at: 1870 1943 2583 2719 2829 3177 4754 1870 Size: 73 640 136 110 348 1577 4545 Fragments arranged by size: 4545 1577 640 348 136 110 73 AvrII C'CTAG_G Cuts at: 3967 3967 Size: 7429 BamHI G'GATC_C Cuts at: 2073 2939 4153 4759 5126 2073 Size: 866 1214 606 367 4376 Fragments arranged by size: 4376 1214 866 606 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2188 3760 4750 6205 618 Size: 325 217 627 401 1572 990 1455 1842 Fragments arranged by size: 1842 1572 1455 990 627 401 325 217 BanII G_rGCy'C Cuts at: 1391 3520 4748 1391 Size: 2129 1228 4072 Fragments arranged by size: 4072 2129 1228 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3510 3955 928 Size: 971 1611 445 4402 Fragments arranged by size: 4402 1611 971 445 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5620 5654 1903 Size: 34 3683 34 3678 Fragments arranged by size: 3683 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3275 4031 4259 4283 5321 6531 3275 Size: 756 228 24 1038 1210 4173 Fragments arranged by size: 4173 1210 1038 756 228 24 BclI T'GATC_A Cuts at: 1548 4095 1548 Size: 2547 4882 BfrBI ATG'CAT Cuts at: 2534 2534 Size: 7429 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6040 136 Size: 107 122 71 631 605 4368 1525 Fragments arranged by size: 4368 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2715 3268 7424 2715 Size: 553 4156 2720 Fragments arranged by size: 4156 2720 553 BlpI GC'TnA_GC Cuts at: 4846 4846 Size: 7429 Bme1580I G_kGCm'C Cuts at: 1790 5490 6646 1790 Size: 3700 1156 2573 Fragments arranged by size: 3700 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7429 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2651 4414 5160 Size: 416 53 83 399 24 1234 1763 746 Cuts at: 5160 6118 442 Size: 958 1753 Fragments arranged by size: 1763 1753 1234 958 746 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7429 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 2802 3185 6089 1239 Size: 1013 550 383 2904 2579 Fragments arranged by size: 2904 2579 1013 550 383 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4208 5554 6419 6717 4208 Size: 1346 865 298 4920 Fragments arranged by size: 4920 1346 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2159 3679 6092 882 Size: 617 660 1520 2413 2219 Fragments arranged by size: 2413 2219 1520 660 617 BsaAI yAC'GTr Cuts at: 493 1204 2319 2768 3874 5220 493 Size: 711 1115 449 1106 1346 2702 Fragments arranged by size: 2702 1346 1115 1106 711 449 BsaBI GATnn'nnATC Cuts at: 4439 7423 4439 Size: 2984 4445 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2451 3542 5236 5618 Size: 53 83 186 936 918 1091 1694 382 Cuts at: 5618 6253 275 Size: 635 1451 Fragments arranged by size: 1694 1451 1091 936 918 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5862 6260 6534 6681 7092 5862 Size: 398 274 147 411 6199 Fragments arranged by size: 6199 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4122 4152 1178 Size: 30 165 30 2719 30 4455 Fragments arranged by size: 4455 2719 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2984 3110 4579 1186 Size: 1798 126 1469 4036 Fragments arranged by size: 4036 1798 1469 126 BseYI C'CCAG_C Cuts at: 6632 7128 6632 Size: 496 6933 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2384 3236 1163 Size: 573 648 852 5356 Fragments arranged by size: 5356 852 648 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4879 5640 6668 1540 Size: 275 186 2878 761 1028 2301 Fragments arranged by size: 2878 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2324 3879 4224 4724 5490 5575 6646 2324 Size: 1555 345 500 766 85 1071 3107 Fragments arranged by size: 3107 1555 1071 766 500 345 85 BsmI GAATG_Cn' Cuts at: 3742 4940 5033 3742 Size: 1198 93 6138 Fragments arranged by size: 6138 1198 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2324 3520 3879 4224 4724 4748 5490 Size: 399 534 1196 359 345 500 24 742 Cuts at: 5490 5575 6646 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1196 1071 742 534 500 399 359 345 85 24 BspEI T'CCGG_A Cuts at: 7092 7092 Size: 7429 BspHI T'CATG_A Cuts at: 5318 7048 5318 Size: 1730 5699 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4382 4784 844 Size: 3538 402 3489 Fragments arranged by size: 3538 3489 402 BsrBI CCG'CTC Cuts at: 1394 1823 2694 4716 5316 1394 Size: 429 871 2022 600 3507 Fragments arranged by size: 3507 2022 871 600 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2742 5924 6098 65 Size: 1183 1494 3182 174 1396 Fragments arranged by size: 3182 1494 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6073 6260 1700 Size: 110 4263 187 2869 Fragments arranged by size: 4263 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4437 96 Size: 4341 3088 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7429 BssSI C'ACGA_G Cuts at: 5489 6501 5489 Size: 1012 6417 BstAPI GCAn_nnn'nTGC Cuts at: 794 3259 3708 794 Size: 2465 449 4515 Fragments arranged by size: 4515 2465 449 BstBI TT'CG_AA Cuts at: 6233 6233 Size: 7429 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7429 BstXI CCAn_nnnn'nTGG Cuts at: 2128 4127 2128 Size: 1999 5430 BstYI r'GATC_y Cuts at: 1972 2073 2413 2715 2939 3268 4153 4198 4759 Size: 101 340 302 224 329 885 45 561 Cuts at: 4759 5126 5511 5528 6980 7066 7078 7424 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 885 561 385 367 346 340 329 302 224 101 86 45 17 12 BstZ17I GTA'TAC Cuts at: 4368 4368 Size: 7429 BtgI C'CryG_G Cuts at: 513 1065 2004 2619 3237 513 Size: 552 939 615 618 4705 Fragments arranged by size: 4705 939 618 615 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3775 487 Size: 683 56 2549 4141 Fragments arranged by size: 4141 2549 683 56 BtsI GCAGTG_nn' Cuts at: 2841 3370 3518 4945 5739 5759 2841 Size: 529 148 1427 794 20 4511 Fragments arranged by size: 4511 1427 794 529 148 20 ClaI AT'CG_AT Cuts at: 1554 3031 1554 Size: 1477 5952 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3784 3819 529 Size: 35 3220 35 4139 Fragments arranged by size: 4139 3220 35 35 DraI TTT'AAA Cuts at: 4733 4733 Size: 7429 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6436 809 Size: 507 5120 1802 Fragments arranged by size: 5120 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3469 4876 5765 Size: 54 1425 50 275 18 1639 1407 889 Cuts at: 5765 7394 8 Size: 1629 43 Fragments arranged by size: 1639 1629 1425 1407 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4876 1537 Size: 275 3064 4090 Fragments arranged by size: 4090 3064 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2162 5359 1353 Size: 809 3197 3423 Fragments arranged by size: 3423 3197 809 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2583 6016 6390 6536 387 Size: 1064 138 994 3433 374 146 1280 Fragments arranged by size: 3433 1280 1064 994 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2252 2802 3185 3837 4087 4467 Size: 393 593 27 550 383 652 250 380 Cuts at: 4467 5492 6089 6876 7144 1239 Size: 1025 597 787 268 1524 Fragments arranged by size: 1524 1025 787 652 597 593 550 393 383 380 268 250 27 EcoO109I rG'GnC_Cy Cuts at: 2269 3598 2269 Size: 1329 6100 EcoRI G'AATT_C Cuts at: 3086 4738 5116 3086 Size: 1652 378 5399 Fragments arranged by size: 5399 1652 378 EcoRV GAT'ATC Cuts at: 3421 3421 Size: 7429 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3347 4585 3347 Size: 1238 6191 FspI TGC'GCA Cuts at: 5935 5935 Size: 7429 HaeII r_GCGC'y Cuts at: 1678 4064 6576 1678 Size: 2386 2512 2531 Fragments arranged by size: 2531 2512 2386 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2306 2338 2564 2596 3641 Size: 32 135 32 734 32 226 32 1045 Cuts at: 3641 3673 4122 4154 4183 4215 4257 4289 6070 Size: 32 449 32 29 32 42 32 1781 Cuts at: 6070 6102 6144 6176 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1781 1045 734 449 226 135 42 42 32 32 32 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 669 1657 1938 4773 5019 669 Size: 988 281 2835 246 3079 Fragments arranged by size: 3079 2835 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7429 HpaI GTT'AAC Cuts at: 5019 5019 Size: 7429 KpnI G_GTAC'C Cuts at: 4754 4754 Size: 7429 MfeI C'AATT_G Cuts at: 2250 3015 5028 2250 Size: 765 2013 4651 Fragments arranged by size: 4651 2013 765 MluI A'CGCG_T Cuts at: 7305 7305 Size: 7429 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2310 3979 6543 6727 1305 Size: 1005 1669 2564 184 2007 Fragments arranged by size: 2564 2007 1669 1005 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5607 Fragments arranged by size: 5607 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2084 2126 2644 3803 5387 5746 5905 Size: 648 918 42 518 1159 1584 359 159 Cuts at: 5905 518 Size: 2042 Fragments arranged by size: 2042 1584 1159 918 648 518 359 159 42 MspA1I CmG'CkG Cuts at: 1606 4090 4189 4589 4845 5522 6670 6915 1606 Size: 2484 99 400 256 677 1148 245 2120 Fragments arranged by size: 2484 2120 1148 677 400 256 245 99 NcoI C'CATG_G Cuts at: 513 1065 2004 2619 3237 513 Size: 552 939 615 618 4705 Fragments arranged by size: 4705 939 618 615 552 NdeI CA'TA_TG Cuts at: 387 3944 387 Size: 3557 3872 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7429 NotI GC'GGCC_GC Cuts at: 4876 4876 Size: 7429 NsiI A_TGCA'T Cuts at: 2536 2536 Size: 7429 NspI r_CATG'y Cuts at: 1132 1229 3511 3982 4501 4787 6332 1132 Size: 97 2282 471 519 286 1545 2229 Fragments arranged by size: 2282 2229 1545 519 471 286 97 PasI CC'CwG_GG Cuts at: 1348 3750 1348 Size: 2402 5027 PciI A'CATG_T Cuts at: 6328 6328 Size: 7429 PflMI CCAn_nnn'nTGG Cuts at: 1108 2620 2780 1108 Size: 1512 160 5757 Fragments arranged by size: 5757 1512 160 PfoI T'CCnGG_A Cuts at: 3406 3406 Size: 7429 PmeI GTTT'AAAC Cuts at: 4733 4733 Size: 7429 PmlI CAC'GTG Cuts at: 2319 2768 2319 Size: 449 6980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5498 5530 7011 7043 5498 Size: 32 1481 32 5884 Fragments arranged by size: 5884 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2269 3598 2269 Size: 1329 6100 PshAI GACnn'nnGTC Cuts at: 1613 3818 1613 Size: 2205 5224 PsiI TTA'TAA Cuts at: 4526 4999 4526 Size: 473 6956 PspXI vC'TCGA_Gb Cuts at: 2829 2829 Size: 7429 PstI C_TGCA'G Cuts at: 830 2467 3554 4781 830 Size: 1637 1087 1227 3478 Fragments arranged by size: 3478 1637 1227 1087 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7429 PvuII CAG'CTG Cuts at: 4090 4589 4090 Size: 499 6930 SalI G'TCGA_C Cuts at: 1936 4771 1936 Size: 2835 4594 SanDI GG'GwC_CC Cuts at: 3598 3598 Size: 7429 SbfI CC_TGCA'GG Cuts at: 4781 4781 Size: 7429 ScaI AGT'ACT Cuts at: 5677 7389 5677 Size: 1712 5717 SexAI A'CCwGG_T Cuts at: 2564 3642 2564 Size: 1078 6351 SfcI C'TryA_G Cuts at: 826 1045 2463 3550 4692 4777 5912 6593 6784 Size: 219 1418 1087 1142 85 1135 681 191 Cuts at: 6784 826 Size: 1471 Fragments arranged by size: 1471 1418 1142 1135 1087 681 219 191 85 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7429 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7429 SmaI CCC'GGG Cuts at: 1872 4756 1872 Size: 2884 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2583 2719 2829 4223 5533 6434 Size: 197 926 640 136 110 1394 1310 901 Cuts at: 6434 6696 820 Size: 262 1553 Fragments arranged by size: 1553 1394 1310 926 901 640 262 197 136 110 SnaBI TAC'GTA Cuts at: 493 5220 493 Size: 4727 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7429 SphI G_CATG'C Cuts at: 1132 4501 4787 1132 Size: 3369 286 3774 Fragments arranged by size: 3774 3369 286 SspI AAT'ATT Cuts at: 5 52 3780 4032 5353 5 Size: 47 3728 252 1321 2081 Fragments arranged by size: 3728 2081 1321 252 47 StuI AGG'CCT Cuts at: 1517 2705 1517 Size: 1188 6241 StyI C'CwwG_G Cuts at: 513 1065 2004 2619 3237 3967 4126 4512 4868 Size: 552 939 615 618 730 159 386 356 Cuts at: 4868 513 Size: 3074 Fragments arranged by size: 3074 939 730 618 615 552 386 356 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2288 2434 2726 5456 5473 5626 5811 Size: 1255 708 146 292 2730 17 153 185 Cuts at: 5811 6256 325 Size: 445 1498 Fragments arranged by size: 2730 1498 1255 708 445 292 185 153 146 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3456 4292 4437 Size: 274 80 33 51 1443 1479 836 145 Cuts at: 4437 5675 7387 96 Size: 1238 1712 138 Fragments arranged by size: 1712 1479 1443 1238 836 274 145 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2384 2416 2671 2703 2745 2777 7389 7421 2384 Size: 32 255 32 42 32 4612 32 2392 Fragments arranged by size: 4612 2392 255 42 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7429 XbaI T'CTAG_A Cuts at: 4765 4765 Size: 7429 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2293 3730 2293 Size: 1437 5992 XhoI C'TCGA_G Cuts at: 1943 2583 2719 2829 1943 Size: 640 136 110 6543 Fragments arranged by size: 6543 640 136 110 XmaI C'CCGG_G Cuts at: 1870 4754 1870 Size: 2884 4545 XmnI GAAnn'nnTTC Cuts at: 5558 5558 Size: 7429 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5237 276 Size: 53 83 186 936 3703 2468 Fragments arranged by size: 3703 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI