(Linear) (Six-base) MAPSORT of: KIBB8523.seq Check: 1478 from: 1 to: 2811 KIBB8523.orf With 182 enzymes: SgfI * October 7, 2009 14:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 776 2811 Size: 776 2035 AatII G_ACGT'C Cuts at: 0 2324 2811 Size: 2324 487 AccI GT'mk_AC Cuts at: 0 596 2623 2811 Size: 596 2027 188 Fragments arranged by size: 2027 596 188 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1970 2639 2811 Size: 1970 669 172 Fragments arranged by size: 1970 669 172 AfeI AGC'GCT Cuts at: 0 2755 2811 Size: 2755 56 AflIII A'CryG_T Cuts at: 0 719 1539 1564 1763 2219 2811 Size: 719 820 25 199 456 592 Fragments arranged by size: 820 719 592 456 199 25 AhdI GACnn_n'nnGTC Cuts at: 0 46 2811 Size: 46 2765 AleI CACnn'nnGTG Cuts at: 0 283 866 989 1124 1604 1739 1862 2272 Size: 283 583 123 135 480 135 123 410 Cuts at: 2272 2811 Size: 539 Fragments arranged by size: 583 539 480 410 283 135 135 123 123 AlwNI CAG_nnn'CTG Cuts at: 0 843 2811 Size: 843 1968 ApaI G_GGCC'C Cuts at: 0 462 2811 Size: 462 2349 ApaLI G'TGCA_C Cuts at: 0 708 1074 1217 1340 2811 Size: 708 366 143 123 1471 Fragments arranged by size: 1471 708 366 143 123 ApoI r'AATT_y Cuts at: 0 1878 2007 2811 Size: 1878 129 804 Fragments arranged by size: 1878 804 129 AvaI C'yCGr_G Cuts at: 0 281 1448 2075 2811 Size: 281 1167 627 736 Fragments arranged by size: 1167 736 627 281 BamHI G'GATC_C Cuts at: 0 1984 2811 Size: 1984 827 BanI G'GyrC_C Cuts at: 0 397 2274 2811 Size: 397 1877 537 Fragments arranged by size: 1877 537 397 BanII G_rGCy'C Cuts at: 0 462 632 898 2194 2313 2426 2811 Size: 462 170 266 1296 119 113 385 Fragments arranged by size: 1296 462 385 266 170 119 113 BbsI GAAGACnn'nnnn_ Cuts at: 0 639 1567 1935 2522 2535 2612 2811 Size: 639 928 368 587 13 77 199 Fragments arranged by size: 928 639 587 368 199 77 13 BbvCI CC'TCA_GC Cuts at: 0 935 1796 1919 2811 Size: 935 861 123 892 Fragments arranged by size: 935 892 861 123 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 523 557 2811 Size: 523 34 2254 Fragments arranged by size: 2254 523 34 BglI GCCn_nnn'nGGC Cuts at: 0 538 1166 1289 2811 Size: 538 628 123 1522 Fragments arranged by size: 1522 628 538 123 Bme1580I G_kGCm'C Cuts at: 0 400 462 712 750 1078 1221 1344 2279 Size: 400 62 250 38 328 143 123 935 Cuts at: 2279 2811 Size: 532 Fragments arranged by size: 935 532 400 328 250 143 123 62 38 BmgBI CAC'GTC Cuts at: 0 1565 2811 Size: 1565 1246 BmrI ACTGGGnnnn_n' Cuts at: 0 416 2210 2811 Size: 416 1794 601 Fragments arranged by size: 1794 601 416 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2433 2811 Size: 2433 378 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 160 2074 2525 2811 Size: 160 1914 451 286 Fragments arranged by size: 1914 451 286 160 Bpu10I CC'TnA_GC Cuts at: 0 545 935 1796 1919 2312 2811 Size: 545 390 861 123 393 499 Fragments arranged by size: 861 545 499 393 390 123 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 316 1420 2473 2811 Size: 316 1104 1053 338 Fragments arranged by size: 1104 1053 338 316 BsaI GGTCTCn'nnnn_ Cuts at: 0 507 2811 Size: 507 2304 BsaAI yAC'GTr Cuts at: 0 82 1542 1766 2811 Size: 82 1460 224 1045 Fragments arranged by size: 1460 1045 224 82 BsaHI Gr'CG_yC Cuts at: 0 2321 2811 Size: 2321 490 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1529 1559 2811 Size: 1529 30 1252 Fragments arranged by size: 1529 1252 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 43 1276 2410 2811 Size: 43 1233 1134 401 Fragments arranged by size: 1233 1134 401 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 744 2811 Size: 744 2067 BsiHKAI G_wGCw'C Cuts at: 0 712 1078 1221 1344 1408 1547 1590 2811 Size: 712 366 143 123 64 139 43 1221 Fragments arranged by size: 1221 712 366 143 139 123 64 43 BsmI GAATG_Cn' Cuts at: 0 439 2811 Size: 439 2372 BsmBI CGTCTCn'nnnn_ Cuts at: 0 670 2328 2811 Size: 670 1658 483 Fragments arranged by size: 1658 670 483 Bsp1286I G_dGCh'C Cuts at: 0 400 462 632 712 750 898 1078 1221 Size: 400 62 170 80 38 148 180 143 Cuts at: 1221 1344 1408 1547 1590 2194 2279 2313 2426 Size: 123 64 139 43 604 85 34 113 Cuts at: 2426 2811 Size: 385 Fragments arranged by size: 604 400 385 180 170 148 143 139 123 113 85 80 64 62 43 38 34 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 776 1204 1327 1450 1696 2811 Size: 776 428 123 123 246 1115 Fragments arranged by size: 1115 776 428 246 123 123 BsrBI CCG'CTC Cuts at: 0 75 2074 2811 Size: 75 1999 737 Fragments arranged by size: 1999 737 75 BssSI C'ACGA_G Cuts at: 0 1120 2811 Size: 1120 1691 BstAPI GCAn_nnn'nTGC Cuts at: 0 1319 1943 2811 Size: 1319 624 868 Fragments arranged by size: 1319 868 624 BstXI CCAn_nnnn'nTGG Cuts at: 0 585 2811 Size: 585 2226 BstYI r'GATC_y Cuts at: 0 840 1510 1984 2811 Size: 840 670 474 827 Fragments arranged by size: 840 827 670 474 Bsu36I CC'TnA_GG Cuts at: 0 469 518 1532 2811 Size: 469 49 1014 1279 Fragments arranged by size: 1279 1014 469 49 BtgI C'CryG_G Cuts at: 0 392 484 527 851 984 1287 1353 1476 Size: 392 92 43 324 133 303 66 123 Cuts at: 1476 1599 1722 1845 2162 2811 Size: 123 123 123 317 649 Fragments arranged by size: 649 392 324 317 303 133 123 123 123 123 92 66 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 420 1568 2811 Size: 420 1148 1243 Fragments arranged by size: 1243 1148 420 BtsI GCAGTG_nn' Cuts at: 0 204 1155 1632 2811 Size: 204 951 477 1179 Fragments arranged by size: 1179 951 477 204 ClaI AT'CG_AT Cuts at: 0 1979 2811 Size: 1979 832 EaeI y'GGCC_r Cuts at: 0 413 530 656 2811 Size: 413 117 126 2155 Fragments arranged by size: 2155 413 126 117 EarI CTCTTCn'nnn_ Cuts at: 0 940 1997 2446 2811 Size: 940 1057 449 365 Fragments arranged by size: 1057 940 449 365 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 160 1970 2074 2525 2639 2811 Size: 160 1810 104 451 114 172 Fragments arranged by size: 1810 451 172 160 114 104 EcoNI CCTnn'n_nnAGG Cuts at: 0 342 934 1200 1692 2811 Size: 342 592 266 492 1119 Fragments arranged by size: 1119 592 492 342 266 EcoO109I rG'GnC_Cy Cuts at: 0 458 1431 2258 2811 Size: 458 973 827 553 Fragments arranged by size: 973 827 553 458 EcoRI G'AATT_C Cuts at: 0 1878 2811 Size: 1878 933 EcoRV GAT'ATC Cuts at: 0 2593 2811 Size: 2593 218 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2457 2629 2811 Size: 2457 172 182 Fragments arranged by size: 2457 182 172 HaeII r_GCGC'y Cuts at: 0 2757 2811 Size: 2757 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 32 64 369 401 1529 1561 1775 1807 Size: 32 32 305 32 1128 32 214 32 Cuts at: 1807 2308 2340 2811 Size: 501 32 471 Fragments arranged by size: 1128 501 471 305 214 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 204 1131 1254 1500 2811 Size: 204 927 123 246 1311 Fragments arranged by size: 1311 927 246 204 123 HindIII A'AGCT_T Cuts at: 0 2632 2811 Size: 2632 179 HpaI GTT'AAC Cuts at: 0 1131 2811 Size: 1131 1680 MfeI C'AATT_G Cuts at: 0 1958 2811 Size: 1958 853 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2592 2811 Size: 2592 219 MscI TGG'CCA Cuts at: 0 415 658 2811 Size: 415 243 2153 Fragments arranged by size: 2153 415 243 MslI CAynn'nnrTG Cuts at: 0 215 283 583 743 866 989 1001 1124 Size: 215 68 300 160 123 123 12 123 Cuts at: 1124 1358 1481 1604 1616 1727 1739 1741 1764 Size: 234 123 123 12 111 12 2 23 Cuts at: 1764 1862 2272 2811 Size: 98 410 539 Fragments arranged by size: 539 410 300 234 215 160 123 123 123 123 123 111 98 68 23 12 12 12 2 MspA1I CmG'CkG Cuts at: 0 1280 1368 2811 Size: 1280 88 1443 Fragments arranged by size: 1443 1280 88 NcoI C'CATG_G Cuts at: 0 851 1353 1476 1722 1845 2811 Size: 851 502 123 246 123 966 Fragments arranged by size: 966 851 502 246 123 123 NspI r_CATG'y Cuts at: 0 723 2223 2811 Size: 723 1500 588 Fragments arranged by size: 1500 723 588 PciI A'CATG_T Cuts at: 0 719 2219 2811 Size: 719 1500 592 Fragments arranged by size: 1500 719 592 PfoI T'CCnGG_A Cuts at: 0 836 2811 Size: 836 1975 PmlI CAC'GTG Cuts at: 0 82 1542 1766 2811 Size: 82 1460 224 1045 Fragments arranged by size: 1460 1045 224 82 PpuMI rG'GwC_Cy Cuts at: 0 1431 2811 Size: 1431 1380 PshAI GACnn'nnGTC Cuts at: 0 266 2811 Size: 266 2545 PsiI TTA'TAA Cuts at: 0 2707 2811 Size: 2707 104 PspOMI G'GGCC_C Cuts at: 0 458 2811 Size: 458 2353 PspXI vC'TCGA_Gb Cuts at: 0 2075 2811 Size: 2075 736 PstI C_TGCA'G Cuts at: 0 787 1201 1324 1693 2811 Size: 787 414 123 369 1118 Fragments arranged by size: 1118 787 414 369 123 PvuII CAG'CTG Cuts at: 0 1368 2811 Size: 1368 1443 SexAI A'CCwGG_T Cuts at: 0 170 1964 2811 Size: 170 1794 847 Fragments arranged by size: 1794 847 170 SfcI C'TryA_G Cuts at: 0 783 915 1197 1320 1689 1715 2811 Size: 783 132 282 123 369 26 1096 Fragments arranged by size: 1096 783 369 282 132 123 26 SmaI CCC'GGG Cuts at: 0 1450 2811 Size: 1450 1361 SmlI C'TyrA_G Cuts at: 0 331 1435 2075 2488 2811 Size: 331 1104 640 413 323 Fragments arranged by size: 1104 640 413 331 323 StyI C'CwwG_G Cuts at: 0 851 1353 1476 1722 1845 2241 2345 2811 Size: 851 502 123 246 123 396 104 466 Fragments arranged by size: 851 502 466 396 246 123 123 104 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 223 2811 Size: 223 2588 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 936 968 1551 1583 2811 Size: 936 32 583 32 1228 Fragments arranged by size: 1228 936 583 32 32 Tth111I GACn'n_nGTC Cuts at: 0 643 1966 2811 Size: 643 1323 845 Fragments arranged by size: 1323 845 643 XbaI T'CTAG_A Cuts at: 0 2511 2811 Size: 2511 300 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 408 916 2811 Size: 408 508 1895 Fragments arranged by size: 1895 508 408 XhoI C'TCGA_G Cuts at: 0 2075 2811 Size: 2075 736 XmaI C'CCGG_G Cuts at: 0 1448 2811 Size: 1448 1363 XmnI GAAnn'nnTTC Cuts at: 0 322 2811 Size: 322 2489 ZraI GAC'GTC Cuts at: 0 2322 2811 Size: 2322 489 Enzymes that do cut: AarI AatII AccI AcuI AfeI AflIII AhdI AleI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I NcoI NspI PciI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PstI PvuII SexAI SfcI SmaI SmlI StyI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AflII AgeI AlfI AloI AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BglII BlpI BmtI BsaBI BsaWI BseYI BsiEI BsiWI BspEI BspHI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstZ17I CspCI DraI DraIII DrdI EagI EciI EcoICRI FseI FspI FspAI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PpiI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI