(Circular) (Six-base) MAPSORT of: pFN21AB8539.seq Check: 1905 from: 1 to: 7570 pFN21AB8539 7570 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2595 4372 4390 4399 4741 2595 Size: 1777 18 9 342 5424 Fragments arranged by size: 5424 1777 342 18 9 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3153 3429 3786 4059 Size: 53 83 186 936 1617 276 357 273 Cuts at: 4059 5380 278 Size: 1321 2468 Fragments arranged by size: 2468 1617 1321 936 357 276 273 186 83 53 AccI GT'mk_AC Cuts at: 1937 3959 4913 1937 Size: 2022 954 4594 Fragments arranged by size: 4594 2022 954 Acc65I G'GTAC_C Cuts at: 3237 3390 4891 3237 Size: 153 1501 5916 Fragments arranged by size: 5916 1501 153 AclI AA'CG_TT Cuts at: 1563 5697 6070 1563 Size: 4134 373 3063 Fragments arranged by size: 4134 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3302 4523 5633 7017 7285 1632 Size: 1670 1221 1110 1384 268 1917 Fragments arranged by size: 1917 1670 1384 1221 1110 268 AfeI AGC'GCT Cuts at: 4571 4571 Size: 7570 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7373 AflIII A'CryG_T Cuts at: 1203 6469 7446 1203 Size: 5266 977 1327 Fragments arranged by size: 5266 1327 977 AgeI A'CCGG_T Cuts at: 6401 6401 Size: 7570 AhdI GACnn_n'nnGTC Cuts at: 6299 6299 Size: 7570 AleI CACnn'nnGTG Cuts at: 1166 2607 4845 1166 Size: 1441 2238 3891 Fragments arranged by size: 3891 2238 1441 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7570 AlwNI CAG_nnn'CTG Cuts at: 3016 4127 4219 4255 5313 6885 3016 Size: 1111 92 36 1058 1572 3701 Fragments arranged by size: 3701 1572 1111 1058 92 36 ApaI G_GGCC'C Cuts at: 4095 4095 Size: 7570 ApaLI G'TGCA_C Cuts at: 3064 5627 6783 3064 Size: 2563 1156 3851 Fragments arranged by size: 3851 2563 1156 ApoI r'AATT_y Cuts at: 1493 4306 4879 5105 5257 7366 1493 Size: 2813 573 226 152 2109 1697 Fragments arranged by size: 2813 2109 1697 573 226 152 AseI AT'TA_AT Cuts at: 160 1032 6124 160 Size: 872 5092 1606 Fragments arranged by size: 5092 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7570 AvaI C'yCGr_G Cuts at: 1870 1943 3662 3821 4100 4330 4895 1870 Size: 73 1719 159 279 230 565 4545 Fragments arranged by size: 4545 1719 565 279 230 159 73 BamHI G'GATC_C Cuts at: 4900 5267 4900 Size: 367 7203 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2551 3237 3390 4481 4891 Size: 325 217 627 764 686 153 1091 410 Cuts at: 4891 6346 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1091 764 686 627 410 325 217 153 BanII G_rGCy'C Cuts at: 1391 2043 2886 2976 3942 4095 4779 4830 4889 Size: 652 843 90 966 153 684 51 59 Cuts at: 4889 1391 Size: 4072 Fragments arranged by size: 4072 966 843 684 652 153 90 59 51 BbeI G_GCGC'C Cuts at: 4485 4485 Size: 7570 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4051 928 Size: 971 2152 4447 Fragments arranged by size: 4447 2152 971 BbvCI CC'TCA_GC Cuts at: 3761 4115 3761 Size: 354 7216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2426 2460 5761 5795 1903 Size: 34 50 34 405 34 3301 34 3678 Fragments arranged by size: 3678 3301 405 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5462 6672 5462 Size: 1210 6360 BclI T'GATC_A Cuts at: 1548 1548 Size: 7570 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3296 3617 Size: 107 122 71 631 605 337 1287 321 Cuts at: 3617 4490 6181 136 Size: 873 1691 1525 Fragments arranged by size: 1691 1525 1287 873 631 605 337 321 122 107 71 BglII A'GATC_T Cuts at: 7565 7565 Size: 7570 BlpI GC'TnA_GC Cuts at: 4583 4987 4583 Size: 404 7166 Bme1580I G_kGCm'C Cuts at: 1790 2232 2556 3068 4095 5631 6787 1790 Size: 442 324 512 1027 1536 1156 2573 Fragments arranged by size: 2573 1536 1156 1027 512 442 324 BmgBI CAC'GTC Cuts at: 1318 2676 3724 1318 Size: 1358 1048 5164 Fragments arranged by size: 5164 1358 1048 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3557 4505 5301 Size: 416 53 83 399 24 2140 948 796 Cuts at: 5301 6259 442 Size: 958 1753 Fragments arranged by size: 2140 1753 958 948 796 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7570 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4608 4849 4608 Size: 241 7329 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2720 2799 2876 3074 3263 3449 3538 3821 Size: 1481 79 77 198 189 186 89 283 Cuts at: 3821 4325 4793 6230 1239 Size: 504 468 1437 2579 Fragments arranged by size: 2579 1481 1437 504 468 283 198 189 186 89 79 77 Bpu10I CC'TnA_GC Cuts at: 2702 3761 4115 2702 Size: 1059 354 6157 Fragments arranged by size: 6157 1059 354 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3119 5695 6560 6858 3119 Size: 2576 865 298 3831 Fragments arranged by size: 3831 2576 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2933 3023 3659 4329 6233 882 Size: 617 1434 90 636 670 1904 2219 Fragments arranged by size: 2219 1904 1434 670 636 617 90 BsaAI yAC'GTr Cuts at: 493 1204 3331 3373 5361 493 Size: 711 2127 42 1988 2702 Fragments arranged by size: 2702 2127 1988 711 42 BsaBI GATnn'nnATC Cuts at: 7564 7564 Size: 7570 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3150 3426 3687 3783 Size: 53 83 186 936 1617 276 261 96 Cuts at: 3783 3962 4056 4445 4482 5377 5759 6394 275 Size: 179 94 389 37 895 382 635 1451 Fragments arranged by size: 1617 1451 936 895 635 389 382 276 261 186 179 96 94 83 53 37 BsaWI w'CCGG_w Cuts at: 2064 3198 4150 4470 6003 6401 6675 6822 7233 Size: 1134 952 320 1533 398 274 147 411 Cuts at: 7233 2064 Size: 2401 Fragments arranged by size: 2401 1533 1134 952 411 398 320 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2111 2141 3047 3077 3374 Size: 30 165 30 708 30 906 30 297 Cuts at: 3374 3404 3536 3566 1178 Size: 30 132 30 5182 Fragments arranged by size: 5182 906 708 297 165 132 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2707 3170 3284 3395 3818 4118 1186 Size: 1521 463 114 111 423 300 4638 Fragments arranged by size: 4638 1521 463 423 300 114 111 BseYI C'CCAG_C Cuts at: 2761 3004 3539 3705 3908 3947 4077 4082 6773 Size: 243 535 166 203 39 130 5 2691 Cuts at: 6773 7269 2761 Size: 496 3062 Fragments arranged by size: 3062 2691 535 496 243 203 166 130 39 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2384 2924 3587 3622 4316 4379 4424 Size: 573 648 540 663 35 694 63 45 Cuts at: 4424 1163 Size: 4309 Fragments arranged by size: 4309 694 663 648 573 540 63 45 35 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 2622 4074 5020 5781 6809 Size: 275 186 12 609 1452 946 761 1028 Cuts at: 6809 1540 Size: 2301 Fragments arranged by size: 2301 1452 1028 946 761 609 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2868 3068 4416 5631 5716 6787 2868 Size: 200 1348 1215 85 1071 3651 Fragments arranged by size: 3651 1348 1215 1071 200 85 BsmI GAATG_Cn' Cuts at: 2908 5081 5174 2908 Size: 2173 93 5304 Fragments arranged by size: 5304 2173 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2686 2719 3239 4837 2686 Size: 33 520 1598 5419 Fragments arranged by size: 5419 1598 520 33 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2232 2556 2868 2886 2976 3068 Size: 399 253 189 324 312 18 90 92 Cuts at: 3068 3942 4095 4416 4779 4830 4889 5631 5716 Size: 874 153 321 363 51 59 742 85 Cuts at: 5716 6787 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 874 742 399 363 324 321 312 253 189 153 92 90 85 59 51 18 BspEI T'CCGG_A Cuts at: 2064 4150 7233 2064 Size: 2086 3083 2401 Fragments arranged by size: 3083 2401 2086 BspHI T'CATG_A Cuts at: 5459 7189 5459 Size: 1730 5840 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2595 3045 4372 4390 4399 4741 4925 844 Size: 1751 450 1327 18 9 342 184 3489 Fragments arranged by size: 3489 1751 1327 450 342 184 18 9 BsrBI CCG'CTC Cuts at: 1394 1823 2127 3145 3552 5457 1394 Size: 429 304 1018 407 1905 3507 Fragments arranged by size: 3507 1905 1018 429 407 304 BsrDI GCAATG_nn' Cuts at: 65 1248 3211 3357 3721 6065 6239 65 Size: 1183 1963 146 364 2344 174 1396 Fragments arranged by size: 2344 1963 1396 1183 364 174 146 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 3698 3875 4800 6214 6401 1700 Size: 110 198 1690 177 925 1414 187 2869 Fragments arranged by size: 2869 1690 1414 925 198 187 177 110 BsrGI T'GTAC_A Cuts at: 96 2529 96 Size: 2433 5137 BssHII G'CGCG_C Cuts at: 1925 4395 1925 Size: 2470 5100 BssSI C'ACGA_G Cuts at: 3482 5630 6642 3482 Size: 2148 1012 4410 Fragments arranged by size: 4410 2148 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4411 794 Size: 3617 3953 BstBI TT'CG_AA Cuts at: 6374 6374 Size: 7570 BstEII G'GTnAC_C Cuts at: 1184 2285 1184 Size: 1101 6469 BstXI CCAn_nnnn'nTGG Cuts at: 2769 2983 3799 4372 4546 4663 2769 Size: 214 816 573 174 117 5676 Fragments arranged by size: 5676 816 573 214 174 117 BstYI r'GATC_y Cuts at: 1972 4900 5267 5652 5669 7121 7207 7219 7565 Size: 2928 367 385 17 1452 86 12 346 Cuts at: 7565 1972 Size: 1977 Fragments arranged by size: 2928 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4128 4128 Size: 7570 BtgI C'CryG_G Cuts at: 513 1065 2556 3312 3744 3792 4632 513 Size: 552 1491 756 432 48 840 3451 Fragments arranged by size: 3451 1491 840 756 552 432 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2040 2392 487 Size: 683 56 814 352 5665 Fragments arranged by size: 5665 814 683 352 56 BtsI GCAGTG_nn' Cuts at: 2401 2807 5086 5880 5900 2401 Size: 406 2279 794 20 4071 Fragments arranged by size: 4071 2279 794 406 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7570 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7535 DraI TTT'AAA Cuts at: 4874 4874 Size: 7570 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6577 809 Size: 507 5261 1802 Fragments arranged by size: 5261 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2115 2209 Size: 54 1425 50 275 18 180 105 94 Cuts at: 2209 2364 2472 2619 3072 3288 3297 3609 3771 Size: 155 108 147 453 216 9 312 162 Cuts at: 3771 3789 3843 3895 4071 5017 5906 7535 8 Size: 18 54 52 176 946 889 1629 43 Fragments arranged by size: 1629 1425 946 889 453 312 275 216 180 176 162 155 147 108 105 94 54 54 52 50 43 18 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2010 2619 4071 5017 1537 Size: 275 198 609 1452 946 4090 Fragments arranged by size: 4090 1452 946 609 275 198 EarI CTCTTCn'nnn_ Cuts at: 1353 2939 3662 4277 5500 1353 Size: 1586 723 615 1223 3423 Fragments arranged by size: 3423 1586 1223 723 615 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2102 2321 3964 6157 6531 6677 Size: 1064 138 513 219 1643 2193 374 146 Cuts at: 6677 387 Size: 1280 Fragments arranged by size: 2193 1643 1280 1064 513 374 219 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2720 2799 2876 3074 3263 3302 3449 Size: 393 1088 79 77 198 189 39 147 Cuts at: 3449 3538 3821 4325 4523 4793 5633 6230 7017 Size: 89 283 504 198 270 840 597 787 Cuts at: 7017 7285 1239 Size: 268 1524 Fragments arranged by size: 1524 1088 840 787 597 504 393 283 270 268 198 198 189 147 89 79 77 39 EcoO109I rG'GnC_Cy Cuts at: 3469 3544 3904 4091 4124 4198 4492 3469 Size: 75 360 187 33 74 294 6547 Fragments arranged by size: 6547 360 294 187 75 74 33 EcoRI G'AATT_C Cuts at: 4879 5257 4879 Size: 378 7192 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3469 3469 Size: 7570 FspI TGC'GCA Cuts at: 3713 4814 6076 3713 Size: 1101 1262 5207 Fragments arranged by size: 5207 1262 1101 HaeII r_GCGC'y Cuts at: 1678 3464 3623 3885 4485 4573 6717 1678 Size: 1786 159 262 600 88 2144 2531 Fragments arranged by size: 2531 2144 1786 600 262 159 88 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3536 3568 3815 3847 6211 Size: 32 135 32 1964 32 247 32 2364 Cuts at: 6211 6243 6285 6317 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2364 1964 247 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3960 4914 5160 669 Size: 988 281 2022 954 246 3079 Fragments arranged by size: 3079 2022 988 954 281 246 HindIII A'AGCT_T Cuts at: 748 4658 748 Size: 3910 3660 HpaI GTT'AAC Cuts at: 5160 5160 Size: 7570 KasI G'GCGC_C Cuts at: 4481 4481 Size: 7570 KpnI G_GTAC'C Cuts at: 3241 3394 4895 3241 Size: 153 1501 5916 Fragments arranged by size: 5916 1501 153 MfeI C'AATT_G Cuts at: 5169 5169 Size: 7570 MluI A'CGCG_T Cuts at: 7446 7446 Size: 7570 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2082 2937 3204 4270 6684 6868 1305 Size: 777 855 267 1066 2414 184 2007 Fragments arranged by size: 2414 2007 1066 855 777 267 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 2211 2366 3074 3290 Size: 54 1425 343 285 94 155 708 216 Cuts at: 3290 3611 3773 3791 3845 10 Size: 321 162 18 54 3735 Fragments arranged by size: 3735 1425 708 343 321 285 216 162 155 94 54 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 2300 2384 2468 2607 2609 4544 4845 Size: 648 1134 84 84 139 2 1935 301 Cuts at: 4845 5528 5887 6046 518 Size: 683 359 159 2042 Fragments arranged by size: 2042 1935 1134 683 648 359 301 159 139 84 84 2 MspA1I CmG'CkG Cuts at: 1606 2450 3004 3166 3324 3709 4216 4351 4453 Size: 844 554 162 158 385 507 135 102 Cuts at: 4453 4582 4986 5663 6811 7056 1606 Size: 129 404 677 1148 245 2120 Fragments arranged by size: 2120 1148 844 677 554 507 404 385 245 162 158 135 129 102 NaeI GCC'GGC Cuts at: 2010 3700 3877 2010 Size: 1690 177 5703 Fragments arranged by size: 5703 1690 177 NarI GG'CG_CC Cuts at: 4482 4482 Size: 7570 NcoI C'CATG_G Cuts at: 513 1065 2556 4632 513 Size: 552 1491 2076 3451 Fragments arranged by size: 3451 2076 1491 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7570 NgoMIV G'CCGG_C Cuts at: 2008 3698 3875 2008 Size: 1690 177 5703 Fragments arranged by size: 5703 1690 177 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7570 NotI GC'GGCC_GC Cuts at: 5017 5017 Size: 7570 NspI r_CATG'y Cuts at: 1132 1229 3064 4267 4928 6473 1132 Size: 97 1835 1203 661 1545 2229 Fragments arranged by size: 2229 1835 1545 1203 661 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7570 PciI A'CATG_T Cuts at: 6469 6469 Size: 7570 PflMI CCAn_nnn'nTGG Cuts at: 1108 2605 4633 1108 Size: 1497 2028 4045 Fragments arranged by size: 4045 2028 1497 PfoI T'CCnGG_A Cuts at: 2736 4683 2736 Size: 1947 5623 PmeI GTTT'AAAC Cuts at: 4874 4874 Size: 7570 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2303 2335 5639 5671 7152 7184 2303 Size: 32 3304 32 1481 32 2689 Fragments arranged by size: 3304 2689 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3544 3904 4124 4198 3544 Size: 360 220 74 6916 Fragments arranged by size: 6916 360 220 74 PshAI GACnn'nnGTC Cuts at: 1613 2678 1613 Size: 1065 6505 PsiI TTA'TAA Cuts at: 5140 5140 Size: 7570 PspOMI G'GGCC_C Cuts at: 4091 4091 Size: 7570 PstI C_TGCA'G Cuts at: 830 2802 3021 3735 4033 4224 4752 4922 830 Size: 1972 219 714 298 191 528 170 3478 Fragments arranged by size: 3478 1972 714 528 298 219 191 170 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7570 PvuII CAG'CTG Cuts at: 3004 3324 3709 4216 4351 4582 3004 Size: 320 385 507 135 231 5992 Fragments arranged by size: 5992 507 385 320 231 135 SalI G'TCGA_C Cuts at: 1936 3958 4912 1936 Size: 2022 954 4594 Fragments arranged by size: 4594 2022 954 SanDI GG'GwC_CC Cuts at: 3544 3904 3544 Size: 360 7210 SbfI CC_TGCA'GG Cuts at: 4752 4922 4752 Size: 170 7400 ScaI AGT'ACT Cuts at: 5818 7530 5818 Size: 1712 5858 SexAI A'CCwGG_T Cuts at: 2547 2547 Size: 7570 SfcI C'TryA_G Cuts at: 826 1045 2161 2798 3017 3319 3401 3731 4029 Size: 219 1116 637 219 302 82 330 298 Cuts at: 4029 4220 4748 4918 6053 6734 6925 826 Size: 191 528 170 1135 681 191 1471 Fragments arranged by size: 1471 1135 1116 681 637 528 330 302 298 219 219 191 191 170 82 SfiI GGCCn_nnn'nGGCC Cuts at: 3296 3296 Size: 7570 SfoI GGC'GCC Cuts at: 4483 4483 Size: 7570 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7570 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7570 SmaI CCC'GGG Cuts at: 1872 4332 4897 1872 Size: 2460 565 4545 Fragments arranged by size: 4545 2460 565 SmlI C'TyrA_G Cuts at: 820 1017 1943 3134 5674 6575 6837 820 Size: 197 926 1191 2540 901 262 1553 Fragments arranged by size: 2540 1553 1191 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5361 493 Size: 4868 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7570 SphI G_CATG'C Cuts at: 1132 4928 1132 Size: 3796 3774 SspI AAT'ATT Cuts at: 5 52 5494 5 Size: 47 5442 2081 Fragments arranged by size: 5442 2081 47 StuI AGG'CCT Cuts at: 1517 2441 2537 4109 1517 Size: 924 96 1572 4978 Fragments arranged by size: 4978 1572 924 96 StyI C'CwwG_G Cuts at: 513 1065 2019 2151 2556 3514 3669 4632 5009 Size: 552 954 132 405 958 155 963 377 Cuts at: 5009 513 Size: 3074 Fragments arranged by size: 3074 963 958 954 552 405 377 155 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2597 4330 5597 5614 5767 5952 6397 Size: 1255 1017 1733 1267 17 153 185 445 Cuts at: 6397 325 Size: 1498 Fragments arranged by size: 1733 1498 1267 1255 1017 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2529 5816 7528 Size: 274 80 33 51 1443 552 3287 1712 Cuts at: 7528 96 Size: 138 Fragments arranged by size: 3287 1712 1443 552 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2560 2592 3047 3079 7530 7562 2560 Size: 32 455 32 4451 32 2568 Fragments arranged by size: 4451 2568 455 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2492 3119 1316 Size: 1176 627 5767 Fragments arranged by size: 5767 1176 627 XbaI T'CTAG_A Cuts at: 4906 4906 Size: 7570 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4630 4630 Size: 7570 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7570 XmaI C'CCGG_G Cuts at: 1870 4330 4895 1870 Size: 2460 565 4545 Fragments arranged by size: 4545 2460 565 XmnI GAAnn'nnTTC Cuts at: 4613 5699 4613 Size: 1086 6484 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3151 3427 3784 4057 Size: 53 83 186 936 1617 276 357 273 Cuts at: 4057 5378 276 Size: 1321 2468 Fragments arranged by size: 2468 1617 1321 936 357 276 273 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SrfI SwaI