Restriction map for pFN21AB5897
 (Linear) (Six-base) MAPSORT of: KIBB5897.seq  Check: 3244  from: 1  to: 2511



KIBB5897.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     825    2511 
   Size:        825    1686
 
AccI GT'mk_AC

Cuts at:      0    2066    2511 
   Size:       2066     445
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40      89    1166    1203    1813    2200    2344    2511 
   Size:         40      49    1077      37     610     387     144     167

  Fragments arranged by size: 

                1077     610     387     167     144      49      40      37
            
 
AleI CACnn'nnGTG

Cuts at:      0    2243    2511 
   Size:       2243     268
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     421     434    2511 
   Size:        421      13    2077

  Fragments arranged by size: 

                2077     421      13
 
AlwNI CAG_nnn'CTG

Cuts at:      0      70     142     178     999    2249    2511 
   Size:         70      72      36     821    1250     262

  Fragments arranged by size: 

                1250     821     262      72      70      36
 
ApaI G_GGCC'C

Cuts at:      0     188    2511 
   Size:        188    2323
 
ApoI r'AATT_y

Cuts at:      0    1072    2511 
   Size:       1072    1439
 
AvaI C'yCGr_G

Cuts at:      0     187     743    1036    1454    2070    2511 
   Size:        187     556     293     418     616     441

  Fragments arranged by size: 

                 616     556     441     418     293     187
 
BamHI G'GATC_C

Cuts at:      0     222    2511 
   Size:        222    2289
 
BanI G'GyrC_C

Cuts at:      0     132    1032    1896    2511 
   Size:        132     900     864     615

  Fragments arranged by size: 

                 900     864     615     132
 
BanII G_rGCy'C

Cuts at:      0     188     770    2078    2511 
   Size:        188     582    1308     433

  Fragments arranged by size: 

                1308     582     433     188
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     315    1722    2332    2511 
   Size:        315    1407     610     179

  Fragments arranged by size: 

                1407     610     315     179
 
BbvCI CC'TCA_GC

Cuts at:      0     845    2511 
   Size:        845    1666
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     483    2511 
   Size:        483    2028
 
BclI T'GATC_A

Cuts at:      0    1658    2511 
   Size:       1658     853
 
BfrBI ATG'CAT

Cuts at:      0     623    2511 
   Size:        623    1888
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1450    2511 
   Size:       1450    1061
 
BglII A'GATC_T

Cuts at:      0    1097    2511 
   Size:       1097    1414
 
BlpI GC'TnA_GC

Cuts at:      0    2077    2511 
   Size:       2077     434
 
Bme1580I G_kGCm'C

Cuts at:      0     188     905    1037    1901    2511 
   Size:        188     717     132     864     610

  Fragments arranged by size: 

                 864     717     610     188     132
 
BmgBI CAC'GTC

Cuts at:      0     852    2511 
   Size:        852    1659
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     188     816    2237    2511 
   Size:        188     628    1421     274

  Fragments arranged by size: 

                1421     628     274     188
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1108    1345    1495    2482    2511 
   Size:       1108     237     150     987      29

  Fragments arranged by size: 

                1108     987     237     150      29
 
Bpu10I CC'TnA_GC

Cuts at:      0     241     845    2511 
   Size:        241     604    1666

  Fragments arranged by size: 

                1666     604     241
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     829    2470    2511 
   Size:        328     501    1641      41

  Fragments arranged by size: 

                1641     501     328      41
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     842    2511 
   Size:        842    1669
 
BsaAI yAC'GTr

Cuts at:      0     471     900    2361    2511 
   Size:        471     429    1461     150

  Fragments arranged by size: 

                1461     471     429     150
 
BsaHI Gr'CG_yC

Cuts at:      0    1525    2511 
   Size:       1525     986
 
BsaWI w'CCGG_w

Cuts at:      0     965    2511 
   Size:        965    1546
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      69      99     475     505    2511 
   Size:         69      30     376      30    2006

  Fragments arranged by size: 

                2006     376      69      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1105    1108    1492    2511 
   Size:       1105       3     384    1019

  Fragments arranged by size: 

                1105    1019     384       3
 
BseYI C'CCAG_C

Cuts at:      0      63      94     147     159     516    1204    1225    1416 
   Size:         63      31      53      12     357     688      21     191

Cuts at:   1416    2511 
   Size:       1095

  Fragments arranged by size: 

                1095     688     357     191      63      53      31      21
                  12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      74     374     736    1211    2511 
   Size:         74     300     362     475    1300

  Fragments arranged by size: 

                1300     475     362     300      74
 
BsiHKAI G_wGCw'C

Cuts at:      0     455     770    2511 
   Size:        455     315    1741

  Fragments arranged by size: 

                1741     455     315
 
BsmI GAATG_Cn'

Cuts at:      0    2129    2511 
   Size:       2129     382
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1420    2511 
   Size:       1420    1091
 
Bsp1286I G_dGCh'C

Cuts at:      0     188     455     770     905    1037    1901    2078    2511 
   Size:        188     267     315     135     132     864     177     433

  Fragments arranged by size: 

                 864     433     315     267     188     177     135     132
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     576     825    1746    2225    2396    2511 
   Size:        576     249     921     479     171     115

  Fragments arranged by size: 

                 921     576     479     249     171     115
 
BsrBI CCG'CTC

Cuts at:      0     215     750    2340    2511 
   Size:        215     535    1590     171

  Fragments arranged by size: 

                1590     535     215     171
 
BsrFI r'CCGG_y

Cuts at:      0     163     989    1538    2511 
   Size:        163     826     549     973

  Fragments arranged by size: 

                 973     826     549     163
 
BssHII G'CGCG_C

Cuts at:      0     255     294    2511 
   Size:        255      39    2217

  Fragments arranged by size: 

                2217     255      39
 
BssSI C'ACGA_G

Cuts at:      0    1558    2511 
   Size:       1558     953
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     702    2511 
   Size:        702    1809
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1371    2460    2511 
   Size:       1371    1089      51

  Fragments arranged by size: 

                1371    1089      51
 
BstYI r'GATC_y

Cuts at:      0     222     402    1097    2054    2105    2511 
   Size:        222     180     695     957      51     406

  Fragments arranged by size: 

                 957     695     406     222     180      51
 
Bsu36I CC'TnA_GG

Cuts at:      0    1604    2511 
   Size:       1604     907
 
BtgI C'CryG_G

Cuts at:      0    1364    1485    2511 
   Size:       1364     121    1026

  Fragments arranged by size: 

                1364    1026     121
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2385    2511 
   Size:       2385     126
 
BtsI GCAGTG_nn'

Cuts at:      0     386    2511 
   Size:        386    2125
 
EaeI y'GGCC_r

Cuts at:      0    1174    1442    2381    2511 
   Size:       1174     268     939     130

  Fragments arranged by size: 

                1174     939     268     130
 
EarI CTCTTCn'nnn_

Cuts at:      0     550     560     607    1036    1291    2511 
   Size:        550      10      47     429     255    1220

  Fragments arranged by size: 

                1220     550     429     255      47      10
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    2511 
   Size:         19    2492
 
EcoICRI GAG'CTC

Cuts at:      0     768    2511 
   Size:        768    1743
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40      89    1108    1166    1203    1345    1495    1813 
   Size:         40      49    1019      58      37     142     150     318

Cuts at:   1813    2200    2344    2482    2511 
   Size:        387     144     138      29

  Fragments arranged by size: 

                1019     387     318     150     144     142     138      58
                  49      40      37      29
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1286    2511 
   Size:       1286    1225
 
EcoO109I rG'GnC_Cy

Cuts at:      0      18     184     656    1046    1420    1772    2184    2283 
   Size:         18     166     472     390     374     352     412      99

Cuts at:   2283    2511 
   Size:        228

  Fragments arranged by size: 

                 472     412     390     374     352     228     166      99
                  18
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     798    2511 
   Size:        798    1713
 
FspI TGC'GCA

Cuts at:      0      41    2511 
   Size:         41    2470
 
HaeII r_GCGC'y

Cuts at:      0    1485    2459    2511 
   Size:       1485     974      52

  Fragments arranged by size: 

                1485     974      52
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     105     137     457     475     489     507    2511 
   Size:        105      32     320      18      14      18    2004

  Fragments arranged by size: 

                2004     320     105      32      18      18      14
 
HindIII A'AGCT_T

Cuts at:      0     304     805    2511 
   Size:        304     501    1706

  Fragments arranged by size: 

                1706     501     304
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     142    2258    2511 
   Size:        106      36    2116     253

  Fragments arranged by size: 

                2116     253     106      36
 
MslI CAynn'nnrTG

Cuts at:      0    1363    1894    2243    2511 
   Size:       1363     531     349     268

  Fragments arranged by size: 

                1363     531     349     268
 
MspA1I CmG'CkG

Cuts at:      0     151     418     996    1164    1195    1487    1590    2081 
   Size:        151     267     578     168      31     292     103     491

Cuts at:   2081    2394    2447    2511 
   Size:        313      53      64

  Fragments arranged by size: 

                 578     491     313     292     267     168     151     103
                  64      53      31
 
NaeI GCC'GGC

Cuts at:      0     165     991    2511 
   Size:        165     826    1520

  Fragments arranged by size: 

                1520     826     165
 
NcoI C'CATG_G

Cuts at:      0    1364    2511 
   Size:       1364    1147
 
NgoMIV G'CCGG_C

Cuts at:      0     163     989    2511 
   Size:        163     826    1522

  Fragments arranged by size: 

                1522     826     163
 
NruI TCG'CGA

Cuts at:      0    2414    2511 
   Size:       2414      97
 
NsiI A_TGCA'T

Cuts at:      0     625    2511 
   Size:        625    1886
 
NspI r_CATG'y

Cuts at:      0    1602    1701    2511 
   Size:       1602      99     810

  Fragments arranged by size: 

                1602     810      99
 
PasI CC'CwG_GG

Cuts at:      0     660    1710    2511 
   Size:        660    1050     801

  Fragments arranged by size: 

                1050     801     660
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2249    2511 
   Size:       2249     262
 
PmlI CAC'GTG

Cuts at:      0     900    2511 
   Size:        900    1611
 
PpuMI rG'GwC_Cy

Cuts at:      0     656    1046    1772    2184    2511 
   Size:        656     390     726     412     327

  Fragments arranged by size: 

                 726     656     412     390     327
 
PspOMI G'GGCC_C

Cuts at:      0     184    2511 
   Size:        184    2327
 
PstI C_TGCA'G

Cuts at:      0     156     410     632    1272    1571    2393    2511 
   Size:        156     254     222     640     299     822     118

  Fragments arranged by size: 

                 822     640     299     254     222     156     118
 
PvuII CAG'CTG

Cuts at:      0     151     418     996    1164    1590    2081    2511 
   Size:        151     267     578     168     426     491     430

  Fragments arranged by size: 

                 578     491     430     426     267     168     151
 
SacI G_AGCT'C

Cuts at:      0     770    2511 
   Size:        770    1741
 
SacII CC_GC'GG

Cuts at:      0    1488    2511 
   Size:       1488    1023
 
SanDI GG'GwC_CC

Cuts at:      0     656    1772    2511 
   Size:        656    1116     739

  Fragments arranged by size: 

                1116     739     656
 
ScaI AGT'ACT

Cuts at:      0    2440    2511 
   Size:       2440      71
 
SfcI C'TryA_G

Cuts at:      0     152     406     628    1268    1567    2389    2511 
   Size:        152     254     222     640     299     822     122

  Fragments arranged by size: 

                 822     640     299     254     222     152     122
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1450    2511 
   Size:       1450    1061
 
SmaI CCC'GGG

Cuts at:      0     189     745    1456    2072    2511 
   Size:        189     556     711     616     439

  Fragments arranged by size: 

                 711     616     556     439     189
 
SmlI C'TyrA_G

Cuts at:      0     307     808    2485    2511 
   Size:        307     501    1677      26

  Fragments arranged by size: 

                1677     501     307      26
 
SphI G_CATG'C

Cuts at:      0    1602    2511 
   Size:       1602     909
 
SspI AAT'ATT

Cuts at:      0     690    2511 
   Size:        690    1821
 
StuI AGG'CCT

Cuts at:      0    2498    2511 
   Size:       2498      13
 
StyI C'CwwG_G

Cuts at:      0     458    1364    1529    1883    2511 
   Size:        458     906     165     354     628

  Fragments arranged by size: 

                 906     628     458     354     165
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     108    1222    1342    1723    2511 
   Size:        108    1114     120     381     788

  Fragments arranged by size: 

                1114     788     381     120     108
 
TatI w'GTAC_w

Cuts at:      0     719    2417    2438    2511 
   Size:        719    1698      21      73

  Fragments arranged by size: 

                1698     719      73      21
 
XmaI C'CCGG_G

Cuts at:      0     187     743    1454    2070    2511 
   Size:        187     556     711     616     441

  Fragments arranged by size: 

                 711     616     556     441     187
 
XmnI GAAnn'nnTTC

Cuts at:      0     544    2470    2511 
   Size:        544    1926      41

  Fragments arranged by size: 

                1926     544      41
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI     AlfI    AlwNI     ApaI     ApoI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI     FspI    HaeII    Hin4I  HindIII     MmeI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NruI     NsiI     NspI     PasI
    PflMI     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII
    SanDI     ScaI     SfcI     SfiI     SmaI     SmlI     SphI     SspI
     StuI     StyI    TaqII     TatI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
     BcgI     BmtI     BplI    BsaBI    BsiEI    BsiWI    BspEI    BspHI
    BsrDI    BsrGI    BstBI   BstEII  BstZ17I     ClaI    CspCI     DraI
   DraIII     DrdI     EagI    EcoRI    EcoRV     FseI    FspAI   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NarI     NdeI
     NheI     NotI     PacI     PciI     PfoI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SalI     SapI     SbfI
    SexAI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI
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