(Circular) (Six-base) MAPSORT of: pFN21AB5718.seq Check: 3038 from: 1 to: 6877 pFN21AB5718 6877 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4687 278 Size: 53 83 186 936 3151 2468 Fragments arranged by size: 3151 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4220 1937 Size: 2283 4594 Acc65I G'GTAC_C Cuts at: 4198 4198 Size: 6877 AclI AA'CG_TT Cuts at: 1563 5004 5377 1563 Size: 3441 373 3063 Fragments arranged by size: 3441 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2745 2859 3478 4940 6324 6592 1632 Size: 1113 114 619 1462 1384 268 1917 Fragments arranged by size: 1917 1462 1384 1113 619 268 114 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6680 AflIII A'CryG_T Cuts at: 1203 3080 5776 6753 1203 Size: 1877 2696 977 1327 Fragments arranged by size: 2696 1877 1327 977 AgeI A'CCGG_T Cuts at: 5708 5708 Size: 6877 AhdI GACnn_n'nnGTC Cuts at: 5606 5606 Size: 6877 AleI CACnn'nnGTG Cuts at: 1166 3846 1166 Size: 2680 4197 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6877 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3453 3485 3453 Size: 32 6845 AlwNI CAG_nnn'CTG Cuts at: 3110 3533 4620 6192 3110 Size: 423 1087 1572 3795 Fragments arranged by size: 3795 1572 1087 423 ApaLI G'TGCA_C Cuts at: 4934 6090 4934 Size: 1156 5721 ApoI r'AATT_y Cuts at: 1493 2191 3603 4186 4412 4564 6673 1493 Size: 698 1412 583 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 698 583 226 152 AseI AT'TA_AT Cuts at: 160 1032 5431 160 Size: 872 4399 1606 Fragments arranged by size: 4399 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6877 AvaI C'yCGr_G Cuts at: 1870 1943 3768 4113 4202 1870 Size: 73 1825 345 89 4545 Fragments arranged by size: 4545 1825 345 89 73 BamHI G'GATC_C Cuts at: 2539 4207 4574 2539 Size: 1668 367 4842 Fragments arranged by size: 4842 1668 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4198 5653 618 Size: 325 217 627 2411 1455 1842 Fragments arranged by size: 2411 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4196 1391 Size: 2805 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3897 4097 928 Size: 971 1998 200 3708 Fragments arranged by size: 3708 1998 971 200 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3126 3160 5068 5102 1903 Size: 34 1189 34 1908 34 3678 Fragments arranged by size: 3678 1908 1189 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3098 3137 4769 5979 3098 Size: 39 1632 1210 3996 Fragments arranged by size: 3996 1632 1210 39 BclI T'GATC_A Cuts at: 1548 2155 3942 1548 Size: 607 1787 4483 Fragments arranged by size: 4483 1787 607 BfrBI ATG'CAT Cuts at: 2078 2078 Size: 6877 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5488 136 Size: 107 122 71 631 605 3816 1525 Fragments arranged by size: 3816 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3251 3743 3979 6872 3251 Size: 492 236 2893 3256 Fragments arranged by size: 3256 2893 492 236 BlpI GC'TnA_GC Cuts at: 4294 4294 Size: 6877 Bme1580I G_kGCm'C Cuts at: 1790 2752 4938 6094 1790 Size: 962 2186 1156 2573 Fragments arranged by size: 2573 2186 1156 962 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6877 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3731 4608 5566 Size: 416 53 83 399 24 2314 877 958 Cuts at: 5566 442 Size: 1753 Fragments arranged by size: 2314 1753 958 877 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6877 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3783 3783 Size: 6877 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2142 2755 3417 5537 1239 Size: 903 613 662 2120 2579 Fragments arranged by size: 2579 2120 903 662 613 Bpu10I CC'TnA_GC Cuts at: 4036 4036 Size: 6877 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2529 3195 3557 5002 5867 6165 2529 Size: 666 362 1445 865 298 3241 Fragments arranged by size: 3241 1445 865 666 362 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5540 882 Size: 617 4041 2219 Fragments arranged by size: 4041 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2747 3155 4016 4668 493 Size: 711 1543 408 861 652 2702 Fragments arranged by size: 2702 1543 861 711 652 408 BsaBI GATnn'nnATC Cuts at: 6871 6871 Size: 6877 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4684 5066 5701 275 Size: 53 83 186 936 3151 382 635 1451 Fragments arranged by size: 3151 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2030 2277 5310 5708 5982 6129 6540 2030 Size: 247 3033 398 274 147 411 2367 Fragments arranged by size: 3033 2367 411 398 274 247 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2048 2078 2464 2494 1178 Size: 30 165 30 645 30 386 30 5561 Fragments arranged by size: 5561 645 386 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2101 2321 2324 2472 3465 1186 Size: 915 220 3 148 993 4598 Fragments arranged by size: 4598 993 915 220 148 3 BseYI C'CCAG_C Cuts at: 6080 6576 6080 Size: 496 6381 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6304 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4327 5088 6116 1540 Size: 275 186 2326 761 1028 2301 Fragments arranged by size: 2326 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4938 5023 6094 4938 Size: 85 1071 5721 Fragments arranged by size: 5721 1071 85 BsmI GAATG_Cn' Cuts at: 2529 3468 4388 4481 2529 Size: 939 920 93 4925 Fragments arranged by size: 4925 939 920 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2752 4196 4938 5023 6094 1391 Size: 399 962 1444 742 85 1071 2174 Fragments arranged by size: 2174 1444 1071 962 742 399 85 BspEI T'CCGG_A Cuts at: 2030 6540 2030 Size: 4510 2367 BspHI T'CATG_A Cuts at: 3058 4766 6496 3058 Size: 1708 1730 3439 Fragments arranged by size: 3439 1730 1708 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2793 4232 844 Size: 1949 1439 3489 Fragments arranged by size: 3489 1949 1439 BsrBI CCG'CTC Cuts at: 1394 1823 2010 4764 1394 Size: 429 187 2754 3507 Fragments arranged by size: 3507 2754 429 187 BsrDI GCAATG_nn' Cuts at: 65 1248 2650 5372 5546 65 Size: 1183 1402 2722 174 1396 Fragments arranged by size: 2722 1402 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5521 5708 1700 Size: 110 3711 187 2869 Fragments arranged by size: 3711 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6877 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6877 BssSI C'ACGA_G Cuts at: 3231 4937 5949 3231 Size: 1706 1012 4159 Fragments arranged by size: 4159 1706 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6877 BstBI TT'CG_AA Cuts at: 5681 5681 Size: 6877 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6877 BstYI r'GATC_y Cuts at: 1972 2125 2371 2539 3251 3743 3979 4207 4574 Size: 153 246 168 712 492 236 228 367 Cuts at: 4574 4959 4976 6428 6514 6526 6872 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 712 492 385 367 346 246 236 228 168 153 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3558 3622 513 Size: 552 2493 64 3768 Fragments arranged by size: 3768 2493 552 64 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6138 Fragments arranged by size: 6138 683 56 BtsI GCAGTG_nn' Cuts at: 2113 3838 4036 4051 4393 5187 5207 2113 Size: 1725 198 15 342 794 20 3783 Fragments arranged by size: 3783 1725 794 342 198 20 15 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6877 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6842 DraI TTT'AAA Cuts at: 2190 3183 3286 4181 2190 Size: 993 103 895 4886 Fragments arranged by size: 4886 993 895 103 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5884 809 Size: 507 4568 1802 Fragments arranged by size: 4568 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2110 3035 4324 Size: 54 1425 50 275 18 280 925 1289 Cuts at: 4324 5213 6842 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1289 925 889 280 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4324 1537 Size: 275 2512 4090 Fragments arranged by size: 4090 2512 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2078 2632 3781 3846 4160 4807 1353 Size: 725 554 1149 65 314 647 3423 Fragments arranged by size: 3423 1149 725 647 554 314 65 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5464 5838 5984 387 Size: 1064 138 3875 374 146 1280 Fragments arranged by size: 3875 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2745 2755 2859 3417 3478 4940 Size: 393 510 603 10 104 558 61 1462 Cuts at: 4940 5537 6324 6592 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1462 787 603 597 558 510 393 268 104 61 10 EcoNI CCTnn'n_nnAGG Cuts at: 3135 3135 Size: 6877 EcoRI G'AATT_C Cuts at: 4186 4564 4186 Size: 378 6499 EcoRV GAT'ATC Cuts at: 2263 2263 Size: 6877 FspI TGC'GCA Cuts at: 5383 5383 Size: 6877 HaeII r_GCGC'y Cuts at: 1678 3692 6024 1678 Size: 2014 2332 2531 Fragments arranged by size: 2531 2332 2014 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2450 2482 3437 3469 3735 Size: 32 135 32 878 32 955 32 266 Cuts at: 3735 3767 4084 4116 5518 5550 5592 5624 1373 Size: 32 317 32 1402 32 42 32 2626 Fragments arranged by size: 2626 1402 955 878 317 266 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2201 2767 3303 4081 4221 4467 Size: 988 281 263 566 536 778 140 246 Cuts at: 4467 669 Size: 3079 Fragments arranged by size: 3079 988 778 566 536 281 263 246 140 HindIII A'AGCT_T Cuts at: 748 748 Size: 6877 HpaI GTT'AAC Cuts at: 4467 4467 Size: 6877 KpnI G_GTAC'C Cuts at: 4202 4202 Size: 6877 MfeI C'AATT_G Cuts at: 4476 4476 Size: 6877 MluI A'CGCG_T Cuts at: 6753 6753 Size: 6877 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2384 2960 5991 6175 1305 Size: 1079 576 3031 184 2007 Fragments arranged by size: 3031 2007 1079 576 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5055 Fragments arranged by size: 5055 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3846 4835 5194 5353 518 Size: 648 2680 989 359 159 2042 Fragments arranged by size: 2680 2042 989 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2411 4293 4970 6118 6363 1606 Size: 805 1882 677 1148 245 2120 Fragments arranged by size: 2120 1882 1148 805 677 245 NcoI C'CATG_G Cuts at: 513 1065 3622 513 Size: 552 2557 3768 Fragments arranged by size: 3768 2557 552 NdeI CA'TA_TG Cuts at: 387 3486 387 Size: 3099 3778 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6877 NotI GC'GGCC_GC Cuts at: 4324 4324 Size: 6877 NsiI A_TGCA'T Cuts at: 2080 2080 Size: 6877 NspI r_CATG'y Cuts at: 1132 1229 2606 2912 4235 5780 1132 Size: 97 1377 306 1323 1545 2229 Fragments arranged by size: 2229 1545 1377 1323 306 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6877 PciI A'CATG_T Cuts at: 5776 5776 Size: 6877 PflMI CCAn_nnn'nTGG Cuts at: 1108 2942 1108 Size: 1834 5043 PfoI T'CCnGG_A Cuts at: 3433 3433 Size: 6877 PmeI GTTT'AAAC Cuts at: 4181 4181 Size: 6877 PmlI CAC'GTG Cuts at: 2747 2747 Size: 6877 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4946 4978 6459 6491 4946 Size: 32 1481 32 5332 Fragments arranged by size: 5332 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6877 PsiI TTA'TAA Cuts at: 2781 4447 2781 Size: 1666 5211 PstI C_TGCA'G Cuts at: 830 4229 830 Size: 3399 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6877 PvuII CAG'CTG Cuts at: 2411 2411 Size: 6877 SalI G'TCGA_C Cuts at: 1936 4219 1936 Size: 2283 4594 SbfI CC_TGCA'GG Cuts at: 4229 4229 Size: 6877 ScaI AGT'ACT Cuts at: 2836 5125 6837 2836 Size: 2289 1712 2876 Fragments arranged by size: 2876 2289 1712 SexAI A'CCwGG_T Cuts at: 2941 2941 Size: 6877 SfcI C'TryA_G Cuts at: 826 1045 3132 4225 5360 6041 6232 826 Size: 219 2087 1093 1135 681 191 1471 Fragments arranged by size: 2087 1471 1135 1093 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6877 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6877 SmaI CCC'GGG Cuts at: 1872 4115 4204 1872 Size: 2243 89 4545 Fragments arranged by size: 4545 2243 89 SmlI C'TyrA_G Cuts at: 820 1017 1943 2544 3210 3572 3768 4981 5882 Size: 197 926 601 666 362 196 1213 901 Cuts at: 5882 6144 820 Size: 262 1553 Fragments arranged by size: 1553 1213 926 901 666 601 362 262 197 196 SnaBI TAC'GTA Cuts at: 493 4668 493 Size: 4175 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6877 SphI G_CATG'C Cuts at: 1132 2606 4235 1132 Size: 1474 1629 3774 Fragments arranged by size: 3774 1629 1474 SspI AAT'ATT Cuts at: 5 52 2144 4801 5 Size: 47 2092 2657 2081 Fragments arranged by size: 2657 2092 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6877 StyI C'CwwG_G Cuts at: 513 1065 2985 3622 4095 4316 513 Size: 552 1920 637 473 221 3074 Fragments arranged by size: 3074 1920 637 552 473 221 SwaI ATTT'AAAT Cuts at: 2190 2190 Size: 6877 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2465 3078 4904 4921 5074 5259 5704 Size: 1255 885 613 1826 17 153 185 445 Cuts at: 5704 325 Size: 1498 Fragments arranged by size: 1826 1498 1255 885 613 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2834 5123 6835 Size: 274 80 33 51 1443 857 2289 1712 Cuts at: 6835 96 Size: 138 Fragments arranged by size: 2289 1712 1443 857 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2872 2904 6837 6869 2872 Size: 32 3933 32 2880 Fragments arranged by size: 3933 2880 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6877 XbaI T'CTAG_A Cuts at: 4213 4213 Size: 6877 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2690 3172 2690 Size: 482 6395 XhoI C'TCGA_G Cuts at: 1943 3768 1943 Size: 1825 5052 XmaI C'CCGG_G Cuts at: 1870 4113 4202 1870 Size: 2243 89 4545 Fragments arranged by size: 4545 2243 89 XmnI GAAnn'nnTTC Cuts at: 3316 3402 3640 5006 3316 Size: 86 238 1366 5187 Fragments arranged by size: 5187 1366 238 86 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4685 276 Size: 53 83 186 936 3151 2468 Fragments arranged by size: 3151 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI