(Circular) (Six-base) MAPSORT of: pFN21AB5858.seq Check: 1216 from: 1 to: 6175 pFN21AB5858 6175 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2174 2243 2174 Size: 69 6106 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3985 278 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3518 1937 Size: 1581 4594 Acc65I G'GTAC_C Cuts at: 3496 3496 Size: 6175 AclI AA'CG_TT Cuts at: 1563 2775 4302 4675 1563 Size: 1212 1527 373 3063 Fragments arranged by size: 3063 1527 1212 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 2822 2873 4238 5622 5890 1632 Size: 455 735 51 1365 1384 268 1917 Fragments arranged by size: 1917 1384 1365 735 455 268 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5978 AflIII A'CryG_T Cuts at: 1203 2828 5074 6051 1203 Size: 1625 2246 977 1327 Fragments arranged by size: 2246 1625 1327 977 AgeI A'CCGG_T Cuts at: 5006 5006 Size: 6175 AhdI GACnn_n'nnGTC Cuts at: 4904 4904 Size: 6175 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6175 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6175 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3101 3133 3101 Size: 32 6143 AlwNI CAG_nnn'CTG Cuts at: 3918 5490 3918 Size: 1572 4603 ApaI G_GGCC'C Cuts at: 2719 2719 Size: 6175 ApaLI G'TGCA_C Cuts at: 4232 5388 4232 Size: 1156 5019 ApoI r'AATT_y Cuts at: 1493 3368 3484 3710 3862 5971 1493 Size: 1875 116 226 152 2109 1697 Fragments arranged by size: 2109 1875 1697 226 152 116 AseI AT'TA_AT Cuts at: 160 1032 4729 160 Size: 872 3697 1606 Fragments arranged by size: 3697 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 AvaI C'yCGr_G Cuts at: 1870 1943 3500 1870 Size: 73 1557 4545 Fragments arranged by size: 4545 1557 73 AvrII C'CTAG_G Cuts at: 2719 2719 Size: 6175 BamHI G'GATC_C Cuts at: 3505 3872 3505 Size: 367 5808 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2299 2357 3496 4951 618 Size: 325 217 627 512 58 1139 1455 1842 Fragments arranged by size: 1842 1455 1139 627 512 325 217 58 BanII G_rGCy'C Cuts at: 1391 2367 2719 3435 3494 1391 Size: 976 352 716 59 4072 Fragments arranged by size: 4072 976 716 352 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2029 928 Size: 971 130 5074 Fragments arranged by size: 5074 971 130 BbvCI CC'TCA_GC Cuts at: 2046 2046 Size: 6175 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4366 4400 1903 Size: 34 2429 34 3678 Fragments arranged by size: 3678 2429 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2929 4067 5277 2929 Size: 1138 1210 3827 Fragments arranged by size: 3827 1210 1138 BclI T'GATC_A Cuts at: 1548 2560 1548 Size: 1012 5163 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4786 136 Size: 107 122 71 631 605 3114 1525 Fragments arranged by size: 3114 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2727 6170 2727 Size: 3443 2732 BlpI GC'TnA_GC Cuts at: 2109 3065 3592 2109 Size: 956 527 4692 Fragments arranged by size: 4692 956 527 Bme1580I G_kGCm'C Cuts at: 1790 2304 2719 3243 4236 5392 1790 Size: 514 415 524 993 1156 2573 Fragments arranged by size: 2573 1156 993 524 514 415 BmgBI CAC'GTC Cuts at: 1318 2455 1318 Size: 1137 5038 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3188 3906 4864 Size: 416 53 83 399 24 1771 718 958 Cuts at: 4864 442 Size: 1753 Fragments arranged by size: 1771 1753 958 718 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6175 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3130 3130 Size: 6175 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3032 3080 4835 1239 Size: 1793 48 1755 2579 Fragments arranged by size: 2579 1793 1755 48 Bpu10I CC'TnA_GC Cuts at: 2046 2046 Size: 6175 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4300 5165 5463 4300 Size: 865 298 5012 Fragments arranged by size: 5012 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4838 882 Size: 617 3339 2219 Fragments arranged by size: 3339 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2410 3966 493 Size: 711 1206 1556 2702 Fragments arranged by size: 2702 1556 1206 711 BsaBI GATnn'nnATC Cuts at: 6169 6169 Size: 6175 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3982 4364 4999 275 Size: 53 83 186 936 2449 382 635 1451 Fragments arranged by size: 2449 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4608 5006 5280 5427 5838 4608 Size: 398 274 147 411 4945 Fragments arranged by size: 4945 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3014 3044 3101 3131 3275 Size: 30 165 30 1611 30 57 30 144 Cuts at: 3275 3305 1178 Size: 30 4048 Fragments arranged by size: 4048 1611 165 144 57 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2441 2444 2756 3122 1186 Size: 1255 3 312 366 4239 Fragments arranged by size: 4239 1255 366 312 3 BseYI C'CCAG_C Cuts at: 3434 3451 5378 5874 3434 Size: 17 1927 496 3735 Fragments arranged by size: 3735 1927 496 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2809 3350 1163 Size: 573 1073 541 3988 Fragments arranged by size: 3988 1073 573 541 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2230 3625 4386 5414 1540 Size: 275 186 229 1395 761 1028 2301 Fragments arranged by size: 2301 1395 1028 761 275 229 186 BsiHKAI G_wGCw'C Cuts at: 2418 4236 4321 5392 2418 Size: 1818 85 1071 3201 Fragments arranged by size: 3201 1818 1071 85 BsmI GAATG_Cn' Cuts at: 3686 3779 3686 Size: 93 6082 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2304 2367 2418 2719 3243 3435 3494 Size: 399 514 63 51 301 524 192 59 Cuts at: 3494 4236 4321 5392 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 524 514 399 301 192 85 63 59 51 BspEI T'CCGG_A Cuts at: 5838 5838 Size: 6175 BspHI T'CATG_A Cuts at: 2665 2980 4064 5794 2665 Size: 315 1084 1730 3046 Fragments arranged by size: 3046 1730 1084 315 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2174 2243 2451 3409 3530 844 Size: 1330 69 208 958 121 3489 Fragments arranged by size: 3489 1330 958 208 121 69 BsrBI CCG'CTC Cuts at: 1394 1823 2113 4062 1394 Size: 429 290 1949 3507 Fragments arranged by size: 3507 1949 429 290 BsrDI GCAATG_nn' Cuts at: 65 1248 3445 4670 4844 65 Size: 1183 2197 1225 174 1396 Fragments arranged by size: 2197 1396 1225 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4819 5006 1700 Size: 110 3009 187 2869 Fragments arranged by size: 3009 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6175 BssHII G'CGCG_C Cuts at: 1925 2327 1925 Size: 402 5773 BssSI C'ACGA_G Cuts at: 4235 5247 4235 Size: 1012 5163 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6175 BstBI TT'CG_AA Cuts at: 4979 4979 Size: 6175 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6175 BstXI CCAn_nnnn'nTGG Cuts at: 2198 3133 3274 2198 Size: 935 141 5099 Fragments arranged by size: 5099 935 141 BstYI r'GATC_y Cuts at: 1972 2727 3505 3872 4257 4274 5726 5812 5824 Size: 755 778 367 385 17 1452 86 12 Cuts at: 5824 6170 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 778 755 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2388 2580 513 Size: 552 1323 192 4108 Fragments arranged by size: 4108 1323 552 192 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2177 487 Size: 683 56 951 4485 Fragments arranged by size: 4485 951 683 56 BtsI GCAGTG_nn' Cuts at: 2133 2210 2679 2836 3691 4485 4505 2133 Size: 77 469 157 855 794 20 3803 Fragments arranged by size: 3803 855 794 469 157 77 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6175 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6140 DraI TTT'AAA Cuts at: 3479 3479 Size: 6175 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5182 809 Size: 507 3866 1802 Fragments arranged by size: 3866 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2277 2477 3186 Size: 54 1425 50 275 18 447 200 709 Cuts at: 3186 3426 3622 4511 6140 8 Size: 240 196 889 1629 43 Fragments arranged by size: 1629 1425 889 709 447 275 240 200 196 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3622 1537 Size: 275 1810 4090 Fragments arranged by size: 4090 1810 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2870 4105 1353 Size: 1517 1235 3423 Fragments arranged by size: 3423 1517 1235 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3347 4762 5136 5282 387 Size: 1064 138 1758 1415 374 146 1280 Fragments arranged by size: 1758 1415 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2822 2873 3032 3080 4238 4835 Size: 393 455 735 51 159 48 1158 597 Cuts at: 4835 5622 5890 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1158 787 735 597 455 393 268 159 51 48 EcoO109I rG'GnC_Cy Cuts at: 2440 2716 3082 2440 Size: 276 366 5533 Fragments arranged by size: 5533 366 276 EcoRI G'AATT_C Cuts at: 3368 3484 3862 3368 Size: 116 378 5681 Fragments arranged by size: 5681 378 116 EcoRV GAT'ATC Cuts at: 2098 2098 Size: 6175 FspI TGC'GCA Cuts at: 4681 4681 Size: 6175 HaeII r_GCGC'y Cuts at: 1678 5322 1678 Size: 3644 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4816 4848 4890 4922 1373 Size: 32 135 32 3244 32 42 32 2626 Fragments arranged by size: 3244 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3519 3765 669 Size: 988 281 1581 246 3079 Fragments arranged by size: 3079 1581 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6175 HpaI GTT'AAC Cuts at: 3765 3765 Size: 6175 KpnI G_GTAC'C Cuts at: 3500 3500 Size: 6175 MfeI C'AATT_G Cuts at: 3774 3774 Size: 6175 MluI A'CGCG_T Cuts at: 6051 6051 Size: 6175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5289 5473 1305 Size: 3984 184 2007 Fragments arranged by size: 3984 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4353 Fragments arranged by size: 4353 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2196 2265 3050 3188 3233 3326 4133 Size: 648 1030 69 785 138 45 93 807 Cuts at: 4133 4492 4651 518 Size: 359 159 2042 Fragments arranged by size: 2042 1030 807 785 648 359 159 138 93 69 45 MspA1I CmG'CkG Cuts at: 1606 2045 2800 3591 4268 5416 5661 1606 Size: 439 755 791 677 1148 245 2120 Fragments arranged by size: 2120 1148 791 755 677 439 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5623 NdeI CA'TA_TG Cuts at: 387 387 Size: 6175 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6175 NotI GC'GGCC_GC Cuts at: 3622 3622 Size: 6175 NspI r_CATG'y Cuts at: 1132 1229 2506 2832 3055 3221 3355 3533 5078 Size: 97 1277 326 223 166 134 178 1545 Cuts at: 5078 1132 Size: 2229 Fragments arranged by size: 2229 1545 1277 326 223 178 166 134 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6175 PciI A'CATG_T Cuts at: 2828 5074 2828 Size: 2246 3929 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6175 PmeI GTTT'AAAC Cuts at: 3479 3479 Size: 6175 PmlI CAC'GTG Cuts at: 2410 2410 Size: 6175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3101 3133 4244 4276 5757 5789 3101 Size: 32 1111 32 1481 32 3487 Fragments arranged by size: 3487 1481 1111 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2440 3082 2440 Size: 642 5533 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6175 PsiI TTA'TAA Cuts at: 3745 3745 Size: 6175 PspOMI G'GGCC_C Cuts at: 2715 2715 Size: 6175 PstI C_TGCA'G Cuts at: 830 2095 2577 3527 830 Size: 1265 482 950 3478 Fragments arranged by size: 3478 1265 950 482 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6175 PvuII CAG'CTG Cuts at: 2800 2800 Size: 6175 SalI G'TCGA_C Cuts at: 1936 3517 1936 Size: 1581 4594 SbfI CC_TGCA'GG Cuts at: 2577 3527 2577 Size: 950 5225 ScaI AGT'ACT Cuts at: 2474 2813 4423 6135 2474 Size: 339 1610 1712 2514 Fragments arranged by size: 2514 1712 1610 339 SfcI C'TryA_G Cuts at: 826 1045 2091 2573 3523 4658 5339 5530 826 Size: 219 1046 482 950 1135 681 191 1471 Fragments arranged by size: 1471 1135 1046 950 681 482 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6175 SmaI CCC'GGG Cuts at: 1872 3502 1872 Size: 1630 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4279 5180 5442 820 Size: 197 926 2336 901 262 1553 Fragments arranged by size: 2336 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3966 493 Size: 3473 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6175 SphI G_CATG'C Cuts at: 1132 2506 3221 3533 1132 Size: 1374 715 312 3774 Fragments arranged by size: 3774 1374 715 312 SspI AAT'ATT Cuts at: 5 52 4099 5 Size: 47 4047 2081 Fragments arranged by size: 4047 2081 47 StuI AGG'CCT Cuts at: 1517 2571 3101 3113 1517 Size: 1054 530 12 4579 Fragments arranged by size: 4579 1054 530 12 StyI C'CwwG_G Cuts at: 513 1065 2076 2719 2931 3614 513 Size: 552 1011 643 212 683 3074 Fragments arranged by size: 3074 1011 683 643 552 212 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4202 4219 4372 4557 5002 325 Size: 1255 2622 17 153 185 445 1498 Fragments arranged by size: 2622 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2472 2508 2639 Size: 274 80 33 51 1443 495 36 131 Cuts at: 2639 2811 3375 4421 6133 96 Size: 172 564 1046 1712 138 Fragments arranged by size: 1712 1443 1046 564 495 274 172 138 131 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3275 3307 6135 6167 3275 Size: 32 2828 32 3283 Fragments arranged by size: 3283 2828 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2752 1316 Size: 1436 4739 XbaI T'CTAG_A Cuts at: 3511 3511 Size: 6175 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2608 2938 3133 3421 2608 Size: 330 195 288 5362 Fragments arranged by size: 5362 330 288 195 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6175 XmaI C'CCGG_G Cuts at: 1870 3500 1870 Size: 1630 4545 XmnI GAAnn'nnTTC Cuts at: 3368 4304 3368 Size: 936 5239 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3983 276 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI