(Circular) (Six-base) MAPSORT of: pFN21AB6440.seq Check: 7631 from: 1 to: 5740 pFN21AB6440 5740 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3550 278 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3083 1937 Size: 1146 4594 Acc65I G'GTAC_C Cuts at: 3061 3061 Size: 5740 AclI AA'CG_TT Cuts at: 1563 3867 4240 1563 Size: 2304 373 3063 Fragments arranged by size: 3063 2304 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3803 5187 5455 1632 Size: 2171 1384 268 1917 Fragments arranged by size: 2171 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5543 AflIII A'CryG_T Cuts at: 1203 4639 5616 1203 Size: 3436 977 1327 Fragments arranged by size: 3436 1327 977 AgeI A'CCGG_T Cuts at: 4571 4571 Size: 5740 AhdI GACnn_n'nnGTC Cuts at: 4469 4469 Size: 5740 AleI CACnn'nnGTG Cuts at: 1166 2822 1166 Size: 1656 4084 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5740 AlwNI CAG_nnn'CTG Cuts at: 3483 5055 3483 Size: 1572 4168 ApaLI G'TGCA_C Cuts at: 2374 3797 4953 2374 Size: 1423 1156 3161 Fragments arranged by size: 3161 1423 1156 ApoI r'AATT_y Cuts at: 1493 2858 3049 3275 3427 5536 1493 Size: 1365 191 226 152 2109 1697 Fragments arranged by size: 2109 1697 1365 226 191 152 AseI AT'TA_AT Cuts at: 160 1032 4294 160 Size: 872 3262 1606 Fragments arranged by size: 3262 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 AvaI C'yCGr_G Cuts at: 1870 1943 2032 2175 3065 1870 Size: 73 89 143 890 4545 Fragments arranged by size: 4545 890 143 89 73 BamHI G'GATC_C Cuts at: 3070 3437 3070 Size: 367 5373 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3061 4516 618 Size: 325 217 627 1274 1455 1842 Fragments arranged by size: 1842 1455 1274 627 325 217 BanII G_rGCy'C Cuts at: 1391 2399 3059 1391 Size: 1008 660 4072 Fragments arranged by size: 4072 1008 660 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4769 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3931 3965 1903 Size: 34 1994 34 3678 Fragments arranged by size: 3678 1994 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3632 4842 3632 Size: 1210 4530 BclI T'GATC_A Cuts at: 1548 1548 Size: 5740 BfrBI ATG'CAT Cuts at: 3020 3020 Size: 5740 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4351 136 Size: 107 122 71 631 605 2679 1525 Fragments arranged by size: 2679 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5735 5735 Size: 5740 BlpI GC'TnA_GC Cuts at: 3157 3157 Size: 5740 Bme1580I G_kGCm'C Cuts at: 1790 2378 3801 4957 1790 Size: 588 1423 1156 2573 Fragments arranged by size: 2573 1423 1156 588 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5740 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2349 2765 3471 Size: 416 53 83 399 24 932 416 706 Cuts at: 3471 4429 442 Size: 958 1753 Fragments arranged by size: 1753 958 932 706 416 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5740 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1998 4400 1239 Size: 759 2402 2579 Fragments arranged by size: 2579 2402 759 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3865 4730 5028 3865 Size: 865 298 4577 Fragments arranged by size: 4577 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4403 882 Size: 617 2904 2219 Fragments arranged by size: 2904 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2148 3531 493 Size: 711 944 1383 2702 Fragments arranged by size: 2702 1383 944 711 BsaBI GATnn'nnATC Cuts at: 5734 5734 Size: 5740 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3547 3929 4564 275 Size: 53 83 186 936 2014 382 635 1451 Fragments arranged by size: 2014 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4173 4571 4845 4992 5403 4173 Size: 398 274 147 411 4510 Fragments arranged by size: 4510 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2826 2856 1178 Size: 30 165 30 1423 30 4062 Fragments arranged by size: 4062 1423 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2193 1186 Size: 1007 4733 BseYI C'CCAG_C Cuts at: 4943 5439 4943 Size: 496 5244 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2551 1163 Size: 573 815 4352 Fragments arranged by size: 4352 815 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3190 3951 4979 1540 Size: 275 186 1189 761 1028 2301 Fragments arranged by size: 2301 1189 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2022 2378 2419 3801 3886 4957 2022 Size: 356 41 1382 85 1071 2805 Fragments arranged by size: 2805 1382 1071 356 85 41 BsiWI C'GTAC_G Cuts at: 2287 2287 Size: 5740 BsmI GAATG_Cn' Cuts at: 2606 2877 3251 3344 2606 Size: 271 374 93 5002 Fragments arranged by size: 5002 374 271 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2378 2399 2419 3059 3801 3886 Size: 399 232 356 21 20 640 742 85 Cuts at: 3886 4957 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 640 399 356 232 85 21 20 BspEI T'CCGG_A Cuts at: 5403 5403 Size: 5740 BspHI T'CATG_A Cuts at: 3629 5359 3629 Size: 1730 4010 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2573 3095 844 Size: 1729 522 3489 Fragments arranged by size: 3489 1729 522 BsrBI CCG'CTC Cuts at: 1394 1823 3627 1394 Size: 429 1804 3507 Fragments arranged by size: 3507 1804 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4235 4409 65 Size: 1183 2987 174 1396 Fragments arranged by size: 2987 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4384 4571 1700 Size: 110 2574 187 2869 Fragments arranged by size: 2869 2574 187 110 BsrGI T'GTAC_A Cuts at: 96 2240 96 Size: 2144 3596 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5740 BssSI C'ACGA_G Cuts at: 3800 4812 3800 Size: 1012 4728 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5740 BstBI TT'CG_AA Cuts at: 4544 4544 Size: 5740 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5740 BstYI r'GATC_y Cuts at: 1972 3070 3437 3822 3839 5291 5377 5389 5735 Size: 1098 367 385 17 1452 86 12 346 Cuts at: 5735 1972 Size: 1977 Fragments arranged by size: 1977 1452 1098 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5188 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2555 487 Size: 683 56 1329 3672 Fragments arranged by size: 3672 1329 683 56 BtsI GCAGTG_nn' Cuts at: 3256 4050 4070 3256 Size: 794 20 4926 Fragments arranged by size: 4926 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5740 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5705 DraI TTT'AAA Cuts at: 3044 3044 Size: 5740 DraIII CAC_nnn'GTG Cuts at: 2465 2465 Size: 5740 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2070 4747 809 Size: 507 754 2677 1802 Fragments arranged by size: 2677 1802 754 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3187 4076 5705 Size: 54 1425 50 275 18 1357 889 1629 Cuts at: 5705 8 Size: 43 Fragments arranged by size: 1629 1425 1357 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3187 1537 Size: 275 1375 4090 Fragments arranged by size: 4090 1375 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2133 3670 1353 Size: 780 1537 3423 Fragments arranged by size: 3423 1537 780 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4327 4701 4847 387 Size: 1064 138 2738 374 146 1280 Fragments arranged by size: 2738 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1998 3803 4400 5187 5455 1239 Size: 393 366 1805 597 787 268 1524 Fragments arranged by size: 1805 1524 787 597 393 366 268 EcoNI CCTnn'n_nnAGG Cuts at: 2208 2208 Size: 5740 EcoRI G'AATT_C Cuts at: 3049 3427 3049 Size: 378 5362 FspI TGC'GCA Cuts at: 4246 4246 Size: 5740 HaeII r_GCGC'y Cuts at: 1678 2361 4887 1678 Size: 683 2526 2531 Fragments arranged by size: 2531 2526 683 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4381 4413 4455 4487 1373 Size: 32 135 32 2809 32 42 32 2626 Fragments arranged by size: 2809 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3084 3330 669 Size: 988 281 1146 246 3079 Fragments arranged by size: 3079 1146 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5740 HpaI GTT'AAC Cuts at: 3330 3330 Size: 5740 KpnI G_GTAC'C Cuts at: 3065 3065 Size: 5740 MfeI C'AATT_G Cuts at: 3339 3339 Size: 5740 MluI A'CGCG_T Cuts at: 5616 5616 Size: 5740 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4854 5038 1305 Size: 3549 184 2007 Fragments arranged by size: 3549 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3918 Fragments arranged by size: 3918 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2822 3698 4057 4216 518 Size: 648 1656 876 359 159 2042 Fragments arranged by size: 2042 1656 876 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2483 3156 3833 4981 5226 1606 Size: 877 673 677 1148 245 2120 Fragments arranged by size: 2120 1148 877 677 673 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5188 NdeI CA'TA_TG Cuts at: 387 387 Size: 5740 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5740 NotI GC'GGCC_GC Cuts at: 3187 3187 Size: 5740 NsiI A_TGCA'T Cuts at: 3022 3022 Size: 5740 NspI r_CATG'y Cuts at: 1132 1229 2540 3098 4643 1132 Size: 97 1311 558 1545 2229 Fragments arranged by size: 2229 1545 1311 558 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5740 PciI A'CATG_T Cuts at: 4639 4639 Size: 5740 PflMI CCAn_nnn'nTGG Cuts at: 1108 2483 2642 1108 Size: 1375 159 4206 Fragments arranged by size: 4206 1375 159 PmeI GTTT'AAAC Cuts at: 3044 3044 Size: 5740 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3809 3841 5322 5354 3809 Size: 32 1481 32 4195 Fragments arranged by size: 4195 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5740 PsiI TTA'TAA Cuts at: 2472 3310 2472 Size: 838 4902 PstI C_TGCA'G Cuts at: 830 3092 830 Size: 2262 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5740 PvuII CAG'CTG Cuts at: 2483 2483 Size: 5740 SalI G'TCGA_C Cuts at: 1936 3082 1936 Size: 1146 4594 SbfI CC_TGCA'GG Cuts at: 3092 3092 Size: 5740 ScaI AGT'ACT Cuts at: 3988 5700 3988 Size: 1712 4028 SfcI C'TryA_G Cuts at: 826 1045 3088 4223 4904 5095 826 Size: 219 2043 1135 681 191 1471 Fragments arranged by size: 2043 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5740 SmaI CCC'GGG Cuts at: 1872 3067 1872 Size: 1195 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2032 3844 4745 5007 820 Size: 197 926 89 1812 901 262 1553 Fragments arranged by size: 1812 1553 926 901 262 197 89 SnaBI TAC'GTA Cuts at: 493 2148 3531 493 Size: 1655 1383 2702 Fragments arranged by size: 2702 1655 1383 SpeI A'CTAG_T Cuts at: 152 152 Size: 5740 SphI G_CATG'C Cuts at: 1132 3098 1132 Size: 1966 3774 SspI AAT'ATT Cuts at: 5 52 3664 5 Size: 47 3612 2081 Fragments arranged by size: 3612 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5740 StyI C'CwwG_G Cuts at: 513 1065 2830 3179 513 Size: 552 1765 349 3074 Fragments arranged by size: 3074 1765 552 349 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2260 3767 3784 3937 4122 4567 325 Size: 1255 680 1507 17 153 185 445 1498 Fragments arranged by size: 1507 1498 1255 680 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2240 3986 5698 Size: 274 80 33 51 1443 263 1746 1712 Cuts at: 5698 96 Size: 138 Fragments arranged by size: 1746 1712 1443 274 263 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2906 2930 2938 2962 5700 5732 2906 Size: 24 8 24 2738 32 2914 Fragments arranged by size: 2914 2738 32 24 24 8 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5740 XbaI T'CTAG_A Cuts at: 3076 3076 Size: 5740 XhoI C'TCGA_G Cuts at: 1943 2032 1943 Size: 89 5651 XmaI C'CCGG_G Cuts at: 1870 3065 1870 Size: 1195 4545 XmnI GAAnn'nnTTC Cuts at: 2491 3869 2491 Size: 1378 4362 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3548 276 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI