(Circular) (Six-base) MAPSORT of: pFN21AB7886.seq Check: 5723 from: 1 to: 6658 pFN21AB7886 6658 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4468 278 Size: 53 83 186 936 2932 2468 Fragments arranged by size: 2932 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4001 1937 Size: 2064 4594 Acc65I G'GTAC_C Cuts at: 3979 3979 Size: 6658 AclI AA'CG_TT Cuts at: 1563 4785 5158 1563 Size: 3222 373 3063 Fragments arranged by size: 3222 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2207 2823 3446 3530 3725 3783 4721 6105 Size: 575 616 623 84 195 58 938 1384 Cuts at: 6105 6373 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 938 623 616 575 268 195 84 58 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6461 AflIII A'CryG_T Cuts at: 1203 2027 2558 5557 6534 1203 Size: 824 531 2999 977 1327 Fragments arranged by size: 2999 1327 977 824 531 AgeI A'CCGG_T Cuts at: 5489 5489 Size: 6658 AhdI GACnn_n'nnGTC Cuts at: 5387 5387 Size: 6658 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6658 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6658 AlwNI CAG_nnn'CTG Cuts at: 2519 3469 3613 4401 5973 2519 Size: 950 144 788 1572 3204 Fragments arranged by size: 3204 1572 950 788 144 ApaLI G'TGCA_C Cuts at: 4715 5871 4715 Size: 1156 5502 ApoI r'AATT_y Cuts at: 1493 2285 2548 2636 2730 3248 3472 3719 3967 Size: 792 263 88 94 518 224 247 248 Cuts at: 3967 4193 4345 6454 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 792 518 263 248 247 226 224 152 94 88 AseI AT'TA_AT Cuts at: 160 1032 5212 160 Size: 872 4180 1606 Fragments arranged by size: 4180 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6658 AvaI C'yCGr_G Cuts at: 1870 1943 2478 3983 1870 Size: 73 535 1505 4545 Fragments arranged by size: 4545 1505 535 73 BamHI G'GATC_C Cuts at: 3988 4355 3988 Size: 367 6291 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 3979 5434 618 Size: 325 217 627 224 1968 1455 1842 Fragments arranged by size: 1968 1842 1455 627 325 224 217 BanII G_rGCy'C Cuts at: 1391 3923 3977 1391 Size: 2532 54 4072 Fragments arranged by size: 4072 2532 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3565 3756 928 Size: 971 1666 191 3830 Fragments arranged by size: 3830 1666 971 191 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4849 4883 1903 Size: 34 2912 34 3678 Fragments arranged by size: 3678 2912 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4550 5760 4550 Size: 1210 5448 BclI T'GATC_A Cuts at: 1548 2484 1548 Size: 936 5722 BfrBI ATG'CAT Cuts at: 2777 2777 Size: 6658 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5269 136 Size: 107 122 71 631 605 337 3260 1525 Fragments arranged by size: 3260 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3283 3654 6653 3283 Size: 371 2999 3288 Fragments arranged by size: 3288 2999 371 BlpI GC'TnA_GC Cuts at: 4075 4075 Size: 6658 Bme1580I G_kGCm'C Cuts at: 1790 3846 4719 5875 1790 Size: 2056 873 1156 2573 Fragments arranged by size: 2573 2056 1156 873 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6658 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2100 3942 4389 Size: 416 53 83 399 24 683 1842 447 Cuts at: 4389 5347 442 Size: 958 1753 Fragments arranged by size: 1842 1753 958 683 447 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6658 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2756 3657 5318 1239 Size: 1517 901 1661 2579 Fragments arranged by size: 2579 1661 1517 901 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2740 2744 4783 5648 5946 2740 Size: 4 2039 865 298 3452 Fragments arranged by size: 3452 2039 865 298 4 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5321 882 Size: 617 3822 2219 Fragments arranged by size: 3822 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4449 493 Size: 711 3245 2702 Fragments arranged by size: 3245 2702 711 BsaBI GATnn'nnATC Cuts at: 2535 3033 3050 3866 6652 2535 Size: 498 17 816 2786 2541 Fragments arranged by size: 2786 2541 816 498 17 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4465 4847 5482 275 Size: 53 83 186 936 2932 382 635 1451 Fragments arranged by size: 2932 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5091 5489 5763 5910 6321 5091 Size: 398 274 147 411 5428 Fragments arranged by size: 5428 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3633 3663 1178 Size: 30 165 30 2230 30 4173 Fragments arranged by size: 4173 2230 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3654 1186 Size: 2468 4190 BseYI C'CCAG_C Cuts at: 2074 5861 6357 2074 Size: 3787 496 2375 Fragments arranged by size: 3787 2375 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3287 3643 3671 1163 Size: 573 1551 356 28 4150 Fragments arranged by size: 4150 1551 573 356 28 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 4108 4869 5897 1540 Size: 275 186 48 2059 761 1028 2301 Fragments arranged by size: 2301 2059 1028 761 275 186 48 BsiHKAI G_wGCw'C Cuts at: 4719 4804 5875 4719 Size: 85 1071 5502 Fragments arranged by size: 5502 1071 85 BsmI GAATG_Cn' Cuts at: 2779 3341 4169 4262 2779 Size: 562 828 93 5175 Fragments arranged by size: 5175 828 562 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3846 3923 3977 4719 4804 5875 1391 Size: 399 2056 77 54 742 85 1071 2174 Fragments arranged by size: 2174 2056 1071 742 399 85 77 54 BspEI T'CCGG_A Cuts at: 6321 6321 Size: 6658 BspHI T'CATG_A Cuts at: 4547 6277 4547 Size: 1730 4928 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4013 844 Size: 3169 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3432 3481 4545 1394 Size: 429 1609 49 1064 3507 Fragments arranged by size: 3507 1609 1064 429 49 BsrDI GCAATG_nn' Cuts at: 65 1248 5153 5327 65 Size: 1183 3905 174 1396 Fragments arranged by size: 3905 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5302 5489 1700 Size: 110 3492 187 2869 Fragments arranged by size: 3492 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3094 3936 96 Size: 2998 842 2818 Fragments arranged by size: 2998 2818 842 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6658 BssSI C'ACGA_G Cuts at: 4718 5730 4718 Size: 1012 5646 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6658 BstBI TT'CG_AA Cuts at: 2650 5462 2650 Size: 2812 3846 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6658 BstYI r'GATC_y Cuts at: 1972 2242 3028 3283 3654 3988 4355 4740 4757 Size: 270 786 255 371 334 367 385 17 Cuts at: 4757 6209 6295 6307 6653 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 786 385 371 367 346 334 270 255 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2606 2841 3361 513 Size: 552 939 602 235 520 3810 Fragments arranged by size: 3810 939 602 552 520 235 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2514 3363 487 Size: 683 56 1288 849 3782 Fragments arranged by size: 3782 1288 849 683 56 BtsI GCAGTG_nn' Cuts at: 4174 4968 4988 4174 Size: 794 20 5844 Fragments arranged by size: 5844 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6658 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2610 2645 3897 3915 3932 3950 529 Size: 35 2046 35 1252 18 17 18 3237 Fragments arranged by size: 3237 2046 1252 35 35 18 18 17 DraI TTT'AAA Cuts at: 3962 3962 Size: 6658 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5665 809 Size: 507 4349 1802 Fragments arranged by size: 4349 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 4105 4994 Size: 54 1425 50 275 18 216 2059 889 Cuts at: 4994 6623 8 Size: 1629 43 Fragments arranged by size: 2059 1629 1425 889 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 4105 1537 Size: 275 234 2059 4090 Fragments arranged by size: 4090 2059 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 2379 3701 3759 4588 1353 Size: 1026 1322 58 829 3423 Fragments arranged by size: 3423 1322 1026 829 58 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5245 5619 5765 387 Size: 1064 138 3656 374 146 1280 Fragments arranged by size: 3656 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2756 2823 3446 3530 3657 3725 Size: 393 575 549 67 623 84 127 68 Cuts at: 3725 3783 4721 5318 6105 6373 1239 Size: 58 938 597 787 268 1524 Fragments arranged by size: 1524 938 787 623 597 575 549 393 268 127 84 68 67 58 EcoO109I rG'GnC_Cy Cuts at: 3178 3178 Size: 6658 EcoRI G'AATT_C Cuts at: 2285 3967 4345 2285 Size: 1682 378 4598 Fragments arranged by size: 4598 1682 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2370 2370 Size: 6658 FspI TGC'GCA Cuts at: 5164 5164 Size: 6658 HaeII r_GCGC'y Cuts at: 1678 2944 5805 1678 Size: 1266 2861 2531 Fragments arranged by size: 2861 2531 1266 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2430 2462 2911 2943 3586 Size: 32 135 32 858 32 449 32 643 Cuts at: 3586 3618 5299 5331 5373 5405 1373 Size: 32 1681 32 42 32 2626 Fragments arranged by size: 2626 1681 858 643 449 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3295 3535 4002 4248 669 Size: 988 281 1357 240 467 246 3079 Fragments arranged by size: 3079 1357 988 467 281 246 240 HindIII A'AGCT_T Cuts at: 748 3229 748 Size: 2481 4177 HpaI GTT'AAC Cuts at: 3295 3535 4248 3295 Size: 240 713 5705 Fragments arranged by size: 5705 713 240 KpnI G_GTAC'C Cuts at: 3983 3983 Size: 6658 MfeI C'AATT_G Cuts at: 4257 4257 Size: 6658 MluI A'CGCG_T Cuts at: 2027 6534 2027 Size: 4507 2151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2738 2745 5772 5956 1305 Size: 1433 7 3027 184 2007 Fragments arranged by size: 3027 2007 1433 184 7 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4836 Fragments arranged by size: 4836 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2671 2954 4616 4975 5134 518 Size: 648 1505 283 1662 359 159 2042 Fragments arranged by size: 2042 1662 1505 648 359 283 159 MspA1I CmG'CkG Cuts at: 1606 2236 3635 3670 4074 4751 5899 6144 1606 Size: 630 1399 35 404 677 1148 245 2120 Fragments arranged by size: 2120 1399 1148 677 630 404 245 35 NcoI C'CATG_G Cuts at: 513 1065 2004 3361 513 Size: 552 939 1357 3810 Fragments arranged by size: 3810 1357 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6658 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6658 NotI GC'GGCC_GC Cuts at: 2046 4105 2046 Size: 2059 4599 NruI TCG'CGA Cuts at: 3039 3039 Size: 6658 NsiI A_TGCA'T Cuts at: 2779 2779 Size: 6658 NspI r_CATG'y Cuts at: 1132 1229 2562 3943 4016 5561 1132 Size: 97 1333 1381 73 1545 2229 Fragments arranged by size: 2229 1545 1381 1333 97 73 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6658 PciI A'CATG_T Cuts at: 2558 5557 2558 Size: 2999 3659 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6658 PmeI GTTT'AAAC Cuts at: 3962 3962 Size: 6658 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4727 4759 6240 6272 4727 Size: 32 1481 32 5113 Fragments arranged by size: 5113 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3178 3178 Size: 6658 PshAI GACnn'nnGTC Cuts at: 1613 2177 2559 1613 Size: 564 382 5712 Fragments arranged by size: 5712 564 382 PsiI TTA'TAA Cuts at: 4228 4228 Size: 6658 PstI C_TGCA'G Cuts at: 830 2065 4010 830 Size: 1235 1945 3478 Fragments arranged by size: 3478 1945 1235 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6658 PvuII CAG'CTG Cuts at: 2236 2236 Size: 6658 SalI G'TCGA_C Cuts at: 1936 4000 1936 Size: 2064 4594 SapI GCTCTTCn'nnn_ Cuts at: 3759 3759 Size: 6658 SbfI CC_TGCA'GG Cuts at: 4010 4010 Size: 6658 ScaI AGT'ACT Cuts at: 4906 6618 4906 Size: 1712 4946 SfcI C'TryA_G Cuts at: 826 1045 2061 2931 3118 3770 4006 5141 5822 Size: 219 1016 870 187 652 236 1135 681 Cuts at: 5822 6013 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1016 870 681 652 236 219 191 187 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6658 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6658 SmaI CCC'GGG Cuts at: 1872 3985 1872 Size: 2113 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2719 2759 4762 5663 5925 820 Size: 197 926 776 40 2003 901 262 1553 Fragments arranged by size: 2003 1553 926 901 776 262 197 40 SnaBI TAC'GTA Cuts at: 493 4449 493 Size: 3956 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6658 SphI G_CATG'C Cuts at: 1132 4016 1132 Size: 2884 3774 SspI AAT'ATT Cuts at: 5 52 4582 5 Size: 47 4530 2081 Fragments arranged by size: 4530 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6658 StyI C'CwwG_G Cuts at: 513 1065 2004 2354 3361 4097 513 Size: 552 939 350 1007 736 3074 Fragments arranged by size: 3074 1007 939 736 552 350 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3092 4685 4702 4855 5040 5485 325 Size: 1255 1512 1593 17 153 185 445 1498 Fragments arranged by size: 1593 1512 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2190 2304 3094 Size: 274 80 33 51 1443 213 114 790 Cuts at: 3094 3936 4904 6616 96 Size: 842 968 1712 138 Fragments arranged by size: 1712 1443 968 842 790 274 213 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3633 3665 6618 6650 3633 Size: 32 2953 32 3641 Fragments arranged by size: 3641 2953 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2442 1316 Size: 1126 5532 XbaI T'CTAG_A Cuts at: 3994 3994 Size: 6658 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2604 2604 Size: 6658 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6658 XmaI C'CCGG_G Cuts at: 1870 3983 1870 Size: 2113 4545 XmnI GAAnn'nnTTC Cuts at: 2637 3904 4787 2637 Size: 1267 883 4508 Fragments arranged by size: 4508 1267 883 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4466 276 Size: 53 83 186 936 2932 2468 Fragments arranged by size: 2932 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI